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1 .IntroductionRecentlygreatinteresthasbeenshowninthedevelopmentofverylargefloatingstructuressuchasMegaFloatofJapan (Isobe ,1 999)andMOBofUSA (Remmers ,1 999) .Owingtotheirextremelargesizeandgreatflexibility ,thecouplingbetweenthestructuraldeformationandfluidmotionissignifi cant.Thisisatypicalproblemofhydroelasticity .Efficientandaccurateestimationofthehydroelasticresponseofverylargefloatingstructuresinwavesisveryimportantfordesign .Manymethodshavebeenproposedinliteratureforthepredictiono… 相似文献
54.
钝顶螺旋藻部分原生质体及单细胞的制备与培养 总被引:21,自引:0,他引:21
于1992年1月-1994年4月,进行超声波处理方法制备钝顶螺旋藻部分原生质体以作为基因工程受体,以及制备单细胞用于固体平板克隆化培养的研究。研究结果表明:超声波以20kHz频率、15W功率作用30s,可使藻丝体断裂成15.0±1.6个细胞长度;延长作用时间,至2-6个细胞长度时,细胞壁结构遭到破坏,形成部分原生质体;继续作用,可形成少量原生质体和大量单细胞。断裂藻丝体、部分原生质体、单细胞以及原生质体均可涂布于固体培养基上再生或生长。以一定密度涂布单细胞与原生质体,能够形成彼此分开的单个克隆,可用于筛选及遗传分析。本文提供了一种节省溶菌酶的制备螺旋藻透性体的方法,超声波作用利于外源基因的导入,而涂布培养利于进一步的筛选和形成克隆。 相似文献
55.
As a fundamental study to evaluate the contribution of the Kuroshio to primary production in the East China Sea (ECS), we
investigated the seasonal pattern of the intrusion from the Kuroshio onto the continental shelf of the ECS and the behavior
of the intruded Kuroshio water, using the RIAM Ocean Model (RIAMOM). The total intruded volume transport across the 200m isobath
line was evaluated as 2.74 Sv in winter and 2.47 Sv in summer, while the intruded transport below 80m was estimated to be
1.32 Sv in winter and 1.64 Sv in summer. Passive tracer experiments revealed that the main intrusion from the Kuroshio to
the shelf area of the ECS, shallower than 80m, takes place through the lower layer northeast of Taiwan in summer, with a volume
transport of 0.19 Sv. Comparative studies show several components affecting the intrusion of the Kuroshio across the 200 m
isobath line. The Kuroshio water intruded less onto the shelf compared with a case without consideration of tide-induced bottom
friction, especially northeast of Taiwan. The variations of the transport from the Taiwan Strait and the east of Taiwan have
considerable effects on the intrusion of the Kuroshio onto the shelf. 相似文献
56.
龙感湖地区近3000年来的植被及其气候定量重建 总被引:18,自引:1,他引:18
本文依据龙感湖钻孔的孢粉及硅藻资料,探讨了该地区近3000年来的植被发展及气候变化,通过数值分析方法,重建了年均温及年降水的时间序列,发现气候波动存在的两个阶段,(依1.5kaB.P.前后划分)四个旋回(0.8ka的准周期)两个突变事件(3kaB.P)前后的冷暖急剧变化及1kaB.P的降温事件)。这些变化与敦德冰心及固城湖孢粉反映的气候波动有较好的一致性,具有全球变化意义。 相似文献
57.
云贵高原晚新生代孢粉植物群与环境变迁 总被引:8,自引:5,他引:8
本文依据20多个剖面(钻孔)近700个样品的孢粉资料及古地磁极性年表建立了云贵高原4Ma以来的孢粉时间序列,并划分了四个区域孢粉组合带,17个孢粉组合亚带。依据孢粉混杂堆积的出现,认为云贵高原的上升始于2.8MaB.P.,加剧于0.8MaB.P.前后。前期地形相对高度<1000m,后期相对高度达1500m;地貌经历了开阔盆地-低洼盆地-山地湖盆-高山湖盆的演化。气候经历了古季风、现代季风的发展过程 相似文献
58.
G. Randy Cutter Jr Robert J. Diaz John Lee 《Deep Sea Research Part II: Topical Studies in Oceanography》1994,41(4-6)
The recent benthic meiofaunal foraminiferal assemblage from the continental slope (590-2 003 m) off Cape Hatteras, North Carolina exhibits high species richness and evenness, moderate diversity values, and lacks numerically dominant species. The preserved planktic assemblage has relatively low species richness, high evenness, low diversity, and a few numerically dominant species. Approximately 9% of the benthic species are those that typically live within continental shelf depth ranges. The benthic assemblage abundances and diversities do not follow depth patterns or geophysical characteristics. No biogeographic boundary can be described within the study area for meiofaunal foraminifera. Oxygen limitation does not appear to be a factor affecting the benthos of the North Carolina continental slope based upon the community structure of the benthic foraminifera, if total assemblage is assumed to reflect the recently living community. The high carbonate content of sediments in the area may be explained by foraminiferal tests. Within the study area, the foraminiferal assemblages are uniform, and probably reflect relative consistency of primary environmental variables as well as dynamic downslope transport and high influx of material from the water column in the vicinity where the Gulf Stream and the Western Boundary Undercurrent cross. 相似文献
59.
This study presents the results of the decoupling of a free-roll decay test by wavelet transforms. A free-roll decay test was performed to determine the coefficients of damping terms in equations of motion. No motion of the model was restricted during the experiment. A slight yaw motion was found while the model was in the free-roll decay motion. It was necessary to extract a pure roll motion from the experimental data. Wavelet transforms were applied to the signals to extract the pure roll motion. The results were compared to those found through the Fourier transform. Discrete wavelet transforms were able to efficiently decouple the test signals, while the continuous wavelet transform and the Fourier transform could not. 相似文献
60.
Todorov JR Elskus AA Schlenk D Ferguson PL Brownawell BJ McElroy AE 《Marine environmental research》2002,54(3-5):691-695
To determine the estrogenicity of effluents from sewage treatment plants (STPs) to larval fish, 2-day-old sunshine bass were exposed to effluents from three STPs serving New York City (NYC), varying in size and treatment level. Estrogenic response was evaluated by measuring vitellogenin (VTG) and estrogen receptor (ER) expression in cytosolic fractions of whole body homogenates. Concentrations of the presumptive endocrine disruptors in the effluents were also measured. VTG and ER levels in sewage-exposed fish were 3-5 times that observed in controls. Combined concentrations of estradiol and estrone ranged from 5 to 13 ng/l and nonylphenol-ethoxylate metabolites (NPEOs: 4-nonylphenol, and 1-, 2-, and 3-nonylphenol-ethoxylates) ranged from 180 to 470 microg/l in chlorinated effluent. Results indicate that both ER and VTG can be used as biomarkers for endocrine disruption in larval fish, and that 4-day exposure to sewage effluent is sufficient to elicit significant expression of these markers in sunshine bass larvae. The extremely higher concentrations of NPEOs found in effluent relative to hormones (approximately 40,000-fold) indicates that surfactant metabolites may be contributing significantly to the estrogenic effects observed. 相似文献