Ontogeny of the lymphoid organs of Japanese flounder, Paralichthys olivaceus     

Liu Yun  Jiang Guoliang  Zhang Shicui 《中国海洋大学学报(英文版)》2004,3(2):161-165

Histological study on the ontogeny of the lymphoid organs, kidney, thymus and spleen of Japanese flounder, Paralichthys olivaceus, from hatching to 40 d was carried out. The pronephric kidney duct appeared early in hatching although the primordial haemopoietic stem cells were observed within a week after hatching. The spleen was first seen after 8d of hatching. The thymus appeared after 15d, situated near the pronephric kidney. Small lymphoid cells appeared during the later phase of the post-larval stage in the sequence of thymus, kidney and spleen. During the 40d of observations, there were no distinct inner or outer zones in thymus and no red or white pulp in spleen. These results suggest that the nonspecific defense immune system plays a very important role in the early larval stage of Japanese flounder.  相似文献   

Chromosome Behavior of Heat Shock Induced Triploid in Fenneropenaeus Chinensis     

张晓军  李富花  相建海 《中国海洋湖沼学报》2003,21(3):222-228

INTRODUCTIONTriploidanimalsareusefulbecausetheyaresterile ,characterizedbypositivegrowthinthere productiveseason ,usefulfortesting ,forintroductionofnon nativespecies ,andforprotectionofde velopedstrains.Theinductionoftriploidyhadbeenreportedinmanyaquaculturespecies .Triploidshavebeensuccessfullyinducedinshrimpusingtemperatureshockorchemicalshock (CB ,6 DMAP)in 4species :Sicyoniaingentis (Xiangetal.,1 991 ) ,Fenneropenaeuschinensis (Xiangetal.,1 992 ;Daietal.,1 993;Baoetal.,1 993;Li…  相似文献   

CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES     

郭华荣  张士璀  李红岩  童裳亮  相建海 《中国海洋湖沼学报》2002,20(3):226-231

Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.  相似文献   

三倍体合浦珠母贝不育性研究     

何毛贤  林岳光  姜卫国 《热带海洋学报》1996,(2)

对三倍体合浦珠母贝Ｐｉｎｃｔａｄａｍａｒｔｅｎｓｉｉ（Ｄ．）春、秋两繁殖盛期的生殖腺进行了组织学观察，并对其配子形成及受精、发育进行了研究。结果表明：合浦珠母贝三倍体的生殖腺发育差，生殖细胞数量少，极少个体能见到成熟卵母细胞和精子；三倍体的配子能完成受精作用，能发育至直线铰合期。配子多为非整倍体，其染色体数目多为１４—２８，胚胎染色体数目主要为２８—４２，故胚胎不能正常发育。因此，三倍体合浦珠母贝是不育的．  相似文献   

杂交鲆(牙鲆♀×夏鲆♂)胚胎发育的初步观察     

于道德  肖志忠  徐世宏  马道远  李军 《海洋科学》2007,31(2):55-60

通过人工调控促使亲本牙鲆(Paralichthys olivaceus)♀×夏鲆(Paralichthys dentatus)♂的性腺发育,人工干法授精获得杂交受精卵。杂交受精卵在温度为16.5~17.5℃,光照为100~400 lx,微充气条件下孵化。对杂交鲆胚胎发育全过程进行连续观察并与牙鲆及夏鲆的胚胎发育进行比较。结果表明:杂交鲆卵裂方式与其他大部分硬骨鱼类一样,属于盘状卵裂。受精后经过2 h 30 min进入2细胞期,7 h 45 min进入囊胚期,15 h 10 min进入原肠胚阶段,25 h 30 min进入神经胚期,49 h左右进入尾芽期。授精后63 h左右发现初孵仔鱼,68h左右50%的仔鱼孵出。在杂交鲆的胚胎发育中,主要有2个发育特征与母本类似而与父本不同:视囊的出现要早于体节的分化;孵化前,仔鱼在卵膜内环绕卵黄囊不到1周。但是在其孵化时间上却与父本相似。  相似文献   

利用牙鲆鳃细胞系分离和培养淋巴囊肿病毒   总被引：1，自引：4，他引：1  

吕宏旭  汪岷  李红岩  郭华荣  张士璀  包振民  周丽 《中国海洋大学学报(自然科学版)》2003,33(2):233-239

本文利用牙鲆鳃细胞系进行了养殖牙鲆淋巴囊肿病毒的分离及培养 ,并通过电镜对培养细胞中淋巴囊肿病毒的形态及感染循环进行了初步研究。将病鱼的淋巴囊肿组织无菌滤液接种牙鲆细胞系 ,细胞出现了明显的细胞病变 ( Cytopathic effect,CPE)。电镜观察在培养细胞的胞质中有病毒的包涵体 ,胞质中散在 6角形、5角形或圆形的病毒粒子 ,大小为 10 0～ 140 nm之间。在感染细胞的线粒体中也存在大量的病毒颗粒。  相似文献   

皱纹盘鲍三倍体生长的初步研究   总被引：13，自引：0，他引：13  

孙振兴  宋志乐 《海洋湖沼通报》1992,(4):70-75

将用低温休克法和细胞松弛素B诱导法,获得的皱纹盘鲍(Haliotis discus hannai Ino)三倍体诱导群与二倍体的个体,在室内同样条件下饲育,对其生长情况进行了比较,结果表明:(1)三倍体与二倍体的当年稚鲍在壳长和体重的增长上无明显差异。(2)三倍体与二倍体的2龄鲍,在第二年前期生长无明显差异,但从第二年后期开始,三倍体的壳长、体重增长逐渐优于二倍体。(3)统计学分析表明,三倍体与二倍体的3龄鲍在体重、软体部和足肌的增长方面,存在着明显差异。(4)在室内饲育19个月的鲍,三倍体诱导群的壳长比二倍体大10.2％,体重比二倍体大20.1％,足肌湿重比二倍体大17.6％。  相似文献   

Metazoan parasites of flounder Platichthys flesus (L.) along a transect from the southwestern to the northeastern Baltic Sea     

Koie  M. 《ICES Journal of Marine Science》1999,56(2):157-163

  相似文献   

石鲽鱼人工育苗技术研究   总被引：1，自引：0，他引：1  

王立超  刘增华  王远洋  高永刚  连岩  张志新  倪高田 《海洋科学进展》2001,19(1):55-59

根据石鲽鱼的生物学特性 ,对亲鱼的选择培育、人工催产、挤卵、受精孵化和仔稚鱼的培育技术 ,进行试验探索。在室内人工控制水温、光照、饵料投喂的条件下 ,较为系统地研究了石鲽鱼的发育和变态及其与理化因子的关系 ,探索了人工育苗的关键技术 ,突破了过去单纯依赖采捕自然苗进行人工养殖的方式 ,为实现室内全人工育苗及养成产业化生产 ,解决苗种来源途径提供了技术保证  相似文献   

华贵栉孔扇贝三倍体的研究   总被引：2，自引：1，他引：2  

曾志南  陈木等 《台湾海峡》1995,14(2):155-162

本文报道了用细胞松驰素B、高温和低温休克等方法诱导华贵栉孔扇贝（Chlamys nobilis）三倍体的实验结果。在26.0～28.0℃条件下，选用不同浓度的细胞松驰素B0.1、0.3、0.5、0.7和1.0mg/dm^3，在受精后15min，处理受精卵15min，三倍体诱导率分别为12.5%、37.5%、68.0%、66.6%和81.0%，其中0.5～1.0mg/dm^3浓度的细胞松驰素B对改变倍性最有效。高温休克选用31.0、33.0、35.0和37.0℃处理受精卵，最高三倍体诱导率达46.7%（33.0℃,处理15min）。低温休克选用6.0、8.0、10.0和12.0℃处理受精卵，最高三倍体诱导率为40.7%（10.0℃，处理20min）。实验结果表明，随着处理强度的增大和处理时间的延长，三倍体诱导率增高、但孵化率下降，畸型率上升。用高温休克处理的实验组幼虫生长速度较对照组快。作者认为，在商业性生产中，温度休克是诱导扇贝三倍体的一种简便、有效的方法。文中还就细胞松驰素B与温度休克诱导三倍体的机制进行了讨论。  相似文献