Journal of Oceanology and Limnology - Blepharipoda liberate Shen is a commercially valuable seafood species that has important ecological significance in Shandong Province, China. Although B.... 相似文献
Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( Ho ) and expected heterozygosity ( He ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.
Journal of Geographical Sciences - In this study, we developed an energy security evaluation model (ESEM) from three dimensions, energy supply-transport security, safety of energy utilization, and... 相似文献
Journal of Geographical Sciences - Land use/land cover change (LUCC) has considerable impact on ecosystem services. It is essential to quantify the spatiotemporal heterogeneity of ecosystem... 相似文献
Existing spatial clustering methods primarily focus on points distributed in planar space. However, occurrence locations and background processes of most human mobility events within cities are constrained by the road network space. Here we describe a density-based clustering approach for objectively detecting clusters in network-constrained point events. First, the network-constrained Delaunay triangulation is constructed to facilitate the measurement of network distances between points. Then, a combination of network kernel density estimation and potential entropy is executed to determine the optimal neighbourhood size. Furthermore, all network-constrained events are tested under a null hypothesis to statistically identify core points with significantly high densities. Finally, spatial clusters can be formed by expanding from the identified core points. Experimental comparisons performed on the origin and destination points of taxis in Beijing demonstrate that the proposed method can ascertain network-constrained clusters precisely and significantly. The resulting time-dependent patterns of clusters will be informative for taxi route selections in the future. 相似文献
Understanding scale effects is important and indispensable for geography studies. However, spatial and spatiotemporal statistical tools for measuring the operational scales of different processes are rather limited. This article extends the popular geographically and temporally weighted regression (GTWR) model to consider operational scale effects by proposing multiscale GTWR (MGTWR), which offers a flexible and scalable framework for identifying and analysing multiscale processes by specifying flexible bandwidths for various covariates. Then, MGTWR is employed to explore spatiotemporal variations and how influential factors are associated with housing prices in Shenzhen. This article attempts to extend GTWR to MGTWR in consideration of scale effects, thereby highlighting the importance of different levels of spatiotemporal heterogeneity. Furthermore, the empirical results of this study can provide valuable policy implications for real estate development in areas where urban planning should address multiscale effects in both temporal and spatial dimensions. 相似文献
Urban hierarchies are closely related to economic growth, urban planning and sustainable urban development. Due to the limited availability of reliable statistical data at fine scales, most existing studies on urban hierarchy characterization failed to capture the detailed urban spatial structure information. Previous studies have demonstrated that night time light data are correlated with many urban socio-economic indicators and hence can be used to characterize urban hierarchies. This paper presents a novel method for studying urban hierarchies from night time light data. Night time light data were first conceptualized as continuous mathematical surfaces, termed night time light surfaces. From the morphology of these surfaces the corresponding surface networks were derived. Hereafter, a night time light intensity (NTLI) graph was defined to describe the morphology of the surface network. Then, structural similarity between the night time light surfaces of any two different cities was calculated via a threshold-based maximum common induced graph searching algorithm. Finally, urban hierarchies were defined on the basis of the structural similarities between different cities. Using the 2015 annual NPP-VIIRS night time light data, the urban hierarchies of 32 major cities in China were successfully examined. The results are highly consistent with the reference urban hierarchies. 相似文献
Understanding the spatial scale sensitivity of cellular automata is crucial for improving the accuracy of land use change simulation. We propose a framework based on a response surface method to comprehensively explore spatial scale sensitivity of the cellular automata Markov chain (CA-Markov) model, and present a hybrid evaluation model for expressing simulation accuracy that merges the strengths of the Kappa coefficient and of Contagion index. Three Landsat-Thematic Mapper remote sensing images of Wuhan in 1987, 1996, and 2005 were used to extract land use information. The results demonstrate that the spatial scale sensitivity of the CA-Markov model resulting from individual components and their combinations are both worthy of attention. The utility of our proposed hybrid evaluation model and response surface method to investigate the sensitivity has proven to be more accurate than the single Kappa coefficient method and more efficient than traditional methods. The findings also show that the CA-Markov model is more sensitive to neighborhood size than to cell size or neighborhood type considering individual component effects. Particularly, the bilateral and trilateral interactions between neighborhood and cell size result in a more remarkable scale effect than that of a single cell size. 相似文献
The discovery of spatial clusters formed by proximal spatial units with similar non-spatial attribute values plays an important role in spatial data analysis. Although several spatial contiguity-constrained clustering methods are currently available, almost all of them discover clusters in a geographical dataset, even though the dataset has no natural clustering structure. Statistically evaluating the significance of the degree of homogeneity within a single spatial cluster is difficult. To overcome this limitation, this study develops a permutation test approach Specifically, the homogeneity of a spatial cluster is measured based on the local variance and cluster member permutation, and two-stage permutation tests are developed to determine the significance of the degree of homogeneity within each spatial cluster. The proposed permutation tests can be integrated into the existing spatial clustering algorithms to detect homogeneous spatial clusters. The proposed tests are compared with four existing tests (i.e., Park’s test, the contiguity-constrained nonparametric analysis of variance (COCOPAN) method, spatial scan statistic, and q-statistic) using two simulated and two meteorological datasets. The comparison shows that the proposed two-stage permutation tests are more effective to identify homogeneous spatial clusters and to determine homogeneous clustering structures in practical applications. 相似文献
Natural Resources Research - Quantitative prediction of concealed mineralization is always confronted with difficulties in comprehensive analysis between 2D and 3D data and between qualitative and... 相似文献