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脊尾白虾的行为学观察研究 总被引:5,自引:0,他引:5
在高密度室内饲养条件下,观察了脊尾白虾(Exopalaemon carinicauda)的摄食、相残、运动、蜕皮、间隔、攻击和防御行为。试验结果表明,脊尾白虾摄食行为活跃,运动行为多样,在饥饿或个体较大差异条件下具有一定程度的相残行为,蜕皮行为表现出一定的特殊性,常经数天多次蜕皮行为特征后才能将旧壳蜕下,间隔行为不明显,攻击频率不高。另外,通过设置不同投喂组和投喂间隔时间观察比较了索饵率和相残率,结果表明,索饵率在第8小时已基本达到最高,相残率随投喂间隔时间的延长和空间密度的增大而升高。 相似文献
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白斑综合征病毒对脊尾白虾的致病性研究 总被引:1,自引:0,他引:1
2010年、2011年江苏沿海多数地方海水养殖中发生了脊尾白虾大规模死亡的现象,本课题通过对患暴发性流行病的脊尾白虾(Exopalaemon carinicauda)进行病毒分子生物学检测,发现病虾体内白斑综合征病毒(White Spot Syndrome Virus,简称WSSV)检测普遍呈阳性;应用发病虾组织制备的病毒粗提液,进行了人工感染实验,实验表明该病毒对脊尾白虾具有较强的致病性,可引起68.5%的死亡率。电镜结果表明,发病虾与感染病虾鳃及肝胰脏等组织器官都发生了相同的细胞病理变化,主要表现为细胞核内染色质边聚、线粒体肿胀、内嵴消失等一系列细胞病理变化,在其细胞核与细胞质中发现了具有单层囊膜结构的对虾白斑综合征病毒粒子,病毒粒子大小约为(130~170)nm×(305~405)nm,核衣壳呈子弹形,一端较细另一端较粗,完整病毒粒子由核心、衣壳和囊膜构成,分子生物学实验表明该病毒为WSSV。实验结果符合柯赫氏法则,可以初步推断WSSV感染脊尾白虾并与引发的大规模死亡有着直接关系。 相似文献
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Paralytic shellfish toxins(PSTs) are notorious neurotoxins that threaten public health and food safety worldwide.Although PST monitoring programs have recently been established throughout China, the profiles and variation of PSTs in important commercial clams(e.g., Mactra veneriformis, Ruditapes philippinarum, and Meretrix meretrix) along the Jiangsu Province coastline remain largely unexplored. In this study, a validated hydrophilic interaction liquid chromatography–tandem mass spectrometry(HILIC-MS/MS) method was used to examine PST profiles and levels in 540 clam samples from natural production areas along Jiangsu Province coastline during2014–2016. Although the PST levels(≤6.38 μg saxitotoxin equivalents(eq)/kg) were consistently below European Union regulatory limits(≤800 μg saxitotoxin eq/kg) during this time period, saxitotoxin, decarbamoylsaxitotoxin,and gonyautoxins 1 and 4 were detected, and nearly 40% of the samples were saxitotoxin-positive. The PST levels also varied significantly by seasons, with peak values observed in May during 2014–2016. This is the first systematic report of PSTs in clams from Jiangsu Province, and additional research and protective measures are needed to ensure the safety of clams harvested in this area. 相似文献
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