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1.
硒对钝顶螺旋藻生长的影响及其在细胞中的累积和分布 总被引：6，自引：0，他引：6
本文研究了硒对纯顶螺旋藻（Spirulinaplatensis）生长的影响及其在细胞生化组成中的分布。结果表明,当硒浓度低于50mg／dm3时,对钝顶螺旋藻生长的抑制作用不明显,在低浓度下（＜10mg／dm3）可促进其生长;当硒浓度超过50mg／dm3时则起抑制作用,高浓度对藻体起毒害作用。藻细胞中总硒含量与培养液中的硒浓度有关,在0～40mg／dm3范围内,硒含量随着培养液中硒浓度的增加而增加：从0mg／dm3的2．50×10-5（m／m,dw）到40mg／dm3的231．45×10-6（m／m,dw）,至50mg／dm3,又开始降低为174.26×10-6（m／m,dw）;硒在S．platensis生化组成中的分布,以蛋白质含量为最高,可达总硒的49．22％～71．49％,而脂类及碳水化合物-氨基酸中的含量则较低,分别为总硒含量的3．59％～6．18％和1．45％～2．56％,硒在各组成中的含量与培养液中的硒浓度有关,其变化趋势与总硒含量的一致。 相似文献
2.
对虾养殖围隔生态系细菌的生物量 总被引：1，自引：0，他引：1
对5个对虾养殖围隔生态系细菌生物量(以C计,下同)的研究结果表明,浮游细菌总生物量波动在0.09～0．83mg/dm3之间,平均0．37±0．21mg／dm3,占水层颗粒有机碳含量的11．94%;附着细菌生物量在(0．14～3．69)x10－2mg/dm3)之间,平均(0．77±0．58)x10-2mg/dm3,占浮游细菌总生物量的2.08%±1．95%。随着养殖时间的推移,浮游细菌总生物量、附着细菌生物量均有所上升。底泥细菌生物量为(44～132)x(10-6m/m),平均(86±23)x(10-6m/m),占底泥有机碳含量的1．55%。 相似文献
3.
浙江舟山群岛邻近海域微,小型浮游植物与赤潮生物研究 总被引：7，自引：0，他引：7
报道了舟山群岛邻近海域未发生赤潮与发生赤潮时的微、小型浮游植物组成、数量分布、赤潮生物种类与环境的关系。舟山群岛邻近海域共有浮游植物 1 69种 ,其中赤潮生物为 59种。赤潮发生时比未发生赤潮时水域的浮游植物密度高 1 0～ 1 0 0个 /dm3,浮游植物密度与硅酸盐、硝酸盐浓度成负相关 ,与溶解氧和透明度成正相关。平均浮游植物密度 :春季表层水样为4.5× 1 0 4 个 /dm3,底层水样为 5.2× 1 0 3个 /dm3,网样为 2 .7× 1 0 5个 /m3;秋季网样为 3.7× 1 0 6个 /m3。表层水样的赤潮生物优势种 :春季为微型的三鳍原甲藻 ,平均细胞密度为 3.3× 1 0 5个/dm3,占总细胞密度的 77.2 % ;秋季为微型的中肋骨条藻 ,平均密度为 2 .7× 1 0 3个 /dm3,占总细胞密度的 72 .2 %。三鳍原甲藻、海洋原甲藻、夜光藻等为浙江舟山群岛邻近海域常见的有害赤潮生物。 相似文献
4.
香鱼工厂化养殖及其耗氧率和窒息点试验 总被引：1，自引：0，他引：1
2 0 0 0年 2～ 3月放养 1 63 3 5尾香鱼 (Plecoglossusaltivelis)种苗 ,在水泥池内进行工厂化养殖研究 .结果表明 ,养殖存活率 85%,总产量 2 4t,平均单产 1 0kg/m2 ,饵料系数 1 .7.养殖主要病害为肠炎和烂鳃病 .体重 2 4.5～ 3 2 .5g ,个体平均耗氧率为(0 .2 98～ 0 .3 86)× 1 0 -3(m/m)h-1.窒息点 1 .95mg/dm3左右 . 相似文献
5.
杭州湾——舟山渔场秋季浮游植物现存量和初级生产力 总被引：8，自引：0，他引：8
1995年 9月在杭州湾和长江口至舟山海区进行了浮游植物细胞丰度、叶绿素a浓度和初级生产力的现场观测研究 .结果表明 ,表层水浮游植物平均细胞丰度为(2 2 6 8± 6 3 33)× 1 0 4个 /dm3;平均叶绿素a浓度为 2 80± 3 46 μg/dm3,小于 2 0μm的微型和微微型浮游生物细胞对叶绿素a的贡献占 71 % ;平均初级生产力 (C)为6 92 5± 1 1 92 4mg/ (m2 ·d) ,小于 2 0 μm的微型和微微型浮游生物细胞对总生产力的贡献占 6 8% .河口区悬浮物质浓度高 ,浮游植物光合作用受光的限制 ,各项生物参数与真光层深度紧密相关 .生物锋区位于真光层深度 1 0～ 2 0m、盐度 2 6～ 32的长江冲淡水稀释区 .同时探讨了浮游植物细胞活性 (R)与光合作用同化数 (AN)、叶绿素a与初级生产力、叶绿素a与海面光谱反射率的相互关系 ,为海洋水色遥感在初级生产力的应用研究提供科学依据 相似文献
6.
5-羟色胺刺激长牡蛎排放精卵在四倍体诱导中的应用 总被引：4，自引：0，他引：4
本文报道了用 5 羟色胺催产出的长牡蛎精、卵 ,人工授精后诱导四倍体的实验结果 .结果表明 :在 2 6℃水温条件下 ,受精后 7min ,用 0 .5mg/dm3细胞松弛素B处理2 5min ,获得长牡蛎的胚胎四倍体 ,其诱导率为 1 6.7%,D形幼虫孵化率为 2 7.0 %;诱导效果优于采用常规解剖法 .注射 5 羟色胺刺激长牡蛎亲贝排放精、卵的有效浓度分别为 1× 1 0 -6 ～ 1× 1 0 -2 mol/dm3和 1× 1 0 -5 ～ 1× 1 0 -3mol/dm3. 相似文献
7.
3种植物激素对螺旋藻生长和代谢产物含量的影响 总被引：1，自引：0，他引：1
用单因素分析法研究6一苄基氨基嘌呤(6-BA)、萘乙酸(NAA)和赤霉素(GA)3种植物激素对螺旋藻生长和代谢产物含量的影响.结果显示,添加NAA(0.4 mg/L)和GA(1.0 mg/L)分别使钝顶螺旋藻(Speruli-na pzatensis)和极大螺旋藻(Spirulina maximum)生物量达到最大值,与对照相比提高了49.1%和65.2%,比生长速率增加了66.7%和28.6%.同样,激素可以显著地影响螺旋藻的代谢产物含量.对于钝顶螺旋藻,GA(0.1 mg/L)使胞外总糖含量增加5倍;GA(0.3 mg/L)使胞内总糖含量提高5倍;6-BA(0.2 mg/L)使胞内蛋白含量提高27.8%;NAA(0.2 111g/L)使SOD酶比活力提高3.47倍.对于极大螺旋藻,GA(0.3 nlg/L)使胞外总糖含量增加1.32倍,使胞内总糖含量提高2倍;NAA(1.6mg/L)使胞内蛋白含量提高66.7%,6-BA(1.6 mg/L)使SOD酶比活力提高3.47倍.因此植物激素对螺旋藻生长和代谢产物合成有明显的促进作用,该结果为高密度规模化生产奠定基础. 相似文献
8.
北部湾浮游植物粒径分级叶绿素a和初级生产力的分布特征 总被引：19，自引：4，他引：19
1994年5月23B至6月4日现场观测了北部湾浮游植物细胞丰度、叶绿素a浓度和初级生产力的分布.测区平均叶绿素a浓度为0.94±0.45/d3.平均初级生产力(C)为351±172mg/(m2·d),浮游植物细胞丰度为0.97×104-10050×104个/m3,鉴定浮游植物4门56属176种.地理环境和水文状况的差异使上述参数分布具有明显的区域性特征,近岸区高于湾中部,测区北部高干南部;温跃层以下水层叶绿素a浓度高于上层水,周6观测站平均叶绿素a浓度湾北部(0.47±0.15g/dm3)高于湾南部(0.15±0.02dm3).北部湾水域光合浮游生物以微型和微微型细胞(小于20m)占优势,其对总初级生产力的贡献(占91%)高于对总叶绿素a的贡献(占77%). 相似文献
9.
长江口及其毗连东海水域蓝细菌的分布和细胞特性及其环境调节 总被引：18，自引：6，他引：18
1986年1月和7月,作者在长江口及冲淡水区对海洋蓝细菌Synechococcus spp.的密度分布,细胞特性及其与环境要素的关系进行了研究.结果表明,冬季细胞密度低(平均约103cell/cm3),活性也低.夏季细胞密度要比冬季高1至2个数量级.高值区出现在离长江口门向东大约100km的带状区,在该区细胞密度峰值(2×105cell/cm3)的出现是由于富营养盐的长江冲淡水,随着悬浮物质的沉降(临界值为2-3mg/dm3),光的利用率增加的结果.流动细胞仪测定结果表明,细胞密度最大区,平均细胞体积最小,这与种群生长速率高有关;细胞藻红蛋白的含量受光的利用率和无机氮的浓度所调节. 相似文献
10.
胶州湾浮游植物数量长期动态变化的研究 总被引：32，自引：14，他引：18
应用分光光度法对 1 991— 2 0 0 2年 1 1月胶州湾浮游植物现存量 (叶绿素a含量 )进行了测定。研究结果表明 ,胶州湾叶绿素a年平均含量为 2 0 9— 5 70mg/m3,多年平均为 ( 3 47± 1 92 )mg/m3,年际间存在一定的波动范围 ;在平面分布上 ,胶州湾西北和东北近岸海域含量较高 ,湾中部和南部海域较低 ;胶州湾叶绿素a含量存在着明显的季节变化 ,冬季 ( 2月 )平均含量为 ( 4 72± 3 1 5 )mg/m3,是一年中的高峰 ,夏季次之 ,平均含量为 ( 4 33± 2 5 7)mg/m3,春季平均含量为 ( 2 78± 2 43)mg/m3,秋季平均含量最低 ,仅为 ( 1 95± 0 80 )mg/m3。胶州湾浮游植物粒级构成为 :小型浮游植物 ( >2 0 μm)平均占 35 8% ,微型浮游植物 ( 2—2 0 μm)平均为 5 1 3% ,超微型浮游植物 ( <2 μm)平均为 1 2 9% ;不同季节和海域粒级构成有一定差异。 相似文献
11.
Chandralata Raghukumar G. Sheelu P. A. Loka Bharathi Shanta Nair C. Mohandass 《Marine Georesources & Geotechnology》2001,19(1):1-16
In order to assess the impact of deep-sea mining on the in situ benthic life, we measured the microbial standing stock and concentration of organic nutrients in the deep-sea sediments of the Central Indian Ocean Basin in the Indian pioneer area. Sediments were collected using box core and grab samples during September 1996. The total bacterial numbers ranged from 10 10 -10 11 cells per g -1 dry weight sediment. There was a marginal decrease in the number of bacteria from surface to 30 cm depth, though the subsurface section registered a higher number than did the surface. The highest numbers were encountered at depths of 4-8 cm. The retrievable number of bacteria were two orders less in comparison with the direct total counts of bacteria. An almost homogeneous distribution of bacteria, total organic carbon, living biomass, and lipids throughout the depth of cores indicates active microbial and benthic processes in the deep sea sediments. On the other hand, a uniform distribution of total counts of bacteria, carbohydrates, and total organic carbon in all the cores indicates their stable nature and suggests that they can serve as useful parameters for long-term monitoring of the area after the benthic disturbance. Further studies on temporal variability in this region would not only verify the observed norms of distribution of these variables but would also help to understand restabilization processes after the simulated benthic disturbance. 相似文献
12.
Arne Schnack-Friedrichsen Angela M. Davis Jim D. Bennell Dei G. Huws 《Marine Georesources & Geotechnology》2001,19(4):221-243
The interdependence between the seismo-acoustic properties of a marine sediment and its geotechnical/physical parameters has been known for many years, and it has been postulated that this should allow the extraction of geotechnical information from seismic data. Though in the literature many correlations have been published for the surficial layer, there is a lack of information for greater sediment depths. In this article, a desktop study on a synthetic seafloor model illustrates how the application of published near-surface prediction equations to subsurface sediments (up to several tens of meters burial depth) can lead to spurious predictions. To test this further, acoustic and geotechnical properties were measured on a number of sediment core samples, some of which were subjected to loading in acoustically-equipped consolidation cells (oedometers) to simulate greater burial depth conditions. For low effective pressures (representing small burial depths extending to around 10 meters subsurface), the general applicability of established relationships was confirmed: the prediction of porosity, bulk density, and mean grain size from acoustic velocity and impedance appears generally possible for the investigated sedimentary environments. As effective pressure increases through, the observed relationships deviate more and more from the established ones for the near-surface area. For the samples tested in this study, in some instances increasing pressure even resulted in decreasing velocities. There are several possible explanations for this abnormal behavior, including the presence of gas, overconsolidation, or bimodal grain size distribution. The results indicate that an appropriate depth correction must be introduced into the published prediction equations in order to obtain reliable estimates of physical sediment properties for greater subsurface depths. 相似文献
13.
Geoacoustic Inversion via Genetic Algorithm and Its Application to Manganese Sediment Identification
An acoustic inversion method using a wide-band signal and two near field receivers is proposed and applied to multiple layered seabed models including a manganese sediment. The inversion problem can be formulated into a probabilistic model comprised of signals, a forward model, and additive noise. The forward model simulates wide-band signals, such as chirp signals, and is chosen to be the source-waveletconvolution plane wave modeling method. The wavelet matching technique, using weighted least-squares fitting, estimates the sediment sound-speed and thickness on which determination of the possible numerical ranges for a priori uniform distribution is based. The genetic algorithm is applied to a global optimization problem to find a maximum a posteriori solution for determined a priori search space. Here the object function is defined by an L 2 norm of the difference between measured and modeled signals. Not only the marginal pdf but also its statistics are calculated by numerical evaluation of integrals using the samples selected during importance sampling process of the genetic algorithm. 相似文献
14.
海上大直径钢管桩打桩过程中,桩周土体受到强烈扰动而发生强度弱化,掌握桩周土体强度弱化规律对于准确预测打桩过程、保证工程安全具有重要意义。为研究土体强度弱化规律,开展了环剪试验模拟打桩对桩周土体的扰动,测试土体强度随剪切速率的变化规律,建立了描述土体强度弱化规律的拟合公式,引入到打桩分析软件中。研究结果表明：土体的强度折减程度不仅与土体本身的性质有关还受到土体的埋深和剪切速率的影响,埋深越深土体强度折减程度越低,剪切速率越高土体强度折减越高,在打桩分析中可采用这里推荐的线性折减方法来模拟不同深度处土体强度的折减规律。 相似文献
15.
Shigehisa Nakamura 《Marine Geodesy》2000,23(1):55-61
Specific properties of the interannual sea level variations and annual tides in the Northwestern Pacific were studied. Several tide stations were monitored. The monthly mean sea level for the year of 1995 was analyzed at each tide station. A seismic event in 1995, some tectonic activity around the subject area, and the Kuroshio (the oceanic western boundary current) may possibly contaminate results which would have occurred from the astronomical annual tide alone. 相似文献
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Oedometer tests have been carried out on 70 undisturbed surficial clays (at approximately 250 mm below the mudline), mostly collected by free-fall corers from sites widely scattered throughout the deep-sea North Atlantic. Acoustic measurements were also made, initially on contiguous samples and ultimately on the same sample using a geophysically instrumented oedometer which also collected electrical resistivity data. Apart from those quiescent areas below the carbonate compensation depth, such as north of the West Indies where very fine clays exist, most of the samples are silty clays whose geotechnical-geophysical properties are dependent on the type of clay minerals present (and their ability to take in moisture), the sand-size fraction, and the quantity of carbonate present. Thus the pure clays have high compressibilities which decrease on the addition of coarse particles, while the converse is true for the acoustic parameters, these increasing with the sand fraction. Using the notion of the intrinsic compression line for all samples, and comparison to it of the measured compression curves, it is clear that, contrary to some previously held ideas, most deep-sea clays are normally consolidated; the addition of carbonate has the effect of creating an open, stronger sediment skeleton. Interestingly, where information is available, the variation with depth of a sample's acoustic velocity follows the void ratio pressure relationship of the compression curve. This allows the construction of an in-situ sediment compression curve using the in-situ geophysical observations. 相似文献
18.
The effect of different fixation and storage protocols on the flow cytometric (FCM) simultaneous analysis of bacterioplankton and phytoplankton in coastal seawater samples (Mediterranean coastal lagoons) was investigated. FCM measurements (cell number, fluorescence and scatter characteristics) were obtained through DAPI staining. Three fixatives [glutaraldehyde (GA), formaldehyde (FA) and paraformaldehyde (PFA)] and two storage (3 months duration) methods (5 °C and −196 °C) were tested. Two dominant populations were detected in studied samples: bacteria and eukaryotic picophytoplankton. Adding fixatives (2% final concentration) appears necessary to obtain FCM exhaustive counts of all the bacteria and phytoplanktonic cells. This was related to the permeation effect of fixatives which allowed a better DAPI staining of the cells. Maximum fluorescence, i.e. optimal staining of the cells was obtained with FA or PFA, and significant lower fluorescences with GA. Fixed samples stored at 5 °C induced rapid cell loss. Only storage in liquid nitrogen of samples fixed with FA or PFA, allows mid-term (≥4 months) preservation of bacteria or picophytoplankton cell numbers, and limited evolution of DAPI-induced fluorescence and scatter characteristics. 相似文献
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Phylogenetic diversity and phenotypic characterization of cultivable bacterioplankton isolated from polar oceans 总被引：1，自引：0，他引：1
A set of 27 marine planktonic bacteria isolated from the polar regions was characterized by 16S rDNA sequencing and physiological and biochemical testing. More than half of these bacteria were positive for caseinase, gelatinase and 13-glucosidase, and could utilize glucose, maltose or malic acid as carbon source for cell growth. Twelve isolates expressed nitrate reduction activities. Except for one antarctic isolate BSwlO175 belonging to Actinobacteria phylum, these isolates were classified as γ-Proteobacteria, suggesting that γ-Proteobacteria dominated in cultivable marine bacterioplankton at both poles. Genus Pseudoalteromonas was the predominant group in the Chukchi Sea and the Bering Sea, and genus ShewaneUa dominated in cultivable bacterioplankton in the Prydz Bay. With sequence similarities above 97%, genus Psychrobacter was found at both poles. These 27 isolates were psychrotolerant, and significant 16S rDNA sequence similarities were found not only between arctic and antarctic marine bacteria （ 〉 99% ）, but also between polar marine bacteria and bacteria from other aquatic environments （ ≥ 98.8% ）, including temperate ocean, deep sea, pond and lake, suggesting that in the polar oceans less temperature-sensitive bacteria may be cosmopolitan and have a bipolar, even global, distribution at the species level. 相似文献