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在遥感图像处理中,由于应用的需要,已经有很多算法可以用来融合高分辨率的全色影像和低分辨率的多光谱影像。本文阐述了小波变换的Mallat算法和a′Trous（多孔）算法,在此基础上,作者提出了一种基于多分辨率分析的灰度调制影像融合方法（MRAGM算法）,以SPOT全色影像与TM多光谱影像融合为例,详细给出了算法和解算步骤,最后分别用三种方法对SPOT高分辨率全色影像和TM低分辨率多光谱影像进行了融合,并且对融合后的影像从定性和定量方面都进行了评价,可以得出MRAGM方法比Mallat算法和a′Trous（多孔）算法的融合效果要好。 相似文献
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对2012—2013年从河北、福建和广东3个对虾养殖场采集的12份发生疑似早期死亡综合征/急性肝胰腺坏死综合征(EMS/AHPNS)的样品,采用组织病理学方法、套式RT-PCR检测方法以及快速灵敏检测试剂盒进行了检测。三地来源的养殖对虾样品症状均表现为肝胰腺颜色变淡,部分出现萎缩,空肠空胃,腹节肌肉略微白浊;组织病理切片结果表明,患病对虾肝胰腺血细胞浸润,肝胰腺盲管上皮细胞脱落,继发性细菌感染,淋巴器官和血淋巴细胞内存在核固缩、破裂和细胞质包涵体。按世界动物卫生组织(OIE)手册的套式RT-PCR方法检测,结果显示,黄头病毒(Yellow head virus,YHV)目的产物的阳性样品检出率为66.7%,该方法对这些样品的灵敏度可能很低。目的产物测序后经NCBI Blast,与已报道的YHV相似性为76.5%—89%;系统发育树显示这3个来源的样品位于同一个分支,与已知的YHV/鳃联病毒(gill-associated virus,GAV)株均不相同,其亲缘关系与YHV较近,而与GAV较远。用新研发的YHV快速高灵敏检测试剂盒检测,结果表明,所有样品均为YHV强阳性。以上表明患AHPNS的养殖对虾中存在一种YHV的新株型的感染,这也是我国首次检测到YHV的感染。 相似文献
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Edwardsiella tarda has become one of the most important emerging pathogens in aquaculture industry.Therefore,a rapid,reproducible,and sensitive method for detection and quantification of this pathogen is needed urgently.To achieve this purpose,we developed a TaqMan-based real-time PCR assay for detection and quantification of E.tarda.The assay targets the hemolysin activator HlyB domain protein of E.tarda.Our optimized TaqMan assay is capable of detecting as little as 40 fg of genomic DNA per reaction.A standard curve was generated from the threshold cycle values(y) against log10(E.tarda genomic DNA concentration) as x.The intra-and inter-assay coefficient of variation(CV) values were less than 2.06% and 1.05% respectively,indicating that the assay had good reproducibility.This method is highly specific to E.tarda strains,as it shows no cross-reactivity to Edwardsiella ictaluri,a member of the same genus,or to nine other fish-pathogenic bacteria species belonging to three other genera.This sensitive and specific real-time PCR assay provides a valuable tool for diagnostic quantitation of E.tarda in clinical samples. 相似文献
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集成变权与约束性模糊CA的城镇用地扩张情景模拟 总被引：1，自引：0，他引：1
引入变权理论、模糊理论、规模及空间约束等,构建了基于变权的约束性模糊元胞自动机(CA),用于城镇用地空间管制边界的模拟,并以太仓市区为例进行应用研究。结果显示,太仓市区城镇用地扩张首先通过内部填充,再进行外部扩张,体现了紧凑式发展模式,其发展方向符合"十一五"规划要求。研究表明,该模型不仅能解决城镇用地扩张的合理规模问题,同时也可灵活处理不同因素的作用程度及空间选择的模糊决策问题,故可更好地满足土地利用规划的需要。 相似文献
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Edwardsiella tarda is a major pathogen in aquatic environments that can cause heavy economic losses. An improved method for quick and accurate detection of E. tarda by loop-mediated isothermal amplification (LAMP) with two additional loop primers was developed by targeting the EsrB gene (EsrB-LAMP). In this method, the Mg 2+ concentration, reaction temperature, and reaction time were optimized to 8 mmol/L, 61°C, and 40 min, respectively. The detection limit with the EsrB gene was as low as 10 copies, which is 100 times more sensitive than that of conventional polymerase chain reaction (PCR). The EsrB-LAMP assay was shown more sensitive and rapid than previously reported LAMP assays targeting the hemolysin gene (hemolysin-LAMP) for detection of E. tarda. The EsrB-LAMP was also highly specific to E. tarda and had no cross-reaction with 13 other strains of bacteria. The assay can be carried out in a simple heating device and the EsrB-LAMP products can be visually detected by adding fluorescent dye to the reaction mixture. Taken together, the improved EsrB-LAMP diagnostic protocol has the potential for detection of E. tarda from indoor and outdoor samples. 相似文献
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