Histological Study on the Skin of Japanese Flounder Paralichthys olivaceus     

ZHU Jie  ZHANG Xiumei  and GAO Tianxiang Fisheries College  Ocean University of China  Qingdao  P.R.China 《中国海洋大学学报(英文版)》2005,4(2)

Histological development of Japanese flounder Paralichthys olivaceus larval skin and ultrastructural difference of skin between reared normal and malpigmented Japanese flounder were studied with light microscopy (LM) and transmission electron microscopy (TEM). The results show that the skin develops slowly before the metamorphosis, while at the onset of metamorphosis, the skin develops quickly and becomes complete in structure till about 50d after being hatched. Ultrastructural observation on the normal and malpigmented skins shows that the iridophore and melanophore are adjacent to each other. Profile and structure of the two kinds of pigment cells are more complete in the skin of normal ocular side than in the skin of pigmented blind side. The ultrastructure of typical chloride cell was observed in the skin of Japanese flounder larvae for the first time.  相似文献   

几种鱼皮胶原蛋白的理化特性及其影响因素   总被引：13，自引：0，他引：13  

曾名勇  张联英  刘尊英  董士远  李八方 《中国海洋大学学报(自然科学版)》2005,35(4):608-612

利用水产加工废弃物研究了鳙鱼、鲈鱼、鲫鱼鱼皮胶原蛋白的氨基酸组成、粘度、凝胶强度、热变性温度(Td)等理化特性及其影响因素。结果表明,在0．1mol／L的乙酸溶液中鱼皮胶原蛋白溶液的粘度随着胶原蛋白浓度的增大而增大,随着温度的升高而下降。热水法提取鳙鱼、鲈鱼、鲫鱼鱼皮胶原蛋白的热变性温度分别为30℃,25℃,27℃。鱼皮胶原蛋白的凝胶强度则随着粘度、温度、pH值、外加介质等的变化而变化。  相似文献   

副溶血弧菌特异性卵黄抗体(AHPND-VpIgY)对凡纳滨对虾幼体被动免疫和育苗成活率的影响     

闫茂仓  王瑶华  胡利华  张炯明  刘亚南  唐明  罗奎  黄贤克  周前进  陈炯 《海洋与湖沼》2019,50(2):443-448

将抗高致病性副溶血弧菌(Vibrioparahaemolyticus)特异性卵黄抗体(AHPND-VpIgY)应用于育苗生产,通过测定氨氮、亚硝酸盐、细菌总数、弧菌总数、相对保护率和育苗成活率,评价了AHPND-VpIgY在育苗期对凡纳滨对虾(Litopenaeusvannamei)幼体的保护效果。结果表明,0.1g/m3和0.2g/m3AHPND-VpIgY组的相对保护率为35.7%和57.2%,两者均显著高于非特异性卵黄抗体组(P0.05);两组的育苗成活率为50.8%和57.2%,比对照组分别提高19.1%和25.6%。各实验组氨氮、亚硝酸盐、细菌总数及弧菌总数与对照组无显著差异,对水质无影响。综上,特异性的AHPND-VpIgY能在不改变水质的前提下,有效提高凡纳滨对虾幼体的存活率、相对保护率和育苗成活率,从而提高凡纳滨对虾苗种产量和质量。  相似文献   

Semi-automated bathymetric spectral decomposition delineates the impact of mass wasting on the morphological evolution of the continental slope,offshore Israel     

Omri Gadol  Gideon Tibor  Uri ten Brink  John K. Hall  Gavrielle Groves-Gidney  Gideon Bar-Am  Christian Hübscher  Yizhaq Makovsky 《Basin Research》2020,32(5):1156-1183

Understanding continental-slope morphological evolution is essential for predicting basin deposition. However, separating the imprints and chronology of different seafloor shaping processes is difficult. This study explores the utility of bathymetric spectral decomposition for separating and characterizing the variety of interleaved seafloor imprints of mass wasting, and clarifying their role in the morphological evolution of the southeastern Mediterranean Sea passive-margin slope. Bathymetric spectral decomposition, integrated with interpretation of seismic profiles, highlights the long-term shape of the slope and separates the observed mass transport elements into several genetic groups: (1) a series of ~25 km wide, now-buried slide scars and lobes; (2) slope-parallel bathymetric scarps representing shallow faults; (3) slope-perpendicular, open slope slide scars; (4) bathymetric roughness representing debris lobes; (5) slope-confined gullies. Our results provide a multi-scale view of the interplay between sediment transport, mass transport and shallow faulting in the evolution of the slope morphology. The base of the slope and focused disturbances are controlled by ~1 km deep salt retreat, and mimic the Messinian base of slope. The top of the open-slope is delimited by faults, accommodating internal collapse of the margin. The now-buried slides were slope-confined and presumably cohesive, and mostly nucleated along the upper-slope faults. Sediment accumulations, infilling the now-buried scars, generated more recent open-slope slides. These latter slides transported ~10 km³ of sediments, depositing a significant fraction (~3 m in average) of the sediments along the base of the studied slope during the past < 50 ka. South to north decrease in the volume of the open-slope slides highlight their role in counterbalancing the northwards diminishing sediment supply and helping to maintain a long-term steady-state bathymetric profile. The latest phase slope-confined gullies were presumably created by channelling of bottom currents into slide-scar depressions, possibly establishing incipient canyon headword erosion.  相似文献   

16S rRNA gene phylogenesis of culturable predominant bacteria from diseased Apostichopus japonicus (Holothuroidea, Echinodermata)     

Haiyan Ma  Guoliang Jiang  Zhiqiang Wu  Xin Wang 《中国海洋大学学报(英文版)》2009,8(2):166-170

Cultured Apostichopus japonicus in China suffers from a kind of skin ulceration disease that has caused severe economic loss in recent years. The disease, pathogens of which are supposed to be bacteria by most researchers, is highly infectious and can often cause all individuals in the same culture pool to die in a very short time. The 16S rRNA gene phylogenesis of the culturable bacteria from the lesions of diseased individuals was conducted to study the biodiversity of the bacterial communities in the lesions and to identify probable pathogen(s) associated with this kind of disease. S. japonica samples were selected from a hatchery located in the eastern part of Qingdao, China. Bacterial universal primers GM5F and DS907R were used to amplify the 16S rRNA gene of bacteria colonies, and touchdown PCR was performed to amplify the target sequences. The results suggest that γ- proteobacteria (Alteromonadales and Vibrionales) of CFB group, many strains of which have been also determined as pathogens in other marine species, are the predominant bacterial genera of the diseased Apostichopus japonicus individuals.  相似文献   

16S rRNA Gene Phylogenesis of Culturable Predominant Bacteria from Diseased Apostichopus japonicus(Holothuroidea,Echinodermata)     

MA Haiyan  JIANG Guoliang  WU Zhiqiang  WANG Xin 《中国海洋大学学报(英文版)》2009,8(2)

Cultured Apostichopusjaponicus in China suffers from a kind of skin ulceration disease that has caused severe economic loss in recent years. The disease, pathogens of which are supposed to be bacteria by most researchers, is highly infectious and can often cause all individuals in the same culture pool to die in a very short time. The 16S rRNA gene phylogenesis of the culturable bacteria from the lesions of diseased individuals was conducted to study the biodiversity of the bacterial communities in the lesions and to identify probable pathogen(s) associated with this kind of disease. S. japonica samples were selected from a hatchery located in the eastern part of Qingdao, China. Bacterial universal primers GM5F and DS907R were used to amplify the 16S rRNA gene of bacteria colonies, and touchdown PCR was performed to amplify the target sequences. The results suggest that γ- proteobacteria (Alteromonadales and Vibrionales) of CFB group, many strains of which have been also determined as pathogens in other marine species, are the predominant bacterial genera of the diseased Apostichopusjaponicus individuals.  相似文献   

青岛近海表皮温度和表层温度之差的观测及模糊数学分析   总被引：2，自引：0，他引：2  

杜勇  吕红民  董申甫  凌铁军 《海洋与湖沼》1999,30(1):81-87

１９９３．１１．２３．９：００－１１．２８．９：００随“东方红”号海洋考察船,采用“走航式海面遥感参数自动观测系统”在青岛近海进行了５个昼夜的连续观测。根据所得资料,首先对表皮水曙和表层水温之差与各因子之间的关系进行了分析,然后利用数学方法对所获数据进行了计算。结果表明,海洋表皮水温与表层水温之差和海面风速,气温与水温之差及太阳辐射等均有明显关系。但此关系难以用传统数学方法准确表述。应用模糊推理方  相似文献   

船舶碰撞中空夹层钢管混凝土导管架平台损伤分析     

商文翰  张纪刚  吴书义  刘菲菲  陈文礼  赵阳 《海洋工程》2023,41(2):22-30

针对船舶与中空夹层钢管混凝土（CFDST）导管架平台碰撞问题,利用ABAQUS/Explicit非线性有限元软件建立中空夹层钢管混凝土导管腿导管架数值模型,对船舶碰撞进行数值模拟,对比分析1 m/s、2 m/s和3 m/s的3种碰撞速度下工况下导管架抗碰撞性能、耗能及损伤。从碰撞力、碰撞区域变形以及导管架能量耗散等方面详细研究中空夹层钢管混凝土导管腿作用下导管架整体的损伤模式和动力响应。结果表明：CFDST导管架在船舶碰撞荷载作用下主要由CFDST导管腿耗能,有效控制了结构整体位移变形,提升了整体刚度,有效发挥平台各构件之间组合耗能,结构抗碰撞能力得到明显提升。  相似文献   

兔抗对虾白斑症病毒(WSSV)独特型抗体的制备及其特性分析     

韦秀梅  绳秀珍  唐小千  邢婧  战文斌 《海洋与湖沼》2010,41(2):286-292

以前期已制备的抗WSSV囊膜蛋白单克隆抗体4G9(Ab1)杂交瘤细胞生产小鼠腹水,腹水经辛酸-硫酸铵法、ProteinG亲和层析法纯化后,用以制备兔抗血清,所得血清经纯化得到粗提的兔抗WSSV独特型抗体(Ab2)。采用竞争酶联免疫吸附实验、间接免疫荧光法、斑点免疫印迹和蛋白免疫印迹等实验方法分析了Ab2特性,结果表明:Ab2能识别Ab1并与WSSV竞争Ab1的抗原结合位点,是具有模拟WSSV特性的抗独特型抗体;Ab2能与中国对虾血细胞结合,并能部分阻断WSSV与血细胞膜的结合;其与中国对虾血细胞膜上结合的蛋白分子量分别为94.5、51.5和27.0kDa,由此推断,这三个蛋白为WSSV在中国对虾血细胞膜上的结合蛋白。本研究结果可为进一步研究WSSV侵染机理提供资料。  相似文献   

条斑紫菜锰超氧化物歧化酶原核表达及多克隆抗体制备   总被引：1，自引：0，他引：1  

邵林  茅云翔  阎斌伦  孔凡娜  王健 《中国海洋大学学报(自然科学版)》2010,40(4)

研究紫菜抗逆的分子机制,将本实验室克隆的条斑紫菜(Porphyra yezoensisUeda)锰超氧化物歧化酶(manganese superoxide dismutase,Mn SOD)基因全长cDNA克隆到质粒pET-30c(+)中,构建原核表达载体pET-S,转化大肠杆菌BL21(DE3)表达Mn SOD,表达产物的分子量约为30 KDa。利用不同浓度的Mn2+诱导重组Mn SOD的表达,可以检测到比活力随Mn2+浓度增高而上升的趋势。用重组Mn SOD免疫新西兰大白兔,制备了多克隆抗体,多克隆抗体的效价为1∶8 000。免疫印迹实验(Western Blot)表明该多克隆抗体具有很好的特异性。  相似文献