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1.
Polypeptide from Chlamys farreri inhibits UVB-induced apoptosis of HaCaT cells via iNOS/NO and HSP90
Polypeptide from Chlamys farreri(PCF) is a novel marine bioactive product that was isolated from the gonochoric Chinese scallop Chlamys farreri,and was found to be an effective antioxidant in our recent studies.In this study,we investigated the effect of PCF on ultraviolet B(UVB)-induced apoptosis of HaCaT cells and the intracellular signaling pathways involved.Pretreatment with the inducible nitric oxide synthase(iNOS) inhibitor S-methylisothiourea sulfate inhibited UVB-induced apoptosis,indicating that iN... 相似文献
2.
This study investigated the molecular mechanism of anti-tumor effect of fascaplysin, a nitrogenous red pigment firstly isolated from a marine sponge. Microarray analysis show that the TNF and TNF receptor superfamily in human umbilical vein endothelial cells (HUVEC) and human hepatocarcinoma cells (BEL-7402) were significantly regulated by fascaplysin. Western Blot results reveal that fascaplysin increased the expression of cleaved caspase-9, active caspase-3, and decreased the level of procaspase-8 and Bid. Flow cytometry and cytotoxicity tests indicate that fascaplysin sensitized cells to tumor necrosis-related apoptosis-inducing ligand-(TRAIL) induced apoptosis, which was markedly blocked by TRAIL R2/Fc chimera, a dominant negative form of TRAIL receptor DR5. Therefore, our results demonstrate that fascaplysin promotes apoptosis through the activation of TRAIL signaling pathway by upregulating DR5 expression. 相似文献
3.
1 INTRODUCTION Active oxygen free radicals can damage cell structure, even kill the cells, causing aging and cer- tain serious diseases (Bors et al., 1989). Usually, the production and scavenging of active oxygen free radicals are in balance in healthy hu… 相似文献
4.
Zhang Yujiang Zhan Songmei Cao Pengli Liu Ning Chen Xuehong Wang Yuejun Wang Chunbo 《中国海洋湖沼学报》2005,23(3):357-362
To investigate the effect of polypeptide fromChlamys farreri (PCF) on NHDFin vitro, we modeled oxidative damage on normal human dermal fibroblasts (NHDF) exposed to ultraviolet B (UVB). In this study, 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium
bromide (MTT) and lactate dehydrogenase (LDH) were tested to measure cell viability. Enzymes including superoxide dismutase
(SOD), glutathione peroxidase (GSH-PX), catalase (CAT) and xanthine oxidase (XOD) were determined biochemically. Total antioxidative
capacity (T-AOC) and anti-superoxide anion capacity (A-SAC) were also determined. Ultrastructure of fibroblasts was observed
under transmission electron microscope. The results showed that: UVB (1.176×10−4 J/cm2) suppressed the growth of fibroblasts and the introduction of PCF (0.25%–1%) before UVB reduced the suppression in a concentration-dependent
manner. PCF could enhance the activities of SOD, GSH-PX and T-AOC as well as A-SAC. Also PCF could inhibit XOD activity, while
it did not affect CAT activity. Ultrastructure of fibroblasts were damaged after UVB irradiation, concentration-dependent
PCF reduced the destructive effect of UVB on cells. These results indicated that PCF can protect human dermal fibroblasts
from being harmed by UVB irradiation via its antioxidant proerty.
This work was supported by the National Natural Science Foundation of China (No. 39970638) and the Science and Technology
Bureau of Qingdao (No. 2001-28-50). 相似文献
5.
Polypeptide fromChlamys farreri (PCF), a topical polypeptide isolated fromChlamys farreri, was used in this experiment aimed to investigate the photoprotective effect of PCF against chronic skin damage induced by
ultraviolet A (UVA) and ultraviolet B (UVB) radiation. The chronic ultraviolet-irradiated guinea pig model was established,
and visible changes in the skin including wrinkling, sagging and erythema were observed. Malondialdehyde (MDA) and antioxidant
enzymes including superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) in the dorsal skin were determined using
biochemical methods. The results showed: (1) PCF (5 % and 20%) could greatly protect the dorsal skin of guinea pig against
wrinkling, sagging and erythema induced by UV radiation in a concentration-dependent manner. (2) PCF could reduce MDA formation
in the dorsal skin caused by UV irradiation, while increasing the activities of SOD and GSH-px. (3) The differences among
the PCF groups and UV model group were significant (P<0.05,P<0.01). These results indicated that topical application, of PCF provided broad solar UV spectrum photoprotection; and that
the antioxidant property of PCF might play a role in photoprotection.
Project No. 39970638 supported by the NSFC, and the Science and Technology Bureau of Qingdao (No. 2001-28-50). 相似文献
6.
1 INTRODUCTION Long-term exposure of the skin to ultraviolet (UV) radiation results in degenerative processes involved in photoaging and photocarcinogenesis (Differy, 1987). As UVC is absorbed entirely by the stratospheric ozone layer and does not reach the earth抯 surface, sunlight is composed of UVA (320 nm 400 nm) and UVB (290 nm 320 nm). Human skin is exposed constantly to both UVA and UVB radiation, it is, therefore, important to protect the skin against UVA and UVB-induce… 相似文献
7.
Protective Effect of Topically Applied Polypeptide from Chlamys farreri Against Ultraviolet Radiation-Induced Chronic Skin Damage in Guinea Pig 总被引：2，自引：0，他引：2
INTRODUCTIONSolarultraviolet(UV)radiationisknowntobeamajoretiologicalriskofphotoaging.AsUVC(2 0 0nm -2 90nm)isabsorbedentirelybythestratosphericozonelayer,sunlightontheearthsur faceiscomposedofUVA (3 2 0nm -4 0 0nm)andUVB (2 90nm -3 2 0nm)only.UVAradiationenergyaccountsformorethan 90 %ofsolarUV ;andpenetratesthebasallayerofepidermismoreefficientlythanUVB (Brulsetal.,1984) .However,atsimilardosages,UVBismuchmoremuta genicthanUVA (Drobetskyetal.,1995) .Furthermore ,humanskinisexp… 相似文献
8.
We examined the effects of polypeptide fromChlamys farreri (PCF) on the amount of hydroxyproline in guinea pig skin irradiated by chronic ultraviolet A (UVA) and ultraviolet B (UVB)
radiation. PCF was applied locally before repeated exposure of guinea pig to UVA and UVB. The contents of hydroxyproline and
other amino acids in guinea pig skin were determined by automatic amino acid analyzer. Our results showed that: (1) long-time
UVA and UVB radiation can reduce dramatically the amounts of hydroxyproline, aspartic acid, threonine, glycine, phenylalanine
and lysine in guinea pig skin in comparison with the control group (P<0.05); (2) Compared with model group, pre-treatment with 5% and 20% PCF prior to UVA and UVB radiation can inhibit the decline
of amino acids content in guinea pig skin in a dose-dependent manner (P<0.05). As the decrease of hydroxyproline, glycine and lysine contents in the skin directly reflexes type I collagen degeneration,
our results indicated that the chronic application of PCF can protect skin type I collagen against UV radiation, and thus
protect skin from photoaging.
Project No. 39970638 supported by the NSFC, and also supported by the Science and Technology Bureau of Qingdao (No: 2001-28-50). 相似文献
9.
HUANG Fei WANG Hong QIAN Junping WANG Guofu 《中国海洋大学学报(英文版)》2006,5(3):207-212
Based on the atmospheric horizontal visibility data from forty-seven observational stations along the eastern coast of China near the Taiwan Strait and simultaneous NOAA/AVHRR multichannel satellite data during January 2001 to December 2002, the spectral characters associated with visibility were investigated. Successful retrieval of visibility from multichannel NOAA/AVHRR data was performed using the principal component regression （PCR） method. A sample of retrieved visibility distribution was discussed with a sea fog process. The correlation coefficient between the observed and retrieved visibility was about 0.82, which is far higher than the 99.9% confidence level by statistical test. The rate of successful retrieval is 94.98% of the 458 cases during 2001 2002. The error distribution showed that high visibilities were usually under-estimated and low visibilities were over-estimated and the relative error between the observed and retrieved visibilities was about 21.4%. 相似文献
10.
GPS/VLBI/SLR/InSAR组合在地球动力学研究中的应用 总被引：8，自引：4，他引：4
详细探讨了InSAR技术应用于地球动力学研究的巨大潜力和优势；讨论了InSAR与GPS等其它空间技术组合的可能性。提出了GPS／VLBI／SLR／InSAR组合的新设想，并详细讨论了该方法在地球动力学、现代地壳运动、地球体积和形状变化研究中的应用。在具体组合应用中，针对地壳不同形变特征给出了3种不同的数学模型。 相似文献
11.
《中国海洋大学学报(英文版)》2021,(5)
Oxidative stress is involved in the progression of neurodegenerative diseases. Previous evidences showed that plasmalogens could improve neurodegenerative diseases. In this study, we investigated the function of phosphoethanolamine plasmalogens enriched with EPA(EPA-p PE) and phosphatidylethanolamine enriched with EPA(EPA-PE) on oxidative damage prevention after hydrogen peroxide(H_2 O_2) and tert-butylhydroperoxide(t-BHP) challenge in primary hippocampal neurons. Results showed that neurons pretreated with EPA-p PE and EPA-PE demonstrated the ability to alleviate oxidative damage, which was proved by the increased cell viability. Moreover, the shape and number of neurons were more similar to those of the control group. Antioxidant activity, apoptosis, as well as Trk B/ERK/CREB signaling pathway were investigated to explore the mechanisms. The results suggested that EPA-PE was superior to EPA-p PE in regulating mitochondrial apoptosis. EPA-p PE was more prominent than EPA-PE in upregulating Trk B/ERK/CREB signaling pathway. Phospholipids with EPA exerted neuroprotective effects via inhibiting oxidative stress, suppressing apoptosis, and regulating Trk B/ERK/CREB signaling pathway. Therefore, the results provide a scientific basis for utilization of phospholipids enriched with EPA on the treatment of neurodegenerative disease. 相似文献
12.
测绘营销的各种表现不尽如人意 ,测绘单位面向市场要求得生存与发展 ,地理信息产业要获得进一步发展和升级 ,必然要求加强测绘营销。须采取的措施包括 :优化测绘营销环境 ,调节测绘生产经营机制、增强测绘学科与营销学科的渗透、加强教育界、学术界与测绘管理部门、生产单位在营销方面的交流与合作。 相似文献
13.
同步N-策略多重休假的M/M/C/WV排队 总被引：1，自引：0，他引：1
考虑同步N-策略多重休假和工作休假M/M/c排队,简记为M/M/c（N-WV）。在休假期间,服务员并未完全停止工作而是以较低的速率为顾客服务。用拟生灭过程与矩阵几何解方法,给出了系统稳态队长和稳态条件等待时间的分布。此外,也得到了队长和等待时间的条件随机分解结构及附加队长和附加延迟的分布。 相似文献
14.
The effects of environmental Na /K ratio on the gill ion-transport enzyme activity,plasma osmolality and growth ofCynoglossus semi/aevis juveniles were investigated.The results showed that,plasma osmolality was similar among flounder adaptedto different Na /K ratios of saline groundwaters (P>0.05),while the growth,gill Na ,K -ATPase and HCO,3'--ATPase activities wereaffected by Na /K ratio significantly (P<0.05).The gill Na ,K -ATPase activity reached its maximum on day 3,then decreasedgradually from day 3 to day 9 and remained constant from day 9 to day 15.The peak values of gill Na ,K -ATPase activity weredetected on day 3 for all Na /K ratios of saline groundwaters,then the enzyme activities descended,and on day 9 the enzyme activi-ties achieved steady state,and the gill HCO,3--ATPase activity increased rapidly and achieved steady state after one day.Duringsteady state,the gill Na ,K -ATPase and HCO,3--ATPase activity of Na /K ratios 20 and 40 treatments were significantly higherthan those in the control group (Na /K ratio 27.5),while there were no significant differences between the Na /K ratio 30 treatmentand the control group; the gill Na ,K -ATPase activity of Na /K ratio 20 and 40 treatments were significantly higher than that forratio 30 treatment,but there were no significant differences of gill HCO3-ATPase activity among these treatments.At the end of the15-day experiment,the weight gain (%) and specific growth rate (SGR) of flounders maintained in seawater were significantly higherthan those in groundwaters; significant differences also occurred among the treatments; Na /K ratio 30 treatment had the highestvalues (33.7% and 1.94 respectively),which were significantly higher than those under Na /K ratios 20 and 40 treatments.There-fore,for the saline groundwater used in this experiment,it is suggested that the Na /K ratio be adjusted to approximately 30,I.e.,asclose to that of natural seawater as possible in the culture of flounder. 相似文献
15.
COMPARISON OF HTC METHOD AND UV/PERSULPHATE METHOD TO DETERMINE COLLOIDAL ORGANIC CARBON IN SEAWATER 总被引：1，自引：0，他引：1
ＩＮＴＲＯＤＵＣＴＩＯＮＣｏｌｌｏｉｄｓａｒｅａｂｕｎｄａｎｔｉｎｓｅａｗａｔｅｒ(Ｋｏｉｋｅ,1 990 ;ＷｅｌｌｓａｎｄＧｏｌｄｂｅｒｇ,1 991 ;1 992 ) ,ａｎｄａｌａｒｇｅｆｒａｃ ｔｉｏｎｏｆｄｉｓｓｏｌｖｅｄｏｒｇａｎｉｃｃａｒｂｏｎｉｎｓｅａｗａｔｅｒｉｓｃｏｌｌｏｉｄ.Ｔｈｉｓｆｒａｃｔｉｏｎｏｆｄｉｓｓｏｌｖｅｄｏｒｇａｎｉｃｃａｒｂｏｎｐｌａｙｓａｎｉｍ ｐｏｒｔａｎｔｒｏｌｅｉｎｔｈｅｂｉｏｇｅｏｃｈｅｍｉｃａｌｃｙｃｌｉｎｇｏｆｏｒｇａｎｉｃｃａｒｂｏｎａｎｄｔｒａｃｅｍｅｔａｌｓｉｎ… 相似文献
16.
Effects of fucoidan fromLaminaria japonica on 2,4-dinitrochlorobenzene induced delayed-type hypersensitivity (DTH) reaction and the serum levels of IgG, IgM, complement
C3 and C4 were investigated in the present study. Results showed that oral administration of fucoidan at dose of 150 and 300
mg/(kg· d) for 9 days before the hapten challenge significantly inhibited 2,4-dinitrochlorobenzene induced delayed-type hypersensitivity
reaction; and also inhibited the humoral immunity. Serum C3 and C4 levels were markedly reduced by fucoidan at dose of 150
and 300 mg/kg; and serum IgG and IgM levels were reduced by fucoidan at dose of 300mg/kg. The inhibitory effects of fucoidan
on delayed-type hypersensitivity suggested that it may be potential medication for chronic inflammatory diseases in the future.
This study was supported by National Foundation for New Drug Research & Development (NFND) No.144. 相似文献
17.
首先采用国际上通用的德国地学中心（GFZ）与武汉大学（WHU）精密产品,对GNSS精密卫星轨道和精密钟差产品精度进行初步评估;然后基于WHU精密轨道和钟差产品对18个分布于东半球的MGEX地面站进行多系统定位测试,同时也对BDS的B1I/B3I与B1C/B2a两组新、旧频点的精密单点定位性能进行对比分析。结果表明：1）四大导航系统（GPS、GLONASS、BDS、Galileo）的卫星轨道产品精度均在cm级,精密钟差内符合精度均优于0.1 ns,北斗三号（BDS-3）卫星钟精度相比北斗二号（BDS-2）有显著提升。2）亚太地区BDS的定位精度优于其他3个系统;在其他地区,GPS定位精度最优（与Galileo基本相当）,优于BDS和GLONASS的定位结果。3）BDS PPP平均收敛时间静态模式约为50.33 min、动态模式约为77.83 min,收敛速度略低于GPS、Galileo,优于GLONASS。4）B1C/B2a与B1I/B3I双频消电离层组合PPP定位性能基本相当。 相似文献
18.
Study of horizontal and vertical distributions of the N/P(nitrogen versus phosphate)atom ratio in the northem South Yellow Sea showed that the ratio varied greatly in upper waters of the in-vestigated area and was always much greater than the theoretical Redfield ratio of 16:1 found below the thermocline zone.It was in general higher near the coast and lower in the central part.With increasing depth,the ratio became smaller and smaller.This distribution pattem is attributed to :1)the anthropo-genic influence of the surface N and P which makes the N/P ratio differ from the normal value;2)the easy adsorption of P on particles hinders P trasport to the central part;3)below the thermocline zone,the N and P mainly come from the remineralization of the sedimented phytoplankton residues which have almost the theoretical Redfield value and;4)the existence of the Yellow Sea Bottom Cold Water which inhibits the vertical exchange of the water. 相似文献
19.
We extracted marine low-temperature lysozyme (MLTL), a novel lysozyme, from a marine microorganism through fermentation. Our
previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis. In this
study, we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro. Using zebrafish embryos as an in vivo study model, we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent
manner and that 400 μg/ml MLTL was sufficient to block the growth of SIVs. An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation, migration
and tube formation of HUVECs in a dose-dependent manner. Interestingly, assays by flow cytometry and DNA electrophoresis indicated
that MLTL was able to induce apoptosis of HUVECs. Moreover, further study demonstrated that the disruption of intracellular
Ca2+ homeostasis may play an important role in MLTL induced apoptosis of HUVECs. Taken together, the results of this study demonstrate
for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces
apoptosis through regulation of cellular Ca2+ levels. The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested
that MLTL may be a promising new antiangiogenic agent for use in cancer therapy. 相似文献
20.
利用四川CORS网中ZHIJ、QLAI两个测站数据探讨常用的等权模型、高度角模型以及Helmert验后方差估计模型对BDS/GPS长基线解算精度的影响。结果表明：1）BDS/GPS组合系统可以实现长基线高精度解算；2）Helmert验后方差估计随机模型解算精度最高， BDS/GPS组合系统140 km长基线解算精度在水平向和垂向分别优于8 mm和10 mm；3）等权随机模型、高度角随机模型对BDS/GPS长基线解算性能基本相当。 相似文献