Evaluation of Cytotoxicity and Genotoxicity of Insecticide Carbaryl to Flounder Gill Cells and Its Teratogenicity to Zebrafish Embryos     

EY Manish Raj  GUO Huarong 《中国海洋大学学报(英文版)》2015,(2):362-374

In this study, we determined the cytotoxicity and genotoxicity of carbamate insecticide carbaryl to flounder gill(FG) cells and its teratogenicity to zebrafish embryos. The cytotoxicity of carbaryl to FG cells was determined with methods including MTT and neutral red uptaking(NRU), lactate dehydrogenase(LDH) releasing and Hoechst 33342 and propidium idodide(PI) double staining. Moderate cytotoxicity in a concentration-dependent manner was observed. The 24 h-IC50 value of 53.48 ± 1.21, 59.13 ± 1.19 and 46.21 ± 1.24 mg L-1 carbaryl was obtained through MTT, NRU and LDH assays, respectively. Double fluorescence staining demonstrated that carbaryl induced the death of FG cells mainly through necrosis. There was no significant genotoxicity found in the FG cells exposed to the highest testing concentration of carbaryl(20 mg L-1, P 0.05) as was demonstrated by Comet assay. Zebrafish embryos exposed to carbaryl at concentrations ≥10 mg L-1 displayed moderate toxic effects on the survival, spontaneous movement, hatching, heart rates of the embryos and their development, which were evidenced by yolk and pericardial sac edemas, body length reduction and tail flexure in time- and concentration-dependent manners at specific stages. The 24 h-, 48 h- and 96 h-LC50 values of carbaryl to zebrafish embryos were 41.80 ± 1.10, 17.80 ± 1.04 and 14.46 ± 1.05 mg L-1, respectively. These results suggested that carbaryl is moderately toxic to FG cells cultured in vitro and zebrafish embryos, and the FG cells were similar to zebrafish embryos in their sensitivity to carbaryl as 24 h-IC50 and LC50 indicated.  相似文献   

Effect of Freeze-Thaw Cycles on Mechanical Properties and Permeability of Red Sandstone under Triaxial Compression   总被引：1，自引：1，他引：0  

YU Jin  CHEN Xu  LI Hong  ZHOU Jia-wen  CAI Yan-yan 《山地科学学报》2015,(1):218-231

Geological disasters will happen in cold regions because of the effects of freeze-thaw cycles on rocks or soils, so studying the effects of these cycles on the mechanical characteristics and permeability properties of rocks is very important. In this study,red sandstone samples were frozen and thawed with 0,4, 8 and 12 cycles, each cycle including 12 h of freezing and 12 h of thawing. The P-wave velocities of these samples were measured, and the mechanical properties and evolution of the steady-state permeabilities were investigated in a series of uniaxial and triaxial compression tests. Experimental results show that, with the increasing of cyclic freeze-thaw times, the P-wave velocity of the red sandstone decreases. The number of freeze-thaw cycles has a significant influence on the uniaxial compressive strength, elastic modulus, cohesion, and angle of internal friction. The evolution of permeability of the rock samples after cycles of freeze-thaw in a complete stress-strain process under triaxial compression is closely related to the variation of the microstructure in the rock. There is a highly corresponding relationship between volumetric strain and permeability with axial strain in all stages of the stress-strain behaviour.  相似文献   

Anthropogenic Sulfate and Nitrate Signals in Snow from Bogda Glacier, Eastern Tianshan     

张晓宇  Ross Edwards 《中国地质大学学报(英文版)》2011,(4)

A 1.2 m snow pit was recovered on July 29th, 2009 from the Bogda Glacier, eastern Tianshan (天山). The sample site temperature of -9.6 ℃ indicates that the unique glaciochemical re-cord was well preserved and suitable for the reconstruction of air pollution levels in this previously un-explored region. Samples were analyzed for major ions (Na+, K+, Ca2+, Mg2+, NH4+, Cl-, SO42-, NO3-, HCOO-, and CH3COO-). NO3- and SO42- were characterized by significant high levels of pollution con-centration. Most air masses ...  相似文献   

Monitoring Glacial Shrinkage Using Remote Sensing and Site-Observation Method on Southern Slope of Kalik Mountain, Eastern Tian Shan, China     

王文彬  李开明  高建芳 《中国地质大学学报(英文版)》2011,(4)

Currently, one of the effective means in monitoring glacier change in regional scale is remote sensing and site-observation method. In this article, we present a study of comparing glacier area in 2005 derived from SPOT5 satellite image with area in 1972 derived from topographic maps. Moreover, Miaoergou (庙儿沟) flat-summit glacier is site observed to verify glacial change in regional scale. During the study period, glaciers located in the southern slope of Kalik (喀尔里克) Mountain re-duced their area by 12.3%. ...  相似文献   

Deposition Process of Dust Microparticles from Aerosol to Snow-Firn Pack on Glacier No. 1 in Eastern Tianshan Mountains, China     

尤晓妮  董志文 《中国地质大学学报(英文版)》2011,(4)

Samples were continuously collected from aerosol, fresh snow, and snow pits on Glacier No. 1 at Urumqi River source in eastern Tianshan (天山) Mountains. The deposition processes and the characteristics of mineral dust microparticles from aerosol to fresh snow, and then evolution to the snow pit were determined. Total dust microparticle concentration in the surface snow and aerosol showed a similar temporal variation trend, which was strongly associated with regional and local at-mospheric circulation in the ...  相似文献   

Noble Gas Diffusion Mechanism in Lunar Soil Simulant Grains:Results from ~4He~+ Implantation and Extraction Experiments     

付晓辉  邹永廖  郑永春  贺怀宇  欧阳自远 《中国地质大学学报(英文版)》2011,(5)

Experiments on ion implantation were performed in order to better characterize diffusion of noble gases in lunar soil.4He+ at 50 keV with 5×1016 ions/cm2 was implanted into lunar simulants and crystal ilmenite.Helium in the samples was released by stepwise heating experiments.Based on the data,we calculated the helium diffusion coefficient and activation energy.Lunar simulants display similar 4He release patterns in curve shape as lunar soil,but release temperatures are a little lower.This is probably a con...  相似文献   

Hybrid Sedimentation in Late Permian-Early Triassic in Western Sichuan Basin,China     

龙胜祥  吴世祥  李宏涛  白振瑞  马金钰  张华 《中国地质大学学报(英文版)》2011,(3)

Mixed carbonate-siliciclastic sedimentation is common in the Upper Permian-Lower Tri-assic in the western Sichuan(四川) basin.The extensional movement was strong in the Late Permian,resulting in differential uplifting of fault blocks.During this period,there was an extensive retrogres-sion.The Kangdian(康滇) ancient land progressive uplifted and enlarged,forming the major prove-nance of terrigenous clastics.In Changxingian age,mixed terrigenous-carbonate sedimentation was dis-tributed in the area between Dafeis...  相似文献   

Distribution of Arsenic in Sewage Irrigation Area of Pearl River Delta,China     

黄冠星  孙继朝  张英  荆继红  张玉玺  刘景涛 《中国地质大学学报(英文版)》2011,(3)

The aim of the present work is to investigate the distribution of arsenic(As) in sewage ir-rigation area,to deduce the migration and transformation mechanism of As in soil and groundwater,and to infer the source of As in soil and groundwater.This study is carried out in a sewage irrigation area of the Pearl River Delta,China.Surface water samples,soil samples,and groundwater samples from sewage irrigation area were analyzed for As and other elements.As contents in water samples were analyzed by hydride gene...  相似文献   

Comparison of Results of Recent Seismic Profiles with Tectonic Models of the North China Craton     

Timothy M Kusky 《中国地质大学学报(英文版)》2011,(2)

The geometry and timing of amalgamation of the North China craton(NCC) have been controversial,with three main models with significantly different interpretations of regional structure,geochronology,and geological relationships.The model of Zhao G C et al.suggests that the eastern and western blocks of the NCC formed separately in the Archean,and an active margin was developed on the eastern block between 2.5 and 1.85 Ga,when the two blocks collided above an east dipping subduc-tion zone.The model of Kusky ...  相似文献   

Formation Mechanism of High Quality Marine Source Rocks—Coupled Control Mechanism of Geological Environment and Organism Evolution     

腾格尔  胡凯  孟庆强  黄娟  付小东  谢小敏  仰云峰  高长林 《中国地质大学学报(英文版)》2011,(3)

High quality marine source rock (HQMSR) is the key prerequisite for medium to large hydrocarbon accumulations. However, the forming mechanism remains unclear. On the basis of the in-vestigation for the geodynamic setting of the Middle-Upper Yangtze during the Early Cambrian in dif-ferent spatial scales and the analysis of trace elements, the main controlling factors of the development of high quality marine source rock are discussed, with specific consideration of the burial rate of the organic matter. The ...  相似文献