共查询到20条相似文献，搜索用时 46 毫秒
1.
目的：建立测定宁志胶囊中芒果苷含量的HPLC法。方法：采用高效液相色谱法,色谱柱为Agilent 5TC-C18（250 mm×4.6 mm,5 μm）,以乙腈-0.2%冰醋酸溶液（13∶87）为流动相,检测波长为258 nm,流速为1.0 ml/min,柱温为30 ℃。结果：芒果苷在4.0951～51.1885 μg/ml之间呈良好线性关系（r=0.99987）。芒果苷平均加样回收率为104.92%（n=6）,RSD=1.20%。结论：经方法学验证,本法可用于宁志胶囊的质量控制。 相似文献
2.
目的：建立高效液相色谱（HPLC）法测定疏肝健脾解毒方中毛蕊异黄酮葡萄糖苷的含量。方法：采用HPLC法,以依利特Supersil ODS-C18柱（4.6 mm×250 mm,5 μm）为色谱柱,以乙腈-0.2%甲酸溶液为流动相梯度洗脱（0～23 min,12%乙腈;23～65 min,12%～26%乙腈）,流速为1.0 ml/min,柱温为30℃,检测波长为260 nm。结果：经方法学验证,毛蕊异黄酮葡萄糖苷在7.2～43.2 μg（R2=0.9998）范围内线性关系良好,精密度、稳定性和重复性试验的RSD 均＜2.0%（n=6）,加样回收率为98.87%（RSD为1.79%）。结论：本研究建立的含量测定方法简便,结果准确可靠,适用于疏肝健脾解毒方中毛蕊异黄酮葡萄糖苷的测定,可为其质量标准的深入研究提供依据。 相似文献
3.
目的：建立护心康片多波长HPLC指纹图谱,并研究复方与组方药物指纹图谱峰的相关性。方法：采用C18色谱柱（4.6mm×200mm,5μm）;柱温35℃;0.1%磷酸溶液（A）-乙腈（B）为流动相梯度洗脱;流速1.0ml/min;检测波长分别为203nm（检测三七和黄芪）、270nm（检测丹参酮类）、286nm（检测丹参酚酸类和黄芪）。结果：建立了23个特征峰的护心康片HPLC指纹图谱,并对其特征峰进行了组方药物归属分析。结论：该方法能揭示复方主要色谱峰在组方药物中的归属,有效控制护心康片的质量。 相似文献
4.
目的：建立四甲丸中2种活性成分哈巴俄苷、肉桂酸的含量测定方法。方法：色谱条件为C18色谱柱（4.6mm×250mm,5μm）;流动相为乙腈-1%醋酸水溶液,体积比为25∶75,等度洗脱;流速：1mL/min,柱温：25℃;检测波长：278nm。结果：在该色谱条件下,哈巴俄苷、肉桂酸分离良好,质量浓度分别在5.36～134.06μg/mL（r=0.9999）、5.28～131.95μg/mL（r=0.9997）时,与其峰面积线性关系良好。加样回收率分别为103.6%（1.20%）、100.4%（1.30%）（n=6）。结论：本试验所建立的方法简便、准确、重复性好,可用于四甲丸的质量控制。 相似文献
5.
目的：建立炎可宁丸质量标准。方法：采用显微鉴别法鉴别黄连。采用薄层色谱法（TLC）鉴别黄柏、大黄、黄芩、板蓝根、黄连。采用高效液相色谱（HPLC）法测定大黄素与大黄酚的含量,使用Phenomenex Luna C18色谱柱（4.6 mm ×250 mm,5μm）,以甲醇-0.1%磷酸溶液（85∶15）为流动相,流速为1.0 ml/min,柱温为30℃,检测波长为254 nm。采用HPLC法测定黄芩苷的含量,使用CAPCELL PAK C18色谱柱（4.6 mm×250 mm,5μm）,以甲醇-0.1%磷酸溶液(45∶55)为流动相,流速为1.0 ml/min,柱温为30℃,检测波长为280 nm。结果:薄层色谱斑点清晰;大黄素和大黄素酚的线性范围分别为9.3～465.0 ng、11.6～580.0 ng,加样回收率分别为93.3%、98.0%,RSD分别为1.8%、2.7%;黄芩苷的线性范围为68～3400 ng,加样回收率为97.2%,RSD为1.2%。结论:本法简便可靠、结果准确,可用于该制剂的质量控制。 相似文献
6.
目的：提升天麻头风灵胶囊质量标准,有效控制其制剂质量。方法：采用薄层色谱法,以紫丁香苷和哈巴俄苷为对照,定性鉴别槲寄生和玄参;采用HPLC法对阿魏酸进行含量测定,C18柱（Inertsil ODS-2;4.6mm×250mm,5μm）;流动相：乙腈-0.05%磷酸溶液（16∶84）;检测波长：321nm;流速：1ml/min;柱温：30℃。结果：薄层色谱定性鉴别方法灵敏,专属性强;阿魏酸在9.97～997ng范围内线性良好,r=0.9999;加样回收率为97.52%。结论：本方法简便、重复性好,为天麻头风灵胶囊质量标准提升奠定了基础。 相似文献
7.
目的：建立毛茛中白头翁素的含量测定方法。方法：采用Merck Hibar C18色谱柱（4.6mm×250mm,5μm）,流动相为甲醇-水（30∶70）,检测波长220nm,流速0.8ml/min,柱温30℃,进样量20μL。理论板数按白头翁素计≥4000。结果：白头翁素在0.342～8.108mg/ml范围内线性关系良好,平均回收率为98.7%（n=9）,相对标准偏差（RSD）为1.2%。结论：该方法简单、精密度高,结果准确可靠,可作为毛茛中白头翁素含量测定的一种有效方法。 相似文献
8.
目的：优化苦参配方颗粒中苦参碱和氧化苦参碱含量的测定方法。方法：采用HPLC法,色谱柱为Agilent ZORBAX NH2色谱柱(250 mm×4.6 mm,5 μm),流动相：乙腈-无水乙醇-3%磷酸溶液（80∶10∶10）;流速：1 ml/min;检测波长：220 nm;柱温：30℃,进样量：5μl。结果：苦参碱在73.620～736.200 ng范围内线性关系良好,回归方程为y=548.2629x-1200.6932,R=0.9999。氧化苦参碱在79.511～795.110 ng范围内线性关系良好,回归方程为y=533.4244x-560.8712,R=0.9999。精密度、重复性、稳定性试验RSD均<2%;苦参配方颗粒中苦参碱加样回收率在98%～103%之间,RSD为1.52%,氧化苦参碱加样回收率在99%～103%之间,RSD为1.17%。结论：该方法简便快捷,准确度高,重复性好,可为相关测定方法的改进提供参考。 相似文献
9.
目的:建立筋骨痛巴布膏的质量标准。方法：筛选方中独活、大黄、苏木及冰片进行薄层色谱鉴别;HPLC法对成品中大黄素进行含量测定。结果：制定了巴布膏中独活等药材的薄层鉴别方法;以大黄素为指标,以Kromasil C18色谱柱（250 mm×4.6 mm,5 μm）为固定相,甲醇-0.1%磷酸溶液（85∶15）为流动相;检测波长254 nm条件下,成品中大黄素能有效分离,方法学结果可行。结论:该法方便准确、灵敏度高、重现性好,能够为筋骨痛巴布膏质量标准的制订提供客观精准的依据。 相似文献
10.
目的：采用双波长HPLC同时测定参橘草营养强化饮液中5种化合物(腺苷、虫草素、人参皂苷Rg1、人参皂苷Re、橙皮苷)的含量。方法:依利特Hypersil BDS C18色谱柱(4.6 mm×250 mm,5 μm);流动相：乙腈-水,梯度洗脱;流速：1.0 ml/min;柱温25℃;检测波长203 nm(人参皂苷Rg1、人参皂苷Re),260 nm(腺苷、虫草素、橙皮苷)。结果：5种化合物在各自的浓度范围内均呈现良好的线性关系(r>0.9990),平均加样回收率（n=6）分别为96.72%、97.93%、98.04%、99.22%、101.69%。结论：该法简单快捷、稳定可靠,准确率高,重复性好,可用于参橘草营养强化饮液的质量评价。 相似文献
11.
Chandralata Raghukumar G. Sheelu P. A. Loka Bharathi Shanta Nair C. Mohandass 《Marine Georesources & Geotechnology》2001,19(1):1-16
In order to assess the impact of deep-sea mining on the in situ benthic life, we measured the microbial standing stock and concentration of organic nutrients in the deep-sea sediments of the Central Indian Ocean Basin in the Indian pioneer area. Sediments were collected using box core and grab samples during September 1996. The total bacterial numbers ranged from 10 10 -10 11 cells per g -1 dry weight sediment. There was a marginal decrease in the number of bacteria from surface to 30 cm depth, though the subsurface section registered a higher number than did the surface. The highest numbers were encountered at depths of 4-8 cm. The retrievable number of bacteria were two orders less in comparison with the direct total counts of bacteria. An almost homogeneous distribution of bacteria, total organic carbon, living biomass, and lipids throughout the depth of cores indicates active microbial and benthic processes in the deep sea sediments. On the other hand, a uniform distribution of total counts of bacteria, carbohydrates, and total organic carbon in all the cores indicates their stable nature and suggests that they can serve as useful parameters for long-term monitoring of the area after the benthic disturbance. Further studies on temporal variability in this region would not only verify the observed norms of distribution of these variables but would also help to understand restabilization processes after the simulated benthic disturbance. 相似文献
12.
海上大直径钢管桩打桩过程中,桩周土体受到强烈扰动而发生强度弱化,掌握桩周土体强度弱化规律对于准确预测打桩过程、保证工程安全具有重要意义。为研究土体强度弱化规律,开展了环剪试验模拟打桩对桩周土体的扰动,测试土体强度随剪切速率的变化规律,建立了描述土体强度弱化规律的拟合公式,引入到打桩分析软件中。研究结果表明：土体的强度折减程度不仅与土体本身的性质有关还受到土体的埋深和剪切速率的影响,埋深越深土体强度折减程度越低,剪切速率越高土体强度折减越高,在打桩分析中可采用这里推荐的线性折减方法来模拟不同深度处土体强度的折减规律。 相似文献
13.
Geoacoustic Inversion via Genetic Algorithm and Its Application to Manganese Sediment Identification
An acoustic inversion method using a wide-band signal and two near field receivers is proposed and applied to multiple layered seabed models including a manganese sediment. The inversion problem can be formulated into a probabilistic model comprised of signals, a forward model, and additive noise. The forward model simulates wide-band signals, such as chirp signals, and is chosen to be the source-waveletconvolution plane wave modeling method. The wavelet matching technique, using weighted least-squares fitting, estimates the sediment sound-speed and thickness on which determination of the possible numerical ranges for a priori uniform distribution is based. The genetic algorithm is applied to a global optimization problem to find a maximum a posteriori solution for determined a priori search space. Here the object function is defined by an L 2 norm of the difference between measured and modeled signals. Not only the marginal pdf but also its statistics are calculated by numerical evaluation of integrals using the samples selected during importance sampling process of the genetic algorithm. 相似文献
14.
Derek V. Ellis 《Marine Georesources & Geotechnology》2001,19(1):51-63
This article reviews information recently available from existing marine and coastal mining for responses to environmental issues affecting marine mining at different depths. It is particularly but not exclusively concerned with those issues affecting seabed biodiversity impact and recovery. Much information has been gathered in the past 10 years from shallow mining operations for construction aggregate, diamonds, and gold, from coastal mines discharging tailings to shallow and deep water, and from experimental deep mining tests. The responses to issues identified are summarized in a series of eight tables intended to facilitate site-specific consideration. Since impacts can spread widely in the surface mixing layer SML, and can affect the biologically productive euphotic zone, the main issues considered arise from the depth of mining relative to the SML of the sea. Where mining is below the SML, the issue is whether it is environmentally better to bring the extraction products to the surface vessel for processing (and waste discharge), or to process the extraction products as much as possible on the seabed. Responses to the issues need to be sitespecific, and dependent on adequate preoperational environmental impact and recovery prediction. For deep tailings disposal from a surface vessel, there are four important environmental unknowns: (1) the possible growth of "marine snow" (bacterial flocs) utilizing the enormous quantities of fine tailings particles (hundreds or thousands of metric tons per day) as nuclei for growth, (2) the possibility that local keystone plankton and nekton species may migrate diurnally down to and beyond the depth of deep discharge and hence be subjected to tailings impact at depth, (3) the burrow-up capability of deep benthos and their ability to survive high rates of tailings deposition, and (4) the pattern and rate of dispersion of a tailings density current through the deep water column from discharge point to seabed. Actions to obtain relevant information in general and site-specifically are suggested. 相似文献
15.
G. Parthiban 《Marine Georesources & Geotechnology》2000,18(3):223-235
Particle fluxes were measured 7 m above the sea bottom during the predisturbance, disturbance, and postdisturbance periods by using time series sediment traps attached to seven deep-sea moorings deployed in the INDEX experiment site in the Central Indian Basin. The predisturbance particle fluxes varied between 22.3 to 55.1 mg m -2 day -1 . Increased and variable particle fluxes were recorded by the sediment traps during the disturbance period. The increase observed was 0.5 to 4 times more than the background predisturbance fluxes. The increases in particle fluxes (~4 times) recorded by the sediment trap located in the southwestern direction (DMS-1) were the greatest, which could be the result of preferential movement of resuspended particles generated during the deep-sea benthic disturbance along the general current direction prevailing in this area during the experimental period. Also, the traps located closer to the disturbance area recorded greater fluxes than did the traps far away, across the Deep Sea Sediment Resuspension System path. This variability in recorded particle fluxes by the traps around the disturbance area clearly indicates that physical characteristics such as grain size and density of the resuspended particles produced during the disturbance had an important effect on particle movement. The postdisturbance measurements during ~5 days showed a reduction in particle fluxes of ~50%, indicating rapid particle settlement. 相似文献
16.
17.
18.
Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans 总被引：1，自引：0，他引：1
A set of 27 marine planktonic bacteria isolated from the polar regions was characterized by 16S rDNA sequencing and physiological and biochemical testing. More than half of these bacteria were positive for caseinase, gelatinase and 13-glucosidase, and could utilize glucose, maltose or malic acid as carbon source for cell growth. Twelve isolates expressed nitrate reduction activities. Except for one antarctic isolate BSwlO175 belonging to Actinobacteria phylum, these isolates were classified as γ-Proteobacteria, suggesting that γ-Proteobacteria dominated in cultivable marine bacterioplankton at both poles. Genus Pseudoalteromonas was the predominant group in the Chukchi Sea and the Bering Sea, and genus ShewaneUa dominated in cultivable bacterioplankton in the Prydz Bay. With sequence similarities above 97%, genus Psychrobacter was found at both poles. These 27 isolates were psychrotolerant, and significant 16S rDNA sequence similarities were found not only between arctic and antarctic marine bacteria （ 〉 99% ）, but also between polar marine bacteria and bacteria from other aquatic environments （ ≥ 98.8% ）, including temperate ocean, deep sea, pond and lake, suggesting that in the polar oceans less temperature-sensitive bacteria may be cosmopolitan and have a bipolar, even global, distribution at the species level. 相似文献
19.
The advanced piston corer (APC) has been used by the Ocean Drilling Program since 1985 for recovering soft sediments from the ocean floor. The pullout force measured on extracting the core barrel from the sediment is shown to correlate with the average shear strength of the sediment core measured in the ship's laboratory. A simple rule of thumb is derived relating the shear strength of the sediment to the pullout force. Multiple APC holes at individual sites allow the consistency of the pullout measurements to be assessed. The effects of different operational procedures during APC coring are also explored. Although generally applicable, the correlation between pullout force and laboratory measurements of shear strength breaks down for some APC holes, possibly because of the disturbance of some sediment types during the APC coring process. A better understanding of the physical process of APC coring, and its effect on the properties of the sediment both inside and immediately outside the core barrel, would indicate what confidence can be put on the measurement of pullout force as a way of evaluating the in situ shear strength of deep sea sediments. 相似文献
20.
Oedometer tests have been carried out on 70 undisturbed surficial clays (at approximately 250 mm below the mudline), mostly collected by free-fall corers from sites widely scattered throughout the deep-sea North Atlantic. Acoustic measurements were also made, initially on contiguous samples and ultimately on the same sample using a geophysically instrumented oedometer which also collected electrical resistivity data. Apart from those quiescent areas below the carbonate compensation depth, such as north of the West Indies where very fine clays exist, most of the samples are silty clays whose geotechnical-geophysical properties are dependent on the type of clay minerals present (and their ability to take in moisture), the sand-size fraction, and the quantity of carbonate present. Thus the pure clays have high compressibilities which decrease on the addition of coarse particles, while the converse is true for the acoustic parameters, these increasing with the sand fraction. Using the notion of the intrinsic compression line for all samples, and comparison to it of the measured compression curves, it is clear that, contrary to some previously held ideas, most deep-sea clays are normally consolidated; the addition of carbonate has the effect of creating an open, stronger sediment skeleton. Interestingly, where information is available, the variation with depth of a sample's acoustic velocity follows the void ratio pressure relationship of the compression curve. This allows the construction of an in-situ sediment compression curve using the in-situ geophysical observations. 相似文献