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21.
采用组织化学、免疫组化和生物化学方法对栉孔扇贝外套膜中的一氧化氮合酶(EC1.14.13.39,NOS)活性进行了研究。组织化学显示结果表明,帆状部上皮细胞呈NOS强阳性,血细胞和神经纤维呈阳性;外套触手内有较多近圆形细胞和大量波浪形神经纤维呈NOS强阳性;边缘膜内有大量神经细胞和神经纤维分别呈NOS强阳性和阳性,外套环走肌束附近和血管内皮周围均有大量近圆形的强阳性细胞分布;中央膜有大量近圆形阳性细胞聚集成团块或分散分布。免疫组化显示表明,血细胞、帆状部和边缘膜上皮细胞呈神经型NOS(nNOS)和内皮型NOS(eNOS)弱阳性,而呈诱导型NOS(iNOS)阳性。生化测定结果表明,总NOS(tNOS)活力和一氧化氮(NO)含量均为中央膜最高,边缘膜次之,外套触手较低,帆状部最低,其中结构型NOS(cNOS)活力也是帆状部的最低,而iNOS活力则是外套触手的最低。栉孔扇贝外套膜可能是其神经系统中NOS的发生中心,NO-NOS体系可在其免疫防御和调节等方面发挥重要的作用。 相似文献
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Immunostimulants may improve disease resistance of aquaculture animals by promoting the nonspecific immunity response of the
organisms. Five types of saccharides, including chitosan, yeast polysaccharide, burdock oligosaccharide, seaweed polysaccharide
and lentinus edodes polysaccharide, were screened for potential use as immunostimulants by using spectrophotometry. The saccharides
were injected into Apostichopus japonicus, a sea cucumber, and the lysozyme and superoxide dismutase (SOD) activities of the coelomic fluid and epidermal slime were
monitored in six consecutive days. The results show that the lysozyme activity of the animal’s coelomic fluid was significantly
stimulated on day 2, day 4 and day 6 after the injection of the saccharides (P<0.05). The effects of chitosan and yeast polysaccharide were the most notable. The lysozyme activity of the epidermal slime
was significantly increased by chitosana, yeast polysaccharide, seaweed polysaccharide, and burdock oligosaccharide on day
1 and day 2 after the injection (P<0.05). The SOD activity of the coelomic fluid was significantly promoted by the saccharides on day 2 and day 4 post-injection
(P<0.05), while the SOD activity of the epidermal slime increased on day 2. These findings indicate that chitosan and yeast
polysaccharide are the most effective immunostimulants and potential healthy anti-disease feedstuff for A. japonicus.
Supported by the National Key Technology Research and Development Program (863 Program, No. 2006BAD09A06) and the Special
Fund of Chinese Central Government for Basic Scientific Research Operations in Commonweal Research Institutes (No. 02-2007B03) 相似文献
23.
This pathogenic study shows that the viral diseases of Chinese prawns (Penaeus chinensis. O'sbeck)is due to three kinds of viruses: epithelium envelope baculovirus of Penaeus chinensis (EEBV-PC, de-tected by the authors in 1993), infections hypodermal and hematopoietic necrosis virus, andhepatopancreatic parvo-like virus, and that the first two viruses seem to be the main pathogens of theepidemic in the northern regions in 1993. 相似文献
24.
This paper describes a rapid procedure for diagnosis of penaeid viral disease using the immune serological method. The SPA (Staphylococcal Protein A) coagglutination test in early diagnosis of penaeid viral disease has yielded satisfactory results.This is the first time SPA is used in China for diagnoses of viral diseases of marine invertebrates, especially penaeid shrimp.The SPA early diagnostic method is characterized by accuracy, rapidity, simplicity and convenience, low cost, high specificity, strong sensitivity and micro-detectability, and easy dissemination and adaptability in prawn farms. 相似文献
25.
The viral disease of penaeids is indistinct but highly dangerous because their symptoms are often masked by some secondary ones.In order to gain knowledge on how to prevent the occurrence and spread of this viral disease, diagnostic studies on the early phase of the hepatopancreatic parvo-like viral (HPV)disease of cultured Penaeus chinensis was conducted using immuno-serological techniques. The purification of HPV was successfully done by density gradient ultracentrifugation of cane sugar. 相似文献
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ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｒｅｃｅｎｔｙｅａｒｓ’ｒａｐｉｄｄｅｖｅｌｏｐｍｅｎｔｏｆｔｈｅｍａｒｉｎｅｆｉｓｈｃｕｌｔｕｒｅｉｎｄｕｓｔｒｙｉｎＣｈｉｎａｈａｓｂｒｏｕｇｈｔａｂｏｕｔｐｒｏｌｉｆｅｒａｔｉｏｎｏｆｖｉｒａｌｄｉｓｅａｓｅｓｉｎｔｈｅｉｎｄｕｓｔｒｙａｓｓｈｏｗｎｂｙｔｈｅｎｏｔａｂｌｙｆｒｅｑｕｅｎｔｏｃｃｕｒｒｅｎｃｅｏｆｄｉｓｅａｓｅｓｏｒｄｅａｔｈｓｏｆｔｈｅｍａｒｉｃｕｌｔｕｒｅｄｆｉｓｈ .Ｅｌｅｃｔｒｏｎｍｉｃｒｏｓｃｏｐｅｅｘａｍｉｎａｔｉｏｎｏｆｔｈｅａｆｆ… 相似文献
27.
中国对虾肝胰脏细小病毒（ｈｅｐａｔｏｐａｎｃｒｅａｔｉｃｐａｒｖｏｖｉｒｕｓｄｉｓｅａｓｅ,ＨＰＶＤ）是一种广泛流行于中国对虾中的危害性较大的疾病。由于该病没有特异的肉眼症状,因此早期、快速诊断方法的研究就十分重要。作者曾完成了ＳＰＡ协同凝集试验法和免疫金银染色法的诊断研究。本文用直接荧光抗体法进行研究,观察其诊断效果。结果是,将抗ＨＰＶ的荧光抗体滴加到对虾肝胰脏组织涂片以后,在某些样品的肝胰脏上皮细胞中出现了圆形、椭圆形,大小６～１３μｍ的黄绿色荧光物。经比较,该荧光物的大小和形状与同一样品病理组织切片上经Ｈ－Ｅ染色后上皮细胞中出现的ＨＰＶ包涵体是一致的。由此认为,这些存在于肝胰脏上皮细胞内的荧光物是ＨＰＶ包涵体,而直接荧光抗体法用于诊断中国对虾ＨＰＶ是有效可行的。 相似文献
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本文运用气相色谱法,对渤海微生物自然混合菌群降解20号重柴油中的烷烃的能力进行了实验室研究。 实验测定了在5,50,500ppm三个油浓度下微生物自然混合菌群对正烷烃的降解能力。20天内,微生物对正烷烃(C_(11)—C_(22))的生物降解率分别为56.63％,61.45％和72.36％;物理化学降解率分别为34.18％,38.18％和19.85％。正烷烃的总降解率分别为90.81％,99.63％和92.21％。在本实验条件下,经过20天的降解,20号重柴油中的C_(11)—C_(22)烷烃绝大部分被降解。 相似文献
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