夜光藻的室内培养   总被引：3，自引：1，他引：3  

吴玉霖  周成旭  张永山 《海洋与湖沼》1994,25(2):165-167

夜光藻的室内培养对于研究夜光藻的生理生态特性及其增殖机理是一个关键性的环节。通过试验，发现在实验室条件下以扁藻作为饵料，夜光藻能良好的生长和繁殖，从而为夜光藻的室内培养解决了关键性的饵料问题，实现了国内夜光藻室内的长期培养。  相似文献   

二色桌片参酶解液对转化细胞的效应   总被引：3，自引：2，他引：3  

陈正明  陈玉强  曹顺达  吴乔  苏文金 《台湾海峡》1997,16(3):270-274,T001

用二色桌片参（Mensamariaintercedens）酶解液处理转化的人胚肺成纤维细胞（NLF）后，细胞生长受到显著抑制，抑制率达64．84％，光镜下细胞透光性加强，铺展较好，细胞核形更为规则，核畸形现象减少；线粒体结构趋于正常；软琼脂集落实验表明，细胞集落形成率由23ｘ10－5下降至12．5ｘ10－5，裸小鼠致瘤实验显示抑瘤率为82．39％，差异显著（p＜5％）。结果提示，二色桌片参酶解液可能逆转转化的HLF细胞的恶性表型，对其有一定的诱导分化作用。  相似文献   

A proposed directional function and wind-wave directional spectrum   总被引：4，自引：0，他引：4  

Wen Shengchang  Wu Kejian  Guan Changlong  Sun Shicai  Zhang Dacuo 《海洋学报(英文版)》1995,14(2):155-166

Ａｐｒｏｐｏｓｅｄｄｉｒｅｃｔｉｏｎａｌｆｕｎｃｔｉｏｎａｎｄｗｉｎｄ－ｗａｖｅｄｉｒｅｃｔｉｏｎａｌｓｐｅｃｔｒｕｍ￥ＷｅｎＳｈｅｎｇｃｈａｎｇ；ＷｕＫｅｊｉａｎ；ＧｕａｎＣｈａｎｇｌｏｎｇ；ＳｕｎＳｈｉｃａｉａｎｄＺｈａｎｇＤａｃｕｏ（Ｒｅｃｅｉ...  相似文献   

东海大规模赤潮危害影响的实验研究——对黑褐新糠虾和卤虫的毒性效应     

颜天  张艺  韩刚  陈洋  周名江 《海洋科学集刊》2007,(48):166-175

The effects of Prorocentrum donghaiense,Karenia mikimotoi,Alexandrium catenella,the causative species of Harmful Algal Blooms in the East China Sea,on the survival and growth of Neomysis awatschensis and Artemia salina were investigated.And the toxicity of eleven strains of Alexandrium----A.lusitanicum(AL-1)、A.minutum(AM-1)、A.spp.(A-K)、A.tamarense(ATHK,ATCI02,ATCI03,AT5-1,AT5-3,AT-6)、A.affine(AS-1,AC-1) to the two above organisms were compared. The results showed that P.donghaiense and K.mikimotoi had no adverse effect on the survival and growth of N.awatschensis and A.salina at 96h.However,the survival rate of N.awatschensis and A.salina declined linearly with the increasing density of A.catenella,and the 96h LC₅₀ for N.awatschensis and A.salina was 4500 and 460 cells/mL,respectively, Five strains of Alexandrium----A.spp,A.lusitanicum (AL-1),A.tamarense(AT5-3,AT-6) and A.affine (AS-1)at the density of 4000cells/mL and 96h had no adverse impacts on N.awatschensis. But other six strains of Alexandrium a.Tamarense(AT5-1,ATHK,ATCI02,ATCI03),A.affine(AC-1) and A.minutum(AM-1)decreased the survival fate of N.awatschensis significantly. Two strains of Alexandrium----A.lusitanicum(AL-1) and A.tamarense(AT-6),at the density of 500cells/mL. had no adverse effects on A.salina in 96h;while the other Alexandrium strains all had adverse effeets on the survival of a.salina, A.salina could increase its length with the increasing density of P.donghaiense,K.mikimotoi and A.luxitanicum. The results indicate that P.donghaiense and K.mikimotoi have nio significant effect on the survival of crustaeean zoop lankton in a short period. But several Alexandrium specised/strains could influence the survival of crustacean zooplankton likely by non-PSP substances. A.salina is shown to be more sensitive than N.awatschensis as test organisms to harmful species.  相似文献   

Outer wave number spectrum and statistical distribution of apparent wave number vector     

J. -B. Song  K. -J. Wu  Y. -H. Wu 《Ocean Engineering》2003,30(3):401-412

Based on the ray theory and Longuet-Higgins’s linear model of sea waves, the joint distribution of wave envelope and apparent wave number vector is established. From the joint distribution, we define a new concept, namely the outer wave number spectrum, to describe the outer characteristics of ocean waves. The analytical form of the outer wave number spectrum, the probability distributions of the apparent wave number vector and its components are then derived. The outer wave number spectrum is compared with the inner wave number spectrum for the average status of wind-wave development corresponding to a peakness factor P = 3. Discussions on the similarity and difference between the outer wave number spectrum and inner one are also presented in the paper.  相似文献   

Algorithm for HF radar vector current measurements   总被引：1，自引：0，他引：1  

Lei Liu  Xiongbin Wu  Feng Cheng  Shaolin Yang  Hengyu Ke 《Journal of Oceanography》2007,63(1):47-66

A new algorithm is proposed, called the stream function method (SFM) for producing vector current maps from radial data measured by dual-site high frequency surface wave radar (HFSWR). In SFM, a scalar stream function is constructed under some oceanographic assumptions. The function describes the two-dimensional (2-D) ocean surface water motion and is used to obtain the distribution of vector currents. The performance of SFM is evaluated using simulated radial data, which demonstrates that SFM has advantages over typical vectorial combination methods (VCM) both in error acceptance and robustness, and excels another method based on least-squares fitting (LSF) in recovering the complicated current models. Furthermore, SFM is capable of providing the total currents based on radials from single-site radar. We also test the assumptions of horizontal non-divergence in the simulation. The new algorithm is applied to the field experiment data of Wuhan University’s ocean state measuring and analyzing radar (OSMAR), collected in the coastal East China Sea during April 11–17, 2004. Quantitative comparisons are given between radar results by three current algorithms and in-situ current meter measurements. Preliminary analysis of the vertical current shear is given based on the current meter measurements.  相似文献   

几种红藻和蓝藻的光合作用色素   总被引：2，自引：0，他引：2  

仵小南  张锦东  张学成  臧汝波 《海洋科学》1992,16(6):39-44

分离纯化出几种海产红藻和一种蓝藻的光合作用色素,并测定了它们的化学性质和光谱学性质。这些藻类是3种红藻:多管藻(Polysiphonia urceolata)、橡叶藻(Phycodrys sp.)和条斑紫菜(Porphyra yezoensis);蓝藻:钝顶螺旋藻(Spirulina platensis)。用羟基磷灰石柱层析法从上述藻类中分离到几种不同的藻胆蛋白。经SDS-PAGE及光谱测定,发现条斑紫菜中的藻红蛋白不同于其它两种红藻。而橡叶藻中存在的两条藻红蛋白也有差异,条斑紫菜和钝顶螺旋藻中的两种别藻蓝蛋白之间也有区别。叶绿素分析表明,钝顶螺旋藻中叶绿素a的含量高于红藻中叶绿素a的含量。  相似文献   

台湾海峡西部海域软质海底多毛类的生态   总被引：2，自引：0，他引：2  

吴启泉 《台湾海峡》1993,12(4):324-334

本文根据１９８４－１９８５年４航次底栖生物调查资料，首次全面地研究了台湾海峡西部海域多毛类的种类组成，数量分布及其与环境因子的关系。结果表明，该海域多毛类４０４种，习见种１９１种，区系属亚热带性质；平均生物量４．３３ｇ／ｍ＾２，平均栖息密度６８个／ｍ＾２；有沿岸性和外洋性两种明显不同的生态类型，前者位于沿岸水系常年控制海域，种类多，数量较大，后者位于南部粗粒沉积物分布区，种类较少；多毛类高数量区与  相似文献   

The effects of sediment from an industrial outfall area on a marine planktonic ecosystem     

Wu Jinping 《海洋学报(英文版)》1990,9(1):151-158

-Sediment from Xiamen industrial area was added to marine ecosystem enclosures to test the effects on the planktonic communities in Xiamen Bay, China, in April, 1985. Sediments were added at two concentrations, 11.2 and 112 ppm dry wt. respectively. The species of phytoplankton did not change as compared with the control, but the number of diatoms markedly decreased in the sediment treated enclosures. A suppression of photosynthesis was presumed to be due to light attenuation by the added sediment. Microflagellates, zooplankton and bacteria were resistant to sediment increase in water column.  相似文献   

裙带菜原生质体的分离和培养   总被引：1，自引：0，他引：1  

吴少波 《中国海洋大学学报(自然科学版)》1988,(4)

本文以裙带菜(Undaria pinnatifida(Harv.)Suringar)为材料,进行原生质体分离和培养研究。取得以下结果: 1、使用海螺酶(sea snail enzymes)和纤维素酶(cellulase,Onozuka R-10),酶解裙带菜细胞壁,在一定的条件下,能够大量地分离成活的裙带菜原生质体。 2、实验表明,氯化钠可作为分离裙带菜原生质体研究中的一种理想的渗透刑。 3、荧光增白剂染色镜检法可作为鉴定裙带菜原生质体的一种辅助方法,并适用于原生质体再生壁的观察。 4、光照(2000—2500Lux)能促使原生质体再生细胞壁,含有蔗糖(W/V,1％)的培养液有助于原生质体再生细胞壁。 5、分离的裙带菜原生质体,经培养,已能长成幼体。  相似文献