Use of Sonication for Enhanced Sampling of Attached Microbes from Groundwater Systems     

Murray Close  Phil Abraham  Judith Webber  Eliza Cowey  Bronwyn Humphries  Graham Fenwick  Simon Howard  Kimberly Huynh  Travis Grace  Pierre-Yves Dupont  Louise Weaver 《Ground water》2020,58(6):901-912

The vast majority of microorganisms in aquifers live as biofilms on sediment surfaces, which presents significant challenges for sampling as only the suspended microbes will be sampled through normal pumping. The use of a down-well low frequency sonicator has been suggested as a method of detaching microbes from the biofilm and allowing rapid sampling of this community. We developed a portable, easy to use, low-frequency electric sonicator and evaluated its performance for a range of well depths (tested up to 42 m below ground level) and casing types. Three sonicators were characterized in laboratory experiments using a 1 m long tank filled with pea gravel. These included a commercially available pneumatic sonicator, a rotating flexible shaft sonicator, and the prototype electric sonicator. The electric sonicator detached between 56 and 74% of microbes grown on gravel-containing biobags at distances ranging between 2 and 50 cm from the sonicator. The field testing comprises of a total of 55 sampling events from 48 wells located in 4 regions throughout New Zealand. Pre- and post-sonication samples showed an average 33 times increase in bacterial counts. Microbial sequence data showed that the same classes are present in pre- and post-sonicated samples and only slight differences were seen in the proportions present. The sampling process was rapid and the significant increases in bacterial counts mean that microbial samples can be quickly obtained from wells, which permits more detailed analysis than previously possible.  相似文献   

Paleogene evolution and demise of the proto‐Paratethys Sea in Central Asia (Tarim and Tajik basins): Role of intensified tectonic activity at ca. 41 Ma     

Mustafa Y. Kaya  Guillaume Dupont‐Nivet  Jean‐Noël Proust  Pierrick Roperch  Laurie Bougeois  Niels Meijer  Joost Frieling  Chiara Fioroni  Sevin zkan Alt&#x;ner  Ezgi Vardar  Natasha Barbolini  Marius Stoica  Jovid Aminov  Mehmut Mamtimin  Guo Zhaojie 《Basin Research》2019,31(3):461-486

The proto‐Paratethys Sea covered a vast area extending from the Mediterranean Tethys to the Tarim Basin in western China during Cretaceous and early Paleogene. Climate modelling and proxy studies suggest that Asian aridification has been governed by westerly moisture modulated by fluctuations of the proto‐Paratethys Sea. Transgressive and regressive episodes of the proto‐Paratethys Sea have been previously recognized but their timing, extent and depositional environments remain poorly constrained. This hampers understanding of their driving mechanisms (tectonic and/or eustatic) and their contribution to Asian aridification. Here, we present a new chronostratigraphic framework based on biostratigraphy and magnetostratigraphy as well as a detailed palaeoenvironmental analysis for the Paleogene proto‐Paratethys Sea incursions in the Tajik and Tarim basins. This enables us to identify the major drivers of marine fluctuations and their potential consequences on Asian aridification. A major regional restriction event, marked by the exceptionally thick (≤ 400 m) shelf evaporites is assigned a Danian‐Selandian age (ca. 63–59 Ma) in the Aertashi Formation. This is followed by the largest recorded proto‐Paratethys Sea incursion with a transgression estimated as early Thanetian (ca. 59–57 Ma) and a regression within the Ypresian (ca. 53–52 Ma), both within the Qimugen Formation. The transgression of the next incursion in the Kalatar and Wulagen formations is now constrained as early Lutetian (ca. 47–46 Ma), whereas its regression in the Bashibulake Formation is constrained as late Lutetian (ca. 41 Ma) and is associated with a drastic increase in both tectonic subsidence and basin infilling. The age of the final and least pronounced sea incursion restricted to the westernmost margin of the Tarim Basin is assigned as Bartonian–Priabonian (ca. 39.7–36.7 Ma). We interpret the long‐term westward retreat of the proto‐Paratethys Sea starting at ca. 41 Ma to be associated with far‐field tectonic effects of the Indo‐Asia collision and Pamir/Tibetan plateau uplift. Short‐term eustatic sea level transgressions are superimposed on this long‐term regression and seem coeval with the transgression events in the other northern Peri‐Tethyan sedimentary provinces for the 1st and 2nd sea incursions. However, the 3rd sea incursion is interpreted as related to tectonism. The transgressive and regressive intervals of the proto‐Paratethys Sea correlate well with the reported humid and arid phases, respectively in the Qaidam and Xining basins, thus demonstrating the role of the proto‐Paratethys Sea as an important moisture source for the Asian interior and its regression as a contributor to Asian aridification.  相似文献   

Shapes of chondrules determined from the petrofabric of the CR2 chondrite NWA 801          下载免费PDF全文

Christopher R. J. Charles  Pierre-Yves F. Robin  Donald W. Davis  Phil J. A. McCausland 《Meteoritics & planetary science》2018,53(5):935-951

The approximately spherical shapes of chondrules has long been attributed to surface tension acting on ~1 mm melt droplets that formed and cooled in the microgravity field of the solar nebula. However, chondrule shapes commonly depart significantly from spherical. In this study, 109 chondrules in a sample of CR2 chondrite NWA 801 were imaged by X-ray computed tomography and best-fitted to ellipsoids. The analysis confirms that many chondrules are indeed not spherical, and also that the chondrules’ collective shape fabric records a definite 13% compaction in the host meteorite. Dehydration of phyllosilicates within chondrules may account for that strain. However, retro-deforming all chondrules shows that a large majority were already far from spherical prior to accretion. Possible models for these initial shapes include prior deformation of individual chondrules in earlier hosts, and, as suggested by previous authors, rotation of chondrules as they were solidifying, and/or “streaming” of molten chondrules by their differential velocities with their gaseous hosts after melting. More in situ 3-D work such as this study on a variety of unequilibrated chondrites, combined with detailed structural petrography, should help further constrain these models and refine our understanding of chondrite formation.  相似文献   

Lateral extrusion along the Altyn Tagh Fault,Qilian Shan (NE Tibet): insight from a 3D crustal budget          下载免费PDF全文

Feng Cheng  Marc Jolivet  Guillaume Dupont‐Nivet  Lin Wang  Xiangjiang Yu  Zhaojie Guo 《地学学报》2015,27(6):416-425

The lithospheric strike‐slip Altyn Tagh Fault has accommodated hundreds of kilometres of displacement between the Qaidam and Tarim blocks since its Eocene reactivation. However, the way the deformation is accommodated in the Qilian Shan and further east remains uncertain. Based on 360 km of north‐eastward migration of the relatively rigid Qaidam block along the Altyn Tagh Fault and 3D isovolumetric balancing of the crustal deformation within the Altyn Tagh Fault–Qilian Shan system, we demonstrate that 250 ± 28 km (43.8–49.4%) of N20E directed crustal shortening and an additional ~250–370 km of eastward motion of the Qilian Shan crust must be accounted for by strike‐slip faulting in the Qilian Shan and crustal thickening in the Qinling area, as well as by extension in the adjoining North China block graben systems.  相似文献   

Instability structures, synsedimentary faults and turbidites, witnesses of a Liassic seismotectonic activity in the Dauphiné Zone (French Alps): A case example in the Lower Pliensbachian at Saint-Michel-en-Beaumont     

Franoise Bergerat  Pierre-Yves Collin  Anne-Cline Ganzhorn  Franois Baudin  Bruno Galbrun  Isabelle Rouget  Johann Schnyder 《Journal of Geodynamics》2011,51(5):344-357

Instability structures, synsedimentary faults and turbidites have been studied in the Lower Pliensbachian succession of Saint-Michel-en-Beaumont, belonging to the Taillefer block, an ancient half-graben emplaced during the Liassic Tethyan rifting. Geometrical and mechanical analyses demonstrate that the instability structures occurred thanks to movements along spineless synsedimentary normal faults, when the turbiditic and limestone layers were already case-hardened and partly fractured by tension gashes even when the mudstones were still unlithified. Both the tension gashes and the synsedimentary faults are homogeneous in strike with the major regional faults and are in good agreement with the regional direction of extension for this period. The characters of the turbiditic beds, with erosive base, graded bedding, and incomplete Bouma sequence, are in favour of a seismic origin. Instability structures, spineless synsedimentary faults and turbiditic inflows are thus considered as seismites and interpreted as the result of high seismicity periods including some events with M > 5 in the general extensive ambiance of the Liassic Tethyan rifting. The analysis of the geometrical relationships between all these sedimentary features allows to distinguish the successive stage of occurrence of an instability structure, from the sedimentation of alternating marls and limestones, and sudden turbiditic inflows, then early case-hardening of the turbidites, until the important seismotectonic event generating the spineless normal faults, themselves triggering the fall of indurated blocks and locally the forming of breccias. The Ornon Fault, which constitutes the border of the Taillefer block, 15 km eastward, played a major role during the Liassic sedimentation and may represent the major seismic fault related to the seismites occurrence in the Beaumont basin.  相似文献   

Influence des rejets atmosphériques d'anhydride sulfureux sur la qualité de l'eau des lacs de la région de Rouyn‐Noranda*     

J. Dupont 《大气与海洋》2013,51(3):449-466

Résumé

En mars 1982, le ministère de l'Environnement du Québec a entrepris un échantillonnage physico‐chimique de 64 lacs de tête de la région de Rouyn‐Noranda. Les objectifs de l'étude étaient de déterminer la sensibilité des lacs de cette région et de délimiter l'aire d'influence de la plus importante source anthropique d'émission d'anhydride sulfureux au Québec.

Le traitement graphique et statistique des résultats, ainsi que l'application de modèles empiriques d'acidification ont permis de dégager les zones les plus vulnérables à l'acidification. L'application de ces techniques d'analyse a montré, entre autres, que les concentrations de sulfates en lac étaient beaucoup plus élevées à proximité de Rouyn‐Noranda où est située l'usine Mines Noranda Itée. De fait, la concentration de sulfates dans les lacs de cette région passe de 16 mg L^?1, aux alentours de Rouyn‐Noranda, à moins de 2 mg L^?1 en direction du nord‐est, alors qu'elle baisse à 8 mg L^?1 en bordure de la rivière des Outaouais. De plus, en faisant abstraction de la distribution en forme de cloche des concentrations de sulfates autour de Rouyn‐Noranda, l'étude a permis de faire ressortir la décroissance générale des sulfates du sud‐ouest vers le nord‐est.

Les points majeurs qui ressortent de cette étude sont la présence de lacs très acides à proximité de Rouyn‐Noranda et V extrême vulnérabilité des plans d'eau situés au sud et sud‐est de la ville. En effet, l'eau des lacs de la région du Témiscamingue est en général moins minéralisée et plus acide que celle des lacs au nord de cette région. Cette distinction est due à la nature géologique des substrats dans les bassins versants. Ainsi, les lacs sensibles à l'acidification sont principalement situés sur des bassins versants associés à des roches granitiques et gneissiques, comparativement aux roches volcaniques et aux dépôts d'argiles lacustres qui caractérisent les lacs aux eaux plus minéralisées.  相似文献   

What meta-analysis can tell us about vulnerability of marine biodiversity to ocean acidification?     

S. Dupont  N. Dorey  M. Thorndyke 《Estuarine, Coastal and Shelf Science》2010

Ocean acidification has been proposed as a major threat for marine biodiversity. Hendriks et al. [Hendriks, I.E., Duarte, C.M., Alvarez, M., 2010. Vulnerability of marine biodiversity to ocean acidification: a meta-analysis. Estuarine, Coastal and Shelf Science, doi:10.1016/j.ecss.2009.11.022.] proposed an alternative view and suggested, based on a meta-analysis, that marine biota may be far more resistant to ocean acidification than hitherto believed. However, such a meta-analytical approach can mask more subtle features, for example differing sensitivities during the life-cycle of an organism. Using a similar metric on an echinoderm database, we show that key bottlenecks present in the life-cycle (e.g. larvae being more vulnerable than adults) and responsible for driving the whole species response may be hidden in a global meta-analysis. Our data illustrate that any ecological meta-analysis should be hypothesis driven, taking into account the complexity of biological systems, including all life-cycle stages and key biological processes. Available data allow us to conclude that near-future ocean acidification can/will have dramatic negative impact on some marine species, including echinoderms, with likely consequences at the ecosystem level.  相似文献   

Orbital scale vegetation change in Africa     

Lydie Dupont 《Quaternary Science Reviews》2011,30(25-26):3589-3602

Palynological records of Middle and Late Pleistocene marine sediments off African shores is reviewed in order to reveal long-term patterns of vegetation change during climate cycles. Whether the transport of pollen and spores from the source areas on the continent to the ocean floor is mainly by wind or predominantly by rivers depends on the region. Despite the differences in transportation, accumulation rates in the marine sediments decline exponentially with distance to the shore. The marine sediments provide well-dated records presenting the vegetation history of the main biomes of western and southern Africa. The extent of different biomes varied with the climate changes of the glacial interglacial cycle. The Mediterranean forest area expanded during interglacials, the northern Saharan desert during glacials, and the semi-desert area in between during the transitions. In the sub-Saharan mountains ericaceous scrubland spread mainly during glacials and the mountainous forest area often increased during intermediate periods. Savannahs extended or shifted to lower latitudes during glacials. While the representation of the tropical rain forest fluctuated with summer insolation and precession, that of the subtropical biomes showed more obliquity variability or followed the pattern of glacial and interglacials.  相似文献   

Holocene environmental dynamics of south-eastern Brazil recorded in laminated sediments of Lago Aleixo     

Dirk Enters  Hermann Behling  Christoph Mayr  Lydie Dupont  Bernd Zolitschka 《Journal of Paleolimnology》2010,44(1):265-277

Environmental changes of the last 9,300 years were reconstructed by geochemical and pollen analyses of a 14-m-long, laminated sediment core from Lago Aleixo, south-eastern Brazil. Fossil pollen assemblages indicate open savannah vegetation (campo cerrado) and gallery forests until approximately 6,900 cal. BP. During that time, siderite laminae were deposited under anoxic conditions at the lake bottom. Then, increased rainfall and a shorter annual dry period allowed gallery forests and semi-deciduous forests to expand, leading to more closed cerrado vegetation. High-intensity rainfall events during this period are recorded as peaks in K and Ti concentrations. The sediment facies during this period consists of alternating layers of diatoms and minerogenic matter. C/N ratios imply that algae and perhaps soils, too, were the main contributors to sediment organic matter. Biogenic silica and δ¹³C_org variations indicate increasing primary productivity, which was related to higher nutrient flux from intensified leaching of soils, as shown by rising K/Al ratios. Around 800 cal. BP, a closed, semi-deciduous forest developed under present-day climate conditions. Slope stabilization diminished erosion processes in the catchment and caused reduced input of minerogenic matter into the basin. Human impact is evident in the topmost homogeneous sediments, as removal of the stabilizing forest cover amplified soil erosion. The continuous trend to more humid conditions during the Holocene probably reflects increased influence of the Amazon Basin as a moisture source. We conclude that the Lago Aleixo sediment archive was a sensitive recorder of environmental dynamics in tropical South America, which were mainly controlled by changes in precipitation patterns.  相似文献   

Modeling coastal tsunami hazard from submarine mass failures: effect of slide rheology,experimental validation,and case studies off the US East Coast     

Grilli  Stéphan T.  Shelby  Mike  Kimmoun  Olivier  Dupont  Guillaume  Nicolsky  Dmitry  Ma  Gangfeng  Kirby  James T.  Shi  Fengyan 《Natural Hazards》2017,86(1):353-391

Natural Hazards - We perform numerical simulations to assess how coastal tsunami hazard from submarine mass failures (SMFs) is affected by slide kinematics and rheology. Two types of two-layer SMF...  相似文献