长牡蛎碱性磷酸酶的分离纯化及部分性质研究   总被引：5，自引：1，他引：5  

李兴暖  韩雅莉  肖湘  邓瑞鹏  周小朋  管云雁  黄长江 《台湾海峡》2003,22(4):482-486

本文以长牡蛎为酶源提取材料,用正丁醇抽提,硫酸铵分级分离,DEAE-52和Sephadex G-100柱层析,得到一定纯度的碱性磷酸酶,提纯倍数为61.96,比活力达1.326U/mg.该酶的最适pH值9.6,最适温度38℃,初速度6.8μmol/(dm~3·min),米氏常数Km值为1.30mmol/dm~3.实验结果表明,Mg~(2+)对该酶有明显的激活作用;Zn~(2+)、Cu~(2+)、Hg~(2+)对该酶有明显的抑制作用.  相似文献   

Competition within the marine microalgae over the polar dark period in the Greenland Sea of high Arctic   总被引：2，自引：0，他引：2  

Zhang Qing  Rolf Gradinger  Zhou Qingsong 《海洋学报(英文版)》2003,22(2):233-242

Abstract-With the onset of winter, polar marine microalgae would have faced total darkness for aperiod of up to 6 months. A natural autumn community of Arctic sea ice microalgae was collected fordark survival experiments from the Greenland Sea during the ARKTIS-XI/2 Expedition of RV Po-larstern in October 1995. After a dark period of 161 days, species dominance in the algal assemblagehave changed from initially pennate diatoms to small phytoflagellates (<20μm). Over the entire darkperiod, the mean algal growth rate was-0.01 d~(-1). Nearly all diatom species had negative growthrates, while phytoflagellate abundance increased. Resting spore formation during the dark period was ob-served in less than 4.5% of all cells and only for dinoflagellates and the diatom Chaetoceros spp. We as-sume that facultative heterotrophy and energy storage are the main processes enabling survival during thedark Arctic winter. After an increase in light intensity, microalgal cells reacted with fast growth withindays. Phytoffa  相似文献   

Photoinduced toxicity single and binary mixtures of four polycyclic aromatic hydrocarbons to the marine diatom Skeletonema costatum   总被引：1，自引：0，他引：1  

MENG Wei  WANG Liping  ZHENG Binghui 《海洋学报(英文版)》2007,26(6):41-50

Diatom Skeletonema costatum Cleve is one of the main predominant phytoplankton species in the Changjiang Estuary in China. In order to provide some basic information for future assessment of the potential risk on phytoplankton communities in this estuary caused by polycyclic aromatic hydrocarbons(PAHs), this alga was selected as a representative to investigate the photoinduced toxicity of PAHs, in single and mixture. Four PAHs including three-ring phenanthrene and anthracene, four-ring fluoranthene and pyrene were tested in the laboratory. The single toxicity of each PAH on this microalga was compared with and without the simulated solar UV radiation. The results showed that this microalga was sensitive to PAH's photoinduced toxicity. Ratios of the 72 h median effect concentration obtained for fluorescent and UV light tests were about 8.4 for phenanthrene, 13.0 for anthracene, 6.5 for fluoranthene, and 5.7 for pyrene, indicating that UV light enhanced the PAH toxicity to this alga significantly. Under the fluorescent radiation (lacking UV), the dose-response curves based on chemical concentrations revealed that the order of toxic strength was fluoranthene greater than pyrene greater than anthracene greater than phenanthrene; while under the UV radiation (476 μW/cm2 for UVA, 6.5 μW/cm2 for UVB) it became fluoranthene approximately equaling anthracene greater than pyrene greater than phenanthrene, indicating that the UV light also changed its relative toxicity to this alga. The photoinduced toxicity of PAHs to the marine diatom S. costatum might be a synergistic effect of photosensitization reactions (e.g., generation of single-state oxygen) and photomodification (photooxidation and/or photolysis).The combined effects of six binary mixtures on the marine diatom S. costatum were investigated using the additive-index method. Four binary-mixtures (phenanthrene plus anthracene; phenanthrene plus pyrene; anthracene plus fluoranthene; anthracene plus pyrene) were found to be synergistic joint action mode, while two binary-mixtures (phenanthrene plus fluoranthene; fluoranthene plus pyrene) displayed antagonist, revealing a complex pattern of possible interactions. The combined action mode of PAHs might be related to various factors such as the mixture compounds, mixture ratios and test conditions, etc.  相似文献   

高压技术在深海沉积物兼性嗜压菌的筛选和鉴定中的应用     

游志勇  汤熙翔  肖湘 《台湾海峡》2007,26(4):555-561

通过自行改进的高压培养罐及高压设备,对深海沉积物进行可培养微生物的筛选,获得6株具有较强耐受压力的细菌.16SrDNA的测序结果表明这些细菌分别属于6个不同的菌属.压力生长试验的结果表明这6株细菌在40MPa的条件下仍然具有较强的生长能力,属于兼性嗜压菌.对不同压力下生长的细菌做显微镜检,结果显示,除了一株芽孢杆菌在40MPa下的菌体形态发生了明显的变化外,其它5株细菌在压力条件下的菌体的分裂均没有受到明显的影响.  相似文献   

大黄鱼倍性检测研究     

刘伟斌  谢湘筠  林向阳  曾志南  宋希坤 《台湾海峡》2006,25(1):48-53

于2001—2004年对人工诱导大黄鱼的不同发育阶段和不同组织进行倍性检测，胚胎眼泡期采用染色体计数法测定，鱼苗和成鱼阶段用流式细胞法，结果表明，胚胎染色体数目2n=48条为二倍体，3n=72条为三倍体．采用流式细胞法测定鱼苗和成鱼个体时，若受测样品的DNA相对含量值与已知对照组二倍体的比值约为1．5：1，其个体为三倍体；比值约为1：1，则为二倍体，无论用肌肉或尾鳍组织，皆可检测出其个体的倍性。  相似文献   

基于GIS的柘林湾生态信息系统     

黎裕成  陈志远  黄长江  谢文勇  杜虹 《台湾海峡》2005,24(2):216-222

柘林湾生态信息系统以ArcGIS为开发平台，采用客户端／服务器的体系结构，基于ArcObjects的组件式二次开发而成，实现了柘林湾的三维实景模拟、生态数学模型与GIS的有效集成、分析评价结果与地理空间数据相结合等功能，具有可视化、智能化、易扩展、安全实用等特点．该系统的建成．极大地提高了柘林湾生态信息的现代化管理水平，并为制定柘林湾的污染防治与生态环境保护策略提供了强有力的支持．  相似文献   

东海区近海强风预报模式     

杨良华 《海洋预报》1995,12(4):34-42

本文探讨东海区近海强风预报模式，首先介绍建立预报模式的思路和步骤，及使用说明，为了验证强风预报模式和判别条件，用１９８６、１９８７上日本传真天气图进行试报，准确和基本准确度为９１．１％，并给出了１９８９、１９９５年随船试验预报结果，准确和基本准确率为９０％以上。  相似文献   

A seismic refraction study of cretaceous oceanic lithosphere in the Northwest Pacific Basin     

Michel Bée  L. Dale Bibee 《Marine Geophysical Researches》1989,11(4):239-261

A seismic refraction study on old (110 Myr) lithosphere in the northwest Pacific Basin has placed constraints on crustal and uppermantle seismic structure of old oceanic lithosphere, and lithospheric aging processes. No significant lateral variation in structure other than azimuthally anisotropic mantle velocities was found, allowing the application of powerful amplitude modeling techniques. The anisotropy observed is in an opposite sense to that expected, suggesting the tectonic setting of the area may be more complex than originally thought. Upper crustal velocities are generally larger than for younger crust, supporting current theories of decreased porosity with crustal aging. However, there is no evidence for significant thickening of the oceanic crust with age, nor is there any evidence of a lower crustal layer of high or low velocity relative to the velocity of the rest of Layer 3. The compressional and shear wave velocities rule out a large component of serpentinization of mantle materials. The only evidence for a basal crustal layer of olivine gabbro cumulates is a 1.5 km thick Moho transition zone. In the slow direction of anisotropy, upper mantle velocities increase from 8.0 km s^-1 to 8.35 km s^-1 in the upper 15 km below the Moho. This increase is inconsistent with an homogeneous upper mantle and suggests that compositinal or phase changes occur near the Moho.  相似文献   

扇贝外寄生车轮虫（Trichodina　jadranica　Raabe，1958）的形态学研究　（原生动物：纤毛门：缘毛目）     

徐奎栋  类彦立  宋微波 《中国海洋大学学报(自然科学版)》1995,(3)

采用Ｗｉｌｂｅｒｔ蛋白银法及Ｃｈａｔｔｏｎ—Ｌｗｏｆｆ银浸法对一种附生于栉孔扇贝鳃丝上的寄生车轮虫进行了光镜水平下的研究。依据其形态学和纤毛图式特征，该种被鉴定为ＴｒｉｃｈｏｄｉｎａｊａｄｒａｎｉｃａＲａａｂｅ，１９５８．此为我国新记录，同时也是第一例有关外寄生于扇贝鳃表纤毛虫原生动物的报道。  相似文献   

开展我国海洋区域地质调查势在必行     

莫杰  刘守全 《海洋地质与第四纪地质》1993,13(2):99-108

本文概述了建国以来我国海洋地质调查与研究工作取得的丰硕成果。论述区域地质调查在海洋地质基础工作和未来海洋开发中的战略意义,介绍了国内外海洋区域地质调查的现状,对开展这项调查的可行性条件,以及进行1/100万海洋区域地质调查的项目内容、技术方法和提交成果等提出了设想和建议。  相似文献