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1.
A single bioaugmentation reactor and a side-stream gaslift membrane bioreactor combined with bioaugmentation are conducted to treat real wastewater from a centralized piggery slaughterhouse in Vietnam. The bioaugmented reactor is inoculated with heterotrophic microorganisms (Bacillus sp.) isolated from piggery slaughterhouse wastewater. The results of a single bioaugmentation experiment show high removal efficiency of chemical oxygen demand (COD) (84.8%–97.5%) and total nitrogen (TN) (69.9%–87.2%) at loading rates of 1.28–3.89 and 0.14–0.37 kg m−3 d−1, respectively. The combined system demonstrates a significantly higher TN removal efficiency (89.0%–96.1%) (p < 0.001), more stable flux (36.0–38.4 L m−2 h−1), and transmembrane pressure (0.95–1.05 bar), and better capacity of separation of solid–liquid phases compared to the single bioaugmentation. High COD and TN removal efficiency is possibly due to assimilation and simultaneous nitrification and denitrification processes. The results of this study also indicate the feasibility and propitious efficiency of the bioaugmented gaslift membrane bioreactor for piggery slaughterhouse wastewater treatment.  相似文献   

2.
Aerobic granular sludge was successfully cultivated in a sequencing batch reactor (SBR) treating wastewater from the malting process with a high content of particulate organic matter. At an organic loading rate of 3.2 kg/(m3 d) CODtotal and an influent particle concentration of 0.95 g/L MLSS an average removal of 50% in CODtotal and 80% in CODdissolved could be achieved. A comparison of granular and flocculent sludge grown under the same operating conditions showed no significant difference in removal efficiency although granules exhibited a higher metabolic activity in terms of specific oxygen uptake rate (rO2, X). Two distinct mechanisms of particle removal were observed for granular sludge: during initial granule formation, particles were incorporated into the biofilm matrix. For mature granules, a high level of protozoa growth on the granule surface accounted for the ability to remove particulate COD. Combined evaluation of the development in MLSS content and sludge bed settling rate (i.e., mean derivative of the normalized sludge volume) was found to be an adequate method for monitoring the characteristic settling properties of a granulizing sludge bed. By means of this method, a distinct substrate gradient out of several operating conditions was concluded to have the biggest impact on the formation of aerobic granular sludge.  相似文献   

3.
Oil refinery wastewater was treated using a coupled treatment process including electrocoagulation (EC) and a fixed film aerobic bioreactor. Different variables were tested to identify the best conditions using this procedure. After EC, the effluent was treated in an aerobic biofilter. EC was capable to remove over 88% of the overall chemical oxygen demand (COD) in the wastewater under the best working conditions (6.5 V, 0.1 M NaCl, 4 electrodes without initial pH adjustment) with total petroleum hydrocarbon (TPH) removal slightly higher than 80%. Aluminum release from the electrodes to the wastewater was found an important factor for the EC efficiency and closely related with several operational factors. Application of EC allowed to increase the biodegradability of the sample from 0.015, rated as non-biodegradable, up to 0.5 widely considered as biodegradable. The effluent was further treated using an aerobic biofilter inoculated with a bacterial consortium including gram positive and gram negative strains and tested for COD and TPH removal from the EC treated effluent during 30 days. Cell count showed the typical bacteria growth starting at day three and increasing up to a maximum after eight days. After day eight, cell growth showed a plateau which agreed with the highest decrease on contaminant concentration. Final TPHs concentration was found about 600 mgL−1 after 30 days whereas COD concentration after biological treatment was as low as 933 mgL−1. The coupled EC-aerobic biofilter was capable to remove up to 98% of the total TPH amount and over 95% of the COD load in the oil refinery wastewater.  相似文献   

4.
This study focused on removal of color and chemical oxygen demand (COD) parameter from textile effluents using a static granular bed reactor (SGBR), which has never been used to treat textile effluents previously. With an organic loading rate (OLR) of 1 kg/m3 day and a hydraulic retention time (HRT) of 48 h, COD and color removal efficiencies were 74 and 61%, respectively, while the removal efficiencies were 72 and 57%, respectively, with OLR of 1.7 kg/(m3 day) and HRT of 24 h. It was concluded that the SGBR could be used as an alternative method to treat and decolorize textile effluents. First order and modified Stover–Kincannon models were used to develop a kinetic model using the experimental data with correlation coefficients (R2) of 0.39 and 0.94, respectively. In regard with the calculated correlation coefficients, modified Stover–Kincannon model, which was used to model anerobic biofilters in previous studies, fitted best with the experimental data and it was stated that SGBR worked as an anerobic biofilter.  相似文献   

5.
Selenium (Se) may cause reproductive toxicity, yet the characteristics of Se bioaccumulation in aquatic food webs are understudied. Stream biofilms were grown in two reaches of Mud River, West Virginia (WV), including one downstream of a coal mine complex and an adjacent, unmined watershed. Mined stream biofilms contained significantly higher Se concentrations compared to unmined biofilms. An inverse relationship between water Se concentrations and biofilm accumulation factors was observed; mined-stream biofilms had an average bioconcentration factor (BCF) of 688 ± 350 fold while unmined-stream biofilms had an average BCF of 14505 ± 2700 fold.  相似文献   

6.
Glucose‐fed high‐rate UASB reactors were tested at three COD/SO4 ratios and hydraulic retention times to promote sulfate reducing activity and observe the effects on reactor performance. Different COD/SO4 ratios (20, 10, and 5) resulted in changes in organic matter removal, methane production, alkalinity, dissolved sulfide and biomass concentrations and profile. The COD removal dropped from 95 to 80–84 % at the lowest COD/SO4 ratio. Sulfate was removed at 79 to 89 % at the highest ratio and dropped to 72 to 74 % with increasing sulfate loading. Alkalinity was produced at higher levels with increasing sulfate loading. Specific methane production dropped with decreasing hydraulic retention times. Sulfate‐reducing activity used a maximum of 11.7 % of organic matter at the highest sulfate loading level, producing a slight shift to sulfate‐reducing activity in the substrate competition between sulfate‐reducing bacteria and methanogens. Increased sulfate loading at COD/SO4 ratios of 10 and 5 caused deterioration of the concentration profile of the sludge, resulting in biomass washout and decreased volatile fraction of biosolids in the reactors.  相似文献   

7.
The microbiological assessment of biofilm formation from paper mill effluent discharged through a pipeline revealed a maximum microbial count for Pseudomonas (5·106 cfu/mL) followed by Staphylococcus (4·106 cfu/mL), Bacillus (8.2·105 cfu/mL), Burkholderia (7.2·105 cfu/mL), Enterobacter (5.3·104 cfu/mL), Acinetobacter (4.1·103 cfu/mL), Alcaligenes (1.2·102 cfu/mL) and Klebsiella (0.8·102 cfu/mL) species. Among these species, the maximum biofilm formation was observed after 24 h of incubation by Pseudomonas sp. using a crystal violet (CV) assay. This isolate was later identified by 16S rRNA amplification to be Pseudomonas aeruginosa PME1. Extracellular polymeric substances (EPS) of P. aeruginosa PME1 in the biofilm showed a reduction in total carbohydrate content (42%) with increased protein (9.0%), hexosamine (3.0%) and uronic acid (1.7%) content as compared to its planktonic form. Antimicrobial susceptibility testing revealed that P. aeruginosa PME1 biofilms were 17, 24, 27, 30, and 32 times more resistant to cefotaxime, imipenem, ceftazidime, tazobactam and piperacillin, respectively, than their free flowing counterparts.  相似文献   

8.
Groundwater denitrification was carried out in a fluidized bed reactor. This type of reactor is suited well to conditions of low substrate concentrations and high flow rates which are typical in groundwater denitrification processes. The reactor is characterized by the efficient contact between biomass and substrate, a high biomass concentration, and the absence of clogging or channeling. These characteristics provide for high treatment capacities (10.8 g/(L·d) NO3?N) and short retention times (less than 3 minutes) resulting in small reactor volume. Start-up procedure, biomass and nitrate profiles, and biofilm characteristics are presented in this article.  相似文献   

9.
This research addresses the challenges of the lack of non‐invasive methods and poor spatiotemporal resolution associated with monitoring biogeochemical activity central to bioremediation of subsurface contaminants. Remediation efforts often include growth of biofilm to contain or degrade chemical contaminants, such as nitrates, hydrocarbons, heavy metals, and some chlorinated solvents. Previous research indicates that nuclear magnetic resonance (NMR) is sensitive to the biogeochemical processes of biofilm accumulation. The current research focuses on developing methods to use low‐cost NMR technology to support in situ monitoring of biofilm growth and geochemical remediation processes in the subsurface. Biofilm was grown in a lab‐scale radial flow bioreactor designed to model the near wellbore subsurface environment. The Vista Clara Javelin NMR logging device, a slim down‐the‐borehole probe, collected NMR measurements over the course of eight days while biofilm was cultivated in the sand‐packed reactor. Measured NMR mean log T2 relaxation times decreased from approximately 710 to 389 ms, indicating that the pore environment and bulk fluid properties were changing due to biofilm growth. Destructive sampling employing drop plate microbial population analysis and scanning electron and stereoscopic microscopy confirmed biofilm formation. Our findings demonstrate that the NMR logging tool can detect small to moderate changes in T2 distribution associated with environmentally relevant quantities of biofilm in quartz sand.  相似文献   

10.
The objective of this study was to investigate the effect of salt concentration on performance of a membrane bioreactor (MBR) for treating an olefin plant wastewater. For this purpose, a lab‐scale submerged MBR with a flat‐sheet ultrafiltration membrane was used for treatment of synthetic wastewater according to oxidation and neutralization unit of olefin plant. The synthetic wastewater was adjusted to have 500 mg/L chemical oxygen demand (COD). Trials on different concentrations of sodium sulfate (Na2SO4) (0–20 000 ppm) in the feed were conducted under aerobic conditions in the MBR. The results showed that increasing the salt concentrations causes an increase in the effluent COD, phenol, and oil concentrations. These results are due to reduction of the membrane filtration efficiency and also decline in the microbial activity that it is indicated by decreasing the sOUR in MBR. But in all the trials, the effluent COD and oil concentration was well within the local discharge limit of 100 and 10 mg/L, respectively. These results indicate that the MBR system is highly efficient for treating the olefin plant wastewater, and although high salt concentrations decreased organic contaminant removal rates in the MBR, the effluent still met the discharge limits for treating the olefin plant wastewater.  相似文献   

11.
A start‐up study for biohydrogen production from palm oil mill effluent (POME) is carried out in a pilot‐scale up‐flow anaerobic sludge blanket fixed‐film reactor (UASFF). A substrate with a chemical oxygen demand (COD) of 30 g L?1 is used, starting with molasses solution for 30 days and followed by a 10% v/v increment of POME/molasses ratio. At 100% POME, a hydrogen content of 80%, hydrogen production rate of 36 L H2 per day, and maximum COD removal of 48.7% are achieved. Bio‐kinetic coefficients of Monod, first‐order, Grau second‐order, and Stover‐Kincannon kinetic models are calculated to describe the performance of the system. The steady‐state data with 100% POME shows that Monod and Stover‐Kincannon models with bio‐kinetic coefficients of half‐velocity constant (Ks) of 6000 mg COD L?1, microbial decay rate (Kd) of 0.0015 per day, growth yield constant (Y) of 0.786 mg volatile suspended solids (VSS)/mg COD, specific biomass growth rate (μmax) of 0.568 per day, and substrate consumption rate of (Umax) 3.98 g/L day could be considered as superior models with correlation coefficients (R2) of 0.918 and 0.989, respectively, compared to first‐order and Grau's second‐order models with coefficients of K1 1.08 per day, R2 0.739, and K2s 1.69 per day, a = 7.0 per day, b = 0.847.  相似文献   

12.
The aim of this study is to evaluate the feasibility of sludge digester effluent as feeding solution to enrich anaerobic ammonium oxidation (anammox) bacteria. The performance of the two parallel pilot scale‐upflow packed bed anammox reactors (UPBAn1 and UPBAn2) are examined in terms of the enrichment of anammox bacteria. The control experiment is set up conducting synthetic wastewater as feeding solution in the UPBAn1 reactor whereas, the sludge digester effluent is fed to the nitritation reactor and then the partially nitrated digester effluent to the UPBAn2 reactor. Anammox activities are evaluated by mass balances based on ammonium (NH4+), nitrite (NO2?), and nitrate (NO3?) analysis and NRR. Microbial community of anammox bacteria is analyzed using real‐time polymerase chain reaction (PCR). The results demonstrate that UPBAn 1 and UPBAn2 reactors are successfully enriched on days 64 and 40 with NRRs of 19.54 and 19.43 g N m?3 per day, respectively. This study reveals that both synthetic wastewater and digester effluent are suitable for the enrichment of anammox bacteria; however, digester effluent as feeding solution for enrichment of anammox bacteria based on the ease of process control and process stability is more advisable.  相似文献   

13.
Soil erosion in sloping cropland is a key water and soil conservation issue in the Loess Plateau region, China. How surface roughness influences soil detachment remains unclear due to the inconsistent results obtained from existing studies. The objectives of the present study were to evaluate the effects of tillage practices on soil detachment rate in sloping cropland and establish an accurate empirical model for the prediction of soil detachment rates. A series of movable bed experiments were conducted on sloping surfaces under three different tillage practices (manual dibbling, manual hoeing, and contour drilling), with a smooth surface (non-tillage) as a control. The research indicated that soil detachment rate significantly increased with roughness (p < 0.05) since the average soil detachment rate was the highest under the contour drilling treatment (6.762 g m−2 s−1), followed by manual hoeing (4.180 g m−2 s−1), and manual dibbling (3.334 g m−2 s−1); the lowest detachment rate was observed under the non-tillage treatment (3.214 g m−2 s−1). Slope gradient and unit discharge rate were positively correlated with soil detachment rate and proved to be more influential than soil surface roughness. Four composite hydraulic parameters were introduced to estimate soil detachment rate on tilled surfaces. Regression analyses revealed that stream power was the most effective predictor of soil detachment rate compared with unit length shear force, shear stress, and unit stream power. By integrating surface roughness as a variable, the detachment rate could be accurately described as a nonlinear function of stream power and surface roughness. The results of the present study indicate that tillage practice could influence soil loss on sloping cropland, considering the higher soil detachment rates under all tillage practices tested compared with non-tillage. The results are attributed mainly to concentrated flow caused by the high water storage levels on tilled surfaces, which could damage surface microtopography and, subsequently, the development of headcuts.  相似文献   

14.
To evaluate the performance of Spirodela polyrrhiza grown in swine wastewater for protein and starch production under field conditions, a pilot‐scale duckweed culture pond was installed at Barham Farm, Zebulon, North Carolina and operated from May to November 2010. The anaerobically treated swine wastewater was fed to the duckweed pond intermittently to provide nutrients for the growth of duckweed, and the duckweed biomass was harvested regularly from the pond and prepared as a protein‐ or starch‐rich feedstock for the production of animal feed or fuel ethanol. Over the experimental period, the duckweed pond produced protein and starch at rates of 2.68 and 1.88 g m?2 day?1, respectively. During the same time, NH4–N and o‐PO4–P in the wastewater were, respectively, removed at rates of 92.9 and 2.90 mmol m?2 day?1.  相似文献   

15.
To elucidate splash erosion processes under natural rainfall conditions, temporal variations in splash detachment were observed using a piezoelectric saltation sensor (H11B; Sensit Co., Portland, ND, USA). Preliminary laboratory tests of Sensit suggested that they were suitable for field observations. Field observations were conducted between July and September 2006 in 21‐ and 36‐year‐old Japanese cypress (Chamaecyparis obtusa) plantations with mean stand heights of 9·2 m and 17·4 m, respectively. Splash detachment (in g m?2) was measured seven times using splash cups, and raindrop kinetic energy (in J m?2 mm?1) in both stands was measured using laser drop‐sizing (LD) gauges. Sensit was installed to record saltation counts, which were converted to temporal data of splash detachment (splash rate; in g m?2 10 min?1) using the relationship between splash detachment and saltation counts. Surface runoff was monitored using runoff plots of 0·5 m width and 2·0 m length to obtain temporal data of flow depth (in millimeters). Both total splash detachment and raindrop kinetic energy were larger in the older stand. Increased splash rates per unit throughfall were found in both stands after rainless durations longer than approximately one day in both stands. However, a lower splash rate was found in the 21‐year stand after rainfall events. During extreme rainstorms, the 21‐year stand showed a low runoff rate and a decline in the splash rate, while the 36‐year stand showed a higher splash rate and increased flow depth. The piezoelectric sensor proved to be a useful means to elucidate splash erosion processes in field conditions. Copyright © 2010 John Wiley & Sons, Ltd.  相似文献   

16.
In the present study, chemical oxygen demand (COD) removal by packed‐columns of activated carbon (AC) derived from two different materials (coal activated carbon, CAC and wood activated carbon, WAC) is reported as part of an on‐site wastewater treatment system for handling small volumes of wastewater generated at wood‐floor industries for which there are no proper on‐site treatment options available in the market. The performance of the sorbents, the effect of bed depth (0.19 and 0.57 m) and volumetric load (0.10 and 0.24 m h?1) on the breakthrough curve of sorption systems were studied. The results indicated the feasibility of using both ACs to treat these wastewaters. At the bed depth (0.57 m), volumetric load (0.24 m h?1), and 30% breakthrough, CAC and WAC showed treatment capacity of 40.5 L kg?1 in 250 h and 23.8 L kg?1 in 63 h, respectively. This indicated that CAC requires longer retention times to reach a performance similar to WAC. The experimental data was fit into the bed depth‐service time model showing that under the same conditions, CAC had higher maximum sorption capacity (N0) than WAC. Moreover, thermal regeneration at 500°C temperature could be a cost‐effective procedure since the reuse of spent AC through such regeneration process for further treatment could still achieve 90% of the initial sorption capacity, reducing then costs for the use of new sorbents and also the need for waste disposal.  相似文献   

17.
This study investigated the effectiveness of a new packing material, namely mixed rice husk silica with dried activated sludge for removing H2S. Dried sewage sludge was collected from Putrajaya sewage treatment plant in Malaysia. Rice husk silica was prepared at temperature of 800°C, after acid leaching and mixed with dried sewage sludge to be utilized in a polyvinyl chloride filter. The system was operated under variable conditions of two parameters, different inlet gas concentration and different inlet flow rate. H2S was passed through the filter with one liter of the packing material. More than 99.96% removal efficiency (RE) with empty bed residence time (EBRT) of 90–45 s and 300 ppm inlet concentration was observed. However, the RE decreased to 96.87% with the EBRT of 30 s. The maximum elimination capacity (EC) of 52.32 g/m3/h was obtained with the RE of 96.87% and H2S mass loading rate of 54 g/m3/h, while at the RE of 99.96%, maximum EC was 26.99 g/m3/h with the H2S mass‐loading rate of 27 g/m3/h. A strong significant correlation between increasing of H2S mass loading rate and pressure drop was also detected (p < 0.01). Maximum pressure drop was 3.0 mm H2O after 53 days of operating time, the EBRT of 30 s, and 54 g/m3/h of H2S loading rate. These observations suggest that the mixture of rice husk silica with dried activated sludge is a suitable physico‐biological filter for H2S removal.  相似文献   

18.
In the present study, the effects of initial COD (chemical oxygen demand), initial pH, Fe2+/H2O2 molar ratio and UV contact time on COD removal from medium density fiberboard (MDF) wastewater using photo‐assisted Fenton oxidation treatment were investigated. In order to optimize the removal efficiency, batch operations were carried out. The influence of the aforementioned parameters on COD removal efficiency was studied using response surface methodology (RSM). The optimal conditions for maximum COD removal efficiency from MDF wastewater under experimental conditions were obtained at initial COD of 4000 mg/L, Fe2+/H2O2 molar ratio of 0.11, initial solution pH of 6.5 and UV contact time of 70 min. The obtained results for maximum COD removal efficiency of 96% revealed that photo‐assisted Fenton oxidation is very effective for treating MDF wastewater.  相似文献   

19.
Non‐uniform distribution of biomass and the accumulation of excess biomass at the inlet of a unidirectional flow biotrickling filter (UF‐BTF) may lead to severe operating problems including increasing airflow resistance in packed bed, clogging, and low contaminant removal efficiencies, even performance loss. Therefore, a flow‐directional‐switching biotrickling filter (FDS‐BTF) was designed to improve performance such as the maximum elimination capacity and elimination efficiency. Toluene was chosen as the unique carbon source for microorganism. The results indicated that the purification performance of FDS‐BTF was superior to UF‐BTF. The maximum elimination capacity of FDS‐BTF was up to 480 g/(m3 h), which was 17.1% higher than that of UF‐BTF. After a 50‐day continuous operation, FDS‐BTF attained the stable purification performance and the outlet concentrations of toluene could meet the emission standard with inlet concentrations ranging from 720 to 1100 mg/m3 for an empty bed residence time (EBRT) of 23.9 s. The average well color development in FDS‐BTF was higher than that in UF‐BTF. It indicated that FDS‐BTF could improve the metabolic activity, which may improve the uniform distribution of biomass along the length of packed bed. When two systems were shut down, 24 and 48 h, respectively, the time that FDS‐BTF and UF‐BTF needed to restore the former elimination efficiency after a shut down of 48 h were 3–4 and 9–10 h. These data indicated that the purification performance of FDS‐BTF was superior to UF‐BTF.  相似文献   

20.
This study reports the performance of a combined anaerobic–aerobic packed‐bed reactor that can be used to treat domestic sewage. Initially, a bench‐scale reactor was operated in three experimental phases. In the first phase, the anaerobic reactor was operated with an average organic matter removal efficiency of 77% for a hydraulic retention time (HRT) of 10 h. In the second phase, the reactor was operated with an anaerobic stage followed by an aerobic zone, resulting in a mean value of 91% efficiency. In the third and final phase, the anaerobic–aerobic reactor was operated with recirculation of the effluent of the reactor through the anaerobic zone. The system yielded mean total nitrogen removal percentages of 65 and 75% for recycle ratios (r) of 0.5 and 1.5, respectively, and the chemical oxygen demand (COD) removal efficiencies were higher than 90%. When the pilot‐scale reactor was operated with an HRT of 12 h and r values of 1.5 and 3.0, its performance was similar to that observed in the bench‐scale unit (92% COD removal for r = 3.0). However, the nitrogen removal was lower (55% N removal for r = 3.0) due to problems with the hydrodynamics in the aerobic zone. The anaerobic–aerobic fixed‐bed reactor with recirculation of the liquid phase allows for concomitant carbon and nitrogen removal without adding an exogenous source of electron donors and without requiring any additional alkalinity supplementation.  相似文献   

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