皱纹盘鲍(Haliotis discus hannai Ino)和杂色鲍(Haliotis diversicolor Reeve)遗传多样性的RAPD研究   总被引：13，自引：0，他引：13       下载免费PDF全文

李太武  杨文新  宋林生  苏秀榕  杨志彪  郭皓 《海洋与湖沼》2003,34(4):444-449

于 2 0 0 1年 8月— 2 0 0 2年 7月 ,选用 2 0条能产生清晰可重复扩增产物的随机引物 ,对皱纹盘鲍 (HaliotisdiscushannaiIno)、杂色鲍 (HaliotisdiversicolorReeve)进行了RAPD分析 ,共检测到 2 1 3个位点 ,在群体之间和个体之间都存在差异。利用PopGen32软件计算各群体扩增位点的多态性比例、群体遗传杂和度和群体间的遗传距离 ,多态比例分别为 43.66%、5 3 0 5 % ,2个群体的平均杂合度为 0 .1 5 5 7、0 .1 686。结果表明皱纹盘鲍与杂色鲍的亲缘关系较远。  相似文献   

海带雌性原生质体的分离与培养     

秦松  王希华  童顺  曾呈奎 《海洋与湖沼》1995,26(S1):126-129

于1993年1月 - 1993年4月，为了制备遗传性纯一的基因工程受体，用酶法从海带雌配子体单性生殖系分离雌性单倍体原生质体。从皱纹盘鲍消化腺及消化器官提取出鲍酶，在25°C下、1％（W／V）的鲍酶与2％（W／V）的纤维素酶（Onozuka R－10）合用能有效解离单细胞和原生质体。海带雌性原生质体经2d培养再生了细胞壁。  相似文献   

不同食物与放养水层对九孔鲍生长的影响     

聂宗庆  王素平  陈才松 《广东海洋大学学报》2000,20(3):37-40

采用室内池子多层笼养殖方式 ,以加工海带、鲜江艹离 与 A,B2种片状饲料为饵料 ,试验了不同食物与不同放养水层对九孔鲍生长影响 ,结果表明 :( 1)从壳长增长上 ,海带与饲料 B近似 ,各为 11.3与 11.2 mm;其次为饲料 A,10 .2 mm;而江 艹离 最差 ,仅 7.7mm;( 2 )在体重增长上 ,以饲料 B最高 ,平均每只为 3.3g,其次为饲料 A与海带 ,平均每只 2 .9g,而江艹离 仅 1.8g;( 3)从试验期间所获体重增长比壳长增长看 ,两种饲料 ( B为 2 95,A为 2 84 mg/mm)均比两种海藻高 (海带为 2 57,江艹离 为 2 34mg/mm) ;( 4 )从垂直 11层笼子九孔鲍生长的比较 ,下层的好于上层 ,越是下层越好 ,投喂两种饲料在体重上差别更明显。  相似文献   

鲍高效养殖模式的研究     

郭新堂  马敬利 《海洋湖沼通报》1998,(3):43-50

本研究采取苗种强化培育、坑道培育大规格苗种、湖间带鲍参混养等相结合,并配套适当技术措施,促进鲍快速生长,缩短养殖周期一年左右,提高成活率15％以上,增加经济效益200％左右,形成鲍高效养殖的较理想模式。  相似文献   

鲍人工配合饲料中维生素C的稳定性     

吴永沛  刘年生  蔡慧农 《台湾海峡》1999,18(4):469-473

本文研究了鲍人工配合饲料的加工和应用过程中,维生素Ｃ的稳定性和损失规律。结果表明,在４５、６０、８０、１００、１２０℃的５种烘干温度条件下,饲料中ＶＣ的保留率分别为６１％、６２％、６５％、１９％和６％。烘干温度以８０℃最合适。统计分析表明,饲料中水份一与ＶＣ的损失程度呈高度相关性,烘干过程应尽快除去饲料中水份,。饲料在浸泡第水应用过程中,未经特别处理的饲料Ａ,浸泡２４ｈ后ＶＣ的损失率为９８％,而经  相似文献   

中国北方筏式养鲍技术研究     

王兴章  李云恩 《海洋湖沼通报》1999,(3):72-77

本文对筏式养鲍的海区条件、苗种饵料选择、病害防治、养殖技术及管理措施等方面进行了初步研究。本实验共筏养10万头鲍鱼,经三年养成,成活率达80％以上,年亩纯益达四万元,明显高于其他海水养殖品种。  相似文献   

Utilizing in vivo nuclear magnetic resonance spectroscopy to study sublethal stress in aquatic organisms     

Viant MR  Pincetich CA  Walton JH  Tjeerdema RS  Hinton DE 《Marine environmental research》2002,54(3-5):553-557

In vivo nuclear magnetic resonance spectroscopy (NMR) is a powerful technique for characterizing the sublethal actions of physical and chemical stressors in live, intact organisms. In particular, 31P NMR is ideal for observing perturbations to cellular energetics since critical metabolite concentrations, including phosphagens, ATP and inorganic phosphate (Pi), can be measured non-invasively and in real time. This technique's versatility is demonstrated not only in the diversity of organisms that can be studied, but also in its broad-ranging applicability to environmental research. Illustrative studies include the actions of copper in adult red abalone (Haliotis rufescens) and changes in energetically important metabolites in developing medaka embryos (Oryzias latipes). Advantages and disadvantages of in vivo NMR will be discussed.  相似文献   

潮间带垒石蒙网养鲍技术研究   总被引：1，自引：0，他引：1  

郭新堂  管相胜 《海洋湖沼通报》1997,(4):69-72

在潮间带进行垒石蒙网养鲍试验，４个岩礁区共设置１３６个石垛，放养壳长１．３￣３．０厘米的鲍５５．１万只，经１８个月养殖，共产商品鲍１９．２５４吨，平均亩产１３９７．８公斤，平均壳长６．８１厘米，成活率８９．６％，实现产值６９３．１４４万元，利润３５２．８１万元。该成果１９９６年被评为青岛市科技进步三等奖。  相似文献   

Continuing observation of disturbed reproductive cycle and ovarian spermatogenesis in the giant abalone, Haliotis madaka from an organotin-contaminated site of Japan   总被引：1，自引：0，他引：1  

Horiguchi T  Kojima M  Takiguchi N  Kaya M  Shiraishi H  Morita M 《Marine pollution bulletin》2005,51(8-12):817-822

Histological examination of gonads as well as chemical analysis of organotin compounds in tissues of the giant abalone, Haliotis madaka, was conducted to evaluate continuing endocrine disruption in abalone populations in Japan. Abalone specimens were collected from two different areas, Tsushima as a reference site and Jogashima as a site representative of declining abalone populations where serious organotin contamination had been observed, each month from January 1998 to March 1999. Scores were given to the development stages of reproductive cells in the ovary and testis. The degree of sexual maturation was evaluated by calculating the mean value of a histogram of these scores for the reproductive cells of each abalone. The temporal variations in degree of sexual maturation showed that female and male abalone from Tsushima matured synchronously, while those from Jogashima did not, which were similar to results of the previous study during September 1995–November 1996. Approximately 19% of the female abalone from Jogashima were masculinized with an ovo-testis, which was also similar to the result of the previous study. The masculinization of female abalone is reported to be induced by tributyltin (TBT) and triphenyltin (TPhT) from antifouling paints. Concentrations of the sum of butyltins (TBT, dibutyltin (DBT) and monobutyltin (MBT): ΣBTs) and the sum of phenyltins (TPhT, diphenyltin (DPhT) and monophenyltin (MPhT): ΣPhTs) in the muscle of abalone from Jogashima (n = 73) of 7.8 ± 9.0 ng/g wet wt. and 4.5 ± 6.8 ng/g wet wt., respectively, were significantly higher than those from Tsushima (n = 87) of 4.7 ± 4.9 ng/g wet wt. and 0.8 ± 1.7 ng/g wet wt., respectively (p < 0.05 for ΣBTs; p < 0.001 for ΣPhTs), although concentrations of TBT and TPhT in the muscle of abalone from Jogashima (n = 73) of 2.2 ± 2.5 ng/g wet wt. and 5.8 ± 5.1 ng/g wet wt., respectively, were insignificantly and significantly higher than those from Tsushima (n = 87) of 0.4 ± 0.6 ng/g wet wt. and 0.5 ± 0.9 ng/g wet wt., respectively, (p > 0.05 for TBT; p < 0.001 for TPhT). Thus, endocrine disruption as well as contamination by organotins in the giant abalone from Jogashima is still persisting.  相似文献   

鲍人工饲料研究新进展   总被引：5，自引：1，他引：5  

王素平  聂宗庆 《台湾海峡》1996,(Z1)

本文综述了国内外鲍人工饲料研究与生产应用的水平与现状，对配合饲料中主要营养物质的要求、添加剂与粘合剂等亦作了全面介绍。  相似文献