菲律宾蛤仔(Ruditapes philippinarum)蛋白聚糖的分离提取及其抗肿瘤活性的初步研究   总被引：1，自引：0，他引：1  

张莉 《海洋与湖沼》2007,38(1):62-68

采用凝胶过滤层析、SDS-PAGE电泳、Bradford法和苯酚-硫酸法等生物化学方法对菲律宾蛤仔中的蛋白聚糖(PG1和PG2)进行分离纯化,研究其理化性质。采用MTT方法研究蛋白聚糖对人肝癌细胞SMMC-7721和人正常肝细胞HL-7702生长的影响。通过小鼠体内实验研究蛋白聚糖的抗肿瘤活性和对荷瘤小鼠免疫功能的影响。结果表明,PG1分子量大约为20kDa,多糖含量为70.09%,蛋白质含量为15.4%。PG2的多糖含量为82.53%,蛋白质含量为7.26%。PG1在200μg/ml浓度时对SMMC-7721的生长抑制率为73.30%,随浓度的升高,抑制率并未发生明显改变。PG2对SMMC-7721的生长抑制作用呈剂量依赖性,在500μg/ml的浓度下,其抑制效果与PG1的抑制效果相当。在同样浓度下,PG1对人正常肝细胞的生长和功能没有明显影响。PG1呈剂量-效应关系明显增加荷瘤小鼠脾指数,促进荷瘤小鼠脾淋巴细胞转化,且没有降低体重及其他毒副作用。PG1对小鼠S180肉瘤的抑制作用可能是通过直接杀伤肿瘤细胞的细胞毒作用和提高小鼠细胞免疫水平来实现。  相似文献   

海浪日最大波高序列的一种标度性质   总被引：1，自引：0，他引：1  

尉华  沈正 《海洋湖沼通报》1994,(4):1-8

应用重标度分析方法研究小麦岛海洋观测站１９６１－１９８０年二十年海浪日最大波高序列时发现，该序列并不是相互独立的，而是具有记忆性和持久性，详细的论证表明分式布朗运动模型是描述海浪日最大波高序列的一个较好的模型，同时，应用分式布朗运动模型我们找到了存在于海浪日最大波高序列中的一种标度性质－累积离差（ｔ）的统计自仿射性。  相似文献   

用16S rRNA基因的内切酶图谱快速鉴别几种对虾病原菌   总被引：4，自引：0，他引：4  

孔杰  刘萍  张岩  杨丛海  叶军  徐怀恕  郭华荣 《海洋与湖沼》1997,28(5):449-452

坎普氏弧菌是青岛海洋大学生物系微生物实验室于１９８９－１９９０年自对虾养殖场中国对虾红腿病心脏及血淋巴中分离并鉴定的菌株，副溶血菌和溶藻胶弧菌两菌株于１９９４年９月得自中国科学院微生物研究所，为建立快速、准确的中国对虾病原菌的诊断技术，根据几种细菌的１６ＳｒＲＮＡ基因的序列，设计并合成该基因的多聚酶反应的引物ＰＬ１和ＰＬ２。并用该对引物分别从坎普氏弧菌、副溶血弧菌和溶藻胶弧菌的ＤＮＡ要品中扩增出分  相似文献   

几株原甲藻核糖体大亚基RNA基因的部分克隆及序列分析   总被引：1，自引：0，他引：1       下载免费PDF全文

王波  米铁柱  吕颂辉  孙军  李荣秀  于志刚 《海洋学报》2007,29(1):120-126

对7株赤潮原甲藻28S rDNA 5'端部分序列进行扩增、克隆和序列测定,并从GeneBank上获取14个原甲藻28S rDNA序列,用NJ法和ME法构建了原甲藻属的系统树,并对序列进行分析.结果表明,7株原甲藻28S rDNA扩增序列长度为950~958 bp,通过NJ法和ME法构建的系统树完全一致.大部分分离自不同海域的同种原甲藻的序列高度保守,而不同种间在序列高变区却有较大的差异.但来自南海海域的海洋原甲藻(Prorocentrum micans)与分离自其他海域的株系序列差异较大,甚至超出了有些种间的差异.由28S rDNA高变区获得的序列,有望成为浮游植物特异性分子探针设计的良好靶区域.  相似文献   

中国沿海亚历山大藻(Alexandrium)核糖体rDNA部分序列分析及该藻属分子系统进化研究   总被引：4，自引：1，他引：4  

唐祥海  于仁成  颜天  王云峰  周名江 《海洋与湖沼》2006,37(6):529-535

分析了几株自南海及东海分离的亚历山大藻的rDNA部分序列信息,其中包括核糖体大亚基(LSU)rDNA的5′端D1-D2区序列,以及5.8SrDNA和ITS区序列;同时也对实验室保种的部分来自其它国家和地区的亚历山大藻相关序列进行了测序和分析,并以此作为序列分析中的参考。采用ClustalX及MEGA2软件对所得到的序列信息进行了综合分析与对比。结果表明,分离自南海的塔玛亚历山大藻(Alexandriumtamarense)和分离自东海的链状亚历山大藻(A.catenella),即便是在ITS区和LSUrDNA等高变区,其序列信息也完全一致。与基因库中搜索到的其它亚历山大藻rDNA序列信息相比较,中国沿海的塔玛/链状亚历山大藻序列更接近于塔玛复合种的“亚洲温带”基因型。对于分离自南海的另外两株未定种的亚历山大藻,通过对比序列信息,发现它们与相关亚历山大藻(A.affine)非常接近。分离于我国台湾地区的微小亚历山大藻(A.minutum)在序列上与分离自新西兰的藻株相似,而与分离自欧洲的微小亚历山大藻藻株相差较大。中国沿海亚历山大藻rDNA序列信息的获得为针对有毒藻种设计特异性核酸探针,发展灵敏快速的生物检测技术奠定了基础。  相似文献   

Comparative research on karyotype in three species of Arcidae     

Zheng Jiasheng  Wang Meilin  Guo Danhong  Xu Ximin  Gao Qinglan 《海洋学报(英文版)》1996,15(4):529-538

ＣｏｍｐａｒａｔｉｖｅｒｅｓｅａｒｃｈｏｎｋａｒｙｏｔｙｐｅｉｎｔｈｒｅｅｓｐｅｃｉｅｓｏｆＡｒｃｉｄａｅＺｈｅｎｇＪｉａｓｈｅｎｇ；ＷａｎｇＭｅｉｌｉｎ；ＧｕｏＤａｎｈｏｎｇ；ＸｕＸｉｍｉｎｄａｎｄＧａｏＱｉｎｇｌａｎＡｂｓｔｒａｃｔ：Ｂｙａｉｒ－...  相似文献   

Molecular analysis of complete ssu to lsu rdna sequence in the harmful dinoflagellatealexandrium tamarense (korean isolate, HY970328M)     

Jang-Seu Ki  Myung-Soo Han 《Ocean Science Journal》2005,40(3):43-54

New PCR primers (N=18) were designed for the isolation of complete SSU to LSU rDNA sequences from the dinoflagellateAlexandrium tamarense. Standard PCR, employing each primer set selected for amplifications of less than 1.5 kb, successfully amplified the expected rDNA regions of A. tamarense (Korean isolate, HY970328M). Complete SSU, LSU rDNAs and ITS sequences, including 5.8S rDNA, were recorded at 1,800 bp, 520 bp and 3,393 bp, respectively. The LSU rDNA sequence was the first report inAlexandrium genus. No intron was found in the LSU rRNA coding region. Twelve D-domains within the LSU rDNA were put together into 1,879 bp (44.4% G+C), and cores into 1514 bp (42.8% G+C). The core sequence was significantly different (0.0867 of genetic distance, 91% sequence similarity) in comparison withProrocentrum micans (GenBank access. no. X16108). The D2 region was the longest in length (300 bp) and highly variable among the 12 D-domains. In a phylogenetic analysis using complete LSU rDNA sequences of a variety of phytoplankton,A tamarense was clearly separated with high resolution against other species. The result suggests that the sequence may resolve the taxonomic ambiguities ofAlexandrium genus, particularly of the tamarensis complex.  相似文献   

耳鲍(Haliotis asinina)核糖体小亚基(18S rRNA)编码基因的克隆与序列分析   总被引：1，自引：0，他引：1       下载免费PDF全文

黄勃  方再光  刘均玲  周智  王小兵 《海洋与湖沼》2007,38(3):212-216

采用分子生物学的方法,对南海不同海区的两个地理群体耳鲍(Haliotis asinina)18S rRNA基因全长进行了克隆和序列分析,并将耳鲍18S rRNA基因的序列与NCBI数据库中已收录鲍的18SrRNA基因进行了比较。结果发现,南海耳鲍核糖体18S rRNA基因与耳鲍H.asinina isolate H11核糖体18S rRNA基因序列的相同率高达98%;同一地理群体内耳鲍核糖体18S rRNA基因序列完全一致;不同地理群体间耳鲍核糖体18S rRNA基因在碱基组成上的相似率为99%,仅在某些位点处发生了碱基替换,即腺嘌呤(T)被鸟嘌呤(G)替换;同时,将这两个不同群体中耳鲍的18S rRNA基因与泰国耳鲍18S rRNA基因序列进行比较分析发现,它们之间也只是发生了碱基替换。  相似文献   

Relationship between Shot-Induced Noise and Shot Interval in Seismic Refraction Experiments at Sea     

Herber  R.  Weigel  W.  Wong  H. K. 《Marine Geophysical Researches》1997,19(3):257-265

Seismic noise induced by the seismic source during continuous profiling reduces the signal-to-noise ratio and hence the data quality. This noise is largely dependent on the shot interval. In this paper, the noise amplitude of refraction seismic records from a special experiment is analysed as a function of the shot interval. An empirical exponential relationship between mean peak noise amplitude and shot interval is deduced. By increasing the shot interval, the induced noise can be minimized on all successive records. This results in an improvement of the data, predominantly the signal-to-noise ratio. Because the seismic signal and the shot-induced noise have nearly the same spectra, the chance of improving the signal-to-noise ratio by stacking is significantly reduced.  相似文献   

Seasonal methyltin and (3-dimethylsulphonio)propionate concentrations in leaf tissue of Spartina alterniflora of the Great Bay estuary (NH)     

James H. Weber  Marilyn R. Billings  Anne M. Falke 《Estuarine, Coastal and Shelf Science》1991,33(6)

Concentrations of total recoverable inorganic tin (TRISn), monomethyltin (MeSn³⁺), dimethyltin (Me₂Sn²⁺), trimethyltin (Me₃Sn⁺) and (3-dimethylsulphonio)propionate (DMSP) were determined in leaves of Spartina alterniflora from three sites in the Great Bay estuary (NH) from 8 May to 15 September 1989. Total methyltin concentration increased from 8·9 ng g⁻¹ (fresh weight) on 8 May to 472 ng g⁻¹ on 23 May, decreased to 52 ng g⁻¹ on 7 June and 16ng g⁻¹ on 20 June, and remained low until the last sample on 18 September. Statistical calculations showed that methyltin concentrations varied significantly with sampling week, but not with site. DMSP concentrations showed very different behaviour. During the same sampling period DMSP concentrations varied only from 7·5 to 26 μmol g⁻¹ (fresh weight). DMSP concentrations varied significantly for site, but not sampling week.  相似文献