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孔雀石绿及其代谢物无色孔雀石绿在鲫鱼肌肉中的代谢规律 总被引：12，自引：2，他引：12
研究了孔雀石绿及其主要代谢物无色孔雀石绿在鲫鱼肌肉组织内的残留消除趋势。对鲫鱼以200 ng/mL浓度孔雀石绿进行药浴1 h,取肌肉采用高效液相色谱法测定孔雀石绿及代谢物无色孔雀石绿的残留状况。结果表明,孔雀石绿代谢相对较快。代谢产物无色孔雀石绿残留时间较长,可以作为检测的标志物。 相似文献
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通过压力一电换能器记录了温度、酸碱度和Ｃｕ＾２＋对鲫鱼或鱼呼吸作用的影响。温度变化主要影响鱼的鳃盖运动，而酸碱度和Ｃｕ＾２＋主要对鱼的咳嗽运动产生影响，这是因为咳嗽运动的作用是洗去积存于鳃上的污物。 相似文献
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牛磺酸对鲫鱼(Carassius auratus)生长、肠道细胞增殖及蛋白消化吸收相关基因表达的影响 总被引：1，自引：1，他引：0
本文探讨牛磺酸在鲫鱼生长、肠道细胞增殖分化以及蛋白质消化吸收相关基因的表达等方 面的作用。试验以480 尾[初始体质量(29.07±0.19)g]鲫鱼(Carassius auratus)为研究对象, 随机分成6 组, 每组设定4 个重复, 每个重复有鱼20 尾。鲫鱼基础饲料中添加的牛磺酸的剂量分别是0.0、1.0、 2.5、4.0、6.0 以及9.0 g/kg, 以0.0 g/kg 添加组为对照组, 试验持续8 周。结果表明: (1) 牛磺酸对鲫 鱼有促生长作用, 6.0 g/kg 添加组的鱼的增重率, 特定生长率, 蛋白质效率, 饲料系数等都显著高于 对照组(P<0.05).(2) 牛磺酸能促进肠绒毛的生长, 降低隐窝深度。(3) 牛磺酸提高了鲫鱼血清中T3、 T4 的含量, 添加6.0 g/kg 时显著高于对照组, 且此时血清尿素氮浓度显著低于对照组和其它处理组 (P<0.05).(4) 氨肽酶N(APN)、小肽转运蛋白(PepT1)、基因系尾型同源盒基因(CDX2)、L-氨基酸转 运载体蛋白(LAT2)等基因的相对表达量随牛磺酸剂量增加而上调, 6.0 g/kg 时显著高于对照组和其它 实验组(P<0.05).由此可见, 适量添加牛磺酸能影响鲫鱼生长、肠道细胞增殖及蛋白消化吸收相关基 因的表达。 相似文献
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五种鲤科鱼类生长激素cDNA的克隆和序列分析 总被引：1，自引：0，他引：1
通过RT-PCR方法,以草鱼、鳙鱼、鲫鱼、鲤鱼、齐口裂腹鱼等5种鲤科重要经济鱼类的垂体总RNA为模板扩增出其生长激素(fish growth hormone,fGH)的完整ORF序列,并克隆到pMD18-T载体上,命名为pMD-1(草鱼)、pMD-2(鳙鱼)、pMD-3(鲫鱼)、pMD-4(鲤鱼)、pMD-5(齐口裂腹鱼)。测序结果显示,ORF序列其长度均为633 bp。序列分析表明,所有ORF序列均以ATG为起始密码,以TAG为终止密码,编码210个氨基酸残基,推导的生长激素前体由22个氨基酸的信号肽和188个氨基酸的成熟肽组成。同源性分析表明,草鱼、鳙鱼、鲤鱼、鲫鱼和齐口裂腹鱼的fGH同源性在93.3%~99.5%之间。 相似文献
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铜对鲫鱼血清生化成分的影响 总被引：12，自引：0，他引：12
本文通过分析鲫鱼血清生化成分含量的变化，研究Ｃｕ对鲫鱼的致毒效应。试验结果表明，（１）血清Ｎａ＾＋，Ｃｌ＾－含量随Ｃｕ浓度的升高而增加，在水中Ｃｕ浓度为３．９１μｍｏｌ／Ｌ时达到最大值，而血清Ｋ＾＋不受Ｃｕ影响。（２）血清胆固醇，球蛋白含量和ＧＯＴ，ＡＬＰ酶活性均在Ｃｕ浓度为０．７８μｍｏｌ／Ｌ时达到最高值，若再增加Ｃｕ浓度，血清胆固醇，球蛋白含量有所下降，但仍高于对照组，而ＧＯＴ，ＡＬＰ酶活性却 相似文献
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In this study the effects of polybrominated diphenyl ether compounds (PBDEs), BDE-47 and BDE-99 on carassius aurats were discussed. The reproductive capacity viability and proliferation percent decreased with increasing BDE-47and BDE-99 concentrations (p<0.05), presenting a dose-dependent manner. Physiological biomarkers such as cholesterol (CHL), acetylcholinesterase (AChE), ethoxyresorufin-O-deethylase (EROD) and vitamin were studied to reveal the toxicological effects of BDE-47 and BDE-99 exposure. CHL levels generally decrease with increasing BDE-47 exposure. However, there were no significant differences between the samples and the control. As to BDE-99, the CHL level decreased as compared with the control level when the BDE-99 concentrations reached 20 μg/L (p<0.05). The degree of inhibition of the AChE increased with the increase of DE-47and BDE-99 concentrations. The EROD activity during 72 hours' exposure significantly increased to 20 μg/L for BDE-47 (p<0.05), however, EROD activity was not significantly different at different concentrations of BDE-99 (p<0.05). Carassius aurats hepatocytes reacted to the BDE-47 exposure with increasing EROD activities in the dose dependent manner. Although at lower concentrations, the induced effects of BDE-47 and BDE-99 were not significantly different, though BDE-47 showed strong induced effects on the EROD activity with increasing concentrations in carassius aurats hepatocytes. In contrast, BDE-99 did not influence EROD activity consistently. The VA level of carassius aurats was significantly different from that of the controls for BDE-99 (p<0.001), as far as the VA level was significantly lower of carassius aurats than the controls for BDE-47 (p<0.001). CHL, AChE, EROD, and VA levels in carassius aurats were differently affected by BDE-47 and BDE-99 exposures, therefore, they would be good indicators for exposure. 相似文献
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《广东海洋大学学报》2020,(2)
【目的】研究中药复方对鲫鱼(Carassius auratus)细菌性败血症的防治效果。【方法】由茵陈、地锦草、大黄、黄芩、白术、苦参、板蓝根按一定比例组成中草药复方,用索氏提取法以乙醇为溶剂提取中草药复方的总有效成分,提取物按质量分数0%、0.5%、2.0%,中药复方原料粗粉按质量分数2.0%的添加量制成药饵(分别记为0%、0.5%、2.0%药饵组,2.0%生药组),投喂人工感染温和气单胞菌(Aeromonas sobria)的鲫鱼,测定感染4 d和15 d时鲫鱼的血清转氨酶(GPT、AST)、甘油三酯(TRIG)、胆固醇(TC)、总蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)、白球比(白蛋白与球蛋白质量浓度比,A/G)等肝功能指标,尿素氮(BUN)、尿酸(UA)、肌酐(CR)等肾功能指标,心肌功能指标肌酸磷酸激酶(CPK)。【结果】2.0%药饵组在0.5×10~9 cfu/mL和1×10~9cfu/mL的温和气单胞菌攻毒感染下,药饵防治率分别为82.5%和42.5%,与0%药饵组相比,药效极显著(P <0.01)。相对于对照组(健康鱼),感染0.5×10~9 cfu/mL温和气单胞菌病鱼(0%药饵组)转氨酶、TRIG、TC、TP、ALB、GLB、A/G指标下降(P <0.05),BUN、UA、CR、CPK指标上升(P <0.05),而其他药饵组各指标均较0%药饵组改善,恢复至对照组水平。【结论】中药复方对鲫鱼细菌性败血症有一定防治作用,可改善病鱼的肝脏、肾、心肌功能的受损状态。以添加质量分数2%中草药复方提取物的药饵防治鲫鱼细菌性败血症的药效显著。 相似文献
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Inorganic lead （Pb） is one of the most common environmental pollutants. Much evidence indicates that Pb exposure could directly affect fish growth and development. In this study, we investigated the cytotoxic effects of Pb on cytoskeletal protein stability at both protein and mRNA level in crucian carp Carassius auratus. Pb（NO3）2 treatment in concentration of 100 μmol/L resulted in decreased expression of both α- and β-tubulin but γ-tubulin as assayed with SDS-PAGE, Western Blot, and ELISA. In vivo and in vitro analyses on protein expression of tubulins are consistent. The effect of Pb on mRNA expression varied among different tissues. Our results suggest that cytotoxicity of Pb at protein translation level is stronger than at mRNA expression level. 相似文献