Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( Ho ) and expected heterozygosity ( He ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.
The experimental work described in this paper was carried out in order to discover more about the effects of bedding planes on wave velocity and acoustic emission (AE) characteristics of shale. Two groups of specimens, which were collected from the Longmaxi shale outcrop in Chongqing, China and cored perpendicular and parallel to the bedding planes, were tested under uniaxial compression, and the wave velocity and AE were monitored. There were obvious differences in the acoustic characteristics of shale with different bedding plane orientations. The experimental results show that (1) the average increasing rates of P- and S-wave velocities were 39.86 and 54.41%, respectively, for the specimen with a load perpendicular to the bedding planes (Y-0). The P-wave velocity and axial strain of specimen show a marked logarithmic relationship. However, the average increasing rates of P- and S-wave velocities were 5.44 and 10.54%, respectively, for the specimen with a load parallel to the bedding planes (Y-90). The good linear relationship between P-wave velocity and axial strain before failure of specimen has been built. Generally, S-wave velocity was more sensitive to axial strain than P-wave velocity. (2) AE characteristics for Y-0 showed that a few signals → quiet period → stable increase → steep increase; for Y-90: quiet period → stable increase → sudden increase → sharp increase. The AE energy for two groups of specimens was concentrated on low and middle of amplitudes (45-80 dB), but the proportion of amplitudes (80–100 dB) and the total counts of AE for Y-0 was 1.95, 2.2 times as much as that for Y-90, respectively. The results preliminarily revealed the effect of bedding orientation on the wave velocity and AE properties of shale and may provide guidance for the improvement of acoustic logging and microseismic monitoring in the field. 相似文献
Frequency-dependent rupture behavior of subduction zone interplate megathrust faults has been observed by back-projection method in different frequency bands (from 0.05 to 5 Hz). It has been suggested that the down-dip region of the Tohoku megathrust radiated strongly at high frequencies (>10 Hz) compared with that of the up-dip region. By assuming the same source time function of each fault patch, we perform a synthetic sensitivity analysis to compare the energy received from the shallower parts (and further way from the receiver sites) with that from the deeper parts (and closer to the receiver sites) of the rupture. Our results indicate that regional on-shore recordings are probably not adequate to constrain the presence of far-off shore high frequency radiations because of the strong attenuation of this region. 相似文献
Of great importance for guiding numerical weather and climate predictions, understanding predictability of the atmosphere in the ocean − atmosphere coupled system is the first and critical step to understand predictability of the Earth system. However, previous predictability studies based on prefect model assumption usually depend on a certain model. Here we apply the predictability study with the Nonlinear Local Lyapunov Exponent and Attractor Radius to the products of multiple re-analyses and forecast models in several operational centers to realize general predictability of the atmosphere in the Earth system. We first investigated the predictability characteristics of the atmosphere in NCEP, ECMWF and UKMO coupled systems and some of their uncoupled counterparts and other uncoupled systems. Although the ECMWF Integrated Forecast System shows higher skills in geopotential height over the tropics, there is no certain model providing the most precise forecast for all variables on all levels and the multi-model ensemble not always outperforms a single model. Improved low-frequency signals from the air − sea and stratosphere − troposphere interactions that extend predictability of the atmosphere in coupled system suggests the significance of air − sea coupling and stratosphere simulation in practical forecast development, although uncertainties exist in the model representation for physical processes in air − sea interactions and upper troposphere. These inspire further exploration on predictability of ocean and stratosphere as well as sea − ice and land processes to advance our understanding of interactions of Earth system components, thus enhancing weather − climate prediction skills.
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