Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( Ho ) and expected heterozygosity ( He ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.
Journal of Oceanology and Limnology - Sparids are of considerable economic importance in marine fishery and aquaculture in China, and the species diversity of this group is considered relatively... 相似文献
We collected leiognathid fishes from the Hainan Island and Beibu Gulf between November 2007 and December 2008. Ten of these
specimens were identified as Nuchequula blochii (Valenciennes 1835), which is the first record of this species in China’s waters. These specimens are distinguished by the
combination of these characteristics: breast fully scaled, cheek naked, anteroventral profile of the lower jaw almost straight,
a conspicuous dark blotch distally on the anterior part of spinous portion of dorsal fin, a yellow blotch distally on posterior
part of spinous portion of dorsal fin, and a dark blotch on nape. This paper reviews the genus Nuchequula and describes three species that occur in China’s waters. 相似文献
We collected five specimens of threadfin bream from Beihai, Guangxi, China in March 2010. These were subsequently identified as red filament threadfin bream Nemipterus marginatus (Valenciennes, 1830), being the first record of this species from Chinese waters. N. marginatus is distinguished by the following characteristics: lower border of eye lies above a line from tip of snout to upper base of pectoral fin; mouth oblique, maxillary extending to lower anterior border of pupil; teeth in jaws in several rows, pointed; upper jaw with 3 to 5 pairs of small recurved canines; suborbital with straight lower edge and rounded posterior edge; pectoral fins extending to between level of anus and origin of anal fin; pelvic fins reaching to the first or second anal rays; caudal fin forked, upper lobe tails into a short reddish filament; dorsal fin bluish with a yellow margin distally and a broad yellow median band which subdivides posteriorly into 3 small bands. 相似文献
A new record of Pagrus caeruleostictus(Valenciennes,1830),collected from the Beibu Gulf,South China Sea in April 2013,was documented based on morphology and cytochrome oxidase I subunit(COI) gene barcoding analyses. It can be distinguished by a combination of the following characteristics: head scaled to above eyes; cheeks with 5 or 6 rows of scales; lateral line scales 51–52; 5 rows of scales above the lateral line; 4 and 6 cuspidate teeth in front of upper and lower jaws,respectively,followed by 2 rows of blunter teeth posteriorly; gill rakers on first arch 12 to 15; D XI–XII +9–11; A III +8–9; the first two dorsal spines very short,the third to fifth extended,filamentous in the young; the first pelvic ray filamentous; silvery pink with dark blue spots on back and sides; caudal fin pinkish; other fins bluish or pinkish; the blue spots generally disappear in large specimens. The present report of P. caeruleostictus suggests that its distribution in Chinese coastal waters may be attributed to human effort,since this species is unlikely to have expanded naturally from the eastern Atlantic to the South China Sea,with no other records from the Indian or Pacific Oceans. We recommend that a precautionary approach should be adopted for the management of P. caeruleostictus. 相似文献
The mudskipper Boleophthalmus pectinirostris and Chinese black sleeper Bostrichthys sinensis occupy the intertidal zone. However, both species have their own unique diet. The former is an herbivore and the latter is a carnivore. In order to reveal the relationship between digestive enzyme activities and diets in the two species, the activities of protease (P), non-specific bile salt-activated lipase (BAL) and α-amylase (A) were determined in the stomach and intestine of adult mudskipper B. pectinirostris and Chinese black sleeper B. sinensis. The results showed that the activities of protease, BAL and α-amylase in the intestine of B. pectinirostris were significantly (P<0.05) higher than those in the stomach. In B. sinensis, gastric protease activity was not different from the intestinal protease (P>0.05), while BAL and α-amylase activities of the intestine were significantly (P<0.05) higher than those of the stomach. The activity of gastric protease in B. sinensis was significantly (P<0.05) higher than that in B. pectinirostris, while the activities of intestinal protease were not different between the two fish species (P>0.05). BAL activities of the stomach and intestine in B. sinensis were significantly (P<0.05) higher than those in B. pectinirostris, while α-amylase activities of the stomach and intestine in B. pectinirostris were significantly (P<0.05) higher than those in B. sinensis. The ratios of P/BAL, A/P and A/BAL of the digestive tract in B. pectinirostris were 1.5, 107.3 and 158.6, respectively; and those in B. sinensis were 0.2, 1.6 and 0.2, respectively. It can be concluded that food digestion in the adult B. pectinirostris is mainly carried out in the intestine, whereas in the adult B. sinensis it is initiated in the stomach and finishes in the intestine. The activities of BAL and α-amylase in B. pectinirostris and B. sinensis are well correlated with their diets. However, a clear-cut correlation between protease activity and diets is not found in these two species. 相似文献