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71.
Presented here is a compact explicit difference scheme of high accuracy for solving the extended Boussinesq equations.For time discretization,a three-stage explicit Runge-Kutta method with TVD property is used at predicting stage,a cubic spline function is adopted at correcting stage,which made the time discretization accuracy up to fourth order;For spatial discretization,a three-point explicit compact difference scheme with arbitrary order accuracy is employed.The extended Boussinesq equations derived by Beji and Nadaoka are solved by the proposed scheme.The numerical results agree well with the experimental data.At the same time,the comparisons of the two numerical results between the present scheme and low accuracy difference method are made,which further show the necessity of using high accuracy scheme to solve the extended Boussinesq equations.As a valid sample,the wave propagation on the rectangular step is formulated by the present scheme,the modelled results are in better agreement with the experimental data than those of Kittitanasuan.  相似文献   
72.
病毒囊膜是包膜病毒除核衣壳外的另一主要成分。它主要由多种蛋白质构成,称为膜蛋白,膜蛋白以单聚体,二聚体,三聚体,甚至更多的聚合体的形式存在,具有中和抗体,与靶细胞受体结合,凝集红细胞,诱导细胞融合等多种功能,膜蛋白可采用将其基因整合入载体,并在昆虫细胞,哺乳细胞或细菌中获得体外表达,膜蛋白可在去污剂的作用下形成蛋白质-脂-去污剂的复合物而被溶解,其中去污剂的临界微团浓度,亲水物-亲脂物平衡值,荷电、紫外穿透性等是选择合适去污剂的主要参考因子,溶解后的膜蛋白多利用聚丙烯酰胺凝胶电泳、凝胶过滤层析,离子交换层析,亲和层析,高效液相层析等技术并结合梯度离心;分级沉淀等进行分离、纯化。  相似文献   
73.
Sea surface temperatures (SSTs) in the southwestern South China Sea have been reconstructed for the past 160 ka using the Uk37 paleothermometer from the core MD01-2392. The temperature differences between glacial times (MISs 6 and 2) and interglacial times (MISs 5.5 and 1) are 2.2~2.5 ℃. Younger Dryas event during the last deglaciation was documented in both the planktonic foraminiferal δ18O and SST records. After MIS 5.5, SSTs displayed a progressive cooling from 28.6 to 24.5 ℃, culminating at the LGM. During this gradual cooling period, warm events such as MISs 5.3, 5.1 and 3 were also clearly documented. By comparison of SST between the study core and Core 17954, a pattern of low or no meridional SST gradients during the interglacial periods and high meridional SST gradients during the glacial periods was exhibited. This pattern indicates the much stronger East Asian winter monsoon at the glacial than at the interglacial periods. Spectral analysis gives two prominent cycles: 41 and 23 ka, with the former more pronounced, suggesting that SSTs in the southern SCS varied in concert with high-latitude processes through the connection of East Asian winter monsoon.  相似文献   
74.
凡纳滨对虾咸淡水养殖系统内细菌群落组成的PCR-DGGE分析   总被引:16,自引:0,他引:16  
采用PCR(polym erase chain reaction)-DGGE(denaturing grad ient gel electrophoresis)及传统的微生物培养方法对凡纳滨对虾Litopenaeus vannam ei咸淡水养殖系统内各种环境的细菌群落组成进行了比较研究。传统的微生物培养计数表明,从海湾水、养殖池水到对虾粪样,细菌和弧菌的数量表现出依次增加的趋势,粪样及肠壁中弧菌的数量高出外界水环境1—4个数量级。相对于外界水环境,粪样中有很高的芽孢杆菌孢子含量,但是肠壁定植细菌中不存在芽孢杆菌(孢子)。PCR-DGGE及聚类分析结果表明,从海湾沿岸、养殖池、对虾粪便到对虾肠壁,细菌群落的多样性由高到低,无论是在哪种环境,群落的优势种都十分明显,且只有2—4种。来自同一环境各样品间的细菌群落组成非常相似,来自不同环境的样品,其细菌群落组成差别较大。聚类图上各簇的排列顺序反映了样品在取样空间分布上的毗邻次序和它们的相似程度。  相似文献   
75.
为解决中低滩涂沉积的深厚软粘土层的输送难题,提出了一种清淤输泥的新方法,研制了能远距离输送高浓度、大粘度、低含水量的淤泥和软粘土的土方施工新设备:包括进料口、推送器、表面膜发生器、脉冲切割器、定位系统、智能控制系统、输料管道、管道浮子等。该设备具有能耗低,工效高,效益好;结构简单,故障少,运行维护方便;定位准确性高;自动化程度高等特点。可广泛应用于水利、环保、建筑工程中的淤泥和软粘土输送。  相似文献   
76.
浙江省海塘塘顶高程的确定   总被引:10,自引:1,他引:10  
介绍了浙江省海塘塘顶高程确定时所涉及的波浪爬高,越浪量等的计量,同时分析了1997年后浙江省海塘塘顶高程确定的方法,由已建海塘的塘顶高程分析可见,在背水坡和塘顶有保护措施的情况下,采用允许最大越波流量来控制海塘塘顶高程,可适当降低塘身高度。  相似文献   
77.
Data are presented indicating the complexity and highly variable response of beaches to cold front passages along the northern Gulf of Mexico, in addition to the impacts of tropical cyclones and winter storms. Within the past decade, an increase in the frequency of tropical storms and hurricanes impacting the northern Gulf has dramatically altered the long-term equilibrium of a large portion of this coast. A time series of net sediment flux for subaerial and nearshore environments has been established for a section of this coast in Florida, and to a lesser extent, Mississippi. The data incorporate the morphological signature of six tropical storms/hurricanes and more than 200 frontal passages.

Data indicate that (1) barrier islands can conserve mass during catastrophic hurricanes (e.g., Hurricane Opal, a strong category 4 hurricane near landfall); (2) less severe hurricanes and tropical storms can promote rapid dune aggradation and can contribute sediment to the entire barrier system; (3) cold fronts play a critical role in the poststorm adjustment of the barrier by deflating the subaerial portion of the overwash terrace and eroding its marginal lobe along the bayside beach through locally generated, high frequency, steep waves; and (4) barrier systems along the northern Gulf do not necessarily enter an immediate poststorm recovery phase, although nested in sediment-rich nearshore environments. While high wave energy conditions associated with cold fronts play an integral role in the evolution and maintenance of barriers along the northern Gulf, these events are more effective in reworking sediment after the occurrence of extreme events such as hurricanes. This relationship is even more apparent during the clustering of tropical cyclones.

It is anticipated that these findings will have important implications for the longer term evolution of barrier systems in midlatitude, microtidal settings where the clustering of storms is apparent, and winter storms are significant in intensity and frequency along the coast.  相似文献   

78.
The present note describes the microbial degradation of crude oil and hydrocarbons, and discusses the microbial purification of the sea. The study shows that, (1) The ability, of Gram-negative bacteria to degrade crude oil is higher than Gram-positive bacteria. Degradation by Flavobacterium spp. and Xanthomonas spp. is higher than others; (2) The ability of dif-  相似文献   
79.
用16S rRNA基因的内切酶图谱快速鉴别几种对虾病原菌   总被引:4,自引:0,他引:4  
坎普氏弧菌是青岛海洋大学生物系微生物实验室于1989-1990年自对虾养殖场中国对虾红腿病心脏及血淋巴中分离并鉴定的菌株,副溶血菌和溶藻胶弧菌两菌株于1994年9月得自中国科学院微生物研究所,为建立快速、准确的中国对虾病原菌的诊断技术,根据几种细菌的16SrRNA基因的序列,设计并合成该基因的多聚酶反应的引物PL1和PL2。并用该对引物分别从坎普氏弧菌、副溶血弧菌和溶藻胶弧菌的DNA要品中扩增出分  相似文献   
80.
南沙群岛海域水体漫衰减系数Kd(490)的特性研究   总被引:6,自引:1,他引:6  
对南沙群岛海区水体Kd(490)的特性进行了深入分析和探讨,研究结果表明:Kd(490)的垂直分布与同步测量的海水叶绿素a浓度的垂直分布有很强的相关性;Kd(490)的垂直分布反映出该区域海水存在较强的分层现象,研究中还发现上行辐亮度漫衰减系数KL(490)与Kd(490)垂直变化趋势相当一致,但是以二者达到最大值的深度为界,上层水体的KL(490)值稍大于Kd(490)值,在这一深度以下则相反。  相似文献   
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