Use of Sonication for Enhanced Sampling of Attached Microbes from Groundwater Systems     

Murray Close  Phil Abraham  Judith Webber  Eliza Cowey  Bronwyn Humphries  Graham Fenwick  Simon Howard  Kimberly Huynh  Travis Grace  Pierre-Yves Dupont  Louise Weaver 《Ground water》2020,58(6):901-912

The vast majority of microorganisms in aquifers live as biofilms on sediment surfaces, which presents significant challenges for sampling as only the suspended microbes will be sampled through normal pumping. The use of a down-well low frequency sonicator has been suggested as a method of detaching microbes from the biofilm and allowing rapid sampling of this community. We developed a portable, easy to use, low-frequency electric sonicator and evaluated its performance for a range of well depths (tested up to 42 m below ground level) and casing types. Three sonicators were characterized in laboratory experiments using a 1 m long tank filled with pea gravel. These included a commercially available pneumatic sonicator, a rotating flexible shaft sonicator, and the prototype electric sonicator. The electric sonicator detached between 56 and 74% of microbes grown on gravel-containing biobags at distances ranging between 2 and 50 cm from the sonicator. The field testing comprises of a total of 55 sampling events from 48 wells located in 4 regions throughout New Zealand. Pre- and post-sonication samples showed an average 33 times increase in bacterial counts. Microbial sequence data showed that the same classes are present in pre- and post-sonicated samples and only slight differences were seen in the proportions present. The sampling process was rapid and the significant increases in bacterial counts mean that microbial samples can be quickly obtained from wells, which permits more detailed analysis than previously possible.  相似文献   

Macrobenthic infaunal communities associated with deep‐sea hydrocarbon seeps in the northern Gulf of Mexico          下载免费PDF全文

Travis W. Washburn  Amanda W. J. Demopoulos  Paul A. Montagna 《Marine Ecology》2018,39(3)

There are thousands of seeps in the deep ocean worldwide; however, many questions remain about their contributions to global biodiversity and the surrounding deep‐sea environment. In addition to being globally distributed, seeps provide several benefits to humans such as unique habitats, organisms with novel genes, and carbon regulation. The purpose of this study is to determine whether there are unique seep macrobenthic assemblages, by comparing seep and nonseep environments, different seep habitats, and seeps at different depths and locations. Infaunal community composition, diversity, and abundance were examined between seep and nonseep background environments and among three seep habitats (i.e., microbial mats, tubeworms, and soft‐bottom seeps). Abundances were higher at seep sites compared to background areas. Abundance and diversity also differed among microbial mat, tubeworm, and soft‐bottom seep habitats. Although seeps contained different macrobenthic assemblages than nonseep areas, infaunal communities were also generally unique for each seep. Variability was 75% greater within communities near seeps compared to communities in background areas. Thus, high variability in community structure characterized seep communities rather than specific taxa. The lack of similarity among seep sites supports the idea that there are no specific infauna that can be used as indicators of seepage throughout the northern Gulf of Mexico, at least at higher taxonomic levels.  相似文献   

Long-Term Evaluation of Mulch Biowall Performance to Treat Chlorinated Solvents     

Kenneth L. Walker Jr.  Travis M. McGuire  David T. Adamson  R. Hunter Anderson 《Ground Water Monitoring & Remediation》2020,40(1):35-46

Permeable reactive barriers (PRBs), such as mulch biowalls, have been installed at numerous groundwater cleanup sites, and laboratory and field studies have demonstrated biotic and abiotic processes that degrade chlorinated volatile organic compounds (CVOCs) in groundwater passing through these engineered remedies. However, the longevity of mulch biowalls remains a fundamental research question. Soil and groundwater sampling at seven mulch biowalls at Altus Air Force Base (AFB) approximately 10 years after installation demonstrated the ongoing degradation of CVOCs. Trichloroethene was not detected in five of seven groundwater samples collected from the biowall despite upgradient detections above federal drinking water standards. Microbial sampling established the presence of key dechlorinating bacteria and the abundance of genes encoding specific enzymes for degradation, high methane concentrations, low sulfate concentrations, and negative oxidation-reduction potential, all indicative of highly reducing conditions within the biowalls and favorable conditions for CVOC destruction via microbial reductive dechlorination. High cellulose content (>79%) of the mulch, elevated total organic carbon (TOC) content in groundwater, and elevated potentially bioavailable organic carbon (PBOC) measurements in soil samples further supports an ongoing, long-lived source of carbon. These results demonstrate the ongoing and long-term efficacy of the mulch biowalls at Altus AFB. In addition, concentrations of bacteria, TOC, PBOC, and other geochemical parameters suggest a modest impact of the biowalls downgradient. The continued presence of CVOCs downgradient may be attributable to back diffusion from low-permeability shale. However, the biowalls continue to provide benefits by removing CVOCs in groundwater, thus reducing further CVOC loading to the downgradient, low-permeability strata.  相似文献   

A benchmark comparison of numerical methods for infinite Prandtl number thermal convection in two-dimensional Cartesian geometry     

B. J. Travis  C. Anderson  J. Baumgardner  C. W. Gable  B. H. Hager  R. J. O'Connell 《地球物理与天体物理流体动力学》2013,107(3-4):137-160

Abstract

A comparison is made between seven different numerical methods for calculating two-dimensional thermal convection in an infinite Prandtl number fluid. Among the seven methods are finite difference and finite element techniques that have been used to model thermal convection in the Earth's mantle. We evaluate the performance of each method using a suite of four benchmark problems, ranging from steady-state convection to intrinsically time-dependent convection with recurring thermal boundary layer instabilities. These results can be used to determine the accuracy of other computational methods, and to assist in the development of new ones.  相似文献   

Limits on the 2.2-μm contrast ratio of the close-orbiting planet HD 189733b     

J. R. Barnes  Travis S. Barman  L. Prato  D. Segransan  H. R. A. Jones  C. J. Leigh  A. Collier Cameron  D. J. Pinfield 《Monthly notices of the Royal Astronomical Society》2007,382(1):473-480

We obtained 238 spectra of the close-orbiting extrasolar giant planet HD 189733b with resolution   R ∼ 15 000  during one night of observations with the Near-Infrared High-Resolution Spectrograph (NIRSPEC), at the Keck II Telescope. We have searched for planetary absorption signatures in the  2.0–2.4 μm  region where H₂O and CO are expected to be the dominant atmospheric opacities. We employ a phase-dependent orbital model and tomographic techniques to search for the planetary absorption signatures in the combined stellar and planetary spectra. Because potential absorption signatures are hidden in the noise of each single exposure, we use a model list of lines to apply a spectral deconvolution. The resulting mean profile possesses a signal-to-noise ratio (S/N) that is 20 times greater than that found in individual lines. Our spectral time series thus yields spectral signatures with a mean S/N = 2720. We are unable to detect a planetary signature at a contrast ratio of  log₁₀( F _p/ F _*) =−3.40  , with 63.8 per cent confidence. Our findings are not consistent with model predictions which nevertheless give a good fit to mid-infrared observations of HD 189733b. The 1σ result is a factor of 1.7 times less than the predicted 2.185-μm planet/star flux ratio of  log₁₀( F _p/ F _*) ∼−3.16  .  相似文献   

Sensitivity of the regional climate model RegCM4.2 to planetary boundary layer parameterisation     

Ivan Güttler  Čedo Branković  Travis A. O’Brien  Erika Coppola  Branko Grisogono  Filippo Giorgi 《Climate Dynamics》2014,43(7-8):1753-1772

This study investigates the performance of two planetary boundary layer (PBL) parameterisations in the regional climate model RegCM4.2 with specific focus on the recently implemented prognostic turbulent kinetic energy parameterisation scheme: the University of Washington (UW) scheme. When compared with the default Holtslag scheme, the UW scheme, in the 10-year experiments over the European domain, shows a substantial cooling. It reduces winter warm bias over the north-eastern Europe by 2 °C and reduces summer warm bias over central Europe by 3 °C. A part of the detected cooling is ascribed to a general reduction in lower tropospheric eddy heat diffusivity with the UW scheme. While differences in temperature tendency due to PBL schemes are mostly localized to the lower troposphere, the schemes show a much higher diversity in how vertical turbulent mixing of the water vapour mixing ratio is governed. Differences in the water vapour mixing ratio tendency due to the PBL scheme are present almost throughout the troposphere. However, they alone cannot explain the overall water vapour mixing ratio profiles, suggesting strong interaction between the PBL and other model parameterisations. An additional 18-member ensemble with the UW scheme is made, where two formulations of the master turbulent length scale in unstable conditions are tested and unconstrained parameters associated with (a) the evaporative enhancement of the cloud-top entrainment and (b) the formulation of the master turbulent length scale in stable conditions are systematically perturbed. These experiments suggest that the master turbulent length scale in the UW scheme could be further refined in the current implementation in the RegCM model. It was also found that the UW scheme is less sensitive to the variations of the other two selected unconstrained parameters, supporting the choice of these parameters in the default formulation of the UW scheme.  相似文献   

Mapping of Io's thermal radiation by the Galileo photopolarimeter-radiometer (PPR) instrument   总被引：1，自引：0，他引：1  

J.A. Rathbun  J.R. Spencer  T.Z. Martin  L.D. Travis 《Icarus》2004,169(1):127-139

Between 1999 and 2002, the Galileo spacecraft made 6 close flybys of Io during which many observations of Io's thermal radiation were made with the photopolarimeter-radiometer (PPR). While the NIMS instrument could measure thermal emission from hot spots with T>200 K, PPR was the only Galileo instrument capable of mapping the lower temperatures of older, cooling lava flows, and the passive background. We tabulate all data taken by PPR of Io during these flybys and describe some scientific highlights revealed by the data. The data include almost complete coverage of Io at better than 250 km resolution, with extensive regional coverage at higher resolutions. We found a modest poleward drop in nighttime background temperatures and evidence of thermal inertia variations across the surface. Comparison of high spatial resolution temperature measurements with observed daytime SO₂ gas pressures on Io provides evidence for local cold trapping of SO₂ frost on scales smaller than the 60 km resolution of the PPR data. We also calculated the power output from several hot spots and estimated total global heat flow to be about 2.0-2.6 W m⁻². The low-latitude diurnal temperature variations for the regions between obvious hot spots are well matched by a laterally-inhomogeneous thermal model with less than 1 W m⁻² endogenic heat flow.  相似文献   

Lessons for asteroseismology from white dwarf stars     

Travis S. Metcalfe 《Journal of Astrophysics and Astronomy》2005,26(2-3):273-281

The interpretation of pulsation data for sun-like stars is currently facing challenges quite similar to those faced by white dwarf modelers ten years ago. The observational requirements for uninterrupted long-term monitoring are beginning to be satisfied by successful multi-site campaigns and dedicated satellite missions. But exploration of the most important physical parameters in theoretical models has been fairly limited, making it difficult to establish a detailed best-fit model for a particular set of oscillation frequencies. I review the past development and the current state of white dwarf asteroseismology, with an emphasis on what this can tell us about the road to success for asteroseismology of other types of stars.  相似文献   

Budgeting for climate change: obstacles and opportunities at the US state level     

Elisabeth A. Gilmore  Travis St.Clair 《Climate Policy》2018,18(6):729-741

State governments in the United States are well placed to identify opportunities for mitigation and the needs for adaptation to climate change. However, the cost of these efforts can have important implications for budgets that already face pressures from diverse areas such as unfunded pensions and growing health care costs. In this work, the current level of spending on climate-related activities at the state level are evaluated and policy recommendations are developed to improve financial management practices as they relate to climate risk. An examination of state budgets reveals that climate mitigation and adaptation activities represent less than 1% of spending in most states. The data collection highlights the obstacles to collecting accurate spending data and the lack of budgetary and accounting procedures in place. More importantly, the difficulty in benchmarking these activities poses challenges for the analysis of state-level policies as well as planning and modelling future climate-related spending. Other policy contexts, including public pensions and infrastructure, can provide guidance on budgetary and accounting tools that may help states prepare for and more efficiently manage climate-related expenditures.

Key policy insights

• Climate change mitigation and adaptation will require substantial investments across many levels of government on a wide range of activities.

• Currently, US states are not clearly demarcating climate expenditures, hindering the identification of climate-related budgetary risks.

• In the absence of guidelines, these longer term fiscal outlays may remain chronically underfunded in favour of more near-term spending priorities.

• Establishing appropriate financial management and data collection practices is important for more sophisticated cost-effectiveness and policy analyses.

  相似文献   

The Citation Impact of Digital Preprint Archives for Solar Physics Papers   总被引：1，自引：0，他引：1  

Travis S. Metcalfe 《Solar physics》2006,239(1-2):549-553

Papers that are posted to a digital preprint archive are typically cited twice as often as papers that are not posted. This has been demonstrated for papers published in a wide variety of journals, and in many different subfields of astronomy. Most astronomers now use the arXiv.org server (astro-ph) to distribute preprints, but the solar physics community has an independent archive hosted at Montana State University. For several samples of solar physics papers published in 2003, I quantify the boost in citation rates for preprints posted to each of these servers. I show that papers on the MSU archive typically have citation rates 1.7 times higher than the average of similar papers that are not posted as preprints, while those posted to astro-ph get 2.6 times the average. A comparable boost is found for papers published in conference proceedings, suggesting that the higher citation rates are not the result of self-selection of above-average papers. Editors’ Note: This paper lies outside the normal purview of Solar Physics papers, however the editors feel that the content is of sufficient importance for all Solar Physics authors and readers to merit its publication.  相似文献