长牡蛎碱性磷酸酶的分离纯化及部分性质研究   总被引：5，自引：1，他引：5  

李兴暖  韩雅莉  肖湘  邓瑞鹏  周小朋  管云雁  黄长江 《台湾海峡》2003,22(4):482-486

本文以长牡蛎为酶源提取材料,用正丁醇抽提,硫酸铵分级分离,DEAE-52和Sephadex G-100柱层析,得到一定纯度的碱性磷酸酶,提纯倍数为61.96,比活力达1.326U/mg.该酶的最适pH值9.6,最适温度38℃,初速度6.8μmol/(dm~3·min),米氏常数Km值为1.30mmol/dm~3.实验结果表明,Mg~(2+)对该酶有明显的激活作用;Zn~(2+)、Cu~(2+)、Hg~(2+)对该酶有明显的抑制作用.  相似文献   

东海大陆架沉积物中的放射虫   总被引：5，自引：0，他引：5  

谭智源  宿星慧 《海洋科学集刊》1982,(19):129-232

利用海洋沉积物中放射虫的研究资料来判別海底地层和解决与海洋学有关的问題, 系探索海洋资源的手段之一。近年来,一些国家对世界大洋的调查研究工作进展很快，目前,除北冰洋外；几乎对各大洋的大部分海域都进行了钻探取样。其中,用放射虫沉积岩样结合地层学进行分析划带的工作已有很大进展。东海大陆架范围辽阔,资源丰富,亟待开发利用,但对其海底情况过去了解较少。为配合当前渔场调查和海底矿产资源的探测,对海底沉积物及有关方面进行调查研究是急需的。本文就东海大陆架部分地区(东经121°30′-127°,北纬26°30′-32°30′；图1)海底沉积物中的放射虫进行研究整理、以供作海洋开发的基础资料。  相似文献   

高压技术在深海沉积物兼性嗜压菌的筛选和鉴定中的应用     

游志勇  汤熙翔  肖湘 《台湾海峡》2007,26(4):555-561

通过自行改进的高压培养罐及高压设备,对深海沉积物进行可培养微生物的筛选,获得6株具有较强耐受压力的细菌.16SrDNA的测序结果表明这些细菌分别属于6个不同的菌属.压力生长试验的结果表明这6株细菌在40MPa的条件下仍然具有较强的生长能力,属于兼性嗜压菌.对不同压力下生长的细菌做显微镜检,结果显示,除了一株芽孢杆菌在40MPa下的菌体形态发生了明显的变化外,其它5株细菌在压力条件下的菌体的分裂均没有受到明显的影响.  相似文献   

养殖对虾病毒性疾病的细菌并发症防治研究   总被引：1，自引：0，他引：1  

苏国成  陈金翠 《台湾海峡》1996,15(2):200-204

本文研究报道了发病对虾肝胰腺分离菌物敏感性，采用防台细菌人工感染方法进行药物饲料的筛选，在实验室及养殖池中进行药物饲料的防治效果试验。结果表明，在对虾病毒流行期间，采用药物饲料可有效防止对虾爆发性大量死亡，提高对虾存活率。  相似文献   

The relationship between tidal current field and sediment transport in the Huanghai Sea and the Bohai Sea   总被引：2，自引：0，他引：2  

Dong Lixian  Su jilan  Wang Kangshan 《海洋学报(英文版)》1989,8(4):587-600

-Based on a two-dimensional numerical M2 tidal model of the Huanghai and Bohai Seas, sediment transport of particles with different sizes in the computational area is given. It is concluded that tidal currents play an important role in sediment transport and deposition in the Huanghai and Bohai Seas.  相似文献   

Studies on ultrastructure of oocyte in process of maturing in pen shell     

Fang Yongqiang  Qi Xiang 《海洋学报(英文版)》1988,7(3):459-472

The present paper deals with the ultrastructural changes of oocyte in the process of maturing in pen shell Atrinapectinata Linne. When it develops from the antecedent growth period to the accelerating growth period the annulate lamellae emerge, the RER better develop, the yolk granules with single and double layer membrane and are visible. In the well-developed mitochondria there is yolk stored. The Golgi vacuoles are brewing yolk granules. At the mature period the annulate lamellae and the Golgi bodies disappear, and there are only a few RER and mitochondria with poor cristae in the cytoplasm. All these might be well used for judging the maturity of the ootids in the process of the artificial propagation.  相似文献   

Simulations of Annual Cycle of Phytoplankton Production and the Utilization of Nitrogen in the Yellow Sea     

Tian?TianEmail author  Hao?Wei  Jian?Su  Changsoo?Chung 《Journal of Oceanography》2005,61(2):343-357

A nutrient dynamic model coupled with a 3D physical model has been developed to study the annual cycle of phytoplankton production in the Yellow Sea. The biological model involves interactions between inorganic nitrogen (nitrate and ammonium), phosphate and phytoplankton biomass. The model successfully reproduces the main features of phytoplankton-nutrient variation and dynamics of production. 1. The well-mixed coastal water is characterized by high primary production, as well as high new production. 2. In summer, the convergence of tidal front is an important hydrodynamic process, which contributes to high biomass at frontal areas. 3. The evolution of phytoplankton blooms and thermocline in the central region demonstrate that mixing is a dominant factor to the production in the Yellow Sea. In this simulation, nitrate- and ammonium-based productions are estimated regionally and temporally. The northern Yellow Sea is one of the highly ranked regions in the Yellow Sea for the capability of fixing carbon and nitrogen. The annual averaged f-ratio of 0.37 indicates that regenerated production prevails over the Yellow Sea. The result also shows that phosphate is the major nutrient, limiting phytoplankton growth throughout the year and it can be an indicator to predict the bloom magnitude. Finally, the relative roles of external nutrient sources have been evaluated, and benthic fluxes might play a significant role in compensating 54.6% of new nitrogen for new production consumption.  相似文献   

冲绳海槽中段沉积岩芯碳酸盐和烧失量的古环境意义     

吴世迎  刘焱光  王湘芹  白黎明  吴军瑞 《海洋科学进展》2001,19(2):17-24

对冲绳海槽中段 3个沉积岩芯碳酸盐和烧失量的系统分析得知 ,岩芯沉积物的CaCO3含量一般 >1 0 % ,最高含量接近 30 % ,烧失量大都在 1 0 %～ 30 %之间变化 ,反映了该海区处于溶跃面之上钙质生物极为发育的生物地球化学环境。结合δ1 8O曲线所反映的气候环境变化 ,探讨了 1 2 0kaB .P .以来碳酸盐旋回的“大西洋型”演变特点 ,同时对该区沉积地层中的火山热液活动印记作了一定的探讨。  相似文献   

济州岛南部海区的双跃层现象     

苏育嵩  李凤岐  马鹤来  钱清瑛 《中国海洋大学学报(自然科学版)》1989,(Z1)

本文分析了济州岛南部区域温度双跃层现象的类型及其成因。特别阐明了双跃层的波状现象,指出:强流锋区的侧向效应,黑潮次—中层混合水的爬升,黄海冷水团边缘密度环流引起的侧向流动,以及上、下层流速、流向不一致引起的剪切作用,是导致双跃层波状现象的主要原因。发现上、下跃层之间存在着“跃层间环流”,这是一个有趣的海洋学现象,它将导致双扩散的发生和有利于双跃层的维持。  相似文献   

Practical implementation of Hilbert-Huang Transform algorithm   总被引：12，自引：0，他引：12  

Huang Daji  Zhao Jinping  Su Jilan 《海洋学报(英文版)》2003,22(1):1-14

Hilbert-Huang Transform (HHT) is a newly developed powerful method for nonlinear and non-stationary time series analysis. The empirical mode decomposition is the key part of HHT, while its algorithm was protected by NASA as a US patent, which limits the wide application among the scientific community. Two approaches, mirror periodic and extrema extending methods, have been developed for handling the end effects of empirical mode decomposition. The implementation of the HHT is realized in detail to widen the application. The detailed comparison of the results from two methods with that from Huang et al. (1998, 1999), and the comparison between two methods are presented. Generally, both methods reproduce faithful results as those of Huang et al. For mirror periodic method (MPM), the data are extended once forever. Ideally, it is a way for handling the end effects of the HHT, especially for the signal that has symmetric waveform. The extrema extending method (EEM) behaves as good as MPM, and it is better t  相似文献