真鲷脂蛋白脂肪酶基因顺式元件PPRE及在肝脏活体调控作用   总被引：7，自引：0，他引：7  

梁旭方  李月琴  李贵生  白俊杰  劳海华  Hiromi Oku  Hiroshi Y.OGATA 《热带海洋学报》2004,23(4):49-55

从血液提取总DNA并构建基因组文库,克隆海水鱼真鲷(Pagrosomusmajor)脂蛋白脂肪酶(LPL)基因5′侧翼区序列,再通过竞争性聚合酶链式反应(PCR)方法,定量研究饲料中添加不同饱和度脂肪酸对其肝脏LPL基因mRNA表达水平的影响。结果表明,真鲷LPL基因5′侧翼区序列中存在顺式元件过氧化物酶体增殖物反应元件,其序列为TGAAGA TGACAT,与TATA(CATAA)盒序列相隔211bp;与喂饲料对照组比较,添加10%油酸可增加真鲷肝脏LPL基因表达水平,但差异并不显著(p>0.05)。用亚油酸或n 3高不饱和脂肪酸混合物取代添加油酸的一半(占饲料添加量的5%)后,导致真鲷肝脏LPL基因表达水平升高并出现显著性变化(p<0.05)。对真鲷肝脏LPL基因诱导表达随饲料中的脂肪酸不饱和度增加而增加,表明在活体条件下脂肪酸对真鲷肝脏LPL基因表达的诱导作用可能与脂肪酸的氧化代谢有关。n 3高不饱和脂肪酸通过过氧化物酶体增殖物激活受体诱导真鲷肝脏LPL基因表达,使真鲷食物脂质供应水平与其肝脏脂肪酸氧化代谢强度调控偶联起来,可能是真鲷对高脂食物(特别是富含n 3高不饱和脂肪酸食物)的一种适应。  相似文献   

大型多管水母的绿色荧光蛋白   总被引：3，自引：0，他引：3  

罗文新  张军  杨海杰  谢小燕  覃映雪  逄淑强  李少菁  夏宁邵 《海洋学报》2002,24(4):82-91

采用PCR扩增和southern杂交筛选相结合的方法,从厦门水域的大型多管水母中分离到了新的绿色荧光蛋白基因gfpxm,并在大肠杆菌中进行了表达.gfpxmDNA序列编码区长为1042bp,包含3个外显子和两个内含子,cDNA编码区全长为717bp.gfpxmcDNA与已知野生型Aeqgfp 10cDNA长度一致,核苷酸同源性为81.9%,推导的氨基酸序列全长为238个氨基酸,与AeqGFP10的氨基酸同源性为83.6%.将gfpxm克隆至pTO-T7表达载体,GFPxm在大肠杆菌BL21中的表达量达菌体总蛋白的50%左右.荧光性质和强度分析结果表明,GFPxm蛋白的激发峰为476nm,发射峰为496nm,荧光量子产率为1. GFPxm蛋白的荧光很稳定,对热、碱性、变性剂和盐等有较强抗性.  相似文献   

海滨香豌豆(Lathyrus maritimus Bigel)核型分析研究     

陈登勤  韩宝芹  杨震 《中国海洋大学学报(自然科学版)》1992,(4)

以海滨香豌豆根尖为材料,用压片法研究了其染色体和核型,结果表明,海滨香豌豆核型为2n=14=6m+8sm。与栽培豌豆染色体数目相同,核型相似。  相似文献   

3种不同来源的鲑鱼降钙素基因的克隆及其序列比较     

张宝红  茅云翔  王高歌  杨官品  张学成 《中国海洋大学学报(自然科学版)》2002,32(4):585-590

以 3种不同来源的鲑鱼基因组 DNA为模板 ,采用 PCR方法获得完整的 s CT基因。与Genbank中 Pink Salmon(Oncorhynchus gorbuscha)的降钙素基因序列进行比较 ,结果显示 :实验组的 Pink Salmon(O.gorbuscha)降钙素基因有 2个碱基差异 (第 39位 C→ A;第 5 1位 T→ C) ,氨基酸没有变化 ;Chum Salmon(O.keta)有 1个碱基的差异 (第 86位 G→ A) ,且引起 1个氨基酸的变化(第 2 9位 S→ N) ;而中国黑龙江产的鲑鱼 (Oncorhynchus sp.)降钙素基因序列与 Genbank中 PinkSalmon(O.gorbuscha)的降钙素序列完全相同 ,没有碱基差别。  相似文献   

角蛋白酶基因工程研究   总被引：2，自引：0，他引：2  

韩晓宇  刘亚洁  李江 《华东地质学院学报》2007,30(3):287-290

角蛋白是一种广泛存在于自然界中的硬性蛋白,主要存在于各种动物的毛发、鳞片、羽毛、蹄、角等结构中。角蛋白化学结构稳定,不易被一般的蛋白酶降解,但作为角蛋白酶的专一性底物,角蛋白可被角蛋白酶降解。角蛋白酶由微生物产生,可特异性降解羽毛等含角蛋白类的物质,在食品、医药、饲料生产和环境废物处理等方面具有潜在的应用价值。综述了微生物角蛋白酶基因分离和表达研究进展,并对角蛋白酶基因在异源表达过程中出现的问题进行了讨论。  相似文献   

耐旱藓类的抗旱生理及其机理研究   总被引：15，自引：8，他引：15  

吴玉环  程佳强  冯虎元  安黎哲  高谦  程国栋 《中国沙漠》2004,24(1):23-29

耐旱藓类广泛分布于干旱及半干旱地区。本文综述分析了苔藓植物的耐旱能力和适应耐旱的形态结构和生理特征，从细胞和分子水平，对苔藓植物的耐旱机理进行了综述，并展望了我国苔藓植物的耐旱生理生态学研究前景。  相似文献   

An application of artificial intelligence for rainfall-runoff modeling   总被引：5，自引：0，他引：5  

Ali Aytek  M. Asce  Murat Alp 《Journal of Earth System Science》2008,117(2):145-155

This study proposes an application of two techniques of artificial intelligence (AI) for rainfall-runoff modeling: the artificial neural networks (ANN) and the evolutionary computation (EC). Two different ANN techniques, the feed forward back propagation (FFBP) and generalized regression neural network (GRNN) methods are compared with one EC method, Gene Expression Programming (GEP) which is a new evolutionary algorithm that evolves computer programs. The daily hydrometeorological data of three rainfall stations and one streamflow station for Juniata River Basin in Pennsylvania state of USA are taken into consideration in the model development. Statistical parameters such as average, standard deviation, coefficient of variation, skewness, minimum and maximum values, as well as criteria such as mean square error (MSE) and determination coefficient (R ²) are used to measure the performance of the models. The results indicate that the proposed genetic programming (GP) formulation performs quite well compared to results obtained by ANNs and is quite practical for use. It is concluded from the results that GEP can be proposed as an alternative to ANN models.  相似文献   

Evaluation of gene expression programming approaches for estimating daily evaporation through spatial and temporal data scanning   总被引：1，自引：0，他引：1       下载免费PDF全文

Jalal Shiri  Pau Marti  Vijay P. Singh 《水文研究》2014,28(3):1215-1225

Evaporation of water from free water surfaces or from land surfaces is one of the main components of the hydrological cycle, and its occurrence is governed by various meteorological and physical factors. There is a multitude of models developed for estimating daily evaporation values by using weather data. This paper evaluates a Gene Expression Programming (GEP) model for estimating evaporation through spatial and temporal data scanning techniques. It is by using ‘leave‐one‐out’ procedures, a complete scan of the possible train and test set configurations is carried out according to temporal and spatial criteria. Comparison of the GEP model with empirical‐physical models shows that daily evaporation values computed by the GEP model are more accurate. Further, local calibration of the GEP model may not be needed, if enough climatic data are available at other stations. The performance of the GEP model fluctuates throughout the period of study and between stations. A suitable assessment of the model should consider a complete temporal and/or spatial scan of the data set used. Copyright © 2012 John Wiley & Sons, Ltd.  相似文献   

Comparative molecular analysis of bacterial communities inhabiting pristine and polluted with polycyclic aromatic hydrocarbons Black Sea coastal sediments     

Nadezhda H. Todorova  Roumyana S. Mironova  Ventzislav K. Karamfilov 《Marine pollution bulletin》2014

Molecular analysis was applied to characterize bacterial community structure in sediment samples collected from pristine site and oil-polluted Black Sea harbor. Amplified Ribosomal DNA Restriction Analysis (ARDRA) revealed a high similarity in the restriction patterns of both samples thus not demonstrating the effect of the pollutant on the structure of the bacterial communities. Constructed 16S rRNA gene libraries gave more detailed assessment of members. Results showed that α- and γ-Proteobacteria were dominant in the oil polluted site, whereas the pristine site was characterized by prevalence of Actinobacteria. The biodegradative potential of the adapted bacterial community in the oil-polluted sediments was demonstrated by the presence of the aromatic ring hydroxylating dioxygenase genes.  相似文献   

Molecular cloning and characterization of SoxB2 gene from Zhikong scallop Chlamys farreri     

贺艳  包振民  郭慧慧  张月月  张玲玲  王师  胡景杰  胡晓丽 《中国海洋湖沼学报》2013,31(6):1216-1225

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