两株南极枝孢霉属真菌Cladosporium sp.NJF4和NJF6的次级代谢产物研究          下载免费PDF全文

常俊男  田晓清  樊成奇  黄金昌  陆亚男  韩清华 《极地研究》2020,32(1):60-67

南极因其独特的自然环境成为潜在、重要的微生物资源库,是产生新型生物活性物质和先导化合物菌株的潜在种源地,南极微生物正在成为创新药物研究新的重要资源。虽然近年来对南极微生物次级代谢产物的研究逐渐增加,但与温带和热带微生物研究相比仍处于初级阶段。对从南极普里兹湾海洋沉积物中获得的两株枝孢霉属真菌Cladosporium sp. NJF4和NJF6进行次级代谢产物分离及结构鉴定,获得20个化合物。化合物结构类型包括甾醇(1)、倍半萜类(7—8)、生物碱类(9—14)、二酮哌嗪(2—5、15—17)、芳香酸(6、18—19)等,其中倍半萜类(7—8)为首次从枝孢霉属真菌中分离得到,以上研究将为丰富南极微生物次级代谢产物库奠定一定的研究基础。  相似文献   

Late Holocene relative sea level rise and the Neoglacial history of the Greenland ice sheet     

Antony J. Long  Sarah A. Woodroffe  Sue Dawson  David H. Roberts  Charlotte L. Bryant 《第四纪科学杂志》2009,24(4):345-359

In West Greenland, early and mid Holocene relative sea level (RSL) fall was replaced by late Holocene RSL rise during the Neoglacial, after 4–3 cal. ka BP (thousand calibrated years before present). Here we present the results of an isolation basin RSL study completed near to the coastal town of Sisimiut, in central West Greenland. RSL fell from 14 m above sea level at 5.7 cal. ka BP to reach a lowstand of ?4.0 m at 2.3–1.2 cal. ka BP, before rising by an equivalent amount to present. Differences in the timing and magnitude of the RSL lowstand between this and other sites in West and South Greenland record the varied interplay of local and non‐Greenland RSL processes, notably the reloading of the Earth's crust caused by a Neoglacial expansion of the Greenland Ice Sheet (GIS) and the subsidence associated with the collapse of the Laurentide Ice Sheet forebulge. This means that the timing of the sea level lowstand cannot be used to infer directly when the GIS advanced during the Neoglacial. The rise in Late Holocene RSL is contrary to recently reported bedrock uplift in the Sisimiut area, based on repeat GPS surveys. This indicates that a belt of peripheral subsidence around the current ice sheet margin was more extensive in the late Holocene, and that there has been a switch from subsidence to uplift at some point in the last thousand years or so. Copyright © 2008 John Wiley & Sons, Ltd.  相似文献   

长牡蛎碱性磷酸酶的分离纯化及部分性质研究   总被引：5，自引：1，他引：5  

李兴暖  韩雅莉  肖湘  邓瑞鹏  周小朋  管云雁  黄长江 《台湾海峡》2003,22(4):482-486

本文以长牡蛎为酶源提取材料,用正丁醇抽提,硫酸铵分级分离,DEAE-52和Sephadex G-100柱层析,得到一定纯度的碱性磷酸酶,提纯倍数为61.96,比活力达1.326U/mg.该酶的最适pH值9.6,最适温度38℃,初速度6.8μmol/(dm~3·min),米氏常数Km值为1.30mmol/dm~3.实验结果表明,Mg~(2+)对该酶有明显的激活作用;Zn~(2+)、Cu~(2+)、Hg~(2+)对该酶有明显的抑制作用.  相似文献   

高压技术在深海沉积物兼性嗜压菌的筛选和鉴定中的应用     

游志勇  汤熙翔  肖湘 《台湾海峡》2007,26(4):555-561

通过自行改进的高压培养罐及高压设备,对深海沉积物进行可培养微生物的筛选,获得6株具有较强耐受压力的细菌.16SrDNA的测序结果表明这些细菌分别属于6个不同的菌属.压力生长试验的结果表明这6株细菌在40MPa的条件下仍然具有较强的生长能力,属于兼性嗜压菌.对不同压力下生长的细菌做显微镜检,结果显示,除了一株芽孢杆菌在40MPa下的菌体形态发生了明显的变化外,其它5株细菌在压力条件下的菌体的分裂均没有受到明显的影响.  相似文献   

闽南近海尖头斜齿鲨的生殖生物学研究   总被引：12，自引：0，他引：12  

陈明茹  丘书院  杨圣云 《海洋学报》2001,23(3):92-98

尖头斜齿鲨(Scoliodon laticaudus)是暖水性小型鲨鱼,为有胎盘胎生种类.在闽南近海,其雄鱼的性成熟长度为382～448mm,雌鱼的性成熟长度为405～467mm,1994年5月至1995年4月的周年随机采样中,雌鱼在群体中所占比例为53.0%.雌鱼的妊娠开始于1～4月,同年的6～8月分娩,妊娠期为5～6个月.尖头斜齿鲨雌鱼每个子宫一次妊娠怀胚胎1～12尾,其中3～6尾者占67.8%.雌鱼一次妊娠产仔鱼2～24尾,平均9.1尾,其中6～13尾占70%;57%的母体左右子宫内胚胎数相同.胚胎数量随母体全长的增大而呈线性增加.雌性尖头斜齿鲨生殖周期约为2年,一生生殖2～3次,生殖能力较低.尖头斜齿鲨胚胎在分娩时全长138～142mm,与雌鱼性成熟长度的比为0.30～0.35,胚胎的雌雄比为1.17:1.同一子宫内及左右两子宫中的胚胎发育情况均基本相同.另外还讨论了鲨鱼资源的保护和管理问题.  相似文献   

Population dynamics of Callichirus major (Say, 1818) (Crustacea, Thalassinidea) on a beach in northeastern Brazil     

M.L. Botter-Carvalho  P.J.P. Santos  P.V.V.C. Carvalho 《Estuarine, Coastal and Shelf Science》2007,71(3-4):508-516

We describe the structure, reproductive cycle, fecundity, growth, and mortality of a harvested population of the ghost shrimp Callichirus major. Samples were collected at monthly intervals from September 1999 to October 2000 on an urban sandy beach (08°11′S 34°55′W) in northeastern Brazil. During this period the sex ratio did not differ significantly from 1:1 (0.98 M: 1 F). Minimum and maximum sizes of the Dorsal Oval were 2.59 and 12.19 mm for males and 4.46 and 12.62 mm for females, respectively. Ovigerous females were found throughout the period, except between August and September 2000. Maximum lifespan was estimated as 3.3 and 3.4 years for females and males, respectively. This northeastern population differed from others previously studied in southern and southeastern Brazil, in regard to sex ratio, maximum attained size, maturation size, period and duration of the reproductive cycle, and fecundity. We interpret these regional differences as evidence for over-fishing at the study site, and suggest that large-scale management plans for callianassid populations should use regional population parameters.  相似文献   

Diet of albacore Thunnus alalunga from the waters of Mauritius (western Indian Ocean) inferred from stomach contents and fatty acid analysis     

Z Dhurmeea  H Pethybridge  EV Romanov  C Appadoo  N Bodin 《African Journal of Marine Science》2020,42(2):131-140

The diet of albacore Thunnus alalunga from their spawning ground in the waters of Mauritius was investigated. The stomach contents of 249 albacore individuals, caught by industrial longliners and artisanal fishers, was analysed, and the dominant prey taxa were selected for lipid and fatty acid (FA) analysis. The FA profiles of prey were compared with those of liver tissue of spawning-capable and post-spawning female albacore, using multivariate analysis. Whereas stomach content analysis identified cephalopods and crustaceans as the dominant prey items in number, FA-profile analysis identified crustaceans and fishes as the most-frequently consumed prey of post-spawning female albacore. In contrast, the FA profiles of spawning-capable albacore and those of prey showed very low similarity. Analysis of the prey suggests that although cephalopods, crustaceans and fishes could all provide albacore with the required lipids and FAs, cephalopod prey seem to be less desirable owing to their lower energy content (i.e. low in total lipid, triacylglycerol, 16:0, 18:0 and 18:1ω9). Instead, the most beneficial food appeared to be locally available fishes, which are more energy-rich and have a higher docosahexaenoic acid/ eicosapentaenoic acid ratio. These results provide new information on the trophic ecology of albacore, promote our understanding of the importance of prey type for successful reproduction of albacore, and highlight the advantages of FA profiling to study diet.  相似文献   

The role of schizogenesis in population dynamics of Timarete filigera (Polychaeta: Cirratulidae): 2-years observations in the Port of Bari (South Adriatic Sea)     

Miriam Gherardi  Margherita Sciscioli  Elena Lepore  Giuseppina Todisco  & Adriana Giangrande 《Marine Ecology》2007,28(2):306-314

Different aspects of reproductive biology of the polychaete Timarete filigera (Delle Chiaje) were studied during a period of 2 years (from September 2002 to August 2003 and from September 2004 to August 2005). Timarete filigera specimens were collected monthly in the port of Bari (South Adriatic Sea, Mediterranean Sea). For population analysis, regenerating and entire specimens were separately analyzed, the regenerating individuals were assigned to different categories related to the region of the body from which they derived. Regenerating individuals were present throughout the year. There were three to four times more individuals deriving from the middle part than the anterior and posterior regenerating portions. From the high percentage of regenerating individuals observed in the T. filigera population, it can be supposed that this species reproduces mainly asexually by schizogenesis. However, histological analysis revealed the presence of female gametes in a few individuals. In contrast, males were never collected, probably due to the limited number of reproductive individuals collected. The abundance has shown wide fluctuations during the year, with a decrease from Winter to Spring. The analysis of the size‐frequency histograms has shown a growing phase of whole specimens from Spring to Winter. The life cycle of the investigated species is discussed with the hypothesis that strategies could be linked to the colonized environments, agamic reproduction coupled with sexual reproduction assures the presence and expansion of the population after the colonization by a limited number of larvae.  相似文献   

Seasonality and Reproductive Phenology of Algae Inhabiting Littoral Pools in the Western Mediterranean     

Lisandro  Benedetti-Cecchi Francesco  Cinelli 《Marine Ecology》1993,14(2):147-157

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四川椎实螺属一新种(肺螺亚纲:基眼目,椎实螺科)     

金志良  刘月英 《海洋科学集刊》1997,(39):9-14

作者1987年于四川省乐山市大渡河尾段发现椎实螺属一新种,现命名为乐山椎实螺(Lymnaea leshanensis sp.nov.)。该种与日本琵琶湖产的 Lymnaea onychia 相似,但贝壳的形态、齿式、齿形、两性生殖系统的诸器官、外套膜的色带、个体大小、生活环境和动物区系均有所不同。前者属东洋区系,后者属古北区系。  相似文献