冲绳日本绒螯蟹线粒体DNA 12S rRNA序列的研究   总被引：2，自引：0，他引：2  

张秀梅  高天翔  王溪宏 《广东海洋大学学报》2000,20(2):11-14

采集日本冲绳源河川和边野古川的日本绒螯蟹样品 ,参考果蝇与蚤状氵蚤线粒体 DNA12 Sr RNA基因片段序列进行了其相同片段的引物设计、PCR扩增及序列测定。结果表明冲绳两河川 4只日本绒螯蟹的碱基序列完全相同 ,为 4 58bp,其 A、T、G、C含量分别为 16 0 bp(34.94 % )、 179bp(39.0 8% )、4 9bp(10 .70 % )、70 bp(15.2 8% ) ,同果蝇与蚤状氵蚤相同基因片段的序列含量相似。  相似文献   

Molecular phylogeny of familiar flatfish species （Pleuronectidae） in the northwestern Pacific Ocean based on mitochondrial cytochrome oxidase Ⅰ gene     

ZHANG Yan XIAO Yongshuang Gao Tianxiang 《海洋学报(英文版)》2008,27(2)

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巢湖地学实习基地数字化平台的建立     

刘天翔  刘国生  苏蓉 《地理空间信息》2009,7(3):97-98

利用GIS工具（MapInfo）对地图（例如巢湖地学基地）进行矢量化，打下建立数字化平台的基础。再根据断层，等高线等分布资料，利用GIS的空间分析功能描绘出整个巢湖地学基地地形分布图。对地形分布图加入它的属性，从而可以比较直观系统地对其分析．也方便了地学工作者。  相似文献   

Extensive genetic divergence among Diptychus maculatus populations in northwest China     

孟玮  杨天燕  海萨  马燕武  蔡林钢  马徐发  高天翔  郭焱 《中国海洋湖沼学报》2015,33(3):577-584

D. maculates is a kind of specialized Schizothoracinae fish has been locally listed as a protected animal in Xinjiang Province,China. Ili River located in north of Tianshan Mountain and Tarim River located in north of Qinghai-Tibetan Plateau were two main distribution areas of this fish. To investigate the genetic diversity and genetic structure of D. maculates,four populations from Tarim River system and two populations from Ili River system were collected in this study. A 570-bp sequence of the control region was obtained for 105 specimens. Twenty-four haplotypes were detected from six populations,only Kunes River population and Kashi River population shared haplotypes with each other. For all the populations examined,the haplotype diversity(h) was 0.904 8±0.012 6,nucleotide diversity(π) was 0.027 9±0.013 9,and the average number of pairwise nucleotide differences(k) was 15.878 3±7.139 1. The analysis of molecular variance(AMOVA) showed that 86.31% of the total genetic variation was apportioned among populations,and the variation within sampled populations was 13.69%. Genetic differences among sampled populations were highly significant. F st statistical test indicated that all populations were significantly divergent from each other(P 0.01). The largest F st value was between Yurungkash River population and Muzat River population,while the smallest F st value was between Kunes River population and Kashi River population. NJ phylogenetic tree of D-loop haplotypes revealed two main clades. The neutrality test and mismatch distribution analysis suggested that the fish had went through a recent population expansion. The uplift of Tianshan Mountain and movement of Qinghai-Tibetan Plateau might contribute to the wide genetic divergence of D. maculates in northwest China.  相似文献   

Genetic variation of the small yellow croaker (Larimichthys polyactis) inferred from mitochondrial DNA provides novel insight into the fluctuation of resources     

Jian Zheng  Tianxiang Gao  Yunrong Yan  Na Song 《海洋学报(英文版)》2022,41(11):88-95

The small yellow croaker (Larimichthys polyactis) belongs to the family Sciaenidae, which is an offshore warm fish species and widely distributed in the western Pacific. In this study, the variation of genetic diversity and genetic differentiation among L. polyactis populations was analyzed by mitochondrial DNA control region. A total of 110 polymorphic sites were checked, which defined 134 haplotypes. High level of haplotype diversity (h=0.993±0.002) was detected in the examined range. Population genetic structure analyse (analysis of molecular variance, F_st) showed there were high gene flow among L. polyactis populations. The result showed that there were relatively high genetic diversity and low genetic differentiation among the Yellow Sea and the East China Sea populations, which can be attributed to diverse habitats, wide distribution range and high mutation rate of control region. Using phylogenetic methods, coalescent analyses (neutrality tests, mismatch distribution analysis, Bayesian skyline analyses) and molecular dating interpreted in conjunction with paleoclimatic and physiographic evidence, we inferred that the genetic make-up of extant populations of L. polyactis was shaped by Pleistocene environmental impacts on the historical demography of this species. Besides, relatively constant genetic diversity and larger effective population size were detected in recent L. polyactis population. The result showed that the fishing policy certainly, such as the summer closed fishing, played a role in protecting resources of L. polyactis. This study can offer a wealth of biological novelties which indicates genetic structure of L. polyactis population and provides the foundation for resources protection and policy setting.  相似文献   

Use of tetracycline hydrochloride and alizarin complexone for immersion marking black rockfish Sebastes schlegelii     

吕红健  张秀梅  付梅  席丹  高天翔 《中国海洋湖沼学报》2014,32(4):810-820

We tested the utility of chemical marking techniques in the juvenile black rockfish Sebastes schlegelii. Juveniles(30–40 mm total length) were immersed in a range of tetracycline hydrochloride(TC) solutions at concentrations ranging from 300 to 500 mg/L, and alizarin complexone(ALC) solutions at concentrations ranging from 200 to 400 mg/L in filtered sea water(salinity of 30) for 24 h, respectively. Otoliths(sagittae, asteriscus), scales, fin rays(dorsal, pectoral, ventral, anal, and caudal fin rays), and fin spines(dorsal, ventral, and anal fin spines) were sampled and used to detect fl uorescent marks after a 60-day growth experiment. With the exception of 300 mg/L TC, acceptable marks were produced in the otoliths and fin spines by all concentrations of TC and ALC. In particular, we observed clearly visible marks in the sagittae, asteriscus, and fin spines under normal light at concentrations of 200–400 mg/L, 250–400 mg/L, and 250–400 mg/L ALC, respectively. Scales and fin rays had acceptable marks at much higher concentrations(≥350 mg/L TC, ≥250 mg/L ALC for scales and ≥350 mg/L TC, ≥300 mg/L ALC for fin rays). The best mark quality(i.e., acceptable marks were observed in all sampled structures after immersion marking) were obtained following immersion in TC at between 350–500 mg/L, and ALC between 300–400 mg/L. In addition, there was no significant difference in survival and growth of TC and ALC marked fish compared to their controls up to 60 days post-marking( P 0.05).  相似文献   

基于模型流的资源环境模型复合语言与应用     

王晨亮  岳天祥  范泽孟 《地球信息科学学报》2014,(1):31-38

资源环境领域的研究对象常被抽象为若干子模型复合而成的复杂模型,如何有效地集成这些子模型成为资源环境模型集成的主要内容。随着各种地学相关问题的日益复杂和多样化,传统的建模语言与友好易用的图形建模等集成建模方式已无法满足大规模高复杂的模型构建需求。本文在已有的相关理论基础上,针对复合模型构建的灵活性和复杂性之间的矛盾,将模型复合模式的本质抽象化,提出资源环境"模型流"的形式化语言描述,实现了模型流建模环境。应用实例表明,基于模型流的模型构建方式采用简洁的符号表示,避免了繁琐的图形构建环节,可快速灵活地构建高复杂度模型。模型流的提出为模型复合的调度性能优化和智能自动化集成等进一步的研究奠定了基础。  相似文献   

基于灰色定权聚类的江苏省工业节能减排评价研究     

徐沛勣  姚天祥 《南京气象学院学报》2014,6(4):374-379

采用灰色定权聚类分析法,对江苏省近年来的工业节能减排情况进行评价.结果表明：2000年以及2009-2011年的节能减排效果较好,2002-2008年的节能减排效果一般,2001年的节能减排效果较差.另外,还结合各项指标对评价结果进行分析,并有针对性地提出有关江苏省节能减排措施的建议.  相似文献   

空间异质性定量研究方法   总被引：8，自引：2，他引：6  

岳天祥 《地球信息科学学报》1999,1(2):75-79

空间异质性是景观生态学定量化研究的核心,其定量研究方法包括景观综合图、数学模型和地学信息图谱.本文概括总结了景观综合图的内容和定量化指标,分析了这种定量化方法的优势和局限;对数学模型的复杂性、优点和与景观综合图的互补性作了简要分析,指出了数学模型研究需要进一步探讨和重视的问题;通过对地学信息图谱三个阶段(景观制图实验,图谱概念的提出和地学信息图谱理论的形成)的回顾和对其特点的分析,我们认为地学信息图谱综合了景观综合图和抽象的数学模型可以提供的互补信息,吸收了两者的优点,是空间异质性的综合定量研究方法.  相似文献   

第二亚欧大陆桥的构成及其国际环境     

岳天祥 《地球信息科学学报》2000,2(4):9-13

本文将第二亚欧大陆桥描述为欧洲联盟网与中国东部网之间的连接,据此描绘了欧洲联盟网和中国东部网的构成,分析了亚洲、尤其是中亚地区围绕交通运输的国际合作形势和生态环境状况,阐述了泛欧洲交通与环境合作的内容和进程.  相似文献