Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( Ho ) and expected heterozygosity ( He ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.
Of great importance for guiding numerical weather and climate predictions, understanding predictability of the atmosphere in the ocean − atmosphere coupled system is the first and critical step to understand predictability of the Earth system. However, previous predictability studies based on prefect model assumption usually depend on a certain model. Here we apply the predictability study with the Nonlinear Local Lyapunov Exponent and Attractor Radius to the products of multiple re-analyses and forecast models in several operational centers to realize general predictability of the atmosphere in the Earth system. We first investigated the predictability characteristics of the atmosphere in NCEP, ECMWF and UKMO coupled systems and some of their uncoupled counterparts and other uncoupled systems. Although the ECMWF Integrated Forecast System shows higher skills in geopotential height over the tropics, there is no certain model providing the most precise forecast for all variables on all levels and the multi-model ensemble not always outperforms a single model. Improved low-frequency signals from the air − sea and stratosphere − troposphere interactions that extend predictability of the atmosphere in coupled system suggests the significance of air − sea coupling and stratosphere simulation in practical forecast development, although uncertainties exist in the model representation for physical processes in air − sea interactions and upper troposphere. These inspire further exploration on predictability of ocean and stratosphere as well as sea − ice and land processes to advance our understanding of interactions of Earth system components, thus enhancing weather − climate prediction skills.
