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肠道和肝胰腺是中华绒螯蟹(Eriocheir sinensis)重要的消化器官,本文运用组织学技术测量了中肠和后肠长度,并对肠道长与体长进行相关性分析。应用免疫组化技术对5-HT和5-HT受体2进行组织定位和计数。结果表明:(1)中华绒螯蟹的肠道长与体长接近,两者之间呈极显著正相关(P0.01)。后肠长度约为中肠的2倍;(2)5-HT和5-HT受体2在中肠和后肠中的组织定位和数量相似,均分布于黏膜上皮细胞间和肌层与外膜交界处,亦见于后肠的固有膜和黏膜下层与肌层交界处,在肠球外膜中也有大量分布;(3)5-HT和5-HT受体2阳性物质在肝胰腺的四种细胞的胞核中均有分布,在F细胞的胞质中也有分布。5-HT和5-HT受体2在中华绒螯蟹肠道和肝胰腺组织中的分布规律一致,表明中华绒螯蟹消化道5-HT发挥生理功能可能与5-HT受体2有关。此外,5-HT和5-HT受体2在后肠近肌细胞分布的特点可能与肠道收缩有关。后肠在中华绒螯蟹消化生理中的作用应引起重视。 相似文献
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To determine the existence of p-nitrophenol (PNP)-lowering bacteria in intestine of Japanese coastal fish, the gastro-intestinal contents were incubated in Brain Heart Infusion (BHI) broth and minimal medium (MM) broths containing 1 mmol/L PNP at 30 °C for 7 days. Among 26 samples of 19 fish species, 17 samples showed a decrease in PNP of 0.5-0.8 mmol/L in BHI broth, but no decrease was shown in MM broth. Eighteen PNP-lowering bacterial strains were isolated from four fishes. All of the strains were identified as Lactococcus lactis subsp. lactis. Three L. lactis strains JS1-3 isolated from Japanese seabass Lateolabrax japonicus showed the highest PNP-lowering activity (0.44 mmol/L). Optimum temperature and pH for the growth and PNP decreasing corresponded with the marine environment. These results suggested that marine fishes have PNP decreasing bacteria in their intestine. These bacteria might protect host fish from toxicities of PNP and PNP related compounds. 相似文献
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This study on the cytological changes of the body wall ,aspiratory intestine and crissal bursa in Urechis unicincuts by light microscope(LM) and transmission electron microscope(TEM) showed that the difference between the body wall and the natural environment rich in H2S was not obvious,that the wall color of the aspiratory intestine in H2S rich environment changed from normal semitransparency to dark brown.that its epithelia were disassembled and the electron density of its cytoplasm matrix was lower;and that in H2S rich environment many basoplhilic granules occurred in the epithelia of the crissal bursa.Granules with single membrane and myelinefingure were found with TEM. 相似文献
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Results are presented from numerical simulations of the incidence of a spatially-compact beam of internal waves on a shear layer containing a critical level. It is found that a significant fraction of the energy of the incident beam is transmitted across the critical level when the packet is narrow in physical space and when the energy density of the packet is not too high so that nonlinear effects are weak. As either the packet width or its energy density increases, the presence of a critical level renders the shear layer increasingly opaque to the transmission of energy. The familiar exponentially small value of the transmission coefficient is recovered as the spatial width of the packet exceeds (roughly) eight to ten wave lengths. 相似文献
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Dynamics of the three-dimensional structure of the wind-driven Deacon cell in a β-plane channel are discussed in a homogeneous model in the presence of a sufficiently high ridge. The emphasis is on the water mass balance: how the northward surface Ekman drift is returned. It is demonstrated that a sufficiently high ridge can break up the geostrophic constraint and a net geostrophic volume flux across the open latitude band is allowed. It is found that: (1) the Deacon cell is a fundamentally three-dimensional structure, (2) wind forcing can drive an inter-basin water mass exchange in the Southern Ocean, and (3) zonal through-channel transport in the circumpolar ocean varies at different longitudes. 相似文献
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While the important role of land–ocean contrast (LOC) in the mean atmospheric circulation is well-known, an intriguing question remains as to whether LOC can also significantly influence the anomaly circulation. This question is particularly important in the tropics, where a large part of the variabilities is known to be due to convective internal dynamics, which in turn can be significantly affected by LOC. In the present work, we investigate this question using a model of convectively driven anomaly circulation in the tropics. Emphasizing the lower tropospheric flow, we adopt a model describing the horizontal dynamics of the first baroclinic mode on an equatorial β-plane, in the presence of moist feedbacks. We introduce LOC in both latitudinal and longitudinal directions. Land surface dryness is taken into account in the moisture budget through the control of evaporative flux. A constant non-latent heating term is used over land in order to represent radiative and sensible heating effects. First, a control run is performed, numerically, without any LOC using random initial perturbations. The gravest mode that emerges from the control run, which is a wave-2 feature with a period around 20 days, is then used as the initial field for the model runs with LOC. The results show that LOC can significantly influence even the tropical variabilities. A latitudinal LOC, with a land mass in the northern hemisphere (north of 10°N), tends to shift the region of maximum precipitation slightly north of the equator with accompanying steeper gradients near the land–ocean boundary. The implications of this result for our understanding of Asian summer monsoon conditions are discussed. When LOC is only in the longitudinal direction, the dominant wave structure that emerges from the model run has wavenumber one and a period of about 35 days, very similar to the observed 30–60 day oscillation. In our final experiment, which is nearer to the realistic land–ocean configuration in the tropics, it is found that both latitudinal and longitudinal effects of LOC are important aspects of the tropical anomaly circulation. It is suggested that some of the patterns in the precipitation distribution, observed in the tropics and simulated using general circulation models are results of convectively induced internal dynamics, modulated by LOC. 相似文献
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温度对小黄鱼体内抗氧化酶及消化酶活性的影响 总被引：1，自引：0，他引：1
为了深入了解不同温度条件下小黄鱼的应激反应,为小黄鱼的人工养殖提供参考。本次研究以人工繁殖的4.5月龄小黄鱼为研究对象,采用不同水温（27℃、29℃、31℃、32℃、33℃）进行养殖,以此确定小黄鱼的高温致死温度,并检测不同养殖水温条件下小黄鱼肝脏和肠道中抗氧化酶（超氧化物歧化酶SOD、过氧化氢酶CAT）和消化酶（脂肪酶LPS、淀粉酶AMS）的活性,比较小黄鱼不同组织中4种酶活性的变化情况。结果显示,32℃的养殖水温为小黄鱼的初始致死温度;养殖温度对小黄鱼肝脏和肠道中几种酶的活性均存在显著影响（P<0.05）:抗氧化酶和消化酶活性均随着养殖水温的升高呈现先升高后下降的趋势（除了肠道中的CAT）。另外,肝脏中抗氧化酶活性强于消化酶,而肠道中则相反,表明肝脏侧重于机体免疫反应,而肠道更侧重于食物消化。通过二次项回归方程拟合了温度与酶活性的回归方程,只有肝脏和肠道中的LPS活性与温度的回归方程有意义,决定系数r2分别为0.89和0.93（在0.85以上）。此外,肝脏中LPS活性与实验鱼体质量呈极显著正相关关系（P<0.01）,研究结果为小黄鱼专用配合饲料的研发提供了重要参考。研究还得出,养殖水温29℃和31℃时,进行小黄鱼养殖可取得较好效果,研究结果可为小黄鱼快速养成提供指导。 相似文献
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ＩＮＴＲＯＤＵＣＴＩＯＮＵｒｅｃｈｉｓａｎｉｍａｌｓａｒｅｏｆｔｅｎｐｒｅｓｅｎｔｉｎＨ2 Ｓｒｉｃｈｓｅａａｒｅａｗｈｅｒｅｍｏｓｔａｎｉｍａｌｓｃａｎｎｏｔｌｉｖｅ .ＩｔｃａｎｔｏｌｅｒａｔｅｔｈｅｓｔｒｏｎｇｔｏｘｉｃｉｔｙｏｆＨ2 Ｓ .ＭａｎｙｓｃｈｏｌａｒｓｗｅｒｅｉｎｔｅｒｅｓｔｅｄｉｎｔｈｅｔｏｌｅｒａｔｉｎｇｍｅｃｈａｎｉｓｍｏｆＨ2 ＳｉｎｔｈｅＵｒｅｃｈｉｓａｎｉｍａｌ (ＰｏｗｅｌｌａｎｄＡｒｐ ,1 989;Ａｒｐｅｔａｌ.,1 992 ) .Ａｒｐｅｔａｌ.(1 992 )ｉｎｄｉｃａｔｅｄｔｈａｔｔｈｅ… 相似文献
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【目的】研究复方中草药对虹鳟(Oncorhynchus mykiss)肝脏免疫功能及肠道免疫相关基因的影响。【方法】将红枣提取物、山药提取物和黄芪提取物按照质量比1∶1∶1混合,分别按质量分数0(对照)、0.3%、0.6%和1.2%添加到虹鳟基础饲料中,饲喂体长7.6~8.9 cm的虹鳟幼鱼56 d。用副溶血弧菌(Vibrio parahemolyticus)对幼鱼进行攻毒试验(5.48×106 cfu/尾),4 d后,取其肝脏和肠道,测定肝脏的生理生化指标,用半定量PCR及荧光定量PCR测定肠道免疫相关基因的表达。【结果】与对照组比较,肝脏的总抗氧化能力(T-AOC)显著升高(P<0.05),溶菌酶(LZM)水平极显著升高(P<0.01),酸性磷酸酶(ACP)、过氧化氢酶(CAT)活性显著降低(P<0.05),碱性磷酸酶(ALP)活性极显著降低(P<0.01),0.6%剂量组的丙二醛(MDA)极显著降低(P<0.01),而SOD无显著差异(P>0.05);各复方中草药组肠道的肿瘤坏死因子(TNF-α)及1.2%剂量组转化生长因子-β(TGF-β)转录水平极显著降低(P<0.01),0.3%和0.6%剂量组的补体3(C3)转录水平显著升高(P<0.05)。【结论】复方中草药(红枣+山药+黄芪)可提高感染副溶血弧菌的虹鳟非特异性免疫功能。 相似文献