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1.
A. I. Kopylov A. F. Sazhin E. A. Zabotkina A. V. Romanenko N. D. Romanova P. R. Makarevich M. P. Wenger 《Oceanology》2016,56(6):789-798
The paper considers the concentrations and functional characteristics of viruses, bacteria, and heterotrophic nanoflagellates determined for the first time in the Laptev Sea in August-September, 2014. The abundance of bacteria, viruses, and heterotrophic nanoflagellates varied from 110.1 × 103 to 828.4 × 103 cells/mL, from 384.2 × 103 to 2932.8 × 103 particles/mL, and from 108 to 651 cells/mL, respectively. The daily bacterioplankton production varied from 4.2 × 103 to 381.7 × 103 cells/mL, with an average of 117.6 × 103 cells/mL. Electron transmission microscopy has for the first time shown that the frequency of visibly infected bacterial cells varied from 0.2 to 2.0% (0.8% on average) of NB. The average virus-induced mortality of bacteria was 6.3% of bacterioplankton production, with variations ranging from 1.4 to 16.9%. Grazing on bacteria by heterotrophic nanoflagellates contributed more to bacteria mortality than virus-induced bacterial lysis. By grazing on bacteria, heterotrophic nanoflagellates consumed large quantities of viruses located on the surface and inside bacterial cells. 相似文献
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胶州湾浮游病毒的分布研究 总被引：3，自引：1，他引：2
运用荧光显微镜技术,对2007年6～8月胶州湾14个站点的浮游病毒丰度进行了检测,分析了病毒在不同月份的水平与垂直分布变化,发现胶州湾浮游病毒的丰度在0.48×107～22.78×107个/mL之间,平均值为(5.72±4.72)×107个/mL,7月份病毒丰度明显高于其他两个月(P0.01)。病毒呈现从湾内至湾口至湾外递减的趋势,病毒垂直分布变化不明显。病毒-细菌比率(VBR)范围为3.90～150.72,平均值42.05±28.55,处于较高水平。利用多元相关性分析发现,病毒丰度与异养细菌丰度、聚球藻蓝细菌丰度和叶绿素a含量相关,其相关系数r分别为0.605(P0.01),0.265(P0.01)和0.604(P0.05),确定系数R2分析表明,异养细菌和叶绿素a对浮游病毒丰度的影响基本相当。病毒丰度与温度、盐度无明显相关性。对VBR的分析表明,调查区域藻类病毒占总浮游病毒的比例较高;通过VBR与异养细菌丰度的负相关性分析,认为胶州湾噬菌体的宿主菌种群较单一。 相似文献
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2012年夏季中国第5次北极科学考察期间,对北冰洋楚科奇海及其北部边缘海浮游细菌丰度和生产力进行了测定,并将其与环境因子进行了相关性分析。结果显示,楚科奇海浮游细菌丰度的变化范围为0.56×108~6.41×108 cells/dm3,平均为2.25×108 cells/dm3;细菌生产力介于0.042~1.92mg/(m3·d)(以碳计)之间,平均为0.54mg/(m3·d)(以碳计),与已有研究结果基本相当。陆架区细菌丰度和生产力要明显高于北部边缘区,但前者的单位细菌生产力则较低。与环境因子的相关性分析显示,细菌丰度与温度和叶绿素a浓度存在显著正相关(p0.01),表明北极变暖导致的海水升温及浮游植物生物量的增加均会促进细菌的生长,从而进一步提高细菌在海洋生态系统和碳循环中的作用。但陆架区的细菌生产力与环境参数均没有显著相关性,表明其影响因素较为复杂;生产力在北部边缘区则仅与叶绿素a存在显著正相关(p0.01),表明浮游植物生长过程产生的溶解有机碳(DOC)是细菌生长最为主要的碳源,碳源的单一可能制约细菌的生产从而导致该海域无冰状态下细菌丰度的增加不如预期,但融冰过程带来的大量DOC将促进细菌活性的增加。 相似文献
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胶州湾夏季异养浮游细菌的时空变化规律及影响因素 总被引：3，自引：0，他引：3
2001年夏季对胶州湾异养浮游细菌在一个潮周期内的变化规律及影响因素进行了研究。结果表明,胶州湾异养浮游细菌数量的垂直分布特征是表层大于底层,表层平均8.99×109个/L,底层平均5.23×109个/L。胶州湾水体异养浮游细菌日变化幅度在表层水体较为明显,底层相对较小,但其变化规律均为最高值在小潮期而最低值出现在大潮期。浮游动物摄食、浮游植物光合作用产生的溶解有机物及水温和日光中的紫外辐射是影响胶州湾异养浮游细菌昼夜变化的主要因素,水交换是影响其日变化的主要因素。 相似文献
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养殖活动对超微型浮游生物分布影响的研究 总被引：2，自引：1，他引：1
利用流式细胞仪对河北省扇贝养殖区微微型浮游植物、异养细菌、浮游病毒4季的丰度分布特征进行了研究,分析了三者与环境因子的相关性,并与渤海、北黄海非养殖区的超微型浮游生物丰度的分布特征进行对比。结果显示:在养殖区海域,聚球藻丰度在9.00×102—7.07×105cell/m L之间,峰值出现在秋季,且与其他季节差异显著(P0.01)。微微型真核藻类丰度在5.80×102—3.23×105cell/m L之间,夏季赤潮暴发期间,丰度达到3.23×105cell/m L,显著高于其他季节(P0.01)。异养细菌丰度在3.10×105—3.79×106cell/m L之间,峰值出现在秋季,夏、秋季丰度显著高于春、冬季(P0.01)。浮游病毒丰度在2.50×105—2.17×106cell/m L之间,峰值出现在秋季,但无显著性季节差异(P0.05)。通过主成分分析发现,聚球藻、微微型真核藻类、异养细菌和浮游病毒的丰度在不同季节受到不同环境因子的影响。在春、冬季,温度是主要影响因素;而在夏、秋季,主要受到营养盐的影响。养殖区与非养殖区超微型浮游生物主成分4季均有显著差异,养殖区异养细菌4季均是超微型浮游生物的主成分,而非养殖区超微型浮游生物的主成分4季均是微微型浮游植物,结果表明养殖活动显著影响了养殖区超微型浮游生物的群落结构和功能。 相似文献
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北部湾北部海域水体异养细菌的时空分布特征研究 总被引：2，自引：1，他引：1
为探讨环境因素对异养细菌丰度的影响，2016年9月至2017年8月通过月度航次调查对北部湾北部海域异养细菌丰度的时空分布特征进行了系统研究。结果表明，调查海区异养细菌丰度介于（2.75~56.86）×105 cell/mL，平均值为（11.01±6.31）×105 cell/mL。各季节细菌丰度从高至低依次为:夏季、春季、冬季、秋季。异养细菌丰度由近岸海域向西南深水区方向逐渐降低，在近岸浅水区垂直分布均匀，在水深大于20 m的海区出现季节性分层现象:表层细菌丰度较高，底层细菌丰度较低。主成分分析显示温度对异养细菌时空分布有重要影响，秋、冬季异养细菌丰度与温度呈显著负相关，在春、夏季呈显著正相关。细菌丰度与盐度呈显著负相关，说明海水盐度变化是细菌时空分布重要影响因素。异养细菌丰度与叶绿素a和溶解氧含量呈显著正相关，表明浮游植物初级生产过程影响了异养细菌的时空分布。在秋、冬和春3季异养细菌丰度与营养盐水平呈显著负相关，二者关系受浮游植物生物量间接影响。异养细菌时空分布差异取决于环境条件的变化，温度、盐度、叶绿素a和溶解氧含量是影响异养细菌丰度分布的主要因素。 相似文献
9.
An-Yi Tsai Gwo-Ching Gong Robert W. Sanders Chien-Fu Chao Kuo-Ping Chiang 《Journal of Oceanography》2010,66(6):873-883
This two-year study investigates the possible factors that determine spatial and temporal dynamics of picoplankton (heterotrophic
bacteria, autotrophic picoplankton—Synechococcus spp., Prochlorococcus, and picoeukaryotes) and nanoflagellate abundance in the subtropical Ilan Bay, Taiwan, where the inner bay is affected by
freshwater run-off from the Lanyang River and the eastern outer bay by the Kuroshio Current. In the inner bay, there was more
rain and freshwater discharge in 2005 than in 2004 during the warm season (>24° C, June–September). The abundance of bacteria,
Synechococcus spp., Prochlorococcus, and picoeukaryotes and the percentage contributions of pigmented nanoflagellate (PNF %) were two- to eight-fold greater
during this period (July in 2005) than for other sampling periods. Relatively low abundance of heterotrophic nanoflagellates
(HNF) in the presence of abundant picoplankton prey suggests that top-down control determined HNF abundance in the Ilan Bay,
Taiwan. 相似文献
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Role of environment and hydrography in determining the picoplankton community structure of Sagami Bay,Japan 总被引：1，自引：0，他引：1
Smita Mitbavkar Toshiro Saino Naho Horimoto Jota Kanda Takashi Ishimaru 《Journal of Oceanography》2009,65(2):195-208
Seasonal variations in the picoplankton community were investigated from June 2002 to March 2004 within the photic zone of
Sagami Bay, Japan. The study area was mostly dominated by coastal waters during the warm period (mixed layer water temperature
≥ 18°C). During the cold period (mixed layer water temperature ≤ 18°C), the water mass was characterized by low temperature
and high saline waters indicative of the North Pacific Subtropical Mode Water (NPSTMW). Occasionally, a third type of water
mass characterized by high temperature and low saline properties was observed, which could be evidence of the intrusion of
warm Kuroshio waters. Synechococcus was the dominant picophytoplankton (5−28 × 1011 cells m−2) followed by Prochlorococcus (1−5 × 1011 cells m−2) and picoeukaryotes during the warm period. Heterotrophic bacteria dominated the picoplankton community throughout the year,
especially in the warm period. During the Kuroshio Current advection, cyanobacterial abundance was high whereas that of picoeukaryotes
and heterotrophic bacteria was low. During the cold period, homogeneously distributed, lower picophytoplankton cell densities
were observed. The dominance of Synechococcus in the warm period reflects the importance of high temperature, low salinity and high Photosynthetically Active Radiation
(PAR) on its distribution. Cyanobacterial and heterotrophic bacterial abundance showed a positive correlation with temperature.
Prochlorococcus and picoeukaryotes showed a positive correlation with nutrients. Picoeukaryotes were the major contributors to the picophytoplankton
carbon biomass. The annual picophytoplankton contribution to the photosynthetic biomass was 32 ± 4%. These observations suggest
that the environmental conditions, combined with the seasonal variability in the source of the water mass, determines the
community structure of picoplankton, which contributes substantially to the phytoplankton biomass and can play a very important
role in the food web dynamics of Sagami Bay. 相似文献
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Kuninao Tada Koji Monaka Masakazu Morishita Toshiya Hashimoto 《Journal of Oceanography》1998,54(4):285-295
Standing stocks and production rates of phytoplankton and abundance of bacteria were investigated at 39 stations in the Seto
Inland Sea, Japan during four crulses in October 1993, January, April and June 1994. Primary productivity was measured by
the13C tracer method. Photosynthetic rate varied from 0.41 to 32.1 μgC/1/h with an average value of 4.67 μgC/l/h. Annual primary
production was estimated to be 218 gC/m2/year. Annual primary production in this study was 1.8 times as high as the values which were previously reported at same
area. The reason for the disagreement between our primary production value and previous values is not thought to be due to
the difference of methods used for measuring primary production or the different Chl.a concentrations but to the method of estimating the primary production in the euphotic zone from thein vitro measurements. The distribution of bacterial cells in surface seawater was examined during the same cruises. Bacterial cell
density ranged from 0.32 to 3.4×106 cells/ml. The density was relatively high in the eutrophic regions of Hiroshima Bay and Osaka Bay In addition, a high density
of bacteria was also observed in an area within Suo Nada where Chl.a was relatively low. The disparity between Chla and bacterial density in Suo Nada suggests that bacterial abundance can be controlled by the availability of substrates other
than phytoplankton exudate. 相似文献
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渤海、黄海沿岸几种经济贝类及其生存环境中的异养细菌 总被引：10，自引：0，他引：10
报道渤海、黄海6个重点沿岸海域(吕四、青岛、北戴河、盘锦、大连、庄河)海水和沉积物及经济贝类样品中的异养细菌总数检测结果.结果表明,整个渤海、黄海沿岸海域经济贝类体内异养细菌总数的变化范围为2.0×103～1.6×106cfu/g湿重,平均值为2.7×105cfu/g湿重,其中异养细菌总数最高的样品是在吕四沿岸3号站采集的四角蛤蜊(1.6×106cfu/g湿重),其次分别在北戴河沿岸2号站采集的杂色蛤(1.2×106cfu/g湿重)和毛蚶(1.1×106cfu/g湿重),而异养细菌总数最低样品是在青岛沿岸采集的镜蛤(2.0×103cfu/g湿重)和在大连沿岸采集的太平洋牡蛎、紫贻贝和皱纹盘鲍(≤5.0×103cfu/g湿重).该海区表层海水中异养细菌总数变化范围为2.5×105～1.0×108cfu/dm3,全海域平均值为1.2×107cfu/dm3.表层沉积物中异养细菌数量变化范围为2.8×103～7.5×105cfu/g干重,全海域平均值为1.3×105cfu/g干重.根据上述结果和欧共体委员会(93/51/EEC)指令要求,对各海区贝类卫生质量进行了初步评价.这在国内尚属首次. 相似文献
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2006年10月在黄海冷水团海域的三个站点开展了微型异养鞭毛虫、异养细菌和蓝细菌的密度和生物量调查,进行了微型异养鞭毛虫的现场摄食实验,通过荧光标记细菌法和消化系数法获得该海区微型异养鞭毛虫对异养细菌和蓝细菌的摄食率,并估算了微型异养鞭毛虫对异养细菌和蓝细菌现存量及生产力的摄食压。结果显示,微型异养鞭毛虫、异养细菌和蓝细菌的密度分别为0.36×103~1.13×103,0.39×106~1.13×106和0.04×104~3.74×104cells/cm3,温跃层以上明显高于底层。微型异养鞭毛虫对异养细菌的摄食率为5.33~14.89个/(HF·h),对蓝细菌的摄食率为0.26×102~23.10×10-2cells/(HF·h),摄食率随深度而下降。微型异养鞭毛虫每天能消耗9.27%~33.08%的异养细菌现存量和8.12%~16.09%的蓝细菌现存量。同时,微型异养鞭毛虫对异养细菌和蓝细菌的日摄食量各占它们生产力的2.66%~13.10%和8.12%~16.09%。研究表明微型异养鞭毛虫的摄食可能不是秋季黄海冷水团海域浮游细菌及其生产力的主归宿。 相似文献
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On the basis of luminescent microscopy using a DAPI dye, the abundance, morphometric characteristics, and biomass of bacteria in the coastal waters off the northeastern part of the Black Sea in 2005 were considered. The material was collected in June, September, and October along the cross section from Golubaya Bay towards the open part of the sea. An analysis of the microorganism development level showed that the bacteria achieved their maximal abundance in September, amounting, in the closest near-shore area, to 2435 × 103 cells/ml, or 276 mg/m3 of wet weight; the lowest values were characteristic of October. During all the survey periods, coccal forms were prevailing among the microorganisms; regardless of the season, their fraction was maximal at the coastal stations. The greatest prevalence of coccal forms was characteristic of October (83%). The average sizes of the bacterial cells in 2005 varied within 0.073–0.257 μm3. The highest values of the bacterial abundance and biomass, almost in all the cases, were found at coastal stations; with the distance from the coast, a decrease in the bacterioplankton abundance was observed. A comparison of methodically similar data on the concentration of bacteria (dying with acridine orange and DAPI) from 1985 till now shows a trend of the annual growth in the bacterial abundance. 相似文献
15.
QU Liang WANG Renjun ZHAO Peng CHEN Ruinan ZHOUWenli TANG Liuqing TANG Xuexi 《海洋学报(英文版)》2014,33(1):135-140
Research of interaction mechanism between Chlorella vulgaris and two bacterial strains(Z-QD08 and Z-QS01)were conducted under laboratory conditions.Growth rates of bacteria and C.vulgaris were tested under co-culture conditions to evaluate the effects of concentrations of C.vulgaris and bacteria on their interactions.To test whether the availability of inorganic nutrients,vitamins and trace metals affects the interactions between C.vulgaris and bacteria,experiments were performed with or without the culture medium filtrate of C.vulgaris or bacteria.The results showed that the growth of C.vulgaris was promoted at low concentrations of bacteria(5×106cells/ml),and expressed a positive correlation with the bacteria density,whereas opposite trend was observed for treatments with high bacteria density(10×106cells/ml and 20×106cells/ml).The growth rate of bacteria decreased with the increasing concentrations of C.vulgaris.The growth of bacteria Z-QD08 was inhibited by C.vulgaris through interference competition,while the mechanism for interaction between bacteria Z-QS01 and C.vulgaris was resource competition.The influence of cell density on the interaction between microalgae and bacteria was also discussed.These experiments confirm some elements of published theory on interactions between heterotrophic bacteria and microalgae and suggest that heterotrophic bacteria play an important role in the development of blooms in natural waters. 相似文献
16.
Flow cytometry investigation of picoplankton across latitudes and along the circum Antarctic Ocean 总被引：2，自引：1，他引：1
Using a flow cytometer (FCM) onboard the R/V Xuelong during the 24th Chinese Antarctic cruise, picoplankton community structure and biomass in the surface water were examined along the latitude and around the Antarctic Ocean. Salinity and temperature were automatically recorded and total Chla was determined. Along the cruise, the abundance of Synechococcus, Prochlorococcus, pico-eukaryotes and heterotrophic bacteria ranged in 0.001-1.855×108 ind./L, 0.000-2.778￡108 ind./L, 0.002-1.060×108 ind./L and 0.132-27.073×108 ind./L, respectively. Major oceanic distribution of Synechococcus and Prochlorococcus appeared between latitudes 30°N and 30°S. Prochlorococcus was mainly influenced by water temperature, water mass combination and freshwater inflow. Meanwhile, Synechococcus distribution was significantly associated with landing freshwater inflow. Pico-eukaryotes and heterotrophic bacteria were distributed all over the oceans, but with a relatively low abundance in the high latitudes of the Antarctic Ocean. Principal Component Analysis showed that at same latitude of Atlantic Ocean and Indian Ocean, picoplankton distribution and constitution were totally different, geographical location and different water masses combination would be main reasons. 相似文献
17.
2011年胶州湾网采浮游植物群落结构及其环境影响因子 总被引：2，自引：1，他引：1
本文于2011年1-12月对胶州湾海域浮游植物进行了周年调查,获得了同步的温盐和营养盐数据,分析了浮游植物丰度、优势种类、多样性指数和季节变化特征及其与环境影响因子的关系。结果表明：共检出浮游植物108种（包括变种和变型）,其中硅藻门34属93种,甲藻门6属14种,金藻门1属1种。浮游植物主要由硅藻和甲藻两大类组成,其中硅藻占绝对优势,全年甲藻/硅藻比均小于0.08。优势种共有35种,夏、冬季优势种交替明显,全年几乎均出现的种类为圆筛藻（Coscinodiscus spp.）,旋链角毛藻（Chaetoceros curvisetus）和中肋骨条藻（Skeletonema costatum）。生态类型主要以广布种、广温近岸种、温带近岸种为主,也出现了少数暖水种和外洋种。浮游植物丰度分别在2月、4月和7月份达到峰值,其中4月全年最高,为591×106cell/m3,2月是次高峰,数量为468×106cell/m3,7月为172×106cell/m3,秋季浮游植物丰度相对较低。本研究结果与胶州湾浮游植物典型双峰型变化不同。浮游植物群落多样性指数平均为2.4,秋、冬季节较高,春、夏季节偏低,水平分布上呈现湾外 > 湾口 > 湾内的趋势,均匀度平均值为0.26,分布趋势与多样性指数基本相同。由此可见,湾外浮游植物群落结构较湾口和湾内更为稳定,温度、硅酸盐、亚硝酸盐、硝酸盐、铵盐和磷酸盐对浮游植物丰度和群落结构存在明显影响。 相似文献
18.
2001—2002年粤东柘林湾浮游动物的生态学研究 总被引：25，自引：3，他引：25
2001年4月-2002年4月,利用生态学方法对粤东柘林湾浮游动物进行的周年调查结果表明,粤东柘林湾浮游动物有桡足类34属60种,枝角类3属3种,及端足类、磷虾、糠虾、多毛类、毛颚类、被囊类、水母和各种浮游幼体虫.浮游动物的群落构成小型化趋势明显,因为体长不足0.6 mm的小型优势种强额拟哲水蚤(Paracalanus crassirostris)、短角长腹剑水蚤(Oithona brevicornis)和鸟喙尖头(氵蚤)(Penilia avirostris)在浮游动物总个体数中所占比例合计高达57.1 %.浮游动物的种类数、总个体数和生物量的平面分布模式大体相似,即湾外大于湾内,外侧大于内侧,东部大于西部.总个体数与生物量的周年变化曲线与水温的变化趋势非常相似,高峰位于高温季节的8-9月,低谷位于冬季2月.调查期间柘林湾浮游动物非常丰富,年均总个体数达15.8×103 ind/m3,生物量达227.8mg*dW/m3.在单一调查年度内,浮游动物丰度与水温、浮游植物细胞数呈显著正相关关系.在不同年份,浮游动物丰度与浮游植物密度则表现为负相关的趋势. 相似文献
19.
The KwaZulu-Natal Bight is a shallow indentation of the eastern seaboard of South Africa, characterised by a narrow (45 km wide) extension of the continental shelf, with a shelf break at about 100 m. It has a complex hydrography: the waters of the bight are derived from the fast-flowing, southward-trending Agulhas Current, which is fed mostly by the tropical and subtropical surface waters of the South-West Indian Ocean subgyre, which are generally oligotrophic in nature, notably depleted in reduced nitrogen and phosphate except at river mouths and during periodic upwelling of deeper nutrient-rich water. Despite this, the bight is believed to be relatively productive, and it is suggested that efficient nutrient recycling by prokaryotes may sustain primary productivity efficiently, even in the absence of new nutrient inputs. Here we have measured bacterial numbers, biomass and heterotrophic productivity during summer and winter in conjunction with phytoplankton standing stock and factors that influence it. Bacterial distribution closely matched phytoplankton distribution in surface waters, and was highest close to the coast. Bacterial standing stocks were similar to those of oligotrophic systems elsewhere (0.5–5.0 × 105 cells ml–1; 1 × 10–8 to 1.25 × 10–7 g C ml–1) and increased in association with the development of phytoplankton blooms offshore and with inputs of allochthonous material by rivers at the coast. Heterotrophic productivity in summer was lowest in the far south and north of the bight (0.5 × 10–10 g C ml–1 h–1) but higher close to the shore, over shallow banks, and in association with increased phytoplankton abundance over the midshelf (1.0–3.5 × 10–9 g C ml–1 h–1). There were marked seasonal differences with lower bacterial standing stocks (5 × 104 to 2 × 105 cells ml–1; 4–5 × 10–9 to 1–2 × 10–8 g C ml–1) and very low bacterial productivity (4 × 10–11 to 1 × 10–10 g C ml–1 h–1) in winter, probably resulting from lowered rates of primary productivity and dissolved organic matter release as well as reduced riverine allochthonous inputs during the winter drought. 相似文献
20.
胶州湾表层沉积物中甲藻孢囊的分布 总被引：3，自引：0，他引：3
为了解胶州湾表层沉积物中甲藻孢囊的分布特征,于2015年7月采集胶州湾12个站位的表层沉积物进行甲藻孢囊分析,共鉴定甲藻孢囊37种(分属5个类群)和2种未定种,包括自养型20种和异养型17种,优势种为膝沟藻。其Shannon-Wiener多样性指数[H′(log2)]介于2.84—3.91,平均值为3.42;均匀度指数介于0.75—0.98,平均值为0.88,总体值较高。胶州湾海域各站位的甲藻孢囊丰度介于96—969cyst/g DW(干重),平均丰度为401cyst/g DW,呈现出临近湾口处丰度较高,而湾内较低的空间分布差异特征。尤其值得注意的是,有两种产虾夷扇贝毒素(Yessotoxin,YTX)甲藻[具刺膝沟藻(Gonyaulax spinifera)和多边舌甲藻(Lingulodinium polyedrum)]的孢囊和3种产麻痹性贝类毒素(PSP)甲藻[塔玛/链状亚历山大藻(Alexandrium catenella/tamarense)、微小/相似亚历山大藻(A.minutum/affine)和链状裸甲藻(Gymnodinium catenatum)]的孢囊在胶州湾海域大多数站位均有分布,尽管其数量不高,但其广泛的分布范围仍需引起我们的重视。 相似文献