东海表层沉积物中氨氧化微生物群落的空间分布和季节性变化   总被引：2，自引：0，他引：2  

贺惠  甄毓  米铁柱  路兴岚  于志刚 《海洋学报(英文版)》2015,34(8):83-92

Ammonia oxidation plays a significant role in the nitrogen cycle in marine sediments. Seasonal and spatial distribution of ammonia-oxidizing archaea(AOA) and betaproteobacteria(β-AOB) in surface sediments from the East China Sea(ECS) were investigated using ammonia monooxygenase α subunit(amo A) gene. In order to characterize the community of AOA and β-AOB, real-time quantitative polymerase chain reaction(q PCR) was carried out in this study, along with environmental parameters. The abundance of β-AOB amo A gene(2.17×106–4.54×107 copy numbers per gram wet weight sediment) was always greater than that of AOA amo A gene(2.18×105–9.89×106 copy numbers per gram wet weight sediment) in all sampling stations. The q PCR results were correlated with environmental parameters. AOA amo A gene copy numbers in April were positively related to temperature and nitrite concentration(p0.05). β-AOB amo A gene copy numbers in August correlated negatively with salinity(p0.01), and correlated positively with ammonium concentration(p0.05). With the increase of salinity, the amo A gene copy ratio of AOB to AOA had a tendency to decrease, which suggested β-AOB dominated in the area of high level ammonium and AOA preferred high salinity area.  相似文献   

Fluid inclusions as a tool to constrain the preservation conditions of sub-seafloor cryptoendoliths     

M. Ivarsson  C. Broman  S. Lindblom  N.G. Holm 《Planetary and Space Science》2009,57(4):477-490

The combination of fluid inclusion analyses and microfossil analyses is an excellent method to study the preservation process of deep sub-seafloor microorganisms. By studying fluid inclusions in the same mineral phases as microfossils, it is possible to reconstruct the conditions that prevailed when the microorganisms where entombed and to put them in a geological and environmental context.This study has been performed on carbonate and gypsum veins in drilled basalt samples from three seamounts belonging to the Emperor Seamounts in the Pacific Ocean: Detroit, Nintoku and Koko Seamounts. The study show that variations in salt composition (MgCl₂, NaCl, KCl and CaCl₂) and salinity (2.1 and 10.5 eq. wt% NaCl) of the hydrothermal fluids do not have an influence on the occurrence of microfossils throughout the samples. The microorganisms were trapped and entombed at minimum temperatures of ∼130 °C which implies that the microorganisms could have existed at temperatures of ∼130 °C for shorter periods of time. The microorganisms were entrapped at shallow-marine to submarine conditions and the entrapment of the microorganisms occurred relatively late compared to the volcanic activity.  相似文献   

Bioremediation of sulphate rich mine effluents using grass cuttings and rumen fluid microorganisms   总被引：1，自引：0，他引：1  

Harma A. Greben  Jacobin Baloyi  Julia Sigama  Stephanus N. Venter 《Journal of Geochemical Exploration》2009,100(2-3):163-168

Acid Mine Drainage (AMD) needs to be treated before it can be re-used or discharged in receiving water bodies due to the low pH, high salinity and high sulphate concentrations of the water. Several treatment methods are currently applied including chemical treatment (e.g. neutralisation of the low pH waters), physical treatment (e.g. reverse osmosis) and biological treatment to reduce the high sulphate concentration. When treating AMD biologically, sulphate reducing bacteria (SRB) reduce sulphate to sulphide, provided that a suitable and cost effective carbon and energy source is present. In the present study mine water was remediated biologically, using the degradation products of grass-cellulose, as carbon and energy sources for the sulphate reducing bacteria. A laboratory scale one stage anaerobic bioreactor (20 L volume) containing grass cuttings and biomass consisting of rumen fluid microorganisms and immobilized SRB, was initially fed with synthetic sulphate rich water and later with diluted AMD. The results indicated an average of 86% sulphate removal efficiency when feeding synthetic sulphate rich feed water to the reactor. When feeding diluted AMD, the highest sulphate removal efficiency was 78%. The sulphate removal was dependant on Chemical Oxygen Demand (COD) concentrations in the reactor. Increased COD concentrations were obtained when fresh grass was added to the reactor on a regular basis. Metal removal, especially iron, was observed due to the metal sulphide precipitates formed during biological sulphate removal.  相似文献   

Mineral photoelectrons and their implications for the origin and early evolution of life on Earth   总被引：1，自引：0，他引：1  

LU AnHuai  WANG Xin  LI Yan  DING HongRui  WANG ChangQiu  ZENG CuiPing  HAO RuiXia  YANG XiaoXue 《中国科学:地球科学(英文版)》2014,57(5):897-902

Energy is the key issue of all life activities.The energy source and energy yielding pathway are the key scientific issues of the origin and early evolution of life on Earth.Current researches indicate that the utilization of solar energy in large scale by life was an important breaking point of the early evolution of life on Earth and afterwards life gradually developed and flourished.However,in the widespread biochemical electron transfer of life activities,it is still not clear whether the electron source is sun or how electrons originated from sun.For billions of years,the ubiquitous semiconducting minerals in epigeosphere absorb solar energy,forming photoelectrons and photoholes.In reductive and weak acidic environment of early Earth,when photoholes were easily scavenged by reducing matters,photoelectrons were separated.Photoelectrons could effectively reduce carbon dioxide to organic matters,possibly providing organic matter foundation for the origin of life.Photoelectrons participated in photoelectron transfer chains driven by potential difference and transfer into primitive cells to maintain metabolisms.Semiconducting minerals,by absorbing ultraviolet,also protected primitive cells from being damaged by ultraviolet in the origin of life.Due to the continuous photoelectrons generation in semiconducting minerals and utilization by primitive cells,photoelectrons from semiconducting minerals’photocatalysis played multiple roles in the origin of life on early Earth,such as organic synthesis,cell protection,and energy supply.This mechanism still plays important roles in modern Earth surface systems.  相似文献   

Quantitative characteristics of microorganisms in permafrost at different depths and their relation to soil physicochemical properties     

Wei Zhang  XiaoPei Dong  GuangXiu Liu  GaoSen Zhang  XiuKun Wu  XiSheng Tai  HaoZhi Long  BaoGui Zhang 《寒旱区科学》2012,4(2):0127-0131

Microorganisms in permafrost can live in cold environments due to coadapted physicochemical processes in this environment. In this paper, the relation between microbial number and soil physicochemical properties at the headwaters area of the Urumqi River is analyzed by using fluorescence microscopy counting and oligo-culture techniques. In total, 20 samples from a 200-cm permafrost core were used as study materials. The study reveals that the number of culturable bacteria has a significantly positive correlation with soil water content, total carbon and total nitrogen concentrations, and a significantly negative correlation with soil pH value. In addition, the ratio of culturable bacteria to total cell number decreases with depths. The results demonstrate that the number of culturable bacteria in permafrost is closely correlated with soil physicochemical properties and depositional age.  相似文献   

青海湖嗜盐微生物筛选分离与生长特性     

沈国平  朱德锐  刘建  韩睿  龙启福 《湖泊科学》2012,24(2):313-319

为全面获得青海湖嗜盐菌种质资源,构建种群系统发育树和确定种群进化定位.采用高盐选择性培养基筛选,分离从20份青海湖水样中获得35株青海湖嗜盐微生物.耐盐梯度实验表明:水体中以中度嗜盐菌为主,约占62.85%,轻度嗜盐菌约占22.85%,非嗜盐菌与耐盐菌约占14.28%.青海湖中度嗜盐微生物能在10～45℃,pH 5.5～11.0的范围内生长,与地区盐碱化环境相关,具有嗜盐兼嗜碱微生物的特性.  相似文献   

Crescentiella morronensis (Crescenti) (incertae sedis) dominated microencruster association in Lower Cretaceous (lower Aptian) limestones from the Rarău Massif (Eastern Carpathians,Romania)     

《Cretaceous Research》2017

Analysis of eight outcrops from Rarău Massif (Eastern Carpathians, Romania) revealed a rich assemblage of encrusting organisms mostly of problematic biological affiliation within platform-margin facies – Crescentiella morronensis, Radiomura cautica, Koskinobullina socialis, Pseudorothpletzella schmidi, Lithocodium aggregatum and bacinellid structures, encrusting calcified sponges (Calcistella jachenhausenensis, Neuropora lusitanica) and foraminifera (Coscinophragma cribrosa). Orbitolinids, calcareous green algae and rudists assign an early Aptian age (Bedoulian) for the studied limestones. In terms of species variety, abundance and structural microfabrics the studied microencruster association show similarity with the Upper Jurassic communities from reef and peri-reefal sedimentary settings. Comparisons with such assemblages are emphasized here. The presence of the sporolithacean and peyssonneliacean red algae (Sporolithon rude and Polystrata alba) contributes also to the construction of superimposed crusts. Even if the known stratigraphic range of the microencrusters is not strictly limited to the late Jurassic, reports of comparable associations in Urgonian-type facies is poorly documented. Therefore, it exemplifies the resistance and flourishing events of many encrusters throughout the Mesozoic.  相似文献   

南极罗斯海区域可培养微生物分离鉴定及产低温酶能力初步筛选     

张丽珉  赵琳  丛柏林 《海洋学报》2018,40(8):152-164

为了利用南极微生物资源、探索南极罗斯海区域可培养微生物多样性，利用传统平板培养法对中国第33次南极科学考察采自南极罗斯海6个站位的海洋沉积物样品进行了细菌、真菌的分离培养。经细菌16S rRNA、真菌ITS基因序列检测及系统发育分析，共获得5个属的36株细菌和6个属的29株真菌。其中嗜冷杆菌属为优势细菌类群，枝孢属为优势真菌类群。该结果表明南极罗斯海区域具有丰富的微生物多样性。细菌API 20 NE生理生化及真菌产胞外酶活性实验显示，分离得到的细菌和真菌绝大多数都有低温酶活性。研究结果为南极罗斯海区域可培养微生物的多样性认识和低温酶产生菌资源获取与开发利用提供了支撑。  相似文献   

深海来源微生物乙酰酯酶的酶学性质鉴定及拆分制备D-乳酸甲酯     

黄锦龙  张继福  胡洁莹  关见留  张云  孙爱君  胡云峰 《热带海洋学报》2018,37(4):38-44

D-乳酸及其酯是重要的手性药物中间体和手性化工产品。从南海深海芽孢杆菌Bacillus sp. SCSIO15029克隆到一个乙酰酯酶基因bae02030, 表达并鉴定该酶Bae02030的酶学性质。该酯酶的最适pH和最适温度分别为8.5和35℃, 其对多种有机溶剂和表面活性剂具有较好的耐受性。乙酰酯酶Bae02030能够通过水解拆分消旋乳酸甲酯来制备光学纯的D-乳酸甲酯。通过对拆分反应进行优化, 添加体积分数为60%的正庚烷能够改善乙酰酯酶Bae02030的光学选择性, 所制备的D-乳酸甲酯的对映体过量值(e.e._s)超过99%, 转化率(c)为56%。深海微生物来源的乙酰酯酶Bae02030作为生物催化剂在工业上制备手性药物中间体具有较好的应用潜力。  相似文献   

Determination of EC50 Values for Cu,Zn, and Cr on Microorganisms Activity in a Mediterranean Sandy Soil     

Vicen Carabassa  Xavier Domene  Oriol Ortiz  Evan A. N. Marks  Josep M. Alcaiz 《洁净——土壤、空气、水》2019,47(12)

Ecotoxicity of three potentially toxic metals (PTM) (Cu, Zn, and Cr) in a slightly acidic sandy soil is tested using the soil respiration test (OECD‐217) in order to determine EC₅₀ values for the carbon transformation activity of microorganisms. Addition of an organic amendment of Populus leaves is also crossed with metal spiking in order to investigate possible interaction with metal toxicity. Soil respiration is measured at day 1 and 28 after the soil spiking with the PTM to assess short‐term effects on soil microbial activity. Of the three metals tested, Cu shows the highest toxicity at the longest exposure times (day 28) and Zn shows a strong inhibitory effect in the short‐term (day 1), even though later toxicity diminish significantly. Cr is the least toxic studied PTM. Organic amendment outweighs any adverse effects of these metals, increasing soil respiration, even in the treatments with high doses of metals.  相似文献