基于马氏珠母贝(Pinctada fucata)血细胞全长转录组数据补体样组分的鉴定与分析     

王菁  李桂英  林楷琪  欧静  张淑瓶  王忠良 《海洋与湖沼》2022,53(1):176-186

补体系统作为先天免疫的重要组成部分,是一种复杂的限制性蛋白水解系统,其在免疫系统中发挥着重要的防御作用。为分析马氏珠母贝补体系统的组成及作用机制,使用血细胞样品进行了全长转录组测序建库、基因比对、功能注释,共挖掘到212个潜在补体样组分相关基因。补体样组分基因经同源性比对和结构域检测分析表明,检索到的基因分别编码89个含C1q结构域蛋白、57个C型凝集素蛋白、33个纤维胶凝蛋白、11个纤维蛋白原相关蛋白、8个甘露糖结合型凝集素关联丝氨酸蛋白酶、2个含硫酯蛋白(1个C3分子,1个TEP分子)、1个补体受体、2个补体因子、9个丝氨酸蛋白酶。随机选择12个补体相关基因,使用溶藻弧菌刺激前后的血细胞样品进行实时定量PCR检测其表达水平,结果显示C1q(C1q domain containing protein)、C-lectin、MBL(mannose-binding lectin)、ficolin、MASP(mannan-binding lectin serine protease)等基因均呈现出显著差异表达,表明马氏珠母贝补体系统是一个复杂的多组分效应系统,且可能通过凝集素途径或类似于凝集素途径激活补体系统的免疫作用。研究结果为进一步验证马氏珠母贝中存在的原始补体系统提供了分子生物学证据,同时对深入了解马氏珠母贝免疫防御机制,丰富和发展海洋无脊椎动物免疫学内容也具有重要理论意义。  相似文献   

青蛤(Cyclina sinensis)TLR2基因的克隆与表达分析   总被引：2，自引：2，他引：0  

任毅鹏  高晶  潘宝平  高虹  闫春财 《海洋与湖沼》2014,45(5):1037-1043

利用转录组文库分析得到青蛤(Cyclina sinensis)的Toll样受体2(Toll-like receptor 2,TLR2)基因序列,采用荧光定量PCR法分析该基因的表达过程。结果显示,青蛤TLR2基因的cDNA开放阅读框为2082bp,编码693个氨基酸,具典型的LRRs、单次跨膜区和TIR结构域。该基因在血淋巴中的表达量最高,与肝脏、鳃、外套膜、闭壳肌和性腺有显著性差异(P0.05)。在鳗弧菌和Poly I:C刺激下,青蛤血淋巴中TLR2基因3h开始升高,48h达到最大值,与对照组和空白组有极显著性差异(P0.01);藤黄微球菌刺激下,血淋巴中CsTLR2基因3h开始升高,48h亦达到最大值,实验组与对照组有显著性差异(P0.05)。  相似文献   

凡纳滨对虾(Litopenaeus vannamei)Runt转录因子的基因克隆及其功能分析     

王晓雯  梁艳  邢婧  刘旭雅  黄倢 《海洋与湖沼》2014,45(6):1338-1345

Runt结构域转录因子家族(Runx)在哺乳动物体内存在三种基因型,分别起着造血,骨生成和控制上皮细胞增殖的作用。本文应用反转录和c DNA末端快速扩增(RACE)技术,首次在凡纳滨对虾(Litopenaeus vannamei)中克隆并测序了一种Runt(lvrunt)基因全长。lvrunt c DNA全长1754bp,含有1个663bp长的开放阅读框,编码221个氨基酸,理论分子量为23.6k Da,具有Runt-family结构域。对凡纳滨对虾鳃、肠、肝胰腺、类淋巴、心脏、肌肉组织和血淋巴lvrunt的转录特征进行分析,结果显示该基因几乎特异性地在血淋巴细胞中表达,而在其他组织中表达较低。肌肉注射重组造血激素蛋白(r AST)或白斑综合征病毒(WSSV)粗提液均可显著提高lvrunt在凡纳滨对虾体内的转录表达。上述结果提示,lvrunt主要在血细胞发挥作用,可能作为造血激素的下游基因起到调节血细胞增殖和分化的作用,并在受到病毒感染后,参与提升血细胞数量或者促进血细胞分化的过程。  相似文献   

Screening and molecular classification of low-temperature protease from Antarctic microorganism and its characterization     

王全富缪锦来  侯艳华郑洲 李光友 《极地研究(英文版)》2005,16(2):81-89

107 strains producing protease were screened from 260 strains of Antarctic psychrophilic bacteria, among which proteolytic activity of five strains was more than 45 U ml^-1. The 16S rRNA gcne sequences homology and phylogcnetic analysis of five Antarctic psychrophillc bacteria showed that NJ276, NJS-9, NJ16-70,NJ345 belonged tO the described genus Pseudoalteromonas and NJ341 belonged to the genus Colwellia. The growth and the protease characteristic of four Antarctic psychrophilic bacteria had been studied, and the result showed that the 6ptimal temperature for growth and protease-produeing of four strains was about 10℃. Their growth and protease-produeing were still high during incubatlng 2-5 days. The maximum proteolytic activity occurred at pH 9 for four Antarctic psychrophilic bacteria. The optimal temperature of protease action of both strains NJ276 and NJ5-9 was about 50℃, however, the optimal temperature of protease aetlon of both strains NJ341 and NJ345 was about 40 ℃, and their proteolytic activity under 0℃ exhibited nearly 30% of the maximum activity, but their thermal stabilities were weaker. These results indicated that proteases from NJ341 and NJ345 were low-temperature proteases.  相似文献   

强磁场对中子星壳层热核反应的影响     

罗志全  彭秋和 《中国天文和天体物理学报》1996,(4)

本文通过考虑强磁场中电子的量子效应，分析了强磁场下电子气体的Fermi能，讨论了磁场对核的屏蔽势和核反应率的影响，进而计算了强磁场对天体物理中几个较重要的热核反应的影响，结果表明，相对于无磁场而言，在较低密度下，足够强的磁场使原子核的屏蔽势显著增加，但在ρ／μe＞10~5molcm~(-3)的密度下，中子星表面存在的强度为10~5～10~9T范围内的磁场对核反应率几乎没有影响。  相似文献   

恒星内部电荷屏蔽对电子俘获反应的影响   总被引：1，自引：0，他引：1  

罗志全  彭秋和  戴子高 《天文学报》1997,(1)

本文分析了在恒星环境下，电荷强屏蔽对原子核电子俘获反应的影响，分析中忽略了电荷屏蔽对核跃迁矩阵元的影响．作为例子，分别讨论了电荷屏蔽对核素33S、30P和57Fe的电子俘获率的影响．结果表明：在较低温度和较高密度下，由于电荷屏蔽，其电子俘获率相对于无屏蔽情形有明显降低，这可能对恒星晚期演化和超新星爆发理论带来明显的影响．  相似文献   

Fast tidal cycling and the origin of life     

Richard Lathe 《Icarus》2004,168(1):18-22

Replicating prebiotic polymers are thought to predate the emergence of true life-forms. The initial mode of replication, a prerequisite for Darwinian selection, is unknown, but demands an explanation based on local physicochemistry. Dual consideration of the conditions of the early terrestrial surface, with the unusual physicochemical properties of nucleic acids like DNA, could explain the emergence of nucleic acids as key biomolecules. The early impact that produced the Moon, and fast terrestrial rotation, subjected coastal areas 3.9 Ga ago to rapid tidal flooding (dilution) and drying (concentration), with a likely periodicity in the range of 2-6 h, and could have provided a driving force for cyclic replication of early biomolecules. Such a mechanism applies only to molecules capable of association/polymerization at high salt concentration, and of dissociation at low salinity. Nucleic acids meet these criteria. It is suggested that tidal cycling, resembling the polymerase chain reaction (PCR) mechanism, could only replicate and amplify DNA-like polymers. This mechanism suggests constraints on the evolution of extra-terrestrial life.  相似文献   

Transformation of ammonium nitrogen and response characteristics of nitrifying functional genes in tannery sludge contaminated soil          下载免费PDF全文

Kong Xiang-ke  Zhang Zi-xuan  Wang Ping  Wang Yan-yan  Zhang Zhao-ji  Han Zhan-tao  Ma Li-sha 《地下水科学与工程》2022,10(3):223-232

High concentrations of ammonium nitrogen released from tannery sludge during storage in open air may cause nitrogen pollution to soil and groundwater. To study the transformation mechanism of NH₄⁺-N by nitrifying functional bacteria in tannery sludge contaminated soils, a series of contaminated soil culture experiments were conducted in this study. The contents of ammonium nitrogen (as NH₄⁺-N), nitrite nitrogen (as NO₂^?-N) and nitrate nitrogen (as NO₃^?-N) were analyzed during the culture period under different conditions of pollution load, soil particle and redox environment. Sigmodial equation was used to interpret the change of NO₃^?-N with time in contaminated soils. The abundance variations of nitrifying functional genes (amoA and nxrA) were also detected using the real-time quantitative fluorescence PCR method. The results show that the nitrification of NH₄⁺-N was aggravated in the contaminated silt soil and fine sand under the condition of lower pollution load, finer particle size and more oxidizing environment. The sigmodial equation well fitted the dynamic accumulation curve of the NO₃^?-N content in the tannery sludge contaminated soils. The Cr(III) content increased with increasing pollution load, which inhibited the reproduction and activity of nitrifying bacteria in the soils, especially in coarse-grained soil. The accumulation of NO₂^?-N contents became more obvious with the increase of pollution load in the fine sand, and only 41.5% of the NH₄⁺-N was transformed to NO₃^?-N. The redox environment was the main factor affecting nitrification process in the soil. Compared to the aerobic soil environment, the transformation of NH₄⁺-N was significantly inhibited under anaerobic incubation condition, and the NO₃^?-N contents decreased by 37.2%, 61.9% and 91.9% under low, medium and high pollution loads, respectively. Nitrification was stronger in the silt soil since its copy number of amoA and nxrA genes was two times larger than that of fine sand. Moreover, the copy numbers of amoA and nxrA genes in the silt soil under the aerobic environment were 2.7 times and 2.2 times larger than those in the anaerobic environment. The abundance changes of the amoA and nxrA functional genes have a positive correlation with the nitrification intensity in the tannery sludge-contaminated soil.  相似文献   

重庆多历时极值降水的概率型优选及应用     

王颖  刘晓冉  程炳岩  孙佳 《气象科学》2020,40(4):560-568

基于重庆34个国家气象站1981—2016年5 min,24 h至3 d的过程最大雨量共18个年极值降水序列,利用线性矩法计算6种常用概率分布函数的模型参数,通过备选最优模型筛选法客观选取各站各历时极值降水的最优概率模型,并将优选结果应用于重庆不同历时百年重现期降水的计算。结果表明:模型优选方法得到的重庆各站不同历时极值降水的最优线型略有差异,广义极值分布占比最大,三参数Weibull分布次之,三参数对数正态分布第三,皮尔逊Ⅲ型和Gumbel分布相当,指数分布最差。最优线型计算的重庆不同历时百年重现期降水的空间分布大值区由短历时的点状分布向长历时的片状分布转变,渝东北的大值中心受地形影响不断向北移动。基于线性矩法的概率模型参数估计及客观的线型优选过程具有较强的可操作性和适应性,可应用于其他工程气象参数的推算中。  相似文献   

Electronic effects in astrophysically relevant nuclear reactions     

H. Leeb  H. Oberhummer 《Astrophysics and Space Science》1995,228(1-2):87-105

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