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Microbial communities in different gastrointestinal regions(stomach, foregut, midgut, and hindgut) of the northern snakehead C hanna argus(Cantor, 1842) were compared by polymerase chain reaction and partial 16 S r DNA sequencing. A total of 194, 140, 212, and 122 OTUs were detected in the stomach, foregut, midgut, and hindgut, respectively. Significant dif ferences were found in the Sobs, ACE, Shannon, and Simpson indices among samples(P 0.05). The gastrointestinal microbial community of C. argus consisted predominantly of Proteobacteria with either H alomonas, Shewanella, Plesiomonas, or Sphingomonas. Fusobacteria, Firmicutes, and Bacteroidetes also existed in the gastrointestinal tracts. However, significant diff erences were found in the compositions of microbial community among the four regions( P 0.05). Cyanobacteria and Spirochetes were significantly higher in the midgut and hindgut( P 0.05). Fusobacteria and Firmicutes were dominant in the hindgut and foregut, respectively(P 0.05). Proteobacteria was the lowest in the hindgut( P 0.05). At genus level, C etobacterium and Plesiomonas were significantly higher in the hindgut than in the other three samples( P 0.05). Clostridium and Prevotella were the highest in the midgut(P 0.05). Halomonas, Shewanella, and S phingomonas were the highest in the foregut( P 0.05). Paracoccus and Vibrio were the highest in the stomach. Several genera were only detected in certain regions, as follows: stomach, P aracoccus and Vibrio; foregut, Halomonas, Shewanella, and Sphingomonas; midgut, Clostridium and Prevotella; and hindgut, C etobacterium and Plesiomonas( P 0.05). At the species level, A cinetobacter rhizosphaerae was only detected in the stomach. P revotella copri and Clostridium perfring were not detected in the foregut and midgut, respectively, whereas Prevotella copri and Faecalibacterium pra were not detected in the hindgut. These findings provide valuable information on the microbial community in each gastrointestinal region of C. argus. Moreover, this study indicated that microbial community was not only related to rearing environment but also to the physico-chemical characteristics of each gastrointestinal region. 相似文献
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以初始体质量(8.77±0.16)g的大菱鲆(Scophthalmus maximus)幼鱼为研究对象,在室内流水系统中进行9周的养殖实验,比较分析饲料中3种不同类型的糖源(葡萄糖、蔗糖和糊精)对大菱鲆脂肪代谢相关酶活性和肌肉脂肪酸组成的影响。结果表明,不同类型的糖源显著影响肝脏中与脂肪合成相关的葡萄糖-6-磷酸脱氢酶(G6PD)和苹果酸酶(ME)的活性(P0.05)。饲料糖源为葡萄糖时,大菱鲆肝脏中G6PD和ME的活性显著高于其他处理组,而蔗糖和糊精处理组之间没有显著差异。对与脂肪分解代谢相关酶的活性分析表明,肠道脂肪酶的活性受饲料中不同糖源的影响显著(P0.05);肝脏脂蛋白脂酶的活性在糖源为葡萄糖时显著低于其他处理组(P0.05);不同糖源对肝脂酶的活性无显著影响(P0.05);糊精组的总脂酶活性显著高于葡萄糖组(P0.05)。葡萄糖组大菱鲆肌肉中的C16∶0和C18∶0的含量显著高于糊精组,而C16∶1和C18∶1n-9的含量显著低于糊精组(P0.05)。研究表明,饲料中不同类型的糖源对大菱鲆肌肉脂肪酸的影响集中在饱和脂肪酸和单不饱和脂肪酸上,而对多不饱和脂肪酸的影响有限。 相似文献
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低温条件下饲料的脂肪含量对石斑鱼生长的影响 总被引：2，自引：0，他引：2
为了研究在低温条件下饲料中的脂肪含量对石斑鱼生长的影响,制作脂肪含量为7.53%(G1)、9.52%(G2)、13.27%(G3)和16.64%(G4)的石斑鱼试验饲料,在闭路循环水16.1~21.3℃的低温下养殖石斑鱼,试验结束时称重并进行生物学指标和氨基酸的测定,结果表明低温对鱼类的生长性能产生了一定程度的影响,低温条件下,随着脂肪含量的升高,实验石斑鱼的增重率、肝脂率、肌脂率有明显程度的提高,而肝体比、脏体比相应的降低,摄食G1、G2实验饲料的石斑鱼为负增长,其肝脂率和肌脂率显著高于摄食G3、G4实验饲料的石斑鱼,而肝体比、脏体比却显著的低于后者,肌肉蛋白含量、水分及消化道指数无显著差异,死亡率随着脂肪含量的升高呈降低的趋势,各组石斑鱼肌肉的氨基酸组成差异不大,仅表现为酪氨酸随着脂肪添加量的增加减少的趋势,本实验说明了低温条件下适当增加饲料中的脂肪含量有利于保持石斑鱼的生长,同时为鱼类的越冬期及低温胁迫下的管理提供了1种可行的营养供给策略。 相似文献
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