基于皮肤转录组的半滑舌鳎无眼侧黑化相关基因的筛选鉴定     

赵娜  何晓旭  贾磊  朱春华  张博 《海洋学报》2022,44(2):57-66

半滑舌鳎(Cynoglossus semilaevis)作为比目鱼(Pleuronectiformes)中的代表物种,是我国沿海地区重要的海水经济鱼类.半滑舌鳎体色异常的问题长期以来一直困扰着从业者.导致体色变化的原因多种多样,遗传因素是最根本原因.针对半滑舌鳎无眼侧黑化的研究多在环境、营养、生理、常见色素基因克隆等方...  相似文献   

嵌岩桩负摩阻力现场试验与计算方法研究     

吴爽爽  胡新丽  章涵  周昌  龚辉 《岩土力学》2019,40(9):3610-3617

桩-土剪切位移对桩基负摩阻力发展有重要的影响。在既有研究的基础上,通过桩周土体沉降与桩身应变的监测结果建立了计算桩-土剪切位移量的方法。利用该方法对浅中层桩-土监测数据进行分析,确定了在桩顶自由条件下该试验场地桩-土剪切位移量在4~5 mm;对于深层桩-土没有达到该剪切位移量,负摩阻力则无法发挥至极值。根据负摩阻力的发挥特征,改进了规范中嵌岩桩负摩阻力的计算方法,该方法同时考虑了桩周土竖向有效应力与桩土剪切位移的影响。使用该方法计算的负摩阻力与实测结果较为吻合,获得的下拉荷载能够达到安全与经济的效果,可供工程设计参考、借鉴。  相似文献   

Diseases and parasites of Baltic cod (Gadus morhua) from the Mecklenburg Bight to the Estonian coast     

Mellergaard  S.; Lang  T. 《ICES Journal of Marine Science》1999,56(2):164-168

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Recovery of atypical Aeromonas salmonicida from ulcerated fish from the Baltic Sea     

Wiklund  T.; Tabolina  I.; Bezgachina  T. V. 《ICES Journal of Marine Science》1999,56(2):175-179

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大黄鱼病原副溶血弧菌单克隆抗体制备及其应用   总被引：5，自引：0，他引：5  

池信才  王军  鄢庆枇  苏永全 《海洋科学》2007,31(8):1-5

用甲醛灭活副溶血弧菌(Vibrio parahaemolyticus)和溶藻弧菌(V.alginolyticus)制成免疫原免疫BALB/c小鼠,利用淋巴细胞杂交瘤技术,获得1株特异的针对副溶血弧菌的单克隆抗体,命名为D6F3H5。腹水及培养上清液抗体的ELISA效价分别为:1∶5 120和1∶1 280,该单克隆抗体与其它细菌没有明显的交叉反应。利用该单克隆抗体和兔抗副溶血弧菌多克隆抗体,建立了检测副溶血弧菌的双抗体夹心ELISA方法。该方法对副溶血弧菌的最小检出量为5×104个/mL。用双抗体夹心ELISA检测大黄鱼(Pseudosciaena crocea)样品,14尾患病大黄鱼中有11尾检出副溶血弧菌,而10尾健康大黄鱼都没有检出副溶血弧菌。由此可见,本试验制备的高特异性的抗副溶血弧菌单克隆抗体,可以用于患病大黄鱼副溶血弧菌的快速诊断。  相似文献   

不同堆载形式对群桩负摩阻力的影响     

马学宁  付江  王军  王旭 《岩土力学》2018,39(10):3531-3538

在既有桥墩桩基础周围土体堆载时,会引起土体的沉降和侧向变形,进而在桩身产生负摩阻力,对桩基变形及承载性能有很大影响。为研究围载和单侧边载作用下群桩中不同位置桩基的受力差异,以3×3群桩基础为研究对象,进行围载和单侧边载作用下的模型试验,分析了不同位置桩基轴力、侧摩阻力、中性点位置和基桩承载力安全系数等的变化规律及差异。研究结果表明：围载工况下,角桩的轴力、侧摩阻力最大,边桩次之,中心桩最小;中性点位置角桩最深,边桩略高,中心桩距桩顶最近。边载工况下,靠近边载侧和中间一排桩基轴力、侧摩阻力与围载时的变化规律类似,远离边载侧的一排桩基受边载影响较小,无负摩阻力;各桩基中性点位置变化规律类似于围载工况。与围载工况相比,边载时同一位置桩身轴力、负摩阻力均较小,中性点位置较高。与单侧边载工况相比,围载时各基桩承载力安全系数FS均较小且随荷载的增大衰减梯度较大。该研究成果为不同堆载形式下群桩基础的设计提供参考。  相似文献   

Calculation and analysis of negative skin of monopile applied for offshore wind turbine     

Lu Ma 《Marine Georesources & Geotechnology》2017,35(2):275-280

Monopiles are considered to be as a kind of viable foundation types for offshore wind turbines. The effect of negative skin friction on pile foundation is always an important problem. There are very important theoretical and practical significance to study the distribution law of negative skin friction and the calculation method. Based on the special stratum, the stress and strain of the monopile and soil are simplified, and the improved Kezdi’s double-broken-line model is adopted. The analytical solution of negative skin friction of monopile is deduced according to the degree of skin friction. An engineering case was analyzed by the method, and the calculated results agree well with the measured data. The calculation method proposed can accurately describe the range of the monopile skin frictional distribution and the position of the neutral point, and it is simple and convenient to calculate, that is also a feasible method for calculating the negative skin friction of monopile of offshore wind turbines in practical engineering.  相似文献   

接地导线源电磁场全域有效趋肤深度   总被引：3，自引：2，他引：1       下载免费PDF全文

陈卫营  薛国强 《地球物理学报》2014,57(7):2314-2320

为了更好地推进广域电磁法的发展和应用,本文以接地长导线源为例,研究了可控源电磁场全场域的有效趋肤深度.利用频域电偶极源在均匀半空间产生电磁场的闭合表达式,计算了不同电磁场分量定义的有效趋肤深度,并讨论了在不同频率、不同偏移距、不同电导率情况下,有效趋肤深度的变化特性.根据有效趋肤深度随偏移距的变化特征以及与平面波趋肤深度之间的关系,利用多项式拟合的办法在五个不同频率范围内给出了适用于全场域的有效趋肤深度快速估算公式.研究结果表明：不同分量定义的有效趋肤深度是不同的,但是它们随偏移距、频率、电导率等参数的变化趋势是类似的,而且在一定范围内都趋近于平面波趋肤深度.理论模型的研究表明,有效趋肤深度可以作为测量参数选取和数据解释工作的参考依据.  相似文献   

Field Study on the Behavior of Destructive and Non-Destructive Piles Under Compression     

Qian-qing Zhang  Shu-Cai Li  Li-Ping Li 《Marine Georesources & Geotechnology》2013,31(1):18-37

This paper presents a series of full-scale load tests on long bored piles instrumented with strain gauges along the shafts, including eight field tests of piles loaded to failure and one non-destructive pile load test. The load-displacement response, skin friction, end resistance, and the threshold of the pile-soil relative displacement for fully mobilizing skin resistance were discussed. A simple softening model was proposed to describe the degradation behavior of the skin friction along the pile-soil interface and the load-displacement relationship developed at the pile base. It is found that the shaft resistance degradation investigated in the non-destructive load test only occurs at a shallow depth, and the skin friction of deeper soil is not fully developed. However, unlike the results of the non-destructive load tests, the softening is accompanied by a reduction in skin friction and observed to be along the whole pile depth. The thresholds of pile-soil relative displacement for fully mobilizing skin resistances in different soils have been found to be in the range 0.6% to 2.4% of the pile diameter. Moreover, in practical applications, a bilinear model is assumed to be feasible in analyzing the load-settlement relationship developed at the end of non-destructive pile, whereas the load transmission curve of the soils below the pile base corresponds to a softening model in the field tests of piles loaded to failure.  相似文献   

Field and Theoretical Analysis on the Response of Destructive Pile Subjected to Tension Load     

Qian-Qing Zhang  Shu-Cai Li  Li-Ping Li 《Marine Georesources & Geotechnology》2013,31(1):12-22

Most field tests are carried out using working piles for verification purposes in China, and loading tests are terminated before achieving true pile capacity. In this work, two full-scale destructive loading tests on tension piles instrumented with strain gauges were conducted to capture true pile capacity. The load-displacement response, load transfer, and threshold of the pile-soil relative displacement for fully mobilizing skin resistance in the uplift case were discussed. It was found that the shaft resistance degradation is observed to be along the pile depth with a reduction factor of 0.905 to 0.931, and the thresholds of pile-soil relative displacement for fully mobilizing skin resistance of the tension pile in different soils are found to be in the range 0.67% to 1.34% of the pile diameter. Based on the field test results, a simple softening model was proposed to describe the degradation behavior of skin friction along the pile-soil interface. Further study was conducted to assess the influence of the threshold of pile-soil relative displacement for fully mobilizing skin friction and the reduction factor on the skin friction. As to the analysis of the response of single pile subjected to tension load, a highly effective iterative computer program was developed using the proposed skin friction softening model. Comparisons of the load-settlement response for the well-instrumented tests were given to demonstrate the effectiveness and accuracy of the proposed simple method.  相似文献