Purification and Characterization of a Novel Hydrolase That Can Specifically Degrade the Polysaccharide Isolated from Green Seaweed Ulva prolifera     

LI Jiaxin  ZHANG Pei  HONG Liang  LIU Chenguang 《中国海洋大学学报(英文版)》2019,(1)

The extracellular polysaccharide hydrolase-producing strain EP-1 was isolated from seawater and identified as Paenibacillus pabuli. Furthermore, a homogeneous extracellular polysaccharide hydrolase from Paenibacillus pabuli EP-1 was purified by combining ion-exchange chromatography and size exclusion chromatography with a purification fold of 90.69 and recovery of 16.23%. Characterization of the purified polysaccharide hydrolase revealed a molecular mass of 38 k Da and optimum activity at 45℃ and pH 6.0. The polysaccharide hydrolase maintained its stability within a wide range of pH(3.0–12.0) and thermal stability when the temperature was below 50℃. The presence of Hg~(2+), Fe~(2+), Mn~(2+), Co~(2+) and SDS notably decreased hydrolase activity, and organic solvents such as formaldehyde, acetone, DMF and acetonitrile completely inhibited hydrolase activity. The purified hydrolase had no activity on agar, carrageenan, gellan gum, sodium alginate, or starch, but effectively hydrolyzed the polysaccharide from Ulva prolifera. The Km and Vmax values of this hydrolase were 43.84 mg m L~(-1) and 4.33 mg m L~(-1) min~(-1), respectively. The sequence analysis with quantitative time-of-flight mass spectrometry indicated that the hydrolase was an endoglucanase.  相似文献   

贵州省江口县硒、锗特色耕地资源分布规律研究          下载免费PDF全文

韩姚飞  沈小庆  张德实  洪万华 《贵州地质》2020,37(3):320-328

雨天城市道路交通拥堵频发，交通运行在道路网络各路段间的交互影响较晴天明显增强。本文针对传统出行时间预算模型应用的局限性，考虑雨天各路段间行车延误的相互影响，改进了走行时间方差的计算方法，提出一种雨天城市道路交通拥堵概率预测方法。选取南京市某行车路径进行验证，通过4种工况、3种模型走行时间的比较，发现考虑相关性的分段组合模型更接近整体路径分布情况，且拥堵概率预测准确率大于90%。研究结果表明：本文提出的预测模型能有效预测城市道路雨天拥堵情况，可为雨天道路适应性评价和交通管控提供依据。  相似文献   

The Effects of Ocean Acidification on Feeding and Contest Behaviour by the Beadlet Anemone <Emphasis Type="Italic">Actinia equina</Emphasis>     

Tess Olivia Bamber  Angus Charles Jackson  Robert Philip Mansfield 《Ocean Science Journal》2018,53(2):215-224

Increasing concentrations of atmospheric carbon dioxide are causing oceanic pH to decline worldwide, a phenomenon termed ocean acidification. Mounting experimental evidence indicates that near-future levels of CO₂ will affect calcareous invertebrates such as corals, molluscs and gastropods, by reducing their scope for calcification. Despite extensive research into ocean acidification in recent years, the effects on non-calcifying anthozoans, such as sea anemones, remain little explored. In Western Europe, intertidal anemones such as Actinia equina are abundant, lower trophic-level organisms that function as important ecosystem engineers. Changes to behaviours of these simple predators could have implications for intertidal assemblages. This investigation identified the effects of reduced seawater pH on feeding and contest behaviour by A. equina. Video footage was recorded for A. equina feeding at current-day seawater (pH 8.1), and the least (pH 7.9) and most (pH 7.6) severe end-of-century predictions. Footage was also taken of contests over ownership of space between anemones exposed to reduced pH and those that were not. No statistically significant differences were identified in feeding duration or various aspects of contest behaviour including initiating, winning, inflating acrorhagi, inflicting acrorhagial peels and contest duration. Multivariate analyses showed no effect of pH on a combination of these variables. This provides contrast with other studies where anemones with symbiotic algae thrive in areas of natural increased acidity. Thus, novel experiments using intraspecific contests and resource-holding potential may prove an effective approach to understand sub-lethal consequences of ocean acidification for A. equina, other sea anemones and more broadly for marine ecosystems.  相似文献   

Mesozoic mafic magmatism in North China: Implications for thinning and destruction of cratonic lithosphere   总被引：3，自引：0，他引：3  

Yongfei Zheng  Zheng Xu  Zifu Zhao  Liqun Dai 《中国科学:地球科学(英文版)》2018,61(4):353-385

The North China Craton (NCC) has been thinned from >200 km to <100 km in its eastern part. The ancient subcontinental lithospheric mantle (SCLM) has been replaced by the juvenile SCLM in the Meoszoic. During this period, the NCC was destructed as indicated by extensive magmatism in the Early Cretaceous. While there is a consensus on the thinning and destruction of cratonic lithosphere in North China, it has been hotly debated about the mechanism of cartonic destruction. This study attempts to provide a resolution to current debates in the view of Mesozoic mafic magmatism in North China. We made a compilation of geochemical data available for Mesozoic mafic igneous rocks in the NCC. The results indicate that these mafic igneous rocks can be categorized into two series, manifesting a dramatic change in the nature of mantle sources at ~121 Ma. Mafic igneous rocks emplaced at this age start to show both oceanic island basalts (OIB)-like trace element distribution patterns and depleted to weakly enriched Sr-Nd isotope compositions. In contrast, mafic igneous rocks emplaced before and after this age exhibit both island arc basalts (IAB)-like trace element distribution patterns and enriched Sr-Nd isotope compositions. This difference indicates a geochemical mutation in the SCLM of North China at ~121 Ma. Although mafic magmatism also took place in the Late Triassic, it was related to exhumation of the deeply subducted South China continental crust because the subduction of Paleo-Pacific slab was not operated at that time. Paleo-Pacific slab started to subduct beneath the eastern margin of Eruasian continent since the Jurrasic. The subducting slab and its overlying SCLM wedge were coupled in the Jurassic, and slab dehydration resulted in hydration and weakening of the cratonic mantle. The mantle sources of ancient IAB-like mafic igneous rocks are a kind of ultramafic metasomatites that were generated by reaction of the cratonic mantle wedge peridotite not only with aqueous solutions derived from dehydration of the subducting Paleo-Pacific oceanic crust in the Jurassic but also with hydrous melts derived from partial melting of the subducting South China continental crust in the Triassic. On the other hand, the mantle sources of juvenile OIB-like mafic igneous rocks are also a kind of ultramafic metasomatites that were generated by reaction of the asthenospheric mantle underneath the North China lithosphere with hydrous felsic melts derived from partial melting of the subducting Paleo-Pacific oceanic crust. The subducting Paleo-Pacific slab became rollback at ~144 Ma. Afterwards the SCLM base was heated by laterally filled asthenospheric mantle, leading to thinning of the hydrated and weakened cratonic mantle. There was extensive bimodal magmatism at 130 to 120 Ma, marking intensive destruction of the cratonic lithosphere. Not only the ultramafic metasomatites in the lower part of the cratonic mantle wedge underwent partial melting to produce mafic igneous rocks showing negative ε_Nd(t) values, depletion in Nb and Ta but enrichment in Pb, but also the lower continent crust overlying the cratonic mantle wedge was heated for extensive felsic magmatism. At the same time, the rollback slab surface was heated by the laterally filled asthenospheric mantle, resulting in partial melting of the previously dehydrated rocks beyond rutile stability on the slab surface. This produce still hydrous felsic melts, which metasomatized the overlying asthenospheric mantle peridotite to generate the ultramafic metasomatites that show positive ε_Nd(t) values, no depletion or even enrichment in Nb and Ta but depletion in Pb. Partial melting of such metasomatites started at ~121 Ma, giving rise to the mafic igneous rocks with juvenile OIB-like geochemical signatures. In this context, the age of ~121 Ma may terminate replacement of the ancient SCLM by the juvenile SCLM in North China. Paleo-Pacific slab was not subducted to the mantle transition zone in the Mesozoic as revealed by modern seismic tomography, and it was subducted at a low angle since the Jurassic, like the subduction of Nazca Plate beneath American continent. This flat subduction would not only chemically metasomatize the cratonic mantle but also physically erode the cratonic mantle. Therefore, the interaction between Paleo-Pacific slab and the cratonic mantle is the first-order geodynamic mechanism for the thinning and destruction of cratonic lithosphere in North China.  相似文献   

Water Temperature Dynamics in a Complex of Reservoirs and Its Effect on the Temperature Patterns of a Mountain River     

Łukasz Wiejaczka  Małgorzata Kijowska-Strugała  Paweł Pierwoła  Mariusz Nowak 《Water Resources》2018,45(6):861-872

The article presents the spatial and temporal dynamics of water temperature in two reservoirs on the upper course of the Dunajec River in the Polish Carpathians. It aims at presenting how spatial patterns and time evolution of water temperature in the reservoirs affect the water temperature in the river. The analysis is based on the results of water temperature measurements in hydrometric verticals in two reservoirs as well as in the river upstream and downstream of the reservoirs. The measurements were carried out in 2012 and 2013, in hydrometeorological conditions typical for each season of the year. Based on the measurements, it has been demonstrated that the complex of reservoirs affects the water temperature in the river over the year and the existence of the smaller lower reservoir may attenuate the cooling or heating effect of the main reservoir on the river.  相似文献   

太平洋深海沉积物来源的菌株Tenacibaculum sp.HMG1对孔雀石绿降解特性的研究     

曲武  洪国粦  赵晶 《海洋学报(英文版)》2018,37(6):104-111

本文在太平洋深海沉积物中分离得到一株孔雀石绿降解菌株，鉴定命名为Tenacibaculum sp.HMG1。通过菌株生长实验和高效液相色谱的研究表明， HMG1菌株可以在20 mg/L的孔雀石绿中维持较快的生长速率，并且在12 h内可降解98.8%的孔雀石绿，这证明该菌株具有很高的孔雀石绿耐受能力和降解活性。通过基因组测序在HMG1菌株发现一条过氧化物酶基因可能参与了孔雀石绿的降解，随后利用原核表达获得了相应的重组蛋白。实验表明，该重组过氧化物酶具有极强的活性，可在1000 mg/L的孔雀石绿中发挥降解功能。本文利用液相色谱-质谱联用（LC-MS）技术对孔雀石绿的菌株降解产物和重组酶降解产物进行鉴定，并基于鉴定结果推测了两种降解途径。结果发现两种降解方式存在共同的降解途径。此外，孔雀石绿降解条件的实验结果证明重组过氧化物酶可以在低温（20℃）、复杂的pH值（6.0–9.0）、高盐度（100 mmol/L）、金属离子和EDTA等反应条件下依旧维持很高的孔雀石绿降解活性。以上实验结果表明，HMG1菌株和重组过氧化物酶均在孔雀石绿污染生物修复方面具有很大潜力。  相似文献   

An interacting new holographic dark energy in the framework of fractal cosmology     

Ehsan Sadri  Martiros Khurshudyan 《Astrophysics and Space Science》2018,363(11):230

In this paper, we study an interacting holographic dark energy model in the framework of fractal cosmology. The features of fractal cosmology could pass ultraviolet divergencies and also make a better understanding of the universe in different dimensions. We discuss a fractal FRW universe filled with the dark energy and cold dark matter interacting with each other. It is observed that the Hubble parameter embraces the recent observational range while the deceleration parameter demonstrates an accelerating universe and a behavior similar to \(\Lambda \mbox{CDM}\). Plotting the equation of state shows that it lies in phantom region for interaction mode. We use \(\mathit{Om}\)-diagnostic tool and it shows a phantom behavior of dark energy which is a condition of avoiding the formation of black holes. Finally we execute the StateFinder diagnostic pair and all the trajectories for interacting and non-interacting state of the model meet the fixed point \(\Lambda \mbox{CDM}\) at the start of the evolution. A behavior similar to Chaplygin gas also can be observed in statefinder plane. We find that new holographic dark energy model (NHDE) in fractal cosmology expressed the consistent behavior with recent observational data and can be considered as a model to avoid the formation of black holes in comparison with the main model of NHDE in the simple FRW universe. It has also been observed that for the interaction term varying with matter density, the model generates asymptotic de-Sitter solution. However, if the interaction term varies with energy density, then the model shows Big-Rip singularity. Using our modified CAMB code, we observed that the interacting model suppresses the CMB spectrum at low multipoles \(l<50\) and enhances the acoustic peaks. Based on the observational data sets used in this paper and using Metropolis-Hastings method of MCMC numerical calculation, it seems that the best value with \(1\sigma \) and \(2\sigma \) confidence interval are \(\Omega _{m0}=0.278^{+0.008~+0.010} _{-0.007~-0.009}\), \(H_{0}=69.9^{+0.95~+1.57}_{-0.95~-1.57}\), \(r_{c}=0.08^{+0.02~+0.027}_{-0.002~-0.0027}\), \(\beta =0.496^{+0.005~+0.009} _{-0.005~-0.009}\), \(c= 0.691^{+0.024~+0.039}_{-0.025~-0.037}\) and \(b^{2}=0.035\) according to which we find that the proposed model in the presence of interaction is compatible with the recent observational data.  相似文献   

Sunspot Positions and Areas from Observations by Pierre Gassendi     

Mikhail Vokhmyanin  Nadezhda Zolotova 《Solar physics》2018,293(11):150

Solar activity behaviour on the eve of the Maunder minimum may provide important information on the period of further suppression of sunspot population. We analyse sunspot positions and areas in the 1630s extracted from rare drawings published by Pierre Gassendi in Opera Omnia. This work was published in two different editions, the first in Lyon and the second almost 70 years later in Florence. The drawings published in Lyon are found to be slightly different from those published in Florence, which produces a discrepancy in the position of spots of a few degrees, while sunspot group areas may differ by a factor of two. We reveal that the orientation of the drawings in the book is not always the same as might be seen in the telescope. We conjecture that the time of Gassendi’s observations covers the beginning of a new Schwabe cycle in the southern hemisphere. The differential rotation rate in the 1630s is also assessed and discussed.  相似文献   

Low resolution spectroscopy of selected Algol systems     

Shanti Priya Devarapalli  Rukmini Jagirdar  M. Parthasarathy  D. K. Sahu  Vijay Mohan  B. C. Bhatt  Vineet S. Thomas 《Astrophysics and Space Science》2018,363(4):69

The analysis of spectroscopic data for 30 Algol-type binaries is presented. All these systems are short period Algols having primaries with spectral types B and A. Dominant spectral lines were identified for the spectra collected and their equivalent widths were calculated. All the spectra were examined to understand presence of mass transfer, a disk or circumstellar matter and chromospheric emission. We also present first spectroscopic and period study for few Algols and conclude that high resolution spectra within and outside the primary minimum are needed for better understanding of these Algol type close binaries.  相似文献   

Understanding the Role of Mass-Unloading in a Filament Eruption     

J. M. Jenkins  D. M. Long  L. van Driel-Gesztelyi  J. Carlyle 《Solar physics》2018,293(1):7

We describe a partial filament eruption on 11 December 2011 that demonstrates that the inclusion of mass is an important next step for understanding solar eruptions. Observations from the Solar Terrestrial Relations Observatory-Behind (STEREO-B) and the Solar Dynamics Observatory (SDO) spacecraft were used to remove line-of-sight projection effects in filament motion and correlate the effect of plasma dynamics with the evolution of the filament height. Flux cancellation and nearby flux emergence are shown to have played a role in increasing the height of the filament prior to eruption. The two viewpoints allow the quantitative estimation of a large mass-unloading, the subsequent radial expansion, and the eruption of the filament to be investigated. A 1.8 to 4.1 lower-limit ratio between gravitational and magnetic-tension forces was found. We therefore conclude that following the loss-of-equilibrium of the flux-rope, the radial expansion of the flux-rope was restrained by the filamentary material until 70% of the mass had evacuated the structure through mass-unloading.  相似文献