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51.
目的 研究干扰心肌组织中肉毒碱脂酰转移酶-1b（CPT1b）的表达对高脂饮食诱导的肥胖小鼠心肌细胞钙调控的影响。方法 4周龄的雄性C57小鼠,随机分为正常对照组（N-mock）、肥胖对照组（O-mock）及肥胖干预组（O-CPT1b）,采用高脂饮食诱导肥胖。6周龄时,经心肌分别注射靶向CPT1b（O-CPT1b）或靶向无关基因（N-mock、O-mock）的重组慢病毒,以下调目标基因的表达。10周后,取小鼠左心室组织检测RNA干扰的效率;采用Western blot法检测左心室组织中肌浆网钙泵（SERCA2a）的蛋白表达;并分离单个心室肌细胞,使用活细胞工作站检测心肌细胞钙瞬变。结果 肥胖小鼠心肌组织中CPT1b的表达增加,慢病毒介导的RNA干扰显著下调其表达。肥胖引起心肌细胞SERCA2a的蛋白表达降低及肌质网钙处理能力的下降,下调CPT1b的表达增加了SERCA2a的含量,并改善了钙调控异常。结论 下调心肌组织中CPT1b的表达可改善肥胖所导致的心肌细胞钙调控异常。 相似文献
52.
本研究利用线粒体16S rDNA和核基因18S rDNA片段,对泰国沿海常见的有毒水母进行遗传分析,并比较了2个基因片段作为通用分子标记,在研究水母类多个纲的遗传多样性中的应用。研究发现,泰国近海的有毒水母存在较高的遗传多样性,所获得的32个样品可以分为9个种,包括4种钵水母、4种立方水母和1种水螅水母。然而,完全确定各种的分类地位,还需要更多的形态、生活史等方面信息。两个基因片段均能明确区分各种类,但核基因18S序列比线粒体基因片段更为保守。根据16S基因片段序列计算水母种内和种间的K2P(Kimura 2-parameter)遗传距离,发现所研究的9个水母种类,种内遗传距离在0~0.050之间,其中94%的种内遗传距离小于0.040,同纲种间的遗传距离为0.204~0.474,其中91%的种间遗传距离大于0.250;而利用18S基因,种内距离在0~0.002之间,同纲种间距离为0.008~0.066(平均为0.038,SE=0.006)。16S的AT碱基含量明显高于核基因18S,且16S的碱基含量在不同纲之间有显著差异,进一步表明水母线粒体16S基因的突变率相对较高,适合研究水母较低分类阶元以及种下的遗传差异。 相似文献
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Protein and RNA in lake sediments tend to be decomposed progressively with time and sedimentation depth. Their concentrations
tend to decrease starting from the sedimentation depth of 17 cm and that of 19 cm, respectively. However, the products of
their decomposition —amino acids and nucleotides show different rules of variation. At the depth from 27 cm to 30 cm the amino
acids are most abundant in the pore waters of lake sediments. Such variation tendency seems to be related to the extent to
which microbes utilize amino acids and nucleotides. Due to polymerization in the geological processes and the adsorption of
protein on minerals and organic polymers, below the sedimentation depth of 17 cm there is still a certain amount of protein
in the sediments. With the time passing by, protein has been well preserved in various sediment layers, indicating that its
decomposition is relatively limited. The peak values of protein content in the sediments of the two lakes are produced in
the surface layers at the depth of 10 cm, implicating that the surface sediments are favorable to the release of protein.
The contents of amino acids in the pore waters of lake sediments are closely related to the activities of microbes. Below
the depth of 27 cm, the amino acids are significantly accumulated in Lake Aha sediments, probably indicating the weakening
of microbial activities.
The research project was financially supported jointly by the Ntional Natural Science Foundation of China (No. 40173038) and
the Guizhou Provincial Foundation (No. 3090) and the Chinese Academy of Sciences Knowledge-Innovation Foundation (KZCX2-105). 相似文献
55.
We develop the ANNs (Artificial Neural Networks) method to explore contaminant concentration profiles observed in soils of polluted sites. ANNs are particularly efficient in simultaneous analysis of numerous parameters and in identification of complex relations involving field data. Applying the ANN models on a PAH (Polycyclic Aromatic Hydrocarbon) database, we extracted the most characteristic components of known contaminations and applied it to identify the source type of similar polluted sites. The performed tests prove the generalisation capability of the selected ANN model. To cite this article: A. Dan et al., C. R. Geoscience 334 (2002) 957–965. 相似文献
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58.
V.P. EdgcombW. Orsi H.-W. BreinerA. Stock S. FilkerM.M. Yakimov T. Stoeck 《Deep Sea Research Part I: Oceanographic Research Papers》2011,58(10):1040-1048
The combination of nearly saturated salt concentration and corresponding high density, high hydrostatic pressure, absence of light, anoxia, and a sharp chemocline make the deep hypersaline anoxic basins in the Eastern Mediterranean Sea some of the most polyextreme habitats on Earth. Using kinetoplastid-specific primers, we detected kinetoplastid flagellates in some of the harshest deep-sea environments known to date, including some whose small subunit ribosomal RNA gene sequences are not closely related to cultured representatives. Kinetoplastids, including presumably novel representatives appear to be specialists of halocline environments in the Eastern Mediterranean, and to comprise a significant fraction of the protist communities in the brines and haloclines of several basins. Fluorescent in situ hybridization data indicate a novel ‘unidentified’ sequence clade of kinetoplastids related to bodonids represents as much as 10% of the total protist community in the Discovery Basin halocline. Different kinetoplastid groups are unevenly represented in the different basins and habitats we sampled, which we discuss as a result of environmental selection. 相似文献
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养殖大菱鲆烂鳍病病原菌的鉴定及系统发育学研究 总被引：9，自引：0，他引：9
从山东半岛3个养殖场各分离到一株大菱鲆烂鳍病的病原菌,均为革兰氏阴性,短杆状,极生鞭毛,有运动能力,菌落半透明。经常规生理生化特征测试和16SrRNA基因序列对比,表明它们都是同一种细菌。理化特征的结果显示这3株菌均与V．anguillarum的表性特征非常相似。对其16SrRNA基因序列进行分析,并构建了系统发育树,结果表明这些菌株与V．anguillarum亲缘关系最近。综合上述研究结果,将该菌鉴定为V．anguillarum. 相似文献