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1.
Halophilic archaea comprise the majority of microorganisms found in hypersaline environments. C50 carotenoids accumulated in archaea cells are considered potential biotechnological products and possess a number of biological functions. Ten red colordes were isolated from brine water in a saltem crystaltizer pond of the Hangu Saltworks, China. 16S rRNA gene sequence analysis showed that the colonies belonged to the extremely halophilic archaea genera Halobacterium and Halorubrum. Two representative strains, Halobacterium strain SP-2 and Halorubrum strain SP-4, were selected for further study on the phenotypic characteristics and effects of salinity and pH on accumulation and composition of pigments in their cells. The archaeal strains were isolated and grown in a culture medium prepared by dissolving yeast extract （10 g/L） and acid-hydrolyzed casein （7.5 g/L） into brine water obtained from a I.ocal salt pond. Their optimum salinity and pH for growth were 250 and 7, respectively, although pigment accumulation （OD490/ mL broth） was highest at pH 8. In addition, at 150-300 salinity, increasing salinity resulted in decreasing pigment accumulation. Analysis of the UV-Vis spectrum, TLC and HLPC chromatograms showed that C50 carotenoid bacterioruberin is the major pigment in both strains.  相似文献   

2.
The objective of this study was to provide reliable basis for decision making for national food security and layout and structure adjustment of grain production in the northeastern China. The data of mean daily air temperature of 1961-2009 from 106 meteorological stations in the northeastern China were chosen in this study. Using statistical methods and isoline method, the spatio-temporal changes of various decadal ≥ 10℃ accumulated temperature and the climatic means of ≥ 10℃ accumulated temperature were studied in this paper. The results showed that 1) The geo-graphical distribution of ≥ 10℃ accumulated temperature in the northeastern China could be influenced directly by the latitude, longitude and altitude. If latitude moved one degree northward, the average decrease amplitude of the climatic means was 101.9℃ in the study area. 2) The means of decadal ≥ 10℃ accumulated temperature rose since the 1980s, and their increase amplitudes became larger in the 1990s and the 2010s obviously. Compared with those of the 1980s, ≥ 10℃ accumulated temperature increased by about 100℃ in the mountainous and plain areas in the 1990s; compared with those of the 1990s, ≥ 10℃ accumulated temperature increased by about 200℃ in the Hulun Buir High Plain and the Songnen Plain, and 100℃ in the Sanjiang Plain and the Liaohe Plain in the 2010s. 3) The means of the decadal ≥ 10℃ accumulated temperature for 106 meteorological stations in the northeastern China increased with the rate of 145.57℃/10yr in 1961-2009. 4) The climatic means of ≥ 10℃ accumulated temperature increased from 1961-1990 to 1971-2000 and 1981-2009. Compared with the climatic mean of 1971-2000, that of 1981-2009 had increased by above 50℃ in most of the study area, even up to 156℃. Compared with the climatic mean of 1961-1990, that of 1981-2009 increased by above 100℃ in most parts of the study area, even up to 200℃. 5) The maximum northward shift, eastward and westward extension amplitudes of 3100℃, 3300℃ and 3500℃ isolines were larger among all isoli-nes for the climatic means of the three phases. Compared with the positions of the isolines of 1961-1990, those ampli-tudes of 3100℃ isoline of 1981-2009 were 145 km, 109 km and 64 km, respectively; those of 3300℃ isoline were 154 km, 54 km and 64 km, respectively; and the maximum northward shift of 3500℃ isoline was about 100 km.  相似文献   

3.
The inadequate nutrient content of pellet feeds widely used in recent years in China for grass carp forming led to lipid liver degeneration in the fish. The present studies show that the pathological features of lipid liver disease are anaemia and hepatic ceroidosis. Other clinical features are: the ratio of liver to body weight exceeds 3% and lipid content exceeds 5%. Extreme infiltration of hepaiocytes by lipid results in the following deteriorative effects: swelling of the liver cells, increase of lipid droplets in the cytoplasm and dislocation of the nucleus, loss of cytoplasm staining affinity, and increased activities of GOT and GPT in serum. Lipid liver degeneration of grass carp can be divided into three stages: 1) deposition of liver lipid; 2) lipid infiltration of hepatic parenchyma; 3) atrophy of liver nucleus. The causes of lipid liver degeneration are complicated, but the main cause is assumed to be an imbalance of nutrients in daily feed and the lack of some lipotropic substances.  相似文献   

4.
This paper discusses the spatial and seasonal distribution character of dissolved and paniculate trace metals in the anoxic seawater of Saanich Inlet, B.C., Canada. The study showed that concentration of dissolved and paniculate trace metals in anoxic seawater is closely related to (1) the concentration of H2S and the depth of the O2-H2S interface, (2) the exchange of seawater in Saanich Inlet with outside seawater, (3) biotic action, and (4) the flushing event. The study was based in part on the ratio between trace metals and nutrients. There was a steep change in the concentration of dissolved trace metals at the O2-H2S interface. The concentration of dissolved trace metals in the H2S-controlled zone was dependent on the H2S there. The suspended matter in Saanich Inlet comes from the Hero Strait seawater, phytoplankton production and resuspension of flushed-up sediments. The concentration of particulate trace metals was rather low in Saanich Inlet and tended to increase with depth. The total concentra  相似文献   

5.
The vertical fluxes and molar ratios of carbon, nitrogen and phosphorus of suspended particulate matter in the Yellow Sea were studied based on the analysis of suspended particulate matter,sediments and sinking particles obtained by use of moored sediment traps. The POC ： PON ratios indicate that most of the particulate organic matter in the Yellow Sea water column comes from marine life rather than the continent. The vertical fluxes of SPM, POC, PON and POP in the Yellow Sea are much higher than those in other seas over the world, and present a typical pattern in shallow epicontinental seas. The estimated residence time of the bioactive elements showed that the speed of the biogeochemical process of materials in the Yellow Sea is much shorter than that in the open ocean as there was high primary productivity in this region.  相似文献   

6.
Gracilaria salicornia (C. Agardh) Dawson is a distinctive species based upon Sphaerococcus salicornia C. Agardh (1820), which was transfered to Corallopsis by Greville in 1830. Dawson (1954) made the new combination on the basis of specimens collected from Manila Bay, the Philippines. This species has now been found to be widely distributed throughout the Philippine Islands (Trono, 1983), as well as in Koror, Palau group of the Caroline Islands (Trono,1969), Solomon Islands (Womersley and Bailey, 1970), China (Chang and Xia, 1976; Yang and Chiang, 1982), and southern Japan (Yamamoto, 1978). The constricted branches that form obcuneate articulations provide a ready and, when judged in isolation, clear feature by which the taxon can be recognized.  相似文献   

7.
Changes in permanent sample plots in the lowland,submontane and montane forests on Mount Cameroon(4,095 m above sea level),an active volcano,are described for 15 years from 1989 to 2004.Throughout the study period,the stocking level of trees with a diameter at breast height(DBH) ≥ 10 cm in the three forests were lower than in pan-tropical stands suggesting a significant impact of volcanic and human-related activities on the vegetation communities on the mountain.Annual mortality rates in the submontane and montane forests were consistent with those reported for comparable altitudinal ranges in the Blue Mountains of Jamaica.The annual mortality rate was higher in the lowland forest than other lowland sites included.Divergence between recruitment and mortality rates was large suggesting that the three vegetation communities have not reached their climax.The seven-year difference in half-life of large trees(with a DBH ≥ 50 cm) in the submontane and montane forests suggests an altitudinal effect on turnover of larger trees that in turn contributes to the frequent small stature of high altitude forests.There was little evidence of an altitudinal effect on species turnover and growth rate.This finding supports generalizations about the zero effect of growth on the stature of high altitude trees.Understanding forest dynamics is crucially important in the management of tropical montane environmentsand in this instance particularly so given the recent creation of the Mount Cameroon National Park.  相似文献   

8.
This paper reports the concentrations of 137 Cs, hexachlorocyclohexane(HCH), dichlorodiphenyltrichloroethanes(DDT) and its main degradation products, δ13C, and organic carbon in pond sediments(0-210 cm, sectioned by 2-20 cm interval) and surface soils(the 0-3 cm horizon) collected in 2010 from Chenjia catchment, which is located in Yanting county in the hilly central Sichuan of China. α-, δ-, and γ-HCH, DDT, and DDD were not detected throughout the sediment profile. Trace concentrations of δ-HCH(0.89-29.31 ng g-1) and p,p′-DDE(1.85-6.02 ng g-1) were detected only in top 40 cm sediment. The 137 Cs fallout peak in 1963(corresponding to the 55-60 cm depth), the sedimentary signature left by the last year of HCH use in 1989(an additional indicator at 20-25 cm), and the obvious original channel bed prior to the construction of the pond in 1956 were used as temporal markers to estimate changes in average sedimentation rate between different periods due to changes in land use. Continuous, marked decrease in average sedimentation rate(i.e., 3.79, 1.35 and 1.07 cm year-1 in 1956-1963, 1963-1989, and 1989-2010, respectively)over time was observed, probably due to the reforestation, abandoning of steep sloping farmland for afforestation and natural re-vegetation(implementation of the Grain for Green Program), and the conversion of part of gently sloping farmland terraces to orchard land since the 1980 s, especially since the 1990 s. This was corroborated by the observed decrease(more negative) in δ13C of sediment towards the surface, which indicates increased relative contribution of eroded soil particles coming from slopes with increased tree cover in sediment source area. Combined use of 137 Cs, δ-HCH, and δ13C record in sediments has been demonstrated to be a powerful approach to reconstruction of response in sedimentation rate to historical land use changes.  相似文献   

9.
1Introduction Activities of extracellular enzymes can serve as theindicators of organic matter content.Enzyme assayinghas been used in the analyses of dissolved organic ma-terials in aquatic environments(Chrost,1991),massloss of plant litter in terrestrial systems(Sinsabaughet al.,1992;Sinsabaugh et al.,1994),processing ofpaniculate and dissolved organic carbon(POC,DOC)in streams(Sinsabaugh and Findlay,1995;Findlayet al.,1998),and processing of particulate organicmatter(POM)in wetlands(J…  相似文献   

10.
Wind plays an important role in hydrodynamic processes such as the expansion of Changjiang （Yangtze） River Diluted Water （CDW）, and shelf circulation in the Changjiang estuary. Thus, it is essential to include wind in the numerical simulation of these phenomena. Synthetic aperture radar （SAR） with high resolution and wide spatial coverage is valuable for measuring spatially inhomogeneous ocean surface wind fields. We have collected 87 ERS-2 SAR images with wind-induced streaks that cover the Cbangjiang coastal area, to verify and improve the validity of wind direction retrieval using the 2D fast Fourier transform method. We then used these wind directions as inputs to derive SAR wind speeds using the C-band model. To demonstrate the applicability of the algorithms, we validated the SAR-retrieved wind fields using QuikSCAT measurements and the atmospheric Weather Research Forecasting model. In general, we found good agreement between the datasets, indicating the reliability and applicability of SAR- retrieved algorithms under different atmospheric conditions. We investigated the main error sources of this process, and conducted sensitivity analyses to estimate the wind speed errors caused by the effect of speckle, uncertainties in wind direction, and inaccuracies in the normalized radar cross section. Finally, we used the SAR-retrieved wind fields to simulate the salinity distribution off the Changjiang estuary. The findings of this study will be valuable for wind resource assessment and the development of future numerical ocean models based on SAR images.  相似文献   

11.
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12.
Eleutheronema rhadinum is a potential commercial fisheries species and is subject to intense exploitation in China. Knowledge on the population structure of E. rhadinum in Chinese coastal waters, which is important for sustainable exploitation and proper resource management, is lacking. In the present study, the genetic diversity and population structure of E. rhadinum were evaluated using a 564-base pair fragment of the mitochondrial cytochrome c oxidase subunit I （COl） gene. A total of 76 specimens were collected from three localities around the East （Qidong and Zhoushan） and South China Seas （Zhuhai）. Among these individuals, nine polymorphic sites were detected and 1 l distinct haplotypes were defined. High levels ofhaplotype diversity （h=0.759i0.035） and low levels of nucleotide diversity （re=0.001 98i0.003 26） were observed in these populations, Hierarchical analysis of molecular variance （AMOVA） indicated that 96.72% of the genetic variation occurred within the populations, whereas 3.28% occurred among populations. No significant genealogical branches or clusters were recognized on the neighbor-joining tree. Intra-group variation among populations was significant （~0~t=0.032 85, P〈0.01）. These results suggest that E. rhadinum populations in the East and South China Seas have developed divergent genetic structures. Tests of neutral evolution and mismatch distribution suggest that E. rhadinum may have experienced a population expansion. The present study provides basic information for the conservation and sustainable exploitation of this species.  相似文献   

13.
Viable cell count was used to determine whether Metschnikowia sp.C14 can colonize the intestine of juvenile sea cucumber Apostichopus japonicus.Sea cucumber individuals were divided into two groups,which were fed the control diet for 38 days or the C14-supplemented diet at 105 cells g−1 diet for 28 days,then the control diet from day 29 to day 38.The number of C14 cells in the intestine of sea cucumber fed the C14-supplemented diet significantly increased from day 7 to day 28,and decreased from day 29 to day 38.Sea cucumber fed with the diet containing C14 showed a significant increase in trypsin activity and lipase activity from day 21 to day 33 compared with the control.Feeding C14 significantly improved the phagocytic activity and respiratory burst in coelomocytes from day 21 to day 35 and from day 14 to day 38,respectively.In addition,there was an obvious enhancement in lysozyme activity(from day 21 to day 38 or day 33),phenoloxidase activity(from day 21 to day 28)and total nitric oxide synthase activity(from day 14 to day 38)in coelomic fluid supernatant and/or coelomocyte cell lysate supernatant compared with the control.There were significant positive correlations between the number of C14 cells colonizing the intestine and trypsin activity of the intestine,lysozyme activity of the coelomic fluid supernatant and coelomocyte lysate supernatant from sea cucumber.These data suggested that the number of C14 cells should be maintained at 105 cfu(colony-forming units)g−1 intestine material for the maximum benefit.  相似文献   

14.
The turbidity criterion for the product water of a WTP according to the State Project ‘863＇ on the safeguard technology of drinking water in the southern areas of China is 0.1 NTU. The turbidity removal in the activated carbon filter was analyzed in a pilot-scale test and an innovative technology to improve the turbidity removal in a biologically activated carbon （BAC） filter was put forward in order to meet the criterion. Experimental results showed that the enhanced filtration by adding polymerized aluminium chloride （PAC） into the BAC filter was quite effective in turbidity control. The effluent turbidity was kept at a stable level （mean） of 0.033 NTU with a high removal of about 80% for influent turbidity of 0.110- 0240 NTU with an addition of PAC at 0.05 mg L^-1, meeting the requirement for filtrate turbidity equal to or less than 0.1NTUC totally. In addition, the larger the PAC dosage was, the lower the effluent turbidity was. However, further improvement of turbidity removal was not obvious for PAC dosages beyond 0.10 mg L^- 1, and an optimal PAC dosage in the range of 0.05 - 0.10 mg L^- 1 was proposed.  相似文献   

15.
The size-fractionated phytoplankton biomass, and the spatial and temporal variations in abundance of Synechococcus (SYN) and picoeukaryotes (PEUK) were measured in the Taiwan Strait during three cruises (August 1997, February-March 1998, and August 1998). The results show that picophytoplankton and nanophytoplankton dominate the phytoplankton biomass, in average of 38% and 40%, respectively. SYN and PEUK varied over time in abundance and carbon biomass, greater in summer than in winter, in range of (7.70–20...  相似文献   

16.
Net primary productivity(NPP) and evapotranspiration(ET) are two key variables in the carbon and water cycles of terrestrial ecosystems.In this study,to test a newly developed NPP algorithm designed for HJ-1 A/B data and to evaluate the usage of HJ-1 A/B data in the quantitative assessment of environments,NPP and ET in Jinggangshan city,Jiangxi province,are calculated using HJ-1 A/B data.The results illustrate the following:(1) The NPP and ET in Jinggangshan city in 2010 both show obvious seasonal variation,with the highest values in summer and the lowest values in winter,and relatively higher values were observed in autumn than in spring.(2) The spatial pattern indicates that the annual NPP is high in the southern area in Jinggangshan city and low in the northern area.Additionally,high NPP is distributed in forests located in areas with high elevation,and low NPP is found in croplands at low elevations.ET has no significant north-south difference,with high values in the southeast and northwest and low values in the southwest,and high ET is distributed in forests at low elevations in contrast to low ET in forests in high-elevation areas and in cropland and shrub grassland in low-elevation areas.(3) Compared to the MODIS product,the range of HJ-1 NPP is larger,and the spatial pattern is more coincident with the topography.The range of HJ-1 ET is smaller than that of the MODIS product,and ET is underestimated to some extent but can reflect the effect of topography.This study suggests that the algorithm can be used to estimate NPP and ET in a subtropical monsoon climate if remotely sensed images with high spatial resolution are available.  相似文献   

17.
From 28 March to 17 April, 2018, different forms of mercury(Hg) in the Yellow Sea and Bohai Sea were measured to study the influencing factors on the distribution and transformation of Hg in spring using a shared cruise. The mean concentration of dissolved gaseous mercury(DGM) in the surface water of the Yellow and Bohai Seas was(44.3 ± 43.9) pg/L, which was close to that in mid-latitude oceans and deep seas. The ratio of DGM to THg(total mercury) was lower than in the oceans and in the Yellow and Bohai Seas in summer or fall. DGM concentrations in surface water were highest in the central part of the South Yellow Sea and were higher than those in the Bohai Sea, and their spatial distributions were consistent with RHg(reactive mercury). DGM and RHg correlated positively with water temperature in surface seawater(r = 0.506, P < 0.01;r = 0.278, P < 0.05). The concentrations of both DGM and RHg in surface water were controlled by solar radiation and water temperature. Foggy weather did not benefit the production of DGM and RHg. DGM in the bottom seawater was mainly affected by Dissolved Oxygen and water temperature(r =-0.366, P < 0.01;r = 0.331, P < 0.01), produced mainly by anaerobic reactions of the bottom seawater and sediment microorganisms. The bottom DGM concentrations in the Yellow and Bohai Seas were the highest, and DGM produced in bottom seawater and sediment plays a more important role than the surface water in spring. The concentrations of DGM and RHg in the surface and bottom water in the South Yellow Sea were all higher than those in the middle layer. Vertical variations in the North Yellow Sea and the Bohai Sea were small. The production and distribution of DGM and RHg were influenced by differences of latitude and by the Yellow Sea warm current in spring.  相似文献   

18.
Stable isotopes are increasingly used to investigate seasonal migrations of aquatic organisms. This study employed stable isotopes(δ~(13) and δ15N) for Coilia nasus from the lower Yangtze River and the adjacent East China Sea to distinguish different ecotypic groups, ascertain trophic nutrition positions, and reflect environmental influences on C. nasus. δ~(13)C signatures of C. nasus sampled from Zhoushan(ZS), Chongming(CM), and Jingjiang(JJ) waters were significantly higher than those from the Poyang Lake(PYL)(P 0.05). By contrast, δ~(15)Nsignatures of C. nasus in ZS, CM, and JJ groups were significantly lower than those in PYL group(P 0.05). Basing on δ~(13) and δ~(15)Nsignatures, we could distinguish anadromous(ZS, CM, and JJ) and non-anadromous(PYL) groups. The trophic level(TL) of anadromous C. nasus ranged from 2.90 to 3.04, whereas that of non-anadromous C. nasus was 4.38. C. nasus occupied the middle and top nutrition positions in the marine and Poyang Lake food webs, respectively. C. nasus in Poyang Lake were significantly more enriched in δ~(15)N but depleted in δ~(13), suggesting that anthropogenic nutrient inputs and terrigenous organic carbon are important to the Poyang Lake food web. This study is the first to apply δ~(15)Nand δ~(13) to population assignment studies of C. nasus in the Yangtze River and its affiliated waters. Analysis of stable isotopes(δ~(15)Nand δ~(13)) is shown to be a useful tool for discriminating anadromous and non-anadromous C. nasus.  相似文献   

19.
This paper deals with the bioconcentration of 14C-simetryne from water by aquatic test organisms : green algae-Monoraphidium minutum, rotifers-Brachionus rubens, daphnids-Daphnia magna, and fish-Brachydanio rerio . The chemical was bioconcentrated rapidly in all test species during the first 48 hours of experiment. The BCF values (bioconcentration factor) from all uptake studies show that simetryne has higher accumulation in algae than in rotifers, daphnids and zebra fish.The logarithm of the n-octanol/water partition coefficient of simetryne measured as 2.06+0.05 was correlated with the BCFs in the organisms as based on the lipid contents.14C-simetryne uptake via the food-chain amounted to only 22% to 42% of the bioconcentration from water. Clearance of 14 C- derived residues from fish was rapid with a half-life of 2.1 days.  相似文献   

20.
Four strains of Cylindrotheca (B156, B164, B196, and B200) were harvested at three phases in the growth curve and their total lipid and fatty acid composition were determined. Total lipid reached highest values in the late stationary phase in B156 (24.0%), B164 (26.6%) and B200 (17.3%), in the exponential phase in B196 (15.5%). Saturated fatty acids increased with the devel-opment of the culture in B156 and B196, and reached their highest values in the late stationary phase in B156 (41.7%) and B196 (45.1%), in the early stationary phase in B164 (45.4%) and B200 (37.6%). Monounsaturated fatty acids increased with the development of the culture in B196 and B200, and reached their highest values in the late stationary phase in B196 (32.4%) and B200 (32.8%), in the early stationary phase in B164 (31.0%) and in the exponential phase in B156 (29.3%). Polyunsaturat-ed fatty acids decreased in the later phases of the culture in B164, B196 and B200, and peaked in theexponential phase in B164 (29.5%), B196 (42.9%) and B200 (37.3%), and in the early stationary phase in B156 (32.0%).  相似文献   

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