Use of Sonication for Enhanced Sampling of Attached Microbes from Groundwater Systems     

Murray Close  Phil Abraham  Judith Webber  Eliza Cowey  Bronwyn Humphries  Graham Fenwick  Simon Howard  Kimberly Huynh  Travis Grace  Pierre-Yves Dupont  Louise Weaver 《Ground water》2020,58(6):901-912

The vast majority of microorganisms in aquifers live as biofilms on sediment surfaces, which presents significant challenges for sampling as only the suspended microbes will be sampled through normal pumping. The use of a down-well low frequency sonicator has been suggested as a method of detaching microbes from the biofilm and allowing rapid sampling of this community. We developed a portable, easy to use, low-frequency electric sonicator and evaluated its performance for a range of well depths (tested up to 42 m below ground level) and casing types. Three sonicators were characterized in laboratory experiments using a 1 m long tank filled with pea gravel. These included a commercially available pneumatic sonicator, a rotating flexible shaft sonicator, and the prototype electric sonicator. The electric sonicator detached between 56 and 74% of microbes grown on gravel-containing biobags at distances ranging between 2 and 50 cm from the sonicator. The field testing comprises of a total of 55 sampling events from 48 wells located in 4 regions throughout New Zealand. Pre- and post-sonication samples showed an average 33 times increase in bacterial counts. Microbial sequence data showed that the same classes are present in pre- and post-sonicated samples and only slight differences were seen in the proportions present. The sampling process was rapid and the significant increases in bacterial counts mean that microbial samples can be quickly obtained from wells, which permits more detailed analysis than previously possible.  相似文献   

Petrology and oxygen isotopic compositions of clasts in HED polymict breccia NWA 5232          下载免费PDF全文

Katrina D. van Drongelen  Douglas Rumble III  Kimberly T. Tait 《Meteoritics & planetary science》2016,51(6):1184-1200

Northwest Africa (NWA) 5232, an 18.5 kg polymict eucrite, comprises eucritic and exogenic CM carbonaceous chondrite clasts within a clastic matrix. Basaltic clasts are the most abundant eucritic clast type and show a range of textures and grain size, from subophitic to granoblastic. Other eucritic clast types present include cumulate (high‐En pyroxene), pyroxene‐lath, olivine rich with symplectite intergrowths as a break‐down product of a quickly cooled Fe‐rich metastable pyroxferroite, and breccia (fragments of a previously consolidated breccia) clasts. A variable cooling rate and degree of thermal metamorphism, followed by a complex brecciation history, can be inferred for the clasts based on clast rounding, crystallization (and recrystallization) textures, pyroxene major and minor element compositions, and pyroxene exsolution. The range in δ¹⁸O of clasts and matrix of NWA 5232 reflects its origin as a breccia of mixed clasts dominated by eucritic lithologies. The oxygen isotopic compositions of the carbonaceous chondrite clasts identify them as belonging to CM group and indicate that these clasts experienced a low degree of aqueous alteration while part of their parent body. The complex evolutionary history of NWA 5232 implies that large‐scale impact excavation and mixing was an active process on the surface of the HED parent body, likely 4 Vesta.  相似文献   

Differences in the Feeding Ecology of Two Seagrass-Associated Snails     

Kimberly K. Holzer  José L. Rueda  Karen J. McGlathery 《Estuaries and Coasts》2011,34(6):1140-1149

Grazing by small epifauna on live seagrass leaves was formerly viewed as unimportant in controlling plant biomass and growth, instead researchers focused on the indirect benefits of small invertebrates that crop algal competitors. Recent evidence shows that the emerald nerite Smaragdia viridis preferentially ingests seagrass leaf tissue. In contrast, the button snail Modulus modulus feeds on epiphytes and periphyton coating the leaves. We conducted laboratory microcosm and field experiments to investigate how the different feeding preferences of these seagrass-associated snails affect turtlegrass Thalassia testudinum primary production. Data revealed that after 24 h S. viridis reduced foliar biomass (25%) and chlorophyll (30%) and injured the equivalent of 50% of daily seagrass growth per shoot. Conversely, M. modulus did not affect these variables. Our results emphasize that in subtropical seagrass communities not all small epifauna browse off leaf surfaces and some can have important direct negative impacts on their seagrass host.  相似文献   

Assessing the effects of catchment‐scale urban green infrastructure retrofits on hydrograph characteristics          下载免费PDF全文

Kimberly M. Jarden  Anne J. Jefferson  Jennifer M. Grieser 《水文研究》2016,30(10):1536-1550

Run‐off from impervious surfaces has pervasive and serious consequences for urban streams, but the detrimental effects of urban stormwater can be lessened by disconnecting impervious surfaces and redirecting run‐off to decentralized green infrastructure. This study used a before–after‐control‐impact design, in which streets served as subcatchments, to quantify hydrologic effectiveness of street‐scale investments in green infrastructure, such as street‐connected bioretention cells, rain gardens and rain barrels. On the two residential treatment streets, voluntary participation resulted in 32.2% and 13.5% of parcels having green infrastructure installed over a 2‐year period. Storm sewer discharge was measured before and after green infrastructure implementation, and peak discharge, total run‐off volume and hydrograph lags were analysed. On the street with smaller lots and lower participation, green infrastructure installation succeeded in reducing peak discharge by up to 33% and total storm run‐off by up to 40%. On the street with larger lots and higher participation, there was no significant reduction in peak or total stormflows, but on this street, contemporaneous street repairs may have offset improvements. On the street with smaller lots, lag times increased following the first phase of green infrastructure construction, in which streetside bioretention cells were built with underdrains. In the second phase, lag times did not change further, because bioretention cells were built without underdrains and water was removed from the system, rather than just delayed. We conclude that voluntary green infrastructure retrofits that include treatment of street run‐off can be effective for substantially reducing stormwater but that small differences in design and construction can be important for determining the level of the benefit. Copyright © 2015 John Wiley & Sons, Ltd.  相似文献   

Land tenure and REDD+: The good,the bad and the ugly     

Anne M. Larson  Maria Brockhaus  William D. Sunderlin  Amy Duchelle  Andrea Babon  Therese Dokken  Thu Thuy Pham  I.A.P. Resosudarmo  Galia Selaya  Abdon Awono  Thu-Ba Huynh 《Global Environmental Change》2013,23(3):678-689

A number of international donors, national governments and project proponents have begun to lay the groundwork for REDD+, but tenure insecurity – including the potential risks of land grabbing by outsiders and loss of local user rights to forests and forest land – is one of the main reasons that many indigenous and other local peoples have publicly opposed it. Under what conditions is REDD+ a threat to local rights, and under what conditions does it present an opportunity? This article explores these issues based on available data from a global comparative study on REDD+, led by the Center for International Forestry Research, which is studying national policies and processes in 12 countries and 23 REDD+ projects in 6 countries. The article analyses how tenure concerns are being addressed at both national and project level in emerging REDD+ programs. The findings suggest that in most cases REDD+ has clearly provided some new opportunities for securing local tenure rights, but that piecemeal interventions by project proponents at the local level are insufficient in the absence of broader, national programs for land tenure reform. The potential for substantial changes in the status quo appear unlikely, though Brazil – the only one with such a national land tenure reform program – offers useful insights. Land tenure reform – the recognition of customary rights in particular – and a serious commitment to REDD+ both challenge the deep-rooted economic and political interests of ‘business as usual’.  相似文献   

Soldier crab (Dotilla myctiroides) distribution,food resources and subsequent role in organic matter fate in Ao Tang Khen,Phuket, Thailand     

Kimberly K. Takagi  Phaothep Cherdsukjai  Izumi Mimura  Yuka Yano  Kanjana Adulyanukosol  Makoto Tsuchiya 《Estuarine, Coastal and Shelf Science》2010

This study aimed to elucidate Dotilla myctiroides (H. Milne-Edwards) food resources and how the soldier crab influences the organic matter composition on the intertidal flat. A preliminary assessment of D. myctiroides size, sex, and population distribution was conducted to clarify the characteristics of the inhabiting population. Overall, D. myctiroides size and abundance was normal across the tidal flat area. Comparison of different sediment types from the soldier crab habitat (surface sediments, igloos, burrow lining, burrow pellets, feeding pellets, and feces) using the fatty acid (FA) analysis technique revealed D. myctiroides removes organic matter via the significantly lower contributions of polyunsaturated FAs, essential FAs, and dinoflagellates in the feeding pellets. Dotilla myctiroides tissue analysis of the hepatopancreas and hindgut in individuals that had been starved for 24 h confirmed not only the consumption of dinoflagellates, but diatoms and macroalgae as well. In addition, the FA metabolism within the digestive system implied the ability of the soldier crab to synthesize highly unsaturated FAs (HUFAs) such as 20:4n–6, 20:5n–3 and 22:6n–3.  相似文献   

In pursuit of procedural justice: Lessons from an analysis of 56 forest carbon project designs     

Kimberly R. Marion Suiseeya  Susan Caplow 《Global Environmental Change》2013,23(5):968-979

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In Situ Chemical Oxidation: Permanganate Oxidant Volume Design Considerations          下载免费PDF全文

Scott G. Huling  Randall R. Ross  Kimberly Meeker Prestbo 《Ground Water Monitoring & Remediation》2017,37(2):78-86

Contaminant rebound and low contaminant removal are reported more frequently with in situ chemical oxidation than other in situ technologies. Although there are multiple causes for these results, a critical analysis indicates that low oxidant volume delivery is a key issue. The volume of oxidant injected is critical and porosity of the aquifer matrix can be used to estimate the pore volume. The total porosity (q_T) is the volume of voids relative to the total volume of aquifer material. The mobile porosity (q_M) is the fraction of voids that readily contributes to fluid displacement, and is less than q_T leading to smaller estimates of oxidant volume. Injecting low‐oxidant volume may result in inadequate oxidant distribution and postinjection dispersal within the radius of influence, insufficient oxidant contact and oxidant loading, and incomplete treatment; whereas, greater oxidant volume achieves a greater oxidant footprint and may involve risk that the injected oxidant may migrate into nontarget areas and displacement of contaminated groundwater. Design guidelines and recommendations are provided that could help achieve more effective technology deployment, reduce the role of heterogeneities in the subsurface, and result in greater probability the oxidant is delivered to the targeted treatment zone.  相似文献   

A density-dependent multi-species model to assess groundwater flow and nutrient transport in the coastal Keauhou aquifer,Hawai‘i,USA     

Okuhata  Brytne K.  El-Kadi  Aly I.  Dulai  Henrietta  Lee  Jonghyun  Wada  Christopher A.  Bremer  Leah L.  Burnett  Kimberly M.  Delevaux  Jade M. S.  Shuler  Christopher K. 《Hydrogeology Journal》2022,30(1):231-250

Hydrogeology Journal - Fresh groundwater is a critical resource supporting coastal ecosystems that rely on low-salinity, nutrient-rich groundwater discharge. This resource, however, is subject to...  相似文献   

Submarine Groundwater Discharge-Derived Nutrient Loads to San Francisco Bay: Implications to Future Ecosystem Changes     

Kimberly A. Null  Natasha T. Dimova  Karen L. Knee  Bradley K. Esser  Peter W. Swarzenski  Michael J. Singleton  Mark Stacey  Adina Paytan 《Estuaries and Coasts》2012,35(5):1299-1315

Submarine groundwater discharge (SGD) was quantified at select sites in San Francisco Bay (SFB) from radium (²²³Ra and ²²⁴Ra) and radon (²²²Rn) activities measured in groundwater and surface water using simple mass balance box models. Based on these models, discharge rates in South and Central Bays were 0.3?C7.4?m³?day^?1?m^?1. Although SGD fluxes at the two regions (Central and South Bays) of SFB were of the same order of magnitude, the dissolved inorganic nitrogen (DIN) species associated with SGD were different. In the South Bay, ammonium (NH ₄ ⁺ ) concentrations in groundwater were three-fold higher than in open bay waters, and NH ₄ ⁺ was the primary DIN form discharged by SGD. At the Central Bay site, the primary DIN form in groundwater and associated discharge was nitrate (NO ₃ ^? ). The stable isotope signatures (??¹⁵N_NO3 and ??¹⁸O_NO3) of NO ₃ ^? in the South Bay groundwater and surface waters were both consistent with NO ₃ ^? derived from NH ₄ ⁺ that was isotopically enriched in ¹⁵N by NH ₄ ⁺ volatilization. Based on the calculated SGD fluxes and groundwater nutrient concentrations, nutrient fluxes associated with SGD can account for up to 16?% of DIN and 22?% of DIP in South and Central Bays. The form of DIN contributed to surface waters from SGD may impact the ratio of NO ₃ ^? to NH ₄ ⁺ available to phytoplankton with implications to bay productivity, phytoplankton species distribution, and nutrient uptake rates. This assessment of nutrient delivery via groundwater discharge in SFB may provide vital information for future bay ecological wellbeing and sensitivity to future environmental stressors.  相似文献