产新琼四糖的海洋微泡菌属AG1热稳定性β-琼胶酶的过量表达与鉴定     

朱艳冰  高贺  李鹤宾  倪辉  姜泽东  李利君  肖安风 《海洋学报(英文版)》2019,38(2):96-106

从微泡菌属AG1（Microbulbifer sp. AG1）克隆得到1302 bp大小的琼胶酶基因，该基因编码产物为一个成熟蛋白（413个氨基酸残基）外加一个信号肽（20个残基）。将不含信号肽片段的琼胶酶在E. coli BL21 （DE3）中进行了异源表达和纯化。使用琼脂糖作为底物，该重组琼胶酶的最适反应温度和pH分别为60℃和7.5。该重组酶表现出优良的热稳定性，在50℃和60℃下处理1 h，重组琼胶酶仍能分别保持67%和19%的残余酶活力。除了SDS，重组琼胶酶对于其他测试的抑制剂、去垢剂和尿素变性剂有着较好的抗性。利用薄层色谱和以对硝基苯-α/β-D-吡喃半乳糖苷为底物的酶活力分析结果表明，该重组琼胶酶为β型琼胶酶，它水解琼脂糖的主要终产物为新琼四糖，而且不同聚合度的酶解产物具有抗氧化活性。  相似文献   

A Raman investigation on the boron form occurring in salt lake brine during evaporation          下载免费PDF全文

彭姣玉  陈婧  王兴鹏  董亚萍  李武 《盐湖研究》2019,27(1)

Boron resources are abundant in Da Qaidam salt lake of Qaidamu Basin. It has been given great attention for the polyborate species present in brine. In this study, the Raman spectroscopy was applied to investigate the existing-form of boron in brine during evaporation. The prepared solutions of MgO·2B2O3-H2O, MgO·2B2O3-MgCl2 -H2O, and MgO·2B2O3-MgSO4-H2O was also evaporated and recorded to study the influence of boron concentration, pH, and electrolytes on the borate speciation in brine. The mononborates of B(OH)3 and B(OH)4- were found to be the only forms present in the original salt lake brine. Brine evaporation promotes the formation of polyborate anions B3O3(OH)4-, B5O6(OH)4-, and B6O7(OH)62- and also disappearance of the B(OH)4- ion in brine with boron concentration of more than 11 g/L in B2O3. The pentaborate ion of B5O6(OH)4- was sensitive to the solution pH and found to be appeared under the pH value of 8.0. While the hexaborate ion of B6O7(OH)62- was observed more dependent on the electrolyte of magnesium chloride due to its special properties, such as promoting boron accumulation, lowering solution pH, and also the strong af?nity for water molecules, which is beneficial to the polymerization of borate ions in brine. The interaction mechanisms among polyborate anions during evaporation had also been proposed.  相似文献   

The Three American Wests     

Joe Weber 《The Professional geographer》2019,71(2):239-252

In the twenty-first century, there are three American Wests, which cut across cultural, political, physical, and economic boundaries. Parts of the West are booming, building homes, and adding population; others are legally off limits to such development; and much of the West has been bypassed by such development and growth. These are called here the Booming West, Protected West, and Bypassed West. Maps of climate, political boundaries, ethnic and racial identities, or presidential voting patterns do not match these three Wests. Each West has a different relationship to the others and might see them as threats or opportunities, and each West can be expected to grow in different areas at the expense of other Wests. The boundaries between the three Wests could be important locations; sharp transitions can exist between Wests, perhaps nowhere more so than where a fast growing metro area abuts a national park or forest boundary. Finally, the effects of climate change cut across the three Wests and have different implications for their future. Continued population growth will further emphasize the boundaries between each of the three Wests and raise the stakes for their control.  相似文献   

Corrigendum to “Pb,Cu and Cd distribution in five estuary systems of Marche,central Italy” [Mar. Pollut. Bull. 96 Issue 1–2, (15 July 2015) 441–449]     

Anna Annibaldi  Silvia Illuminati  Cristina Truzzi  Giulia Libani  Giuseppe Scarponi 《Marine pollution bulletin》2017,114(1):635

  相似文献   

Taxonomic revision of Cylindroporella ? lusitanica Ramalho, 1970: In search for the origins of the Family Dasycladaceae          下载免费PDF全文

Bruno R. C. Granier  Ana C. Azerêdo  Miguel M. Ramalho 《Island Arc》2017,26(3)

A study of new material of Cylindroporella ? lusitanica Ramalho, 1970, from the Upper Jurassic (Oxfordian) strata of Portugal identified features not observed previously. This includes the pattern of its laterals, including the gametophores. The algal body plan is unique and justifies the taxonomic reassessment of this species and the introduction of Barattoloporellopsis n. gen. Furthermore this species is most likely a primitive Dasycladaceae, a “missing link” between the modern ones (starting from the Cretaceous times) and their Jurassic ancestors.  相似文献   

The evolution of zircon during low‐P partial melting of metapelitic rocks: theoretical predictions and a case study from Mt Stafford,central Australia     

WEI‐ Wang  E. Dunkley  G. L. Clarke  N. R. Daczko 《Journal of Metamorphic Geology》2014,32(8):791-808

Palaeoproterozoic metasedimentary migmatite reflects the highest temperature parts of a regional aureole at Mt Stafford, central Australia, comprising rocks that experienced 500–800 °C at ≈3 kbar. Whole‐rock major element concentrations are correlated with Zr content, psammitic compositions having nearly twice the Zr content of pelitic compositions. Zirconium is concentrated in mesosome compared with leucosome. Zircon is largely detrital, mostly lacking any overgrowth contemporary with migmatite formation. Comparatively small proportions of micro‐zircon (<10 μm) in sub‐solidus rocks are mostly hosted by quartz and plagioclase. Much higher proportions (three to five times) of micro‐zircon in migmatite are hosted by prograde K‐feldspar, cordierite and biotite. T–X and P–T NCKFMASHTZr pseudosections constructed using thermocalc model the distribution of Zr between solid and silicate liquid phases. Half of the detrital zircon (~100 ppm Zr) is predicted to be dissolved into silicate liquid at ≈800 °C and all dissolved by 850 °C, if all zircon is involved in the equilibration volume. Melt segregation at relatively low temperature is predicted to enrich the residuum in Zr, consistent with the observed distribution of Zr between mesosome and leucosome. The limited development of metamorphic zircon rims or overgrowths at Mt Stafford is explained by three concurrent processes: (i) Zr liberated during prograde metamorphism formed micro‐zircon, rather than following the prediction that Zr will partition into silicate liquid; (ii) some detrital zircon was probably armoured by other rock‐forming minerals, reducing Zr content in the effective bulk rock composition; and (iii) small proportions of melt loss during migmatization removed Zr that otherwise would have been available to form metamorphic rims.  相似文献   

土耳其卡赞天然碱矿溶采对接井工程HSE管理          下载免费PDF全文

涂运中  李保红  隆东  刘海翔  王万  刘一枫  盛春 《探矿工程》2020,47(8):85-93

HSE管理是现代企业走出国门、承揽国际大型项目的必备条件，并且在现代工程管理中具有越来越重要的地位。土耳其卡赞天然碱矿水溶开采对接井工程的实施过程中，在借鉴国内外相关行业经验的基础上，结合项目自身特点，成立了HSE管理机构，加强了人员安全培训和机械设备的升级改造，使之符合当地的行业安全标准。引入了JSA工作安全分析工具，建立了切实可行的HSE管理体系，并强调了现场监督，从而实施了行之有效的HSE管理，为该项目的顺利运行提供了有力的安全保障。  相似文献   

Traces of volcanic ash from the Mediterranean,Iceland and North America in a Holocene record from south Wales,UK     

Gwydion Jones  Siwan M. Davies  Richard A. Staff  Neil J. Loader  Sarah J. Davies  Michael J. C. Walker 《第四纪科学杂志》2020,35(1-2):163-174

A tephra record is presented for a sediment core from Llyn Llech Owain, south Wales, spanning the early- to mid-Holocene. Seven cryptotephra deposits are discovered with three thought to correlate with known eruptions and the remaining four considered to represent previously undocumented events. One deposit is suggested to correlate with the ~6.9 cal ka bp Lairg A tephra from Iceland, whereas more distant sources are proposed as the origin for two of the tephra deposits. A peak of colourless shards in early-Holocene sediments is thought to tentatively correlate with the ~9.6 cal ka bp Fondi di Baia tephra (Campi Flegrei) and a second cryptotephra is tentatively correlated with the ~3.6 cal ka bp Aniakchak (CFE) II tephra (Alaska). The Fondi di Baia tephra has never been recorded beyond proximal sites and its discovery in south Wales significantly extends the geographical distribution of ash from this eruption. The remaining four cryptotephra deposits are yet to be correlated with known eruptions, demonstrating that our current understanding of widespread tephra deposits is incomplete. This new tephra record highlights the potential for sites at more southerly and westerly locations in northwest Europe to act as repositories for ash from several volcanic regions.  相似文献   

江南造山带(湖南段)金矿成矿规律与资源潜力   总被引：1，自引：0，他引：1  

黄建中  孙骥  周超  陆文  肖荣  郭爱民  黄革非  谭仕敏  隗含涛 《地球学报》2020,41(2):230-252

扬子地块—华夏地块经历武陵期—雪峰期增生造山-碰撞造山形成江南造山带,构成统一的华南板块,进入板内演化阶段。本文从构造-岩浆作用-沉积建造角度,结合地质年代学、古地磁、岩相古地理分析,加里东运动、印支运动属陆内作用,造就了加里东期、印支期两次主要的金矿成矿事件。湖南雪峰山—幕阜山(俗称“金腰带”)加里东期和印支晚期金矿床分区成带产出,构成一条复合型造山型金矿带。区域性构造导矿、次级构造交汇或叠加控矿明显;矿石普遍发育条带状构造,属韧性剪切递进变形的产物。通过对区域成矿背景、金矿成矿理论,金矿床(体)地质特征,结合同位素地球化学、地质找矿成果及深部验证情况等多方面研究表明该成矿带深部找矿潜力巨大,2000 m以浅金远景资源量有望达到3000 t。  相似文献   

Spatial estimation of water-table depth by artificial neural networks in light of ancillary data     

Mehrdad Pasandi  Narjes Salmani  Nozar Samani 《水文科学杂志》2017,62(12):2012-2024

Proper estimation of the spatial distribution of water-table depth is highly important in most groundwater studies. Groundwater depth is measured at specific and limited points and it is estimated for other parts using spatial estimation methods. In this study, two multivariate methods, artificial neural network (ANN) and multiple linear regression (MLR), are examined to estimate water-table depth in an unconfined aquifer located in Shibkooh, Iran. The different ancillary data, including spatial coordinates, digital elevation model (DEM), aquifer bed elevation, specific resistivity and aquifer thickness were used to improve estimates based on these methods. It was proved that performance of the ANN surpasses that of the MLR method. Using the spatial coordinates, the aquifer bed elevation and aquifer thickness resulted in the optimum spatial estimation of the water-table depth. These parameters, directly or indirectly, affect the water-table depth estimation through techniques such as ANN capable of modelling of nonlinear relationships.  相似文献