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参对光照变化非常敏感，研究刺参对光照的分子响应非常重要。本研究应用RNA测序获取了刺参暴露于强光（“强光”）、正常光照（“对照”）和完全黑暗（“黑暗”）环境下体壁的基因表达谱情况，通过“对照”与“黑暗”，“对照”与“强光”和“黑暗”与“强光”的比较，在|log2 ratio|≥1和FDR≤0.001的标准下分别发现了1161、113和1705个差异表达基因（DEGs）。基因本体分析表明，“cellular process”和“binding”在“生物过程”和“分子功能”类别中的DEGs富集最多，而“cell”和“cell part”在“细胞组分”类别中的DEGs富集最多。将DEGs与Kyoto Encyclopedia基因和基因组数据库上的于214、41和229条通路进行比对，发现了51、2和57条通路分别显著富集。本研究发现的光特异性DEGs可作为深入研究刺参对光照变化的生化适应机制的重要目标基因。 相似文献
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Apostichopus japonicus(Holothuroidea,Echinodermata) is an ecological and economic species in East Asia.Conventional biometric monitoring method includes diving for samples and weighing above water,with highly variable in weight measurement due to variation in the quantity of water in the respiratory tree and intestinal content of this species.Recently,video survey method has been applied widely in biometric detection on underwater benthos.However,because of the high flexibility of A.japonicus body,video survey method of monitoring is less used in sea cucumber.In this study,we designed a model to evaluate the wet weight of A.japonicus,using machine vision technology combined with a support vector machine(SVM) that can be used infield surveys on the A.japonicus population.Continuous dorsal images of free-moving A.japonicus individuals in seawater were captured,which also allows for the development of images of the core body edge as well as thorn segmentation.Parameters that include body length,body breadth,perimeter and area,were extracted from the core body edge images and used in SVM regression,to predict the weight of A.japonicus and for comparison with a power model.Results indicate that the use of SVM for predicting the weight of 33 A.japonicus individuals is accurate(R~2=0.99) and compatible with the power model(R~2=0.96).The image-based analysis and size-weight regression models in this study may be useful in body weight evaluation of A.japonicus in lab and field study. 相似文献
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在实验室条件下,研究了全光照(24L︰0D)、半光照半黑暗(12L︰12D)、全黑暗(0L︰24D)3种光照周期下刺参(Apostichopus japonicus)的摄食节律、摄食率以及3种消化酶(脂肪酶、淀粉酶、胰蛋白酶)活力的昼夜变化规律。结果显示:(1)在全光照条件下,刺参极少活动,24 h内的摄食比例较低(6%~10%);在半光照半黑暗条件下,刺参光照阶段运动不活跃,摄食比例较低(0~10%),黑暗阶段运动极其活跃,摄食比例较高;在全黑暗条件下,刺参一直处于较活跃状态,24 h内的摄食比例都较高。3种光照条件下,刺参的摄食高峰都出现在00:00~02:00,且刺参夜晚的摄食比例显著高于白天(P0.05);(2)刺参的日摄食率从全光照、半光照半黑暗到全黑暗依次增加,3种光照周期条件下刺参夜晚的摄食率极显著高于白天的摄食率(P0.01);(3)3种光照周期下,刺参3种消化酶活力的最大值都出现在22:00~02:00,消化酶活力的最大值比摄食高峰早0~4 h。研究结果表明,光照对刺参的摄食节律和消化酶活力昼夜变化都有显著影响,刺参具有提前分泌消化酶为即将到来的摄食做好准备的调节机制。 相似文献
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Survival, growth and immune response of the scallop, Chlamys farreri, cultured in lantern nets at five different depths (2, 5, 10, 15, and 20 m below the sea surface) were studied in Haizhou Bay during the hot season (summer and autumn) of 2007. Survival and growth rates were quantified bimonthly. Immune activities in hemolymph (superoxide dismutase (SOD) and acid phosphatase (ACP)) were measured to evaluate the health of scallops at the end of the study. Environmental parameters at the five depths were also monitored during the experiment. Mortalities mainly occurred during summer. Survival of scallops suspended at 15 m (78.0%) and 20 m (86.7%) was significantly higher than at 2 m (62.9%), 5 m (60.8%) or 10 m (66.8%) at the end of the study. Mean shell height grew significantly faster at 10 m (205.0 μm/d) and 20 m (236.9 μm/d) than at 2, 5 or 15 m in summer (July 9 to September 1); however, shell growth rate at 20 m was significantly lower than at the other four depths in autumn (September 2 to November 6). In contrast to summer, scallops at 5 m grew faster (262.9 μm/d) during autumn. The growth of soft tissue at different depths showed a similar trend to the shell. Growth rates of shell height and soft tissue were faster in autumn than in summer, with the exception of shell height at 20 m. SOD activity of scallops increased with depth, and ACP activity was significantly higher at 15 and 20 m than at other depths, which suggests that scallops were healthier near the bottom. Factors explaining the depth-related mortality and growth of scallops are also discussed. We conclude that the mass mortality of scallop, C. farreri, during summer can be prevented by moving the culture area to deeper water and yield can be maximized by suspending the scallops in deep water during summer and then transferring them to shallow water in autumn. 相似文献
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随着智能手机的普及,基于手机的室内定位应用越来越受到各界的关注,并在各个领域得到快速的应用。基于手机的室内定位技术在商业广场线上线下融合、安全监控、个人服务等方面都有着极为核心的作用。首先介绍了室内位置服务应用平台的架构体系,其次介绍了手机室内定位的常用技术及其基本原理,最后介绍了室内定位在商场、机场、个人服务等方面的典型应用,并进行了总结。 相似文献
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An artificial oyster shell reef was deployed in Rongcheng Bay, East China. However, the effects of this reef on the surrounding macrobenthic communities were unknown. We compared sedimentary factors, macrobenthic biomass, abundance, and community composition and ecological indicators between the reef and non-reef areas over a one year period. The mean values for chlorophyll a （Chl a）, total organic matter （TOM）, total organic carbon （TOC）, and total nitrogen （TN） content in surface sediments in the reef area were slightly higher than those in the non-reef area. The Chl a levels differed significantly between the two areas, but the TOM, TOC, and TN were not significantly different. The abundance of crustaceans was significantly different between the two areas, but the abundance and biomass ofpolychaetes, echinoderms, mollusk did not differ significantly. The permutational multivariate analysis of variance （PERMANOVA） revealed that the macrobenthic community differed significantly through time and analysis of similarity multivariate analyses （ANOSIM） revealed that the macrobenthic community differed significantly in some months. The ecological indicators revealed that the environmental quality of the reef area was slightly better than that of the non-reef area. Overall, our results suggest that the artificial oyster shell reef may change the macrobenthic community and the quality of the environment. Despite the lack of an effect in the short term, long-term monitoring is still needed to evaluate the effects of artificial oyster shell reefs on macrobenthic communities. 相似文献
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为了研究人工鱼礁区基本水动力特征,本文利用人工鱼礁投放区的座底海床基获取半年以上的水动力观测资料,通过谱分析、调和分析、滤波等方法分析了该海区潮汐、潮流特征,并讨论了余流特征。结果表明,该海域属于不规则半日潮,具有显著的全日潮周期和半日潮周期,潮汐性质指数为0.98,平均潮差为0.95m,最大可能潮差为2.25m。潮流为典型的往复潮,潮流主向为NNE-SSW,优势分潮为M2分潮。垂向上,流速大小随深度增加显著降低,海底1m的流速较表层降低约30%,流向向沿鱼礁布设方向偏转。该海域余流较小,欧拉余流与斯托克斯余流大小相当,分别为1.35cm/s,1.41cm/s,均为向岸输运,欧拉余流表层受风影响较大,拉格朗日余流为2.76cm/s,方向SEE。该海域流场垂向结构与人工鱼礁投放后底摩擦增加、鱼礁对近底层水流的阻滞作用有关。底层温度具有显著的季节变化与日变化特征,短期高频变化存在显著的全日周期与半日周期,冬春季由于垂向混合加强,全日信号更为显著。底层浊度的升高主要由大风过程加强垂向混合的引起。 相似文献
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为探究刺参(Apostichopusjaponicus)种内竞争与栖息地对其群体分布的影响,作者通过缩时摄像机拍摄及视频分析,研究了刺参集群分布特征,结果发现:刺参在礁石栖息地的栖息时间显著高于在缸壁处的栖息时间(P 0.05),刺参对栖息地有明显偏好性;刺参在运动时并未聚集在同一栖息地,而离散分布在多处栖息地中(P 0.05);当密度增大到15头/缸(11.31头/m~2, d=1.3 m)时,刺参在栖息地内的平均聚集率和平均聚集时间比例会显著下降(P 0.05),种内竞争加剧,刺参多呈斑块状分布,在密度过高时种内竞争压力上升,刺参个体间选择分散以减少竞争。本研究查明了实验室条件下栖息地分布和种内竞争对刺参集群特征的影响,可为野外刺参增养殖与资源持续利用提供理论参考。 相似文献