溶藻弧菌对大黄鱼表皮黏液的黏附特性研究   总被引：3，自引：0，他引：3  

鄢庆枇  陈强  马甡  庄峙厦  王小如 《海洋学报》2006,28(6):100-105

采用3H-TdR同位素示踪方法研究了溶藻弧菌对大黄鱼表皮黏液的黏附机制.试验结果表明溶藻弧菌经营养饥饿、热处理、抗体处理、蛋白酶及高碘酸处理后黏附作用明显下降;在8种碳水化合物的干扰下溶藻弧菌的黏附作用会有一定程度的变化,其中葡萄糖、果糖、甘露糖能明显促进溶藻弧菌的黏附作用;溶藻弧菌对大黄鱼表皮黏液中较大分子量的物质有较强的亲和力.这些结果表明溶藻弧菌对大黄鱼表皮黏液存在特异性黏附作用,这种黏附与细菌活力、其表面的蛋白类、糖类等物质以及黏液的化学组成都有密切关系.  相似文献   

Tyrosinase, a new innate humoral immune parameter in large yellow croaker （Pseudosciaena crocea R）     

王淑红  王艺磊  张子平  谢芳靖  林鹏  邰振刚 《中国海洋湖沼学报》2009,27(3):527-535

We evaluated the immune response to infection with a pathogen in large yellow croaker(Pseudosciaena crocea Richardson).The fish were given an intraperitoneal(i.p.) injection of Vibrio parahaemolyticus or sterile sea water(control).We collected blood sera from the fish 0.17,1,2,4,8,12,or 16 d after injection(dpi).We measured tyrosinase activity and the concentrations of lysozyme,NOS,and antibodies.Serum tyrosinase activity was significantly higher at 0.17 and 4 dpi than in the control group,and peaked at 8 d...  相似文献   

Immune response of <Emphasis Type="Italic">Pseudosciaena crocea</Emphasis> to the injection of <Emphasis Type="Italic">Vibrio alginolyticus</Emphasis>     

鄢庆柔  张俊杰  邹文政  陈强  庄峙厦  王小如 《中国海洋湖沼学报》2009,27(1):85-91

For the investigation of anti-infection immune response of Pseudosciaena crocea, 160 healthy fish samples were categorized into infected and control groups. Each individual fish in the infected group was injected intraperitoneally (i.p.) with 0.2 ml bacterial suspension of Vibrio alginolyticus in density of 2×10⁷ CFU/ml, while each individual in the control group was injected i.p. with 0.2 ml sterile saline solution (0.85%). It was observed that the artificial injection of V. alginolyticus significantly increased the number of erythrocytes, leucocytes, lymphocytes in peripheral blood as well as peripheral serum antibacterial activity and antibody titer of large yellow croaker, and significantly reduced the number of peripheral blood granulocytes as compared with those in the control group. No significant difference in acid phosphytase and superoxide dismutase activity of serum was detected between the two groups. It is suggested that non-specific immune factors including leucocytes and anti-bacteria substance in peripheral blood played important role at the initial stage of infection, and specific immune factors such as antibody then played important role in response to anti-infection at the latter stage. Supported by the National High Technology Research and Development Program of China (863 program, No. 2007AA09Z115) and Technology Program of Xiamen (No. 3502Z73019)  相似文献   

东南沿海几种经济鱼类肌肉组织学比较研究   总被引：1，自引：0，他引：1  

徐斌  苏永全  张纹  郭明兰  王军 《海洋科学》2009,33(7):33-36

采用石蜡组织切片的方法,对大黄鱼(Pseudosciaena crocea)5个品系以及斜带髭鲷(Hapalogenys nitens)、牙鲆(Paralichthys olivaceus)、青石斑鱼(Epinephelus awoara),尼罗罗非鱼(Tilapia nilotica)和条纹斑竹鲨(Chiloscyllium plagiosum)等鱼类肌肉纤维组织学特性的研究表明:野生大黄鱼与野生养殖品系肌肌肉纤维在组织学上没有明显差异,而雌核发育品系及其杂交后代则相对野生品系表现出显著差异,它们的肌肉纤维更细密;不同种鱼之间肌肌肉纤维在组织学表现出巨大的差异,是与各个种类的遗传特征和生存模式相适应的.  相似文献   

大黄鱼肌肉生长抑制素基因原核表达及多克隆抗体的制备     

董海阳  叶秀丽  薛良义  Diallo Amadou 《台湾海峡》2011,30(2):196-202

肌肉生长抑制素抑制动物肌肉的生长发育.根据本实验室克隆的大黄鱼（Pseudosciaena cro-cea）肌肉生长抑制素（MSTN）基因编码序列设计引物,用RT-PCR扩增目的片段,构建MSTN-pET-28 a重组表达质粒,将其转化到大肠杆菌BL21上并用1.0 mmol/dm3IPTG诱导表达,SDS-PAGE电泳检测该目的蛋白大小约为44 kDa,与理论值大小符合.用0.25 mol/dm3KCl染色后从凝胶中切取该蛋白且回收.该回收蛋白与弗氏佐剂等量混合后注射ICR小鼠制备多克隆抗体,并用Westernblot检测该抗体.免疫印迹结果在44 kDa的位置上出现棕色条带,表明大黄鱼肌肉生长抑制素的多克隆抗体制备成功.这为今后对肌肉生长抑制素功能的研究奠定了基础.  相似文献   

大黄鱼（Pseudosciaena crocea）一种新型抗菌肽的纯化及其生物学特性研究          下载免费PDF全文

纪勇  王晓露  苏永全  覃映雪  邹文政  邢颜丽  徐晓津  鄢庆枇 《海洋与湖沼》2011,42(3):448-454

以致病性溶藻弧菌为指示菌,通过酸粗提、固相萃取、Sephadex G-25柱凝胶层析和RP—HPLC等分离纯化技术,从大黄鱼胃肠组织中分离纯化抗菌肽。结果表明,大黄鱼肠道组织中存在着多种抗菌物质,并最终得到一种高纯度的新型抗菌肽AMP-W3。AMP—W3由10种氨基酸组成,其中天冬氨酸、谷氨酸和甘氨酸的含量较高。AMP...  相似文献   

A Fast and Indriect Fluorescent Antibody Assay for the Vibro in Large Yeelow Croaker Pseudosciaena Crocea (Richardson)     

王军  鄢庆枇 《中国海洋湖沼学报》2003,21(1):91-96

A fast and indirect fluorescnet antibody assay for the Vibrio alginolyticus and V.Parahaemolyticus infecting the large yellow croaker has been developed.The specific antisera for the two strains of vibrio were prepared with New Zealand rabbit and the antiserum and cross-reactive efficacy was tested by coagulation in tube.It showed that the goat anti-rabbit IgG had been labeled by fuorescence isothiocyanate(FITC).The results showed that positive reactions were 100% for the large yellow croaker Pseudosciaena crocea with typical symptom of vibrio infection,while the positive reaction to the pathogen in healthy yellow croakers reached 40%,but seemed negative for aquaculter water.The results demonstrated that this fast and indirect fluorescent antibody assay can be used not only to test the vibrio pathogen in diseased yellow croaker but also in infected animals with no symptom.  相似文献   

A Fast and Indirect Fluorescent Antibody Assay for the Vibrio in Large Yellow Croaker Pseudosciaena crocea (Richardson)     

王军  苏永全  鄢庆枇 《中国海洋湖沼学报》2003,21(1)

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大黄鱼(Pseudosciaena crocea)岱衢洋选育群体和官井洋养殖群体的遗传差异分析   总被引：8，自引：0，他引：8  

丁诗华  黄丽英  张海琪  徐晓林 《海洋与湖沼》2006,37(1):41-46

应用RAPD技术对大黄鱼岱衢洋选育群体和官井洋养殖群体的遗传差异进行研究,选取15条10bp随机引物,共检测出96个RAPD位点。结果表明,岱衢洋选育群体的多态位点比例为33.33%,平均杂合度为0.3008,Shannon多样性信息指数为0.0879,群体内个体间平均遗传相似系数为0.9079,平均遗传距离为0.0921;官井洋养殖群体的多态位点比例为43.75%,平均杂合度为0.3298,Shannon信息指数为0.1221,群体内个体间平均遗传相似系数为0.8784,平均遗传距离为0.1216。大部分遗传变异(93.52%)存在于群体内,少部分遗传变异(6.48%)存在于群体间。两群体间的Nei氏标准遗传距离为0.0123。以上分析表明,大黄鱼官井洋养殖群体的遗传多样性水平高于岱衢洋选育群体,但两个群体都处于较低的遗传多样性水平,需要对大黄鱼的种质资源进行科学管理和保护,以避免遗传多样性下降。  相似文献   

Identification and characterization of adult alpha-and beta-globin genes and their genomic arrangement in Pseudosciaena crocea     

CHU Wuying  QIAN Ronghu  WANG Lianshen  YU Xiameng  YOU Zhenqiang  YU Lian 《海洋学报(英文版)》2006,25(4):65-76

1Introduction Hemoglobin(Hb)playsanimportantrolein oxygentransportinmanyorganisms.Amongverte brates,Hbisatetramericmoleculethatconsistsof twoαglobinandtwoβglobinchains.Eachofthe fourglobinchainsiscoordinatedwithahemegroup thatfunctionsdirectlyforoxygent…  相似文献