Recovery of atypical Aeromonas salmonicida from ulcerated fish from the Baltic Sea     

Wiklund  T.; Tabolina  I.; Bezgachina  T. V. 《ICES Journal of Marine Science》1999,56(2):175-179

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大黄鱼病原副溶血弧菌单克隆抗体制备及其应用   总被引：5，自引：0，他引：5  

池信才  王军  鄢庆枇  苏永全 《海洋科学》2007,31(8):1-5

用甲醛灭活副溶血弧菌(Vibrio parahaemolyticus)和溶藻弧菌(V.alginolyticus)制成免疫原免疫BALB/c小鼠,利用淋巴细胞杂交瘤技术,获得1株特异的针对副溶血弧菌的单克隆抗体,命名为D6F3H5。腹水及培养上清液抗体的ELISA效价分别为:1∶5 120和1∶1 280,该单克隆抗体与其它细菌没有明显的交叉反应。利用该单克隆抗体和兔抗副溶血弧菌多克隆抗体,建立了检测副溶血弧菌的双抗体夹心ELISA方法。该方法对副溶血弧菌的最小检出量为5×104个/mL。用双抗体夹心ELISA检测大黄鱼(Pseudosciaena crocea)样品,14尾患病大黄鱼中有11尾检出副溶血弧菌,而10尾健康大黄鱼都没有检出副溶血弧菌。由此可见,本试验制备的高特异性的抗副溶血弧菌单克隆抗体,可以用于患病大黄鱼副溶血弧菌的快速诊断。  相似文献   

不同堆载形式对群桩负摩阻力的影响     

马学宁  付江  王军  王旭 《岩土力学》2018,39(10):3531-3538

在既有桥墩桩基础周围土体堆载时,会引起土体的沉降和侧向变形,进而在桩身产生负摩阻力,对桩基变形及承载性能有很大影响。为研究围载和单侧边载作用下群桩中不同位置桩基的受力差异,以3×3群桩基础为研究对象,进行围载和单侧边载作用下的模型试验,分析了不同位置桩基轴力、侧摩阻力、中性点位置和基桩承载力安全系数等的变化规律及差异。研究结果表明：围载工况下,角桩的轴力、侧摩阻力最大,边桩次之,中心桩最小;中性点位置角桩最深,边桩略高,中心桩距桩顶最近。边载工况下,靠近边载侧和中间一排桩基轴力、侧摩阻力与围载时的变化规律类似,远离边载侧的一排桩基受边载影响较小,无负摩阻力;各桩基中性点位置变化规律类似于围载工况。与围载工况相比,边载时同一位置桩身轴力、负摩阻力均较小,中性点位置较高。与单侧边载工况相比,围载时各基桩承载力安全系数FS均较小且随荷载的增大衰减梯度较大。该研究成果为不同堆载形式下群桩基础的设计提供参考。  相似文献   

Calculation and analysis of negative skin of monopile applied for offshore wind turbine     

Lu Ma 《Marine Georesources & Geotechnology》2017,35(2):275-280

Monopiles are considered to be as a kind of viable foundation types for offshore wind turbines. The effect of negative skin friction on pile foundation is always an important problem. There are very important theoretical and practical significance to study the distribution law of negative skin friction and the calculation method. Based on the special stratum, the stress and strain of the monopile and soil are simplified, and the improved Kezdi’s double-broken-line model is adopted. The analytical solution of negative skin friction of monopile is deduced according to the degree of skin friction. An engineering case was analyzed by the method, and the calculated results agree well with the measured data. The calculation method proposed can accurately describe the range of the monopile skin frictional distribution and the position of the neutral point, and it is simple and convenient to calculate, that is also a feasible method for calculating the negative skin friction of monopile of offshore wind turbines in practical engineering.  相似文献   

接地导线源电磁场全域有效趋肤深度   总被引：3，自引：2，他引：1       下载免费PDF全文

陈卫营  薛国强 《地球物理学报》2014,57(7):2314-2320

为了更好地推进广域电磁法的发展和应用,本文以接地长导线源为例,研究了可控源电磁场全场域的有效趋肤深度.利用频域电偶极源在均匀半空间产生电磁场的闭合表达式,计算了不同电磁场分量定义的有效趋肤深度,并讨论了在不同频率、不同偏移距、不同电导率情况下,有效趋肤深度的变化特性.根据有效趋肤深度随偏移距的变化特征以及与平面波趋肤深度之间的关系,利用多项式拟合的办法在五个不同频率范围内给出了适用于全场域的有效趋肤深度快速估算公式.研究结果表明：不同分量定义的有效趋肤深度是不同的,但是它们随偏移距、频率、电导率等参数的变化趋势是类似的,而且在一定范围内都趋近于平面波趋肤深度.理论模型的研究表明,有效趋肤深度可以作为测量参数选取和数据解释工作的参考依据.  相似文献   

Field Study on the Behavior of Destructive and Non-Destructive Piles Under Compression     

Qian-qing Zhang  Shu-Cai Li  Li-Ping Li 《Marine Georesources & Geotechnology》2013,31(1):18-37

This paper presents a series of full-scale load tests on long bored piles instrumented with strain gauges along the shafts, including eight field tests of piles loaded to failure and one non-destructive pile load test. The load-displacement response, skin friction, end resistance, and the threshold of the pile-soil relative displacement for fully mobilizing skin resistance were discussed. A simple softening model was proposed to describe the degradation behavior of the skin friction along the pile-soil interface and the load-displacement relationship developed at the pile base. It is found that the shaft resistance degradation investigated in the non-destructive load test only occurs at a shallow depth, and the skin friction of deeper soil is not fully developed. However, unlike the results of the non-destructive load tests, the softening is accompanied by a reduction in skin friction and observed to be along the whole pile depth. The thresholds of pile-soil relative displacement for fully mobilizing skin resistances in different soils have been found to be in the range 0.6% to 2.4% of the pile diameter. Moreover, in practical applications, a bilinear model is assumed to be feasible in analyzing the load-settlement relationship developed at the end of non-destructive pile, whereas the load transmission curve of the soils below the pile base corresponds to a softening model in the field tests of piles loaded to failure.  相似文献   

Field and Theoretical Analysis on the Response of Destructive Pile Subjected to Tension Load     

Qian-Qing Zhang  Shu-Cai Li  Li-Ping Li 《Marine Georesources & Geotechnology》2013,31(1):12-22

Most field tests are carried out using working piles for verification purposes in China, and loading tests are terminated before achieving true pile capacity. In this work, two full-scale destructive loading tests on tension piles instrumented with strain gauges were conducted to capture true pile capacity. The load-displacement response, load transfer, and threshold of the pile-soil relative displacement for fully mobilizing skin resistance in the uplift case were discussed. It was found that the shaft resistance degradation is observed to be along the pile depth with a reduction factor of 0.905 to 0.931, and the thresholds of pile-soil relative displacement for fully mobilizing skin resistance of the tension pile in different soils are found to be in the range 0.67% to 1.34% of the pile diameter. Based on the field test results, a simple softening model was proposed to describe the degradation behavior of skin friction along the pile-soil interface. Further study was conducted to assess the influence of the threshold of pile-soil relative displacement for fully mobilizing skin friction and the reduction factor on the skin friction. As to the analysis of the response of single pile subjected to tension load, a highly effective iterative computer program was developed using the proposed skin friction softening model. Comparisons of the load-settlement response for the well-instrumented tests were given to demonstrate the effectiveness and accuracy of the proposed simple method.  相似文献   

刺参(Apostichopus japonicus)腐皮综合症发生相关蛋白的分离与鉴定   总被引：1，自引：0，他引：1  

张鹏  李成华  李晔  苏秀榕  金春华  李太武 《海洋与湖沼》2013,44(3):741-746

采用比较蛋白质组学的方法,对2011年10月20日取自辽宁省普兰店室内养殖厂腐皮综合症刺参和健康个体肠组织中差异表达蛋白进行了研究。结果表明,在优化的电泳条件(80μg脱盐蛋白、pH4—7[NL,13cm]的IPG预制胶条)下,共检测到约700个蛋白质点;表达量差异两倍以上的蛋白点51个,最大表达差异比率为4.32;对其中重复性一致的30个差异点进行质谱鉴定,成功鉴定了23个差异蛋白,包括铁蛋白,过氧化氢酶,泛素相关修饰子3,细胞色素氧化酶和肌动蛋白等;进一步扫描实验室完成的刺参转录组数据,筛选了过氧化氢酶和泛素相关修饰子3的EST序列。本研究是蛋白层面上刺参腐皮综合症发生机制的有效尝试,研究结果为进一步认识该疾病的发生机制提供了参考。  相似文献   

桩基内力长期监测中的蠕变影响分析     

刘争宏  郑建国 《岩土力学》2013,34(5):1391-1396

以黄土地区桩基浸水试验为代表的桩身内力长期监测过程中,桩身混凝土除发生弹性应变外还会发生蠕变,以往工作中往往忽略了混凝土在轴力长期作用下蠕变对测试结果的影响,但蠕变真实存在,它对内力测试结果的影响程度以及如何消除其影响值得研究。根据现场实测试验资料对比分析表明,长时间的桩基内力测试应考虑桩身蠕变的影响;基于混凝土蠕变的相关理论,推导了从实测应变中扣除桩身蠕变的公式;从消除蠕变影响角度分析了黄土桩基浸水试验中应采取的措施,其关键技术是控制试验桩的混凝土强度等级,在桩顶附近设置标定段,并保持标定段混凝土的温度和湿度恒定,且与下部桩体一致,获得具有代表性的蠕变度函数曲线。  相似文献   

现浇X形桩桩承式加筋路堤三维有限元分析     

陈力恺  孔纲强  刘汉龙  金辉 《岩土力学》2013,34(Z2):428-432

桩承式加筋路堤在高速公路软基处理中得到了广泛应用,但目前对其承载变形机制还缺乏深刻认识。结合南京长江第四大桥北接线段软基加固工程,基于数值方法对现浇X形桩桩承式加筋路堤中桩土沉降、桩土应力、桩身轴力及超静孔隙水压力的发展变化规律进行了分析。研究结果表明,受土拱效应影响,路堤填筑荷载主要由桩体承担,桩及桩间土土压力在路堤填筑过程中均逐渐增大,在软基固结过程中,桩顶土压力继续增大而桩间土土压力逐渐减小,最终趋于各自的稳定值;路堤填筑过程中桩身轴力增长较快,现浇X形桩桩身上部较大部分区段存在负摩阻力,桩身中性点位置经历了先逐渐上移、而后向下移动,最终趋于稳定的过程。  相似文献