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鲍人工配合饲料中应用防腐剂效果的研究 总被引:1,自引:0,他引:1
在鲍人工配合饲料中添加苯甲酸、山梨酸、尼泊金丁酯等防腐剂,在实验室设置的模拟投喂水体中,以饲料中孽生的细菌总数作为饲料在腐败过程中的腐败程度指标,研究了上述3种防腐剂在鲍饵中单独使用、混合使用以及多种添加量水平使用情况下的抑菌防腐效果及其特点。结果表明,鲍饵中添加0.50‰~1.00‰的尼泊金丁酯,在模拟投喂水体中具有非常显著而持久的防腐效果,30℃下72h内的腐败程度仅相当于不添加防腐剂的对照组饲料在16~25h内的腐败程度。还采用简易的投喂对照实验,初步验证了添加0.50‰尼泊金丁酯的人工配合饲料投喂对鲍的安全性。作者分析和讨论了以饲料在腐败过程中孽生的细菌总数作为模拟投喂过程中饲料的腐败度指标的优越性。 相似文献
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海洋放线菌胞外多糖发酵工艺及其条件研究 总被引:1,自引:0,他引:1
以具有明显海洋特性的、产胞外免疫活性多糖的链霉菌2305菌株作为发酵菌种,对其胞外多糖的发酵工艺及其条件进行了研究,考察了不同碳源、氮源、水质、培养基初始pH值、发酵温度、通气量等因素对发酵菌种的生物量及胞外多糖产量等的影响,建立和优化了胞外多糖摇瓶发酵培养基配方、发酵工艺及发酵条件,胞外多糖产量达到4.58g/L,转化率达到6.90%。研究了发酵过程中菌体生物量、底物糖残余量、pH值、产物多糖积累量等的动态变化,分析讨论了2305菌株胞外多糖发酵的动力学类型。 相似文献
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Thermostable SOD is a promising enzyme in biotechnological applications. In the present study, thermophile Geobacillus sp. EPT3 was isolated from a deep-sea hydrothermal field in the East Pacific. A thermostable superoxide dismutase(SOD) from this strain was purified to homogeneity by steps of fractional ammonium sulfate precipitation, DEAE-Sepharose chromatography, and Phenyl-Sepharose chromatography. SOD was purified 13.4 fold to homogeneity with a specific activity of 3 354 U/mg and 11.1% recovery. SOD from Geobacillus sp. EPT3 was of the Mn-SOD type, judged by the insensitivity of the enzyme to both KCN and H2O2. SOD was determined to be a homodimer with monomeric molecular mass of 26.0 k Da. It had high thermostability at 50°C and 60°C. At tested conditions, SOD was relatively stable in the presence of some inhibitors and denaturants, such as β-mercaptoethanol(β-ME), dithiothreitol(DTT), phenylmethylsulfonyl fluoride(PMSF), urea, and guanidine hydrochloride. Geobacillus sp. EPT3 SOD showed striking stability across a wide p H range from 5.0 to 11.0. It could withstand denaturants of extremely acidic and alkaline conditions, which makes it useful in the industrial applications. 相似文献
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通过分析比较了人工配合饲料及天然海藻饲养的九孔鲍 (Haliotisdiversicolorsupertexta)肌肉的营养成分及氨基酸含量。同时 ,测定了贝壳中微量元素的含量。这将有助于了解喂养人工饲料和天然海藻饲料的九孔鲍营养生理代谢的特点。与此同时 ,对喂养海藻饲料所出现的贝壳裂嘴和中间破损的异常鲍 ,也作了微量元素分析 ,探讨生长异常的原因。1材料与方法1.1样品的采集1.1.1天然海藻组九孔鲍取自福建省漳浦县 ,大小壳长4cm左右。养成期约4个月 ,以摄食海带为主 ,养殖过程发现有少量鲍发生贝壳裂嘴或… 相似文献
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