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1.
Screening bioactive natural products from bacteria is a determinative step in the drug discovery programs. The present study aim to isolate actinobacteria from the Oman Sea sediments for determining the effects of different culture media and treatments on the yield of the isolation process, and measure the DPPH radical scavenging and Artemia cytotoxic activity of culture extracts of the actinobacterial isolates. A total of 290 actinobacterial isolates were collected from 14 sediment samples. Heat treatment(40.68%) and M_4 medium(29.31%) exhibited the maximum isolation rates of actinobacteria. Streptomyces isolates were dominantly distributed in all of the investigated stations according to 16 S rRNA gene sequencing. The distribution pattern of Streptomyces followed a depth-dependent frequency trend, whereas the members of rare genera including Micromonospora, Nocardia Actinoplanes, Nocardiopsis, Saccharopolyspora and Crossiella were distributed in deeper stations. Approximately,25% of the examined isolates could scavenge 90% of 10~(–4) mol/L DPPH solutions at 1 250 μg/mL final concentration of their ethyl acetate culture extracts. Furthermore, the most potent extracts could scavenge DPPH radicals with IC50 ranges from 356.8 to 566.4 μg/mL. Brine shrimp cytotoxicity tests showed that 38.88% of the examined culture extracts exhibited LC_(50) lower than 1 000 μg/mL against the Artemia cells. Moreover, the most potent culture extracts exhibited LC_(50) range from 335.4 to 534.4 μg/mL. Phylogenetic analysis by 16 S rRNA gene sequence revealed that the OS 005, OS 263 and OS 157 closely related to Streptomyces djakartensis, Streptomyces olivaceus and Nocardiopsis dassonvillei respectively. These results suggested the widespread distribution of the antioxidant and cytotoxic producing actinobacteria in the Oman Sea sediments, which could be considered as promising candidates for the discovery of microbial bioactive compounds.  相似文献   
2.
This paper presents a step-by-step procedure using the three-dimensional boundary element approach to study the behavior of semi-circular canyons under seismic shear waves. The boundary element code TDASC allows utilization for various canyon geometries, evaluation of concurrent seismic waves and calculation of the ground motions on canyons due to an excitation at any arbitrary point of the incident field. Considering the widening ratio of the canyon(including prismatic, semi-prismatic and non-prismatic canyons), wave characteristics(wavelength, dimensionless period, direction) and maximum amplification pattern, the solution was applied to carry out a series of parametric studies. It was shown that canyon form can significantly affect the displacement amplification, especially at the points located on its edges. By increasing the wave dimensionless frequency(η 1), the amplification pattern becomes more complex. On the basis of the results from a variety of considered cases, a new expression has been presented for the limiting wavelength beyond which the widening of the canyon will not have a major effect on the displacement amplification. To verify the reliability of the proposed approach, the obtained results, expressed in terms of displacement amplitude, were compared with those from the available published literature and a reasonably good agreement was observed.  相似文献   
3.
In this research the tail equivalent linearization method(TELM) has been extended to study structures with degrading materials. The responses of such structures to excitations are non-stationary, even if the excitations are stationary. Non-stationary behavior of the system cannot be considered by conventional TELM. Applying the conventional TELM, the only distinction in the design point excitation for two stationary excitations with different durations is in the addition of a zero value part at the beginning of the design point of the longer excitation. This means that the failure probability is the same for the non-stationary systems under excitations with different durations. Therefore, this solution cannot be correct. In this study, in using TELM for systems with degrading materials, hysteretic energy is replaced by average hysteretic energy, calculated by averaging the obtained hysteretic energy of the structure subjected to a few random sample load realization. In this way, the degradation parameters under design point coincide with those under sample load realizations. Since the average of the hysteretic energy is converges very fast, the modified TELM only requires about tens to hundreds solutions of the response in addition to the ordinary calculations of conventional TELM.  相似文献   
4.
Though it is well known that vegetation affects the water balance of soils through canopy interception and evapotranspiration, its hydrological contribution to soil hydrology and stability is yet to be fully quantified. To improve understanding of this hydrological process, soil water dynamics have been monitored at three adjacent hillslopes with different vegetation covers (deciduous tree cover, coniferous tree cover, and grass cover), for nine months from December 2014 to September 2015. The monitored soil moisture values were translated into soil matric suction (SMS) values to facilitate the analysis of hillslope stability. Our observations showed significant seasonal variations in SMS for each vegetation cover condition. However, a significant difference between different vegetation covers was only evident during the winter season where the mean SMS under coniferous tree cover (83.6 kPa) was significantly greater than that under grass cover (41 kPa). The hydrological reinforcing contribution due to matric suction was highest for the hillslope with coniferous tree cover, while the hillslope with deciduous tree cover was second and the hillslope with grass cover was third. The greatest contributions for all cover types were during the summer season. During the winter season, the wettest period of the monitoring study, the additional hydrological reinforcing contributions provided by the deciduous tree cover (1.5 to 6.5 kPa) or the grass cover (0.9 to 5.4 kPa) were insufficient to avoid potential slope failure conditions. However, the additional hydrological reinforcing contribution from the coniferous tree cover (5.8 to 10.4 kPa) was sufficient to provide potentially stable hillslope conditions during the winter season. Our study clearly suggests that during the winter season the hydrological effects from both deciduous tree and grass covers are insufficient to promote slope stability, while the hydrological reinforcing effects from the coniferous tree cover are sufficient even during the winter season. Copyright © 2018 John Wiley & Sons, Ltd.  相似文献   
5.
An experimental campaign was set up to quantify the contribution of evapotranspiration fluxes on hillslope hydrology and stability for different forest vegetation cover types. Three adjacent hillslopes, respectively, covered by hardwood, softwood, and grass were instrumented with nine access tubes each to monitor soil water dynamics at the three depths of 30, 60, and 100 cm, using a PR2/6 profile probe (Delta‐T Devices Ltd) for about 6 months including wet periods. Soil was drier under softwood and wetter under grass at all the three depths during most of the monitoring period. Matric suction derived via the soil moisture measurements was more responsive to changes in the atmospheric conditions and also recovered faster at the 30 cm depth. Results showed no significant differences between mean matric suction under hardwood (101.6 kPa) with that under either softwood or grass cover. However, a significant difference was found between mean matric suction under softwood (137.5 kPa) and grass (84.3 kPa). Results revealed that, during the wettest period, the hydrological effects from all three vegetation covers were substantial at the 30 cm depth, whereas the contribution from grass cover at 60 cm (2.0 kPa) and 100 cm (1.1 kPa) depths and from hardwood trees at 100 cm depth (1.2 kPa) was negligible. It is surmised that potential instability would have occurred at these larger depths along hillslopes where shallow hillslope failures are most likely to occur in the region. The hydrological effects from softwood trees, 8.1 and 3.9 kPa, were significant as the corresponding factor of safety values showed stable conditions at both depths of 60 and 100 cm, respectively. Therefore, the considerable hydrological reinforcing effects from softwood trees to the 100 cm depth suggest that a hillslope stability analysis would show that hillslopes with softwood trees will be stable even during the wet season.  相似文献   
6.
An accurate estimate of the groundwater inflow to a tunnel is one of the most challenging but essential tasks in tunnel design and construction. Most of the numerical or analytical solutions that have been developed ignore tunnel seepage conditions, material properties and hydraulic-head changes along the tunnel route during the excavation process, leading to inaccurate prediction of inflow rates. A method is introduced that uses MODFLOW code of GMS software to predict inflow rate as the tunnel boring machine (TBM) gradually advances. In this method, the tunnel boundary condition is conceptualized and defined using Drain package, which is simulated by dividing the drilling process into a series of successive intervals based on the tunnel excavation rates. In addition, the drain elevations are specified as the respective tunnel elevations, and the conductance parameters are assigned to intervals, depending on the TBM type and the tunnel seepage condition. The Qomroud water conveyance tunnel, located in Lorestan province of Iran, is 36 km in length. Since the Qomroud tunnel involved groundwater inrush during excavating, it is considered as a good case study to evaluate the presented method. The groundwater inflow to this tunnel during the TBM advance is simulated using the proposed method and the predicted rates are compared with observed rates. The results show that the presented method can satisfactorily predict the inflow rates as the TBM advances.  相似文献   
7.
Bulletin of Earthquake Engineering - Automated Multi-Depth Shuttle Warehouses (AMSWs) are compact storage systems that provide a large surface occupation and therefore maximum storage density....  相似文献   
8.
9.
This study aimed to identify indicator species and explore the most important environmental and management variables contributing to vegetation distribution in a hilly upper dam landscape in Zagros Mountain chain, Iran. A stratified random sampling method was used to collect topographic, edaphic, management and vegetation data. The density and cover percentage of perennial species were measured quantitatively. Indicator species were identified using the two-way indicator species analysis. Besides calculating physiognomic factors in sample sites, 24 soil samples were collected from 0 to 30 cm of soil depth and analyzed in terms of gravel percentage, texture, saturation moisture, organic matter, pH and electrical conductivity in saturation extract, lime percentage, soluble calcium and magnesium, available phosphorus, Cation Exchange Capacity(CEC) and soluble sodium and potassium. Multivariate techniques including Canonical Correspondence Analysis and Multi-Dimensional Scaling were used to explore the relationships of species with environmental and management variables. Seven plants were identified as indicator species due to being significantly correlated with management(grazing or non-grazing) and edaphic variables such as CEC, soil texture, pH, CaCO_3 percentage and physiographic variable including slope, elevation, and convex and concave formations(p 0.05). Overall, overgrazing and its subsequent effects on soil characteristics, loss of vegetation cover and trampling were found as the major causes of deterioration. Sustainable and integrated management practices such as the implementation of appropriate grazing systems were suggested to enhance soil quality and reduce the accelerated erosion in upper dam zones.  相似文献   
10.
Several proposals are explored for the hazard and intensity measure (IM) consistent selection of bidirectional ground motions to assess the performance of 3D structural models. Recent studies have shown the necessity of selecting records that thoroughly represent the seismicity at the site of interest, as well as the usefulness of efficient IMs capable of estimating the response of buildings with low scatter. However, the advances realized are mostly geared towards the structural analysis of 2D models. Few are the combined record, and IM selection approaches suggested expressly for nonlinear dynamic analysis of 3D structural models, especially when plan asymmetry and torsion sensitivity come into play. Conditional spectrum selection is leveraged and expanded here to offer a suite of approaches based on both scalar and vector IMs that convey information from two orthogonal horizontal components of the ground motion. Applications on multiple 3D building models highlight the importance of (a) employing the same IM for both record selection and response assessment and (b) maintaining hazard consistency in both horizontal components, when using either a scalar or a vector IM. All tested approaches that respect these conditions can be viable, yet the one based on the geometric mean of multiple spectral ordinates from both components over a period range seems to hold the most promise for general use.  相似文献   
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