钝顶螺旋藻部分原生质体及单细胞的制备与培养   总被引：21，自引：0，他引：21  

秦松  王希华  童顺  曾呈奎 《海洋与湖沼》1995,26(1):109-112

于1992年1月-1994年4月，进行超声波处理方法制备钝顶螺旋藻部分原生质体以作为基因工程受体，以及制备单细胞用于固体平板克隆化培养的研究。研究结果表明：超声波以20kHz频率、15W功率作用30s，可使藻丝体断裂成15．0±1．6个细胞长度；延长作用时间，至2－6个细胞长度时，细胞壁结构遭到破坏，形成部分原生质体；继续作用，可形成少量原生质体和大量单细胞。断裂藻丝体、部分原生质体、单细胞以及原生质体均可涂布于固体培养基上再生或生长。以一定密度涂布单细胞与原生质体，能够形成彼此分开的单个克隆，可用于筛选及遗传分析。本文提供了一种节省溶菌酶的制备螺旋藻透性体的方法，超声波作用利于外源基因的导入，而涂布培养利于进一步的筛选和形成克隆。  相似文献   

龙感湖地区近3000年来的植被及其气候定量重建   总被引：18，自引：1，他引：18  

童国榜  吴瑞金 《海洋地质与第四纪地质》1997,17(2):53-61

本文依据龙感湖钻孔的孢粉及硅藻资料，探讨了该地区近３０００年来的植被发展及气候变化，通过数值分析方法，重建了年均温及年降水的时间序列，发现气候波动存在的两个阶段，（依１．５ｋａＢ．Ｐ．前后划分）四个旋回（０．８ｋａ的准周期）两个突变事件（３ｋａＢ．Ｐ）前后的冷暖急剧变化及１ｋａＢ．Ｐ的降温事件）。这些变化与敦德冰心及固城湖孢粉反映的气候波动有较好的一致性，具有全球变化意义。  相似文献   

云贵高原晚新生代孢粉植物群与环境变迁   总被引：8，自引：5，他引：8  

童国榜  羊向东 《海洋地质与第四纪地质》1994,14(3):91-104

本文依据２０多个剖面（钻孔）近７００个样品的孢粉资料及古地磁极性年表建立了云贵高原４Ｍａ以来的孢粉时间序列，并划分了四个区域孢粉组合带，１７个孢粉组合亚带。依据孢粉混杂堆积的出现，认为云贵高原的上升始于２．８ＭａＢ．Ｐ．，加剧于０．８ＭａＢ．Ｐ．前后。前期地形相对高度＜１０００ｍ，后期相对高度达１５００ｍ；地貌经历了开阔盆地－低洼盆地－山地湖盆－高山湖盆的演化。气候经历了古季风、现代季风的发展过程  相似文献   

气溶胶光学厚度的时空变化   总被引：2，自引：0，他引：2  

高飞  李铜基 《海洋技术学报》2003,22(3):15-19

在大气中气溶胶微粒是一种重要的大气微量成分。气溶胶光学厚度也是大气校正所需的重要大气参数，同时也是海洋水色卫星主要的数据产品。由于气溶胶光学厚度的时空变化较大，所以如何准确获取大气校正和卫星数据产品真实性检验所需的气溶胶光学厚度则是至关重要的。在简述气溶胶光学性质的基础上，并结合2002年6月HY—1南海实验数据来阐述现场气溶胶光学厚度的准确获取。  相似文献   

Investigation of the hydrate reservoir production under different depressurization modes     

Zhizeng Xia  Xuewu Wang  Xiaohua Zhang 《Marine Georesources & Geotechnology》2020,38(8):1002-1012

Abstract

Large reserves of natural gas hydrates exist, and the depressurization method has the greatest potential for gas hydrate reservoir recovery. Currently, the most commonly adopted depressurization simulation method is a constant bottom-hole pressure production scheme. This study proposes a new depressurization mode with decreasing bottom-hole pressure. The production characteristic was numerically investigated using this method. The results show the following: (1) As the depressurization exponent (n) decreases, the development effect improves, and production indexes including cumulative gas production/dissociation and gas-water ratio increase. However, the reservoir energy consumption is higher and the hydrate reformation is more severe. (2) Compared to the proposed depressurization mode, the hydrate production index of the constant bottom-hole pressure production (n?=?0) is better. However, the hydrate reservoir energy consumption is higher and the hydrate reformation is more severe using constant bottom-hole pressure production. (3) To achieve a balance between production and reservoir energy consumption during depressurization production, the bottom-hole pressure should be controlled by selecting a suitable depressurization exponent between n_min and n_max, which can be determined through numerical simulations.  相似文献   

五种植物提取液的抗鱼病菌和抗鱼病毒效应(英文)   总被引：1，自引：0，他引：1  

童裳亮  F.M.Hetrick  B.S.Roberson  A.Baya 《中国海洋大学学报(自然科学版)》1990,(2)

本文旨在探索用廉价的野生植物防治鱼病。所用的植物有牛繁缕(Stellaria aqu-atica),双花凤仙(Impatiens biflora),夜来香(Oenothera biennis),艾草(Artem-isia vulgaris)和忍冬(Lonicera japonica)。用作试验的鱼病菌共13种(18个菌株),分属于气单孢菌属、假单孢菌属、爱德华氏菌属、耶尔森氏菌属、弧菌属。所用的鱼病毒为传染性胰脏坏死病毒IPNV,传染性造血组织坏死病毒IHNV。上述病原微生物均获自美国马里兰大学微生物系。实验结果表明,五种植物提取液均有不同程度的抗鱼病菌作用,其中以牛繁缕的抗菌谱最广,作用力最强。忍冬、艾草、牛繁缕有抗鱼病毒作用。其中以忍冬的效果最好,它能抑制IPNV和IHNV。而艾草和牛繁缕只能抑制IHNV。  相似文献   

Design of Mooring System for Oil Storage Vessels     

LI  Wen-long 《中国海洋工程》2003,17(4):541-550

The floating oil storage system has been proposed as a new facility for Strategic Petroleum Reserve (SPR) in China. Mooring is one of the key technologies to ensure the safety, reliability, and performance of the oil storage system. This paper describes the concept, analysis, design and reliability of the mooring system. For mooring system design of these oil vessels, analysis is essential of the behavior of the vessel in connection with mooring facilities of nonlinear resilience. A nonlinear mathematical model for analyzing a moored vessel is established and solved. Some results of numerical simulations are presented. Assessment of the safety regarding the mooring system in terms of failure probability is carried out. Another simulation model for calculating the failure probability of the mooring system is proposed. The design parameters that have an influence on the characteristics of the failure probability have been identified. The simulation results show that the mooring system has an annual reliab  相似文献   

Regression Formulations for the Stress at Hot Spot of Multiplanar Tubular DT Joints     

Chen Tieyun Zhang Huiyuan Professor  Shanghai Jiao Tong University  Shanghai Doctor of Engineering  Shenzhen Shipbuilding Trading Co. LTD. 《中国海洋工程》1993,(1)

- Stress concentration analysis of multiplanar tubular DT joints plays an important role in the fatigue design of offshore platforms. A semi-analytic method for stress analysis under the condition of any loads is briefly introduced in the paper. Nineteen multiplanar tubular DT joints with one of two braces of the same dimension subjected to axial loads and out- of- plane bending moments are computed for parametric stress analysis by using the present method. The influence of geometrical parameters on the stresses of multiplanar tubular DT joints is discussed and compared with corresponding uniplanar T joints. The regression formulae for the stress at hot spot of multiplanar DT joints are found by modification of SCF of corresponding uniplanar T joints. The parametric formulae for the maximum stress by superposition. Finally, the influences of stiffening effect and load-interaction effect on the maximum stress of DT joints are discussed.  相似文献   

基于DXF的OpenFlight数据创建方法研究     

刘立嘉  盛业华  周侗  黄云  陈旻 《测绘科学》2008,33(4)

针对虚拟城市仿真系统应用中OpenFlight模型缺乏、建模方式单一,难以构建复杂模型的情况,本文提出基于CAD文件来构建Opeflght模型的方法,在详细分析OpenFlight和DXF的数据组织结构的基础上,设计了基于DXF的OpenFlight数据创建方案并编程实现。通过实例测试证明该方案能快速、完整地进行数据转换,为虚拟城市系统的三维仿真模型构建提供了一条行之有效的途径。  相似文献   

An integrated assessment of climate change impacts on China's water resources          下载免费PDF全文

REN Guo-yu  JIANG Tong  LI Wei-jing  ZHAI Pan-mao  LUO Yong  MA Zhu-guo 《水科学进展》2008,19(6)

  相似文献