With rapid urbanization process, the pace of urban sprawl has been quickened and most metropolises have experienced expansions in leaps and bounds in suburbanization, which has greatly changed the urban and rural spatial structure of the metropolis fringe area. In this paper, Shuangliu County is taken as a case, since it is a typical fringe area of Chengdu Metropolis. Based on Landsat TM images of Chengdu City and Shuangliu County obtained in 1985, 2000 and 2004, characteristics of urban and rural spatial structure in Shuangliu County were analyzed, such as core-periphery spatial structure and some obvious integration trends. Then the influencing factors for the formation of urban and rural spatial structure were identified, including some natural, social, economic and political factors. According to the discussion of this paper, Shuangliu County is undergoing a key transition and reform period. Its formation of urban and rural spatial structure was affected not only by such common factors as the location, natural background, industrial transition, migration, etc., but also by some unique factors such as city planning and policy guidance, which played an important role in accelerating its uneven spatial structure. The understanding of the role of the influencing factors would be useful for carrying out spatial management and development planning of metropolis fringe area. 相似文献
Outbreaks of large-scale green tides formed by Ulva prolifera in the Yellow Sea(YS)cause heavy ecological damages and huge economic losses.However,the ecological consequences of green tides remain poorly understood due to the lack of knowledge on the settlement region of massive green algae floating in the YS.In this study,we established a method to trace the settlement region of floating green algae,using 28-isofucosterol preserved in sediment as the specific biomarker for green algae.Sterols including 28-isofucosterol in surface sediment samples collected during an investigation in the YS and the Bohai Sea(BS)in August 2015 were analyzed using gas chromatography coupled with a mass spectrometer(GC-MS).Based on the content of 28-isofucosterol in sediment samples,the potential settlement region of loating green algae was identified in the sea area southeast to the Shandong Peninsula around the sampling site H06(122.66°E,36.00°N).This paper presents a first result on the settlement region of floating green algae in the YS for providing a solid basis to elucidate the ecological consequences of green tides in the area. 相似文献
Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( Ho ) and expected heterozygosity ( He ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.
