共查询到20条相似文献，搜索用时 93 毫秒
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目的：建立炎可宁丸质量标准。方法：采用显微鉴别法鉴别黄连。采用薄层色谱法（TLC）鉴别黄柏、大黄、黄芩、板蓝根、黄连。采用高效液相色谱（HPLC）法测定大黄素与大黄酚的含量,使用Phenomenex Luna C18色谱柱（4.6 mm ×250 mm,5μm）,以甲醇-0.1%磷酸溶液（85∶15）为流动相,流速为1.0 ml/min,柱温为30℃,检测波长为254 nm。采用HPLC法测定黄芩苷的含量,使用CAPCELL PAK C18色谱柱（4.6 mm×250 mm,5μm）,以甲醇-0.1%磷酸溶液(45∶55)为流动相,流速为1.0 ml/min,柱温为30℃,检测波长为280 nm。结果:薄层色谱斑点清晰;大黄素和大黄素酚的线性范围分别为9.3～465.0 ng、11.6～580.0 ng,加样回收率分别为93.3%、98.0%,RSD分别为1.8%、2.7%;黄芩苷的线性范围为68～3400 ng,加样回收率为97.2%,RSD为1.2%。结论:本法简便可靠、结果准确,可用于该制剂的质量控制。 相似文献
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目的：提升天麻头风灵胶囊质量标准，有效控制其制剂质量。方法：采用薄层色谱法，以紫丁香苷和哈巴俄苷为对照，定性鉴别槲寄生和玄参；采用HPLC法对阿魏酸进行含量测定，C18柱（Inertsil ODS-2；4.6mm×250mm，5μm）；流动相：乙腈-0.05%磷酸溶液（16∶84）；检测波长：321nm；流速：1ml/min；柱温：30℃。结果：薄层色谱定性鉴别方法灵敏，专属性强；阿魏酸在9.97～997ng范围内线性良好，r=0.9999；加样回收率为97.52%。结论：本方法简便、重复性好，为天麻头风灵胶囊质量标准提升奠定了基础。 相似文献
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目的：对益气开胃膏的质量标准进行提高研究。方法：采用TLC薄层色谱技术对益气开胃膏中黄芪、山楂、陈皮等中药进行薄层鉴别，同时采用HPLC高效液相色谱技术对方中黄芪甲苷的含量进行测定。结果：所建立的TLC法能较好地鉴别方中所含的黄芪、山楂、陈皮，斑点对应清晰，阴性无干扰；黄芪甲苷在1.0472～20.9440μg范围内呈良好的线性关系，黄芪甲苷平均回收率为99.50%，RSD为1.32%。结论：所建立的质量控制方法准确、专属性好，能较好地对益气开胃膏进行定性、定量检测，可用于益气开胃膏的质量控制。 相似文献
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目的：建立高效液相色谱（HPLC）法测定疏肝健脾解毒方中毛蕊异黄酮葡萄糖苷的含量。方法：采用HPLC法,以依利特Supersil ODS-C18柱（4.6 mm×250 mm,5 μm）为色谱柱,以乙腈-0.2%甲酸溶液为流动相梯度洗脱（0～23 min,12%乙腈;23～65 min,12%～26%乙腈）,流速为1.0 ml/min,柱温为30℃,检测波长为260 nm。结果：经方法学验证,毛蕊异黄酮葡萄糖苷在7.2～43.2 μg（R2=0.9998）范围内线性关系良好,精密度、稳定性和重复性试验的RSD 均＜2.0%（n=6）,加样回收率为98.87%（RSD为1.79%）。结论：本研究建立的含量测定方法简便,结果准确可靠,适用于疏肝健脾解毒方中毛蕊异黄酮葡萄糖苷的测定,可为其质量标准的深入研究提供依据。 相似文献
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目的：建立三金排石颗粒的质量标准。方法：采用薄层色谱法（TLC）分别对三金排石颗粒中的海金沙、栀子、大黄和山楂共四味中药进行定性研究;采用高效液相色谱法（HPLC）分别对金钱草中的槲皮素和山柰素进行定量研究,色谱柱为C18柱,流动相为甲醇-0.35%磷酸溶液（50∶50）,流速为1.0 ml/min,检测波长为360 nm,柱温为30℃,进样量为10 μl。结果：所建立的TLC法中,海金沙、栀子、大黄和山楂斑点清晰,分离度好,专属性强。槲皮素和山柰素的检测质量浓度线性范围分别在0.010～0.100 mg/ml、0.010～0.101 mg/ml之间（r=0.9993、0.9998,n=6）,精密度、稳定性、重复性试验的RSD均≤2%（n=6）,平均加样回收率分别为101.86%、101.48%（n=6）。结论：本研究所建立的三金排石颗粒的质量标准,TLC法及HPLC法操作简便,准确快速,重现性好,可有效控制三金排石颗粒的质量。 相似文献
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目的：建立护心康片多波长HPLC指纹图谱，并研究复方与组方药物指纹图谱峰的相关性。方法：采用C18色谱柱（4.6mm×200mm，5μm）；柱温35℃；0.1%磷酸溶液（A）-乙腈（B）为流动相梯度洗脱；流速1.0ml/min；检测波长分别为203nm（检测三七和黄芪）、270nm（检测丹参酮类）、286nm（检测丹参酚酸类和黄芪）。结果：建立了23个特征峰的护心康片HPLC指纹图谱，并对其特征峰进行了组方药物归属分析。结论：该方法能揭示复方主要色谱峰在组方药物中的归属，有效控制护心康片的质量。 相似文献
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目的：建立芪芍化纤柔肝汤的质量标准。方法：采用TLC法对制剂中赤芍、黄芪、当归、丹参进行鉴别;采用C18柱,以乙腈-0.1%磷酸溶液为流动相,梯度洗脱,流速为l.0 ml/min,检测波长为230 nm,柱温30℃,测定样品中芍药苷的含量。结果：薄层鉴别的色谱斑点清晰,阴性对照无干扰;芍药苷在0.01701～0.4252 mg/ml浓度范围内具有良好的线性关系,r=0.9998,平均加样回收率为99.37%,RSD为1.22%。结论：该方法操作简便,专属性强,重复性好,可以用于芪芍化纤柔肝汤的质量控制。 相似文献
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目的：通过测定指标性成分含量，比较配方颗粒和全方药材浸膏制备的滋阴温阳活血膏方的临床等效用量之间的差异。方法：含量测定采用高效液相色谱（HPLC）法，使用Phenomex Kinetex C18色谱柱（4.6mm×250mm，5μm），0.1%磷酸-乙腈为流动相，梯度洗脱，流速1.0ml/min，柱温30℃，进样量10μl，检测波长254nm；以腺苷和山奈酚-3-O-β-D-葡萄糖苷为指标性成分，共测定颗粒和浸膏配制的膏方各10批。结果：10批样品中，1g颗粒制备的膏方相当于0.957～1.043g浸膏制备的膏方，二者差异无统计学意义（P＞0.05）。结论：使用配方颗粒可以替代药材浸膏作为制作膏方的材料。 相似文献
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目的：建立测定宁志胶囊中芒果苷含量的HPLC法。方法：采用高效液相色谱法，色谱柱为Agilent 5TC-C18（250 mm×4.6 mm，5 μm），以乙腈-0.2%冰醋酸溶液（13∶87）为流动相，检测波长为258 nm，流速为1.0 ml/min，柱温为30 ℃。结果：芒果苷在4.0951～51.1885 μg/ml之间呈良好线性关系（r=0.99987）。芒果苷平均加样回收率为104.92%（n=6），RSD=1.20%。结论：经方法学验证，本法可用于宁志胶囊的质量控制。 相似文献
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<正>我国是海洋大国,海岛众多。海岛是壮大海洋经济、拓展发展空间的重要依托,是保护海洋环境、维护生态平衡的重要平台,是捍卫国家权益、保障国防安全的战略前沿。2009年12月26 相似文献
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The New Zealand Waters Pollution Regulations 1963, referring to recreational water to which the public can gain access, states that the “coliform bacteria content shall not consistently exceed 1,000/100 ml”. This standard is now under revision, and the suggested new standard is a median faecal coliform index of 200/100 ml with a maximum value not exceeding 400/100 ml. Recent literature supports the use of faecal coliform sub‐group bacteria as the best indicator of faecal pollution and reference is made to the membership of this sub‐group. Inherent inaccuracies of the Most Probable Number (MPN) method of assessing bacterial populations are pointed out, such as experimental variations with different cultural media, and possible errors in interpretation of results. In North America, there is evidence that an MPN for the total coliform group of 1000/100 ml indicates a faecal coliform sub‐group MPN of 200/100 ml, and epidemiological experience supports these values as standards. We tabulate and discuss evidence that the usual ratio of faecal coliforms : coliform group bacteria is typically 1 : 1 in fresh and marine waters of the Wellington region, and that a more attainable standard would be 500 faecal coliforms/100 ml. In ratios quoted in this paper, ‘coliform group’ means the remainder of the coliform group excluding faecal coliforms. The World Health Organisation (1963) definition, of the entire group of coliforms, is not intended. The ratio is different in other parts of New Zealand; in Auckland, for example, the ratio is close to that found in the United States. 相似文献
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《Coastal Engineering》1999,36(3):263-269
Using the logarithmic wind profile and typical values of dimensionless sea roughness for different sea states, simple power expressions are derived for the wind profile over water. The power expressions are useful for correcting measured wind speed data at a given elevation (up to an elevation of 60 m) to the standard elevation of 10 m commonly used in coastal engineering studies. The analysis shows that the 1/7 power expression (suggested in [U.S. Army Corps of Engineers, 1984. Shore Protection Manual, Vol. 1. Coastal Engineering Research Center, Vicksburg, USA.] and commonly used today) is a good approximation (<3% error) of the logarithmic profile for various sea states up to an elevation of 20 m. Above this elevation, the 1/7 power expression underestimates the wind speed for moderate to fully developed sea states. 相似文献
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《Marine Policy》2015
This paper systematically reviews and synthesizes peer-reviewed, English-language scientific publications (n=212) to identify relevant research about how Ecosystem-Based Adaptation (EBA) is integrated with coastal planning. Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) methodology is applied in this study. Attention was given to studies concerning human-environment interactions as opposed to physical or biological climate change issues alone because the coastal planning and EBA approach addresses the management of human actions in nature. The literature references include the issue of climate change (77%); however, limited evidence of EBA in coastal areas are reported (18%), and it is evident that the issues have become relevant in the scientific literature published in recent years. Broad texts demonstrate that SLR is one of the major long-term impacts (68%), and all of these papers recognize the most affected ecosystems in the tropics would be mangroves. EBA is an emerging option that can offset anticipated ecosystem losses and improve coastal planning to cope with SLR because it provides benefits beyond climate change stressors. There is a need to synthesize a road map for incorporation of mangrove regulations into local planning instruments and for building capacity for their implementation. Application of PRISMA in marine science will enhance future reviews, facilitate the systematic search and adequately document any theme, and also be useful in determining research gaps or information needs. 相似文献
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日本在日前发表的《信息通信白皮书》中提出，要建立网络无所不在的社会；美国1999年提出了“21世纪信息技术计划”，由此看出，信息社会正向人类走来，人们唯有通过网络才能快捷、有效地获得有用信息，海洋产业唯有借助网络才能推动科学研究，有效地利用海洋资源，促进海洋经济发展，海洋信息共享工程建设势在必行。标准化原理能确保海洋信息共享工程建设有序、高效、质量优良。 相似文献
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提出了一种用于对不连续压力采样序列的傅立叶分析方法.此方法将周期函数展开成傅立叶级数,但在数值积分时取函数周期内有采样值的区间作为积分域,然后求解线性方程组得到傅立叶级数的系数值.为了检验本方法的有效性,利用此方法对解析函数进行了拟合,当一个周期内的取样时间大于1/2周期时,利用此方法能够得到满意的结果.利用实验方法研究了波浪作用下截断圆柱表面的压力分布.在波浪作用下静水面附近的测点在露出水面时没有压力值.利用所提出的傅立叶分析方法对略低于静水面位置的实测压力进行了分析,拟合结果与实测结果吻合很好,说明此方法在处理物理模型实验中间断采样得到的数据是有效的.利用数值方法对波浪压力进行了计算,并将一阶和二阶波压力的数值结果与实测值进行了比较. 相似文献
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《Marine Policy》2014
This paper summarises research and options for sustainable sea transport in Oceania with a focus on domestic shipping. This debate is situated initially within the context of the current Pacific domestic shipping scenario, a region of minute economies connected by some of the longest sea transport routes in the world. All current options are fossil fuel powered and increasingly uneconomic and unsustainable. Many routes are marginal or unviable and a vicious cycle of old ships replaced with old ships prevails. Although a central and essential issue of many Pacific communities, the option of pursuing sustainable sea transport is currently invisible within the policy space at all levels. Various renewable energy options are possible and increasingly available. Recent research finds that these have strong potential for providing benefits across multiple wellbeings. The barriers to pursing this agenda are complex and poorly understood but are perceptual and institutional more than technological. A small number of critical experiments during the last oil crisis provide critical lessons and direction. 相似文献
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渔港避风锚地面积计算的随机模拟 总被引：1，自引：0，他引：1
计算避风锚地的面积是渔港规划设计的重要内容之一.<渔港总体设计规范>(SC/T 9010-2000)推荐采用确定性方法计算避风锚地面积.由于没有规定输入参数代表值的确定方法,实际应用时参数取值带有很大的随意性和经验性,从而导致计算结果的不确定性.引入Monte Carle方法建立了渔港避风锚地面积计算的随机模拟模型,采用不同的概率分布刻划各相关参数,从而获得避风锚地面积的概率分布.实例计算表明,无论采用何种概率分布假定,得到的避风锚地面积均近似地服从正态分布,且不同概率分布假定对95%保证率的避风锚地面积值影响不大;当船长、船宽均取各分组船型的最大值,水深取相应区域水深的平均值,每组渔船之间安全距离及每组渔船数量取规范给定的平均值时,规范方法计算的避风锚地面积保证率在95%左右,从而为规范方法输入参数的确定提供了依据. 相似文献