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1.
This sixty-day study was performed to determine the effects of short-term starvation and refeeding cycles on growth, feeding performances and body composition of rainbow trout(O ncorhynchus mykiss). Three hundred trout fingerlings with an average initial weight of 17.5±0.06 g were randomly distributed in 15 circular fiberglass tanks. The fish were exposed to 5 different feeding regimes; control: continuously fed twice daily to apparent satiation; T1 : starved for 1 day and re-fed for 2 days; T2 : starved for 1 day and re-fed for 4 days; T3 : starved for 3 days and re-fed for 12 days; T 4 : starved for 4 days and re-fed for 16 days. At the end of the experiment, growth performance, feed utilization, whole body ash and moisture contents were not significantly(P 0.05) different among the treatments. However, whole body protein content in T3 was significantly higher than other treatments(P 0.05). A significant difference in whole body fat content was observed between T3 and the control group at the end of the experiment(P 0.05). In conclusion this experiment suggests that feeding schedules involving starvation(1–4 days) and re-feeding cycles are a promising feed management tool for rainbow trout culture.  相似文献   

2.
The present trial was conducted with starry fl ounder Platichthys stellatus broodstock to evaluate the effects of dietary vitamin A,ascorbic acid,and α-tocopherol on the gonadal development and reproductive performance.8 000 IU/kg diet vitamin A(VA group),500 mg/kg diet ascorbic acid(Vcpp group),or 250 mg/kg diet α-tocopherol(α-TA group)was added into basal diet to create 3 vitamin experimental diets,respectively.Each diet was fed to 450 starry fl ounder broodstock for 104 days.Samples were collected weekly.The gonadosomatic index(GSI)of 3 vitamin experimental groups fi rst decreased and then increased.Maximum GSI of Vcpp group was higher than that of α-TA group but lower than that of VA group.The spawning periods of 3 vitamin experimental groups lasted 49,56,and 45 days,respectively.No mature eggs were observed in the control group during the trial.The absolute fecundity(AF)and relative fecundity(RF)of α-TA group was higher than that of Vcpp group but lower than that of VA group.The results suggest that different vitamins play different roles in the fi sh reproductive process.Vitamin A stimulated the maturation of the ovary,ascorbic acid prolonged the spawning period,and α-tocopherol affected the development of the eggs.  相似文献   

3.
Bay scallops were introduced from the east coast of the U.S. into China to shorten the economic turn-over from 2 to 1 year. Parent scallops were carried to Qingdao on Dec. 20. 1982 and stocked in indoor tanks at controlled temperature and fed with a mixture of Phaeodictylum tricomutum, Pyrarnmonas sp. and Chhrella sp. They spawned on Jan. 26 of the next year. The larvae were reared at a temperature of 18 -21℃ and fed with Isochrysis galbana, Pyramimonas sp. and Chorella sp. In 4 weeks' growing, the spats averaged 827um. They attained a height of 6.9 mm on May 9. In the middle of May, the seed scallops were transferred to Luoyuan Bay in Fujian Province, and Jiaozhou Bay, as well as the area off Taiping Jiao Cape, Qingdao, Shandong Province for experimental culture in plastic netcages suspended on a single line raft.Bay scallops cultured in . Luoyuan Bay grew to an averaged shell height of 10.4 mm. In Jiaozhou Bay and the Taiping Jiao Cape culture area, they grew to SO mm in average shell height (marketa  相似文献   

4.
LAS degradation rate in Donghu Lake water under aerobic was much faster than under anaerobic condition . The half life of LAS in aerobic and anaerobic environment was 3.2 days and 57 days, respectively . The degradation rate at 25-27℃was approximately 20 times higher than that at 1.5-3.5℃.In a laboratory model aquatic ecosystem, two stages of bioconcentration in fish , daphnia and snail were observed . The first stage , on second day exposure , resulted from accumulation of intact LAS , and the second stage , on 16th day exposure , was due to metabolites . The bioconcentration factor (BCF) of LAS was extremely low in the muscle of hybrid carp (0.64), but rather high (2485) in the gall bladder .  相似文献   

5.
The effects of natural fish oil,DHA oil and soybean lecithin in microparticulate diets on stress tolerance of larval gilthead seabream(Sparus aurata)were investigated after 15 days feeding trials.The tolerance of larval gilthead seabream to various stress factors such as exposure to air(lack of dissolved oxygen),changes in water temperature(low)and salinity(high) were determined.This study showed that microparticulate diet with natural fish oil and soybean lecithin was the most effective for in-creasing the tolerance of larval gilthead seabream to various stresses,and that microparticulate diet with natural fish oil and palmitic acid(16:0)was more effective than microparticulate diet with DHA oil and soybean lecithin.  相似文献   

6.
The early effects of chromosomal manipulation of eggs and sperm on the yields of trip-loid and gynogenefic diploid larvae of Paralichthys olivaceus were investigated. Triploidy was achieved by cold shocking fen.ilized eggs at 0 - 2℃ for 45 minutes duration 5 minutes after fen.ilization, and the in-duced triploidy rates were 31.2% - 50% and the relative hatching rates were 53.3% - 99%. Gynnge-aetic diploids were obtained when eggs were inseminated with irradiated sperm and cold shocked at 0 -2℃ for 45 minutes duration 5 minutes after fertihzation. The induced gynogenetic diploid rates and the relative hatching rates were 94 % - 96 % and 48.5 % - 68.5 % respectively. The embryonic development of the triploid experimental group and of the gynogenetic diploid experimental group was delayed at first compared with the control group. But from the gastrula stage, it was not delayed anymore. There were no significant differences in the growth of the triploid experimental group larvae and the control group larvae, and in the growth of the gynogenetic diploid experimental group larvae and the control group larvae ac-cording to Student‘s t-test (α = 0.05). The relationship between the early growth of the triploid experi-mental group larvae and that of gynogenetic diploid experimental group larvae was also studied.  相似文献   

7.
The cooling and humidifying effects of urban parks are an essential component of city ecosystems in terms of regulating microclimates or mitigating urban heat islands(UHIs).Air temperature and relative humidity are two main factors of thermal environmental comfort and have a critical impact on the urban environmental quality of human settlements.We measured the 2-m height air temperature and relative humidity at the Beijing Olympic Park and a nearby building roof for more than 1 year to elucidate seasonal variations in air temperature and relative humidity,as well as to investigate the outdoor thermal comfort.The results showed that the lawn of the park could,on average,reduce the air temperature by(0.80±0.19)℃,and increase the relative humidity by(5.24±2.91)% relative to the values measured at the building roof during daytime.During the nighttime,the lawn of the park reduced the air temperature by(2.64±0.64)℃ and increased the relative humidity by(10.77±5.20)%.The park was cooler and more humid than surrounding building area,especially in night period(more pronounced cooling with 1.84℃).Additionally,the lawn of the park could improve outdoor thermal comfort through its cooling and humidifying effects.The level of thermal comfort in the park was higher than that around the building roof for a total of 11 days annually in which it was above one or more thermal comfort levels(average reduced human comfort index of 0.92)except during the winter.  相似文献   

8.
The effects of different microbes on fermenting feed for sea cucumber(Apostichopus japonicus) were compared to select the optimal fermentation strain in this study. Saccharomgces cerevisae, Candida utilis, Bacillus subtilis and Geotrichum candidum were independently added into the experimental compound feed, while only saline was mixed with the control feed. The fermentation treatments were inoculated with 10% seed solution under the condition of 25℃ and 70% water content, which lasted for 5 days to elucidate the optimal microbe strain for fermenting effect. Physicochemical indexes and sensorial characteristics were measured per day during the fermentation. The indexes included dry matter recovery(DMR), crude protein(CP), the percentage of amino acid nitrogen to total nitrogen(AA-N/t N), the percentage of ammonia nitrogen to total nitrogen(NH3-N/t N), and the ratio of fermentation strains and vibrios to the total microbes, color, smell and viscosity. The results showed that DMR, CP and AA-N/t N of the S. cerevisae group reached the highest level on day 3, but the ratio of fermentation strain was second to C. utilis group. In addition, its NH3-N/t N and the ratio of vibrios were maintained at low levels, and the sensory evaluation score including smell, color and viscosity was the highest in S. cerevisae group on day 3. Therefore, S. cerevisae could be the optimal strain for the feed fermentation for sea cucumber. This research developed a new production method of fermentation feed for sea cucumber.  相似文献   

9.
Effects of irradiated and non-irradiated Ergosan extract(alginic acid) on rainbow trout growth performance and skin mucosal immunity were compared. Ergosan was irradiated at 30 kGy in a cobalt-60 irradiator. A total of 252 fish(128.03±9.4 g) were randomly divided into four equal groups,given the basal diet either unsupplemented with Ergosan(control group) or supplemented with crude Ergosan(5 g/kg),ethanol-extracted Ergosan(0.33 g/kg) or irradiated Ergosan(0.33 g/kg) according to this protocol: basal diet for 15 days,treatment diet for 15 days,basal diet for 10 days and treatment diet for 15 days. Highest growth performance was observed in fish fed irradiated Ergosan(P0.05). Dietary administration of different Ergosan types did not cause any changes in mucus protein level,but improved alkaline phosphatase level and hemagglutination titer compared with the control(basal diet without Ergosan) on day 55 offeeding trial(P0.05). Furthermore,the highest value of lysozyme activity was observed in gamma-irradiated Ergosan on day 55. In conclusion,gamma-irradiated Ergosan at 0.33 g/kg was found to improve growth performance and mucus biological components significantly in comparison with the control group(basal diet without Ergosan).  相似文献   

10.
The effects of an exogenous probiotic( Bacillus amyloliquefaciens) on microbial community structure of B ranchionus plicatils and A rtemia sinica were evaluated in this study during turbot( Scophthalmus maximus) larval breeding. The analysis and comparison of the microfloral composition of live feed with probiotic was conducted using the Illumina HiSeq PE250. The abundance of microbial species and diversity of microflora in live feed with B. amyloliquefaciens were higher than those in the control. The microfloral composition was similar among the three replicate experimental groups of B. plicatils compared with the control after enrichment. L actococcus, Pseudoalteromonas, and Alteromonas were always dominant. Additionally, some other bacterial species became dominant during the enrichment process. The microbial community during nutrient enrichment of A. sinica was rather similar among the three control replicates. Relative abundance of Cobetia sp., the most dominant species, was 54%–65.2%. Similarity in the microbial community was still high after adding B. amyloliquefaciens. Furthermore, P seudoalteromonas and Alteromonas replaced Cobetia as the dominant species, and the abundance of Cobetia decreased to 4.3%–25.3%. Mean common ratios at the operational taxonomic unit level were 50%–60% between the two B. plicatils and A. sinica treatments. Therefore, the microbial community structure changed after adding B. amyloliquefaciens during nutrient enrichment of B. plicatils or A. sinica and tended to stabilize. Additionally, the abundance of V ibrio in any kind of live feed was not significantly dif ferent from that in the control. These results will help improve the microflora of B. plicatils and A. sinica and can be used to understand the multiple-level transfer role of probiotic species among probiotic products, microflora of live feed, and fish larvae.  相似文献   

11.
Effects of reduced salinity on the oxygen consumption rate (OCR) and the ammonia-N excretion rate (AER) of scallopChlamys farreri (3.2–5.9 cm in shell height, 0.147–1.635 g in soft tissue dry weight) were studied in laboratory from March 21, 1997 to April 16, 1997. Under the controlled conditions of reduced salinity from 31.5 to 15.0 and ambient temperature 17°C and 23°C, the concentrations of dissolved oxygen and ammonia-N were determined by the Winkle method and the hypobromite method, respectively. Results showed that with controlled reduced salinity, the mean values of the OCR were 2.17 mg/(g.h) at 17°C, and 2.86 mg/(g.h) at 23°C and that the mean values of the AER were 178.0 μg/(g.h) at 17°C and 147.0 μg/(g.h) at 23°C. The OCR and the AER decreased with reducing salinity from 31.5 to 15.0 both at 17°C and 23°C. The effects of reduced salinity on the OCR and the AER of scallopC. farreri could be represented by the allometric equation and the exponential equation, respectively. Contribution No. 3295 from the Institute of Oceanology, Chinese Academy of Sciences. Project supported by the National Ministry of Science and Technology of China and by the Chinese Academy of Sciences, Grant No. 96-922-02-04 and KZ951-A1-102-02.  相似文献   

12.
Effects of temperature on oxygen consumption rate (OCR) and ammonia—N excretion rate of scallopChlamys farreri (1.7–6.2 cm in shell height) were studied in laboratory from Dec. 30,1996 to Jan. 28,1997. Under the controlled conditions of ambient water temperature 10–31°C and salinity 32, the concentrations of dissolved oxygen and ammonia—N were determined by the Winkle method and the hypobromite method, respectively. Results showed that the OCR ranged from 1.20 mg/g (DW) · h to 5.76 mg/g (DW) · h. The OCR increased with temperature from 10°C to 23°C, but at 28°C the OCR of mature individuals decreased, and that of different size scallops reduced at 31°C. The ammonia—N excretion rate ranged from 113.03 μg NH4-N/g (DW) · h to 486.63 μg NH4-N/g (DW) · h, and increased with temperature from 10°C to 31°C. Contribution No.3180 from the Institute of Oceanology, Chinese Academy of Sciences. This work was supported by the National Commission of Science and Technology of China, Grant No.96-922-02-04.  相似文献   

13.
Histological characteristics of gametogenesis of two kinds of scallops, gonochoric Chinese scallop, Chlamys farreri and hermaphroditic bay scallop, Argopecten irradians were investigated in this study. Spermatogenesis in C. farreri has different developmental stages: spermatogonia, primary spermatocyte, second spermatocyte, spermatid and spermatozoon. A large number of same developmental stage spermatic cells converge at a definite area of the testis. Premeiotic, previtellogenic and vitellogenic oocytes can be found during oogenesis in C. farreri, where oocyte distribution is obviously irregular. The A. irradians gonad consists of two different parts in one individual: one part functions as testis, the other as ovary. Between these two parts is a special appearance area, where a large number of spermatic cells are bound with two layers of acellular substance with many oocytes in it.  相似文献   

14.
Tetraploid induction was carried out by inhibiting mitosis I in fertilized eggs ofChlamys farreri. Mitosis I was blocked with cold shock (5–7°C), Cytochalasin B (0.75 mg/L) and 6-dimethylaminopurine (6-DMAP) (60–75 mg/L) when 60% fertilized eggs released polar body II at 20°C. At 4-cells embryo stage, the ploidy was determined by counting chromosome number. In control groups, most embryos were diploids (72.22%) and aneuploids (24.78%). In Cytochalasin B, cold shock and 6-DMAP treated groups, tetraploids were respectively 10.51%, 4.08%, and 13.34%; aneuploids were 43.10%, 35.93% and 29.16%, and triploids were 7.84%, 8.52% and 18.33%. At D-larva stage, ploidy was determined by flow cytometry (FCM). The ploidy analysis of day 2 larvae showed diploids in control group and also in three treated groups. Juvenile scallops (0.2–0.3cm) which were harvested in two control groups and two CB treated groups were all diploids through checking ploidy individually by FCM. Contribution No. 3648 from the Institute of Oceanology, Chinese Academy of Sciences. Contribution No. 238 from the Experimental Marine Biology Laboratory, Chinese Academy of Sciences. Project funded by grant (No. 819-01-07) from the Chinese Science and Technology Ministry and also by “Hundred Talents Plan” grant from the Chinese Academy of Sciences.  相似文献   

15.
Triploidy was induced in Chinese shrimpFenneropenaeus chinensis by 30±0.5°C heat shock treatment (initiated at 20 min after fertilization) for 10 min to inhibit the release of PB2 at 18.0°C. The highest triploid rate obtained was 84.5% in nauplius stage. The effect of heat shock treatment on meiosis and cleavage of eggs was investigated in this work aimed to establish efficient procedures for triploid induction and to gain understanding of the mechanism of triploid production. Three pronuclei that could be observed in the treated eggs under fluorescence microscope developed into triploid embryos. Some abnormal chromosome behavior was observed in heat shocked eggs. Contribution No. 4407 from the Institute of Oceanology, Chinese Academy of Sciences. This work was supported by Project under Major State Basic Development Program (G1999012009) and Knowledge Innovation Program of the Chinese Academy of Sciences (KZCX 2-211)  相似文献   

16.
Heat shocks effectively produced triploids inPenaeus chinensis. Fertilized eggs heat shocked (28–32°C) for 8 to 16 minutes, starting from 8 to 20 minutes after spawning, resulted in triploidy induction rates of 39%–75%. Several triploid populations were cultured to 10 cm. In a triploid population, two kinds of ovaries were observed. Histological examination showed apparent differences between these two kinds of ovaries, whereas among male shrimp, there were no such differences. Contribution No. 3465 from the Institute of Oceanology. Chinese Academy of Sciences. The study was supported by the International Foundation for Science, Asian Fisheries Society and Chinese Climbing Project PDB 6–2, and Chinese National 863 Project (No. 863-819-01-05).  相似文献   

17.
Survival, growth and immune response of the scallop, Chlamys farreri, cultured in lantern nets at five different depths (2, 5, 10, 15, and 20 m below the sea surface) were studied in Haizhou Bay during the hot season (summer and autumn) of 2007. Survival and growth rates were quantified bimonthly. Immune activities in hemolymph (superoxide dismutase (SOD) and acid phosphatase (ACP)) were measured to evaluate the health of scallops at the end of the study. Environmental parameters at the five depths were also monitored during the experiment. Mortalities mainly occurred during summer. Survival of scallops suspended at 15 m (78.0%) and 20 m (86.7%) was significantly higher than at 2 m (62.9%), 5 m (60.8%) or 10 m (66.8%) at the end of the study. Mean shell height grew significantly faster at 10 m (205.0 μm/d) and 20 m (236.9 μm/d) than at 2, 5 or 15 m in summer (July 9 to September 1); however, shell growth rate at 20 m was significantly lower than at the other four depths in autumn (September 2 to November 6). In contrast to summer, scallops at 5 m grew faster (262.9 μm/d) during autumn. The growth of soft tissue at different depths showed a similar trend to the shell. Growth rates of shell height and soft tissue were faster in autumn than in summer, with the exception of shell height at 20 m. SOD activity of scallops increased with depth, and ACP activity was significantly higher at 15 and 20 m than at other depths, which suggests that scallops were healthier near the bottom. Factors explaining the depth-related mortality and growth of scallops are also discussed. We conclude that the mass mortality of scallop, C. farreri, during summer can be prevented by moving the culture area to deeper water and yield can be maximized by suspending the scallops in deep water during summer and then transferring them to shallow water in autumn.  相似文献   

18.
Results of neutron activation analysis (NAA) ofSargassum thumbergii samples collected from the Qingdao Taipingjiao coast on March 20, 1996 showed that (1)Sargassum thunbergii can to some extent accumulate potassium and calcium (the accumulation coefficient was 10); (2) it can accumulate almost all trace elements, especially iron, managanese and zinc; (3) it can accumulate strontium, aluminium thorium, and rare earth elements. Contribution No.3308 from the Institute of Oceanology, Chinese Academy of Sciences. The study supported by the National Ninth-Five-Year Key Project “96-916-04-01”.  相似文献   

19.
Growth of black porgy,Sparus macrocephalus, was studied experimentally at four ration levels from starvation to satiation and four temperatures ranging from 14.8 to 26.8°C. At maximum rations, the specific growth rate (SGR) increased with temperature and was a negative linear function of body weight. The relationship between SGR and ration levels was a decelerating curve. Stepwise regression was used to develop the predictive models for the specific growth rates. Maintenance rations and optimum rations both increased with increased temperature. The relationship between maintenance, optimum rations and body weight was influenced by temperature. Conversion efficiencies increased with ration from zero at maintenance ration to a peak at the optimum ration, then declined with further increased ration. Contribution No. 3127 from the Institute of Oceanology, Chinese Academy of Sciences. Project 39370542 supported by NSFC and study also supported by National Key Laboratory in the Institute of Hydrobiology, Chinese Academy of Sciences.  相似文献   

20.
Scrippsiella trochoidea (Stein) Loeblich III was grown in a nitrogen-or phosphorus-limited batch culture system in laboratory. Growth rates and cellular Chl-a were measured as functions of nitrate and phosphate concentrations. Growth rates were hyperbolic with both nitrate and phosphate concentration and fit the Monod equation. The minimum cell quota of nitrogen and phosphorus and then the optimum N∶P ratio ofS. trochoidea were estimated in this study. Measurement of phosphate concentration in Jiaozhou Bay suggest that phosphorus is the limiting factor ofS. trochoidea growth. Contribution No. 3679 from the Institute of Oceanology, Chinese Academy of Sciences. Project 39790110 supported by NSFC, the study aslo supported by the National Climbing Project B (PDBG-7-2) and partly supported by an MF grant from Hong Kong University of Science and Technology.  相似文献   

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