Use of Sonication for Enhanced Sampling of Attached Microbes from Groundwater Systems     

Murray Close  Phil Abraham  Judith Webber  Eliza Cowey  Bronwyn Humphries  Graham Fenwick  Simon Howard  Kimberly Huynh  Travis Grace  Pierre-Yves Dupont  Louise Weaver 《Ground water》2020,58(6):901-912

The vast majority of microorganisms in aquifers live as biofilms on sediment surfaces, which presents significant challenges for sampling as only the suspended microbes will be sampled through normal pumping. The use of a down-well low frequency sonicator has been suggested as a method of detaching microbes from the biofilm and allowing rapid sampling of this community. We developed a portable, easy to use, low-frequency electric sonicator and evaluated its performance for a range of well depths (tested up to 42 m below ground level) and casing types. Three sonicators were characterized in laboratory experiments using a 1 m long tank filled with pea gravel. These included a commercially available pneumatic sonicator, a rotating flexible shaft sonicator, and the prototype electric sonicator. The electric sonicator detached between 56 and 74% of microbes grown on gravel-containing biobags at distances ranging between 2 and 50 cm from the sonicator. The field testing comprises of a total of 55 sampling events from 48 wells located in 4 regions throughout New Zealand. Pre- and post-sonication samples showed an average 33 times increase in bacterial counts. Microbial sequence data showed that the same classes are present in pre- and post-sonicated samples and only slight differences were seen in the proportions present. The sampling process was rapid and the significant increases in bacterial counts mean that microbial samples can be quickly obtained from wells, which permits more detailed analysis than previously possible.  相似文献   

Effect of Drain Pipes on Uplift Force and Exit Hydraulic Gradient and the Design of Gravity Dams Using the Finite Element Method     

Aghdam  Ali Taheri  Salmasi  Farzin  Abraham  John  Arvanaghi  Hadi 《Geotechnical and Geological Engineering》2021,39(5):3383-3399

Geotechnical and Geological Engineering - The effects of diameter and location of drain pipes on the uplift force and exit hydraulic gradient for a gravity dam are investigated. A numerical model...  相似文献   

shinyNORRRM: A Cross-Platform Software to Calculate the CIPW Norm     

González-Guzmán  Reneé  Elizondo-Pacheco  Luis Alejandro  González-Roque  Abraham  Sánchez-Torres  Carlos Eduardo  Cárdenas-Muñoz  Kevin Samuel 《Mathematical Geosciences》2023,55(4):563-577

In this paper, a novelty-free software to assess an efficient CIPW Norm (± 0.006 wt.% in differences between input and output data) is presented. The package is available in the official repository for user-contributed R packages (CRAN: Comprehensive R Archive Network). The software is able to handle big data sets and considers minor and trace element compositions. The algorithm can calculate odd minerals in igneous rocks, such as cancrinite and calcite, adjust the Fe⁺³/Fe⁺² ratio in different standard approaches, and recalculate the compositions of the rocks in an anhydrous basis (100 ± 0.003 wt.% volatile-free adjusted). Furthermore, the package calculates several petrological parameters, and the graphical outputs are displayed following IUGS scheme standards. The prime aspect of shinyNORRRM is the symbiosis of native R functions with the R package’s shiny (Web Application Framework for R) to run the norm in a user-friendly interface. shinyNORRRM can be executed in any operating system and requires no previous programming knowledge, thus promising to be the universal computational program in this matter. The output data are printed in the standard comma-separated values (*.csv) format, which is highly compatible with general spreadsheet editors. In this work, the algorithm of our program is validated using already compiled whole-rock geochemical databases.

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Higher tritium concentrations measured in permafrost thaw lakes in northern Alberta          下载免费PDF全文

J. J. Gibson  S. J. Birks  Y. Yi 《水文研究》2016,30(2):245-249

Tritium concentrations were measured in a survey of 24 lakes, 15 wetlands, and 133 groundwaters in the oil sands region of northeastern Alberta and compared with both recent precipitation and precipitation sampled during the 1960s tritium peak caused by atmospheric thermonuclear weapons testing. Water samples from lakes included a group of 14 thaw lakes that had higher runoff attributed to melting of permafrost in peat plateaus within their watersheds. While tritium in all lakes was found to be intermediate between recent and 1960s concentrations, the thaw lakes were found to be significantly enriched in tritium compared with other lakes, as were unfrozen wetlands characterized by a thick sequence of low‐hydraulic conductivity peat. The results provide further evidence of different water sources to the thaw lakes and may indicate that melting of modern permafrost in part formed since the 1950s is occurring in these systems. Copyright © 2015 John Wiley & Sons, Ltd.  相似文献   

Linkages between spatio‐temporal patterns of environmental factors and distribution of plant assemblages across a boreal peatland complex          下载免费PDF全文

Jeremy A. Graham  Jeremy A. Hartsock  Dale H. Vitt  R. Kelman Wieder  John J. Gibson 《Boreas: An International Journal of Quaternary Research》2016,45(2):207-219

Here we examine the arrangement of plant species across an oligotrophic bog/poor fen peatland complex in the North American boreal plain and the relationships of these species to their physical and chemical environment. A semi‐uniform spatial sampling approach was utilized to describe the species assemblages, pore‐water chemistry and physical condition of 100 plots throughout a single peatland complex. Regardless of sharing the same ground cover of Sphagnum mosses, the remaining species separated into four distinct assemblages, each with unique indicators. These species groups along with associated chemical and physical factors are organized into four ecosites: bog, margin (edge) and two poor fen ecosites. The plant assemblages of this peatland have a complex relationship with numerous gradients, both physical and chemical, including depth to water table, shade, pH, nutrient and base cation. Rather than being homogenous across the landscape, most environmental variables exhibit distinct spatial patterns and do so in relationship to the plant assemblages, forming spatially distinct ecosites across the complex. Base cation concentrations play a smaller role than previously thought in differentiating these ecosites, and in addition to shade and depth to water table, nitrogen in the form of dissolved organic nitrogen was highly related to the placement of these ecosites. Many significant chemical factors appear related to evaporative water loss within the peatland complex, and these chemical factors are used to differentiate the ecosites. However, the mediation of evaporative water loss is due largely to self‐generated responses of the plant assemblages related to shade through plant morphology and peat acrotelm development related to depth to water table. We conclude that plant species and associated environmental gradients act together to form spatially distinct ecosites. The distribution of these ecosites within this large, environmentally complex peatland is largely controlled by differing self‐generated responses along the hydrotopographical gradient of differential water loss.  相似文献   

Editorial     

Chris Gibson 《The Australian geographer》2015,46(3):297-298

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Land use and disturbance effects on the dynamics of natural ecosystems of the Monte Desert: Implications for their management     

P.E. Villagra  G.E. Defossé  H.F. del Valle  S. Tabeni  M. Rostagno  E. Cesca  E. Abraham 《Journal of Arid Environments》2009,73(2):202-211

The complex interactions between human activity and natural processes determine non-linear dynamics in ecosystems that can difficult their management. Human settlements in arid lands contribute to the modification of disturbance regimes, including the introduction of new disturbances and the elimination of others. In consequence, they can alter the functional mechanisms that allow systems to overcome limiting factors, leading to desertification. In this revision, we evaluated the effects of the changes on disturbance regimes produced by the different forms of land transformation on the structure and function of ecosystems of the Monte Biogeographical Province, in Argentinean arid west. Two approaches were used: the analysis of land use history and the analysis of the effects of the main disturbances on the dynamics of different communities. We concluded that throughout the history of the Monte Desert, the joint action of natural and anthropic agents has resulted in complex dynamics that lead most area of the Monte to a moderate to severe status of desertification. The modification of the disturbance regime had strong consequences for several aspects of the dynamics of communities, such as species composition and diversity, water dynamics, soil conditions, trophic structure and productivity of Monte Desert ecosystems. However, disturbance regimes could be managed to promote favorable transitions in ecosystems and, therefore, could be a tool for optimizing productivity of agro-ecosystems, and recovering and conserving natural ecosystems.  相似文献   

Endogenous growth in channelized komatiite lava flows: evidence from spinifex-textured sills at Pyke Hill and Serpentine Mountain,Western Abitibi Greenstone Belt,Northeastern Ontario,Canada     

Michel G. Houlé  Sonia Préfontaine  Anthony D. Fowler  Harold L. Gibson 《Bulletin of Volcanology》2009,71(8):881-901

Spinifex-textured sills (i.e., veins) characterized by komatiitic magmas that have intruded their own volcanic-piles have long been recognized. For instance, in the early 1970s, Pyke and coworkers, in their classic work at Pyke Hill in Munro Township, noted that not all spinifex-bearing ultramafic rocks formed as lava flows, rather some were clearly emplaced as small dikes and sills. Several hypotheses have been proposed to explain spinifex-textured sills: intrusion into a cold host, filter pressing, or drainage of residual liquid. However, these do not satisfactorily explain the phenomenon. Field and petrographic observations at Pyke Hill and Serpentine Mountain demonstrate that spinifex-bearing komatiite sills and dikes were emplaced during channel inflation processes when new magma was intruded into a cooler, semi-consolidated but permeable cumulate material. Komatiitic liquids were intruded into the olivine cumulate rocks near the boundary between the spinifex and the cumulate zones of well-organized to organized komatiite flows. Spinifex-textured sills are generally tabular in morphology, stacked one above another, with curviplanar contacts sub-parallel to stratigraphy. Some sills exhibit complex digitated apophyses. Thinner sills typically have a random olivine spinifex texture similar, though generally composed of coarser crystals, to that of komatiite lava flows. Thicker sills exhibit more complex organization of their constituent crystals characterized by zones of random olivine spinifex, overlying zones of organized coarse spinifex crystals similar to those found in lava flows. They have striking coarse dendritic spinifex zones composed of very large olivine crystals, up to several centimetres long and up to 1 cm wide that are not observed in lava flows. Typically, at the sill margins, the cumulate material of the host flow is composed of euhedral to subhedral olivine crystals that are larger than those distal to the contact. Many of these margin-crystals have either concentric overgrowth shells or dendritic olivine overgrowths that grew from the cumulate-sill contact toward the sill interior. The dendrites grew on pre-existing olivine cumulate at the contact in response to a sharp temperature gradient imposed by the intrusion of hot material, whereas the concentric overgrowths formed as new melt percolated into the unconsolidated groundmass of the host-flow cumulate material. Spinifex-textured sills and dikes occur in well-organized to organized flows that are interpreted to have formed by “breakouts” above and peripheral to lava pathways (channels/conduits) as a result of inflation that accompanied voluminous komatiitic eruptions responsible for the construction and channelization of komatiitic flow fields. The spinifex-textured dikes and sills represent komatiitic lava that was originally emplaced into the channel roof during periods of episodic inflation that resulted in lava breakouts and was subsequently trapped in the “roof rocks” during periods of channel deflation. Accordingly, the occurrence of spinifex-textured sills and dikes may indicate proximity to, and aid in the identification and delineation of lava channel-ways that could potentially host Ni–Cu–(PGE) mineralization within komatiitic lava flow-fields.  相似文献   

A Queer Country? A case study of the politics of gay/lesbian belonging in an Australian country town     

Andrew Gorman-Murray  Gordon Waitt  Chris Gibson 《The Australian geographer》2008,39(2):171-191

The present paper examines the complex politics of gay/lesbian belonging through a case study of Daylesford, Victoria, an Australian country town. It contributes to two research bodies: gay/lesbian rural geographies and the politics of belonging. Daylesford hosts ChillOut, Australia's largest rural gay/lesbian festival, which provides a telling context for investigating gay/lesbian belonging in rural Australia. We use qualitative data from the 2006 ChillOut Festival, including interviews with local residents, newspaper commentaries, and visitors’ surveys, to explore how Daylesford has been constructed, imagined, and experienced as a ‘unique’ site of gay/lesbian belonging in rural Australia. We find that ChillOut crucially contributes to its wider reputation as a gay-friendly country town, but also, we argue, to the contested nature of gay/lesbian belonging. This was most powerfully demonstrated by the local council's refusal to fly the gay-identified rainbow flag on the Town Hall during the 2006 Festival and its subsequent banning of the display of all festival flags from that key public building. Because ChillOut was the catalyst for this protocol, the resolution was viewed as homophobic. Indeed, the homophobic and heterosexist rhetoric that ensued in the Letters to the Editor section of the local newspaper revealed some residents’ underlying antagonism towards ChillOut and the local gay/lesbian community. Moreover, appealing to a shared ‘Australian identity’ and associated normative ‘family values’, these letter writers deployed a multi-scalar politics of belonging, where a sense of gay/lesbian belonging to Daylesford at the local scale was contested by the assertion of a ‘more meaningful’ national scale of allegiance fashioned by heteronormativity.  相似文献   

The morphology and hydrology of small spring-dominated channels     

Ronald E. Griffiths  Diana E. Anderson  Abraham E. Springer   《Geomorphology》2008,102(3-4):511-521

Small, low order channels located in wet meadows along the Mogollon Rim of northern Arizona that receive the bulk of their flow from spring discharge exhibit a morphology that differs markedly from channels that receive the bulk of their flow from runoff. These small, spring-dominated channels generally have dense vegetation cover, vertical (or near vertical) banks with flat channel beds that are armored with clasts up to 60 mm. Clasts armoring the spring-dominated channels become mobile at 45 to 85% of the bankfull depth. The lack of fine-grained material in the bed of the spring-dominated channels reflects the small drainage size, lack of fine grain input from the spring, and winnowing affect of the consistent discharge. Minor amounts of large woody debris were present in some of the spring-dominated channels, however, unlike previous studies it does not appear to play a role in the spring-dominated channel morphology. Sinuosity values for spring-dominated channels averaged 1.19, while the average sinuosity values for the runoff-dominated channels, 1.08, were significantly lower. Measured width-to-depth ratios averaged 2.4 in the spring-dominated channels, much lower than the average ratio of 11.6 found for the runoff-dominated channels. The standard deviation of width-to-depth ratios was higher for runoff-dominated channels, reflecting a more variable channel profile. A third channel type, here referred to as hybrid channels, receive significant flow from both springs and runoff. These channels have characteristics that fall between spring-dominated and runoff-dominated channels.Elevation, gradient, organic matter content, and sediment grain size distribution of the wet meadows in which the channels are formed do not exhibit significant differences between channel types, suggesting that these factors are not responsible for the observed differences in channel morphologies. The major differences in controls on the channel morphology found between the spring-dominated and runoff-dominated channels are the discharge regime and the sediment input. The hydrology unique to the spring-dominated channels and the lack of fine-grained sediment input combine to create the observed differences.  相似文献