Nematode Diversity of Qingdao Coast Inferred from the 18S Ribosomal RNA Gene Sequence Analysis   总被引：1，自引：0，他引：1  

SHEN Xiquan YANG Guanpin LIU Yongjian 《中国海洋大学学报(英文版)》2007,6(2):132-136

The 18S ribosomal DNA gene （18S rDNA） sequences （approxtmately 1300 bp in length） were amplified from the DNA extracted from the free-living marine nematodes collected from the inter-tidal sediment of Qingdao coast in bulk with nematode specific primers. The PCR products were cloned, re-amplified, digested with Rsa I and Hin61 restriction endonucleases and separated in agarose gel. Among 17 restriction fragment length types, types 1, 2 and 6 covered 61.2%, 14.4% and 9.3% of the clones analyzed, respectively, while the remaining 14 only covered 21 clones, which accounted for 15.1% of the total. Twenty-four representative clones were sequenced and phylogenetically analyzed by referring to those currently available in RDP and GenBank databases. Although it was hard to assign these sequences to known species or genera due to the lack of the 18S rDNA sequence data of known marine free-living nematodes, the obtained sequences were assigned to the nematodes of Adenophorea. Among them, twelve sequences were close to Pontonema vulgate and Adoncholaimus sp., four to Daptonema procerus and two （identical） to Enoplus brews. Our results showed that free-living marine nematode diversities could be determined by PCR retrieving and analysis of the 18S rDNA sequences and an 18S rDNA sequence could be assigned to a species or a genus only if the 18S rDNA sequences of the free-living marine nematodes were accumulated to some extent.  相似文献   

泥蚶(Tegillarca granosa)血细胞转录组序列中免疫相关基因的结构与mRNA表达量分析     

钟爱华  储张杰  王伟洪  牟毅 《海洋与湖沼》2020,51(2):354-363

贝类血细胞直接参与异物吞噬和包囊作用,还在伤口修复、炎症反应过程中发挥重要作用,是机体免疫防御的承担者。本研究采用RNA-seq测序技术研究了鳗弧菌感染前后泥蚶血细胞的转录组序列,通过序列组装,获得了α-葡萄糖苷酶(GAA)、α-甘露糖苷酶(LAMAN)、芳基硫酸酯酶B(ASB)、天冬酰胺氨基葡萄糖酶(AGA)、整合素β3亚基(ITB3)、热休克蛋白9(HSPA9)和Toll相互作用蛋白(TOLLIP)序列,利用Orffinder获得了其编码蛋白序列,并对蛋白序列进行了理化性质、二级结构、保守结构域和三级结构分析。结果表明:7种蛋白质二级结构均含有α螺旋、β折叠及无规则卷曲,泥蚶和人类ITB3和TOLLIP具有相同的保守结构域, Swiss-model同源建模获得的三级结构与模板同源性均超过35%(TOLLIP除外)。鳗弧菌刺激后,GAA、LAMAN、ASB、AGA和HSPA9的表达量显著增加,ITB3和TOLLIP表达量变化不明显。该研究补充和完善了对泥蚶免疫相关基因的认识,并为进一步开展泥蚶抗病机理研究提供了重要的理论依据。  相似文献   

Buried scour marks as indicators of palaeo-current direction at the Mary Rose wreck site     

R. Quinn  J. M. Bull  J. K. Dix 《Marine Geology》1997,140(3-4):405-413

A 3-dimensional seismic reflection survey (using a Chirp source) of the excavated Mary Rose wreck site (King Henry VIII's flagship, wrecked in 1545) was conducted in the East Solent, off the south coast of England. The high resolution geophysical survey identified two ‘brightspot' anomalies, buried to a depth of 4–5 m, trending east–west adjacent to the western margin of the excavation hole. These anomalous reflectors are interpreted as infilled palaeo-scour features associated with the wrecking and subsequent degradation of the Mary Rose. The features were previously unrecognized on the site, and to the authors' knowledge this is the first time that such preserved longitudinal palaeo-scour marks have been recognized in the sedimentary record. Implications associated with the preservation and identification of the scour features are discussed.  相似文献   

营养盐对微囊藻细胞组分及多糖组成的影响   总被引：1，自引：1，他引：0  

代晓炫  朱伟  李明 《湖泊科学》2013,25(2):277-282

胞外多糖是微囊藻形成群体的物质基础,营养盐是否会通过影响微囊藻多糖的含量进而影响群体的形成还不清楚.在室内培养实验中,设置不同N、P浓度的培养液并测定对数期胞内的细胞组分以及3种形态的多糖含量,从而探讨N、P对细胞中多糖的影响规律.实验结果表明:RNA在细胞中的含量相对恒定(约2.0 pg/cell).N对单细胞的蛋白质和多糖都产生了显著影响:N浓度的升高显著促进蛋白质的合成,细胞中总糖的含量降低.P浓度的升高同样造成细胞中多糖总量减少,但其对蛋白质影响不大(在4.6 pg/cell左右波动).N浓度的增加还促进多糖向胞外分泌并溶解于培养基中.在高生长率下,由于细胞中RNA、蛋白质的合成速度加快,多糖的合成较慢导致胞外固着性多糖减少,这可能是室内培养难以得到群体的原因之一.  相似文献   

Detection of Prorocentrum donghaiense using sandwich hybridization integrated with nuclease protection assay   总被引：1，自引：0，他引：1  

CHEN Jie  ZHEN Yu  MI Tiezhu  YU Zhigang 《海洋学报(英文版)》2009,28(2):121-126

Prorocentrum donghaiense is an important harmful algae bloom (HAB) causing creature in China’s seas, and the conventional visual detection can not cope with long-term monitoring and high-throughput sampling projects. An assay for P. donghaiense with sandwich hybridization integrated with nuclease protection assay (NPA-SH) was established. Tests with mixed samples and spiked field ones confirmed its good specificity and sensitivity. The cell number of P. donghaiense correlated well with the optical density, ...  相似文献   

马氏珠母贝PfAQP4免疫功能初探     

潘肖兰  刘惠茹  许濛  许瀚之  张华  何毛贤 《热带海洋学报》2021,40(2):83-89

文章对马氏珠母贝水通道蛋白基因PfAQP4进行免疫功能分析。利用实时荧光定量分析免疫刺激和RNA干扰后PfAQP4及免疫相关基因的表达模式, 旨在为马氏珠母贝自然免疫研究提供新的资料。结果表明, 注射脂多糖LPS后, 在外套膜组织中PfAQP4 mRNA表达量在24h时显著上调(p<0.05), 在消化腺中PfAQP4 mRNA表达量先在12h时显著下调(p<0.05), 后在24h、36h和48h时显著上调(p<0.05)。注射聚肌胞poly(I:C)后, 在外套膜组织中PfAQP4 mRNA表达量在36h时和48h时显著上调(p<0.05), 在消化腺中PfAQP4 mRNA表达量在48h时显著上调(p<0.05)。RNA干扰PfAQP4表达后, 免疫相关基因CuZn-SOD在外套膜中的表达量显著下降(p<0.05), Pearson相关性分析显示PfAQP4与CuZn-SOD的表达存在显著正相关(r=0.818, p<0.001)。综上所述, PfAQP4参与马氏珠母贝的免疫应答。  相似文献   

Modification of the polyethylene glycol 6000 precipitation method for recovering human and indicator viruses from oysters and mussels     

Gillian D. Lewis  Angela Hough  David H. Green  John E. Hay  Lynnette R. Ferguson 《新西兰海洋与淡水研究杂志》2013,47(4):443-447

Human viruses are a common contaminant of shellfish affected by human sewage wastes. They are difficult to detect as they are not easily separated from shellfish tissue. This paper describes a modification of the polyethylene glycol (PEG) precipitation technique for recovery of enteroviruses and F‐specific bacteriophages from the Pacific oyster (Crassostrea gigas) and the green‐lipped mussel (Perna canaliculus). Modifications adopted were the use of only the digestive gland tissue for virus extraction, resuspension of the primary PEG pellet in 4 volumes of eluent, and the introduction of a secondary PEG precipitation to reconcentrate the virus containing extract. The recovery rate of the virus extraction process was not affected by introduction of the secondary concentration step (overall recovery remained at 60–70% of the virus input). The advantages of reduction of tissue residue in the extract, smaller final volume, and the ability to process 2–3 times the number of individual shellfish for the same effort, improve the practicality of the method.  相似文献   

Sea-level changes and related depositional environments on the southern Marmara shelf     

Mustafa Ergin  Nizamettin Kazanci  Baki Varol     zden Ileri  Levent Karadenizli 《Marine Geology》1997,140(3-4):391-403

Petrographic data obtained from 182 surface sediment samples together with the available bathymetric data are used to investigate the effects of the last major sea-level changes on shelf evolution in the southern Sea of Marmara. Grain-size analysis reveals the presence of at least three belts or zones which are rich in coarse-grained (sand and gravel) sediments. These coarse-grained belts which are up to 45 km long, 15 km wide, show up to 20 m of relief and are presently found at 40–80 m (average 60 m) water depths. Based on microscopic examination and residual analysis, the sediments from these belts are interpreted as indicators of high-energy shallow waters where detrital siliciclastics, with some benthic contribution, accumulated. The presence of a 62–65 m deep sill in the Çanakkale Strait and the consideration of sea-level curves would suggest that the Marmara shelves must have been subaerially exposed down to −65 m water depths for about 10,000 yrs (22,000–12,000 yrs B.P.), sufficient time to modify former shelf topographies and form such bottom relief. While difficult to date, we believe that coarse-grained belts found on the southern Marmara shelf must be relict (i.e., former shorelines, beaches) and their formation is largely related to low stands of sea-level during the Late Pleistocene regression and early Holocene transgression. However, the available high-resolution seismic profiling data suggest that the neotectonism in this seismically active Sea of Marmara plays an important role to explain the raise of these older shorelines to their present levels on the sea-floor.  相似文献   

Field measurements of fairweather bottom boundary layer processes and sediment suspension on the Louisiana inner continental shelf   总被引：1，自引：0，他引：1  

L. D. Wright  C. R. Sherwood  R. W. Sternberg 《Marine Geology》1997,140(3-4):329-345

Field measurements of bottom boundary layer and sediment-transport processes were made on the Louisiana inner continental shelf in spring 1992 at a depth of 15.5 m, and in spring and summer 1993 at a depth of 20.5 m. Two different wave–current boundary layer/sediment-transport models were applied to the measured near-bed flows. In addition, the log-profile method was applied to estimate hydraulic roughness and bed stress. Consistent with the results of others, our measurements show that near-bed flows were very weak under non-storm conditions. Bed stresses were typically too low to resuspend bed sediments. However, the advection of high-turbidity layers or plumes past the instrumentation apparently caused a sustained period of high suspended sediment concentration throughout the log layer in spring 1993. In the absence of wave activity or high suspended sediment concentrations, boundary layer profiles showed the bed to have been hydraulically very smooth with cm. However, wave agitation, combined with increased suspended sediment concentration caused hydraulically rough conditions with cm.  相似文献   

Diatom production in the marine environment: implications for larval fish growth and condition   总被引：5，自引：0，他引：5  

St. John  M. A.; Clemmesen  C.; Lund  T.; Koster  T. 《ICES Journal of Marine Science》2001,58(5):1106-1113

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