Ontogeny of immune organs during early development stage of P.crocea     

XU Xiaojin  WENG Zhaohong  WANG Jun  SU Yongquan 《海洋学报(英文版)》2007,26(6):72-82

A histological and ultrastructural study was performed on the development of the head kidney, thymus, and spleen in Pseudosciaena crocea aging in range from just hatching to the 60th day after hatching (DAH). Head kidney was first present on the 3rd DAH. Primordial haemopoietic stem cells were first observed in the head kidney which rapidly differentiate into different cellular types. Progenitor spleen was present on the 4th DAH, located close to the gut, which soon became rich in blood capillaries, red blood cells and thrombocytes. The thymus was obvious on the 4th DAH, and was located on either side of the upper corner of the opercular cavity, closely under the membrance of the opercuhr cavity. The thymus was the last lymphoid organ to appear but showed a quick development. This organ seemed to originate from haemopoietic stem cells migrating from the head region of the kidney. The thymus consisted of outer thymocytic and inner epithelioid zones. There was no obvious demarcation between them, but both zones were visible. Small lymphocytes appeared, or lymphoid organs become lymphoid, in the sequence thymus, head kidney and spleen. Only a small number of lymphocytes appeared in the later stages. Non-specific systems may play an important role in the immunocompetence mechanisms of P. crocea during its early development stage.  相似文献   

海水养殖鱼类两种新贝尼登虫的RAPD分析   总被引：1，自引：0，他引：1  

张纹  王军  苏永全  丁少雄  杨文川 《台湾海峡》2001,20(4):519-523,T003

本文采用RAPD技术对寄生在福建沿海养殖大黄鱼和高体Shi鱼体上的Ji新贝尼登虫和梅氏新贝尼登虫的DNA多态性进行分析,结果显示：（1）两者具有高度的遗传相似性（83．1％－94．7％）,形态观察和分子检测结果相印证,两者有可能为同种异名;（2）不同海区鱼体表的寄生虫表现出一定的差异和分化,但相同宿主虫体的相似性都在90％以上：（3）虫体睾丸边缘光滑与否等形态特征应为种内变异,不宜作为分类标准。  相似文献   

湛江沿海大黄鱼种群的研究     

曹启华 《广东海洋大学学报》1998,(2)

徐闻和硇州两海区所产的大黄鱼,过去一直认为是同一种群—粤西种群。通过生物学测定,发现在体长组成、年龄组成、体重组成和年渔获量方面,两者有显著的差异,应分为徐闻种群和硇州种群。  相似文献   

海水网箱养殖大黄鱼病原菌研究   总被引：42，自引：3，他引：39  

林克冰  周宸  刘家富  周胜利  曾志南  陈木 《海洋科学》1999,23(4):58-62

从福建宁德地区患病大黄鱼肝、肾、脾等组织分离出３株病原菌,进行纯化培养、人工感染、药敏试验及菌种鉴定等研究,根据形态及生理生化特征,其中２株致病菌归为哈维氏弧菌,另一株为腐败假单胞菌。药敏实验的结果表明,氟哌酸、环丙氟哌酸、氯霉素、庆大霉素和复方新诺明等对致病菌有较强的抑制作用。  相似文献   

Characterization of ghrelin mRNA expression in fasting Larimichthys crocea juveniles     

Feng Liu  Xiaokang Lv  Tianqi Chu  Mengjie Wang  Wei Zhan  Bao Lou 《海洋学报(英文版)》2021,40(6):35-40

Larimichthys crocea is a marine fish species cultured in China.Short-term starvation is often applied to improve the quality of cultured L.crocea,and the expression of ghrelin in tissues of stomach,muscle,brain,intestines,liver,and kidney,involved in starvation response,under starvation conditions were studied to understand the effect of starvation on the expression of ghrelin in L.crocea juveniles.The ghrelin expression was tissue-specific,and expression was significantly higher in the stomach compared to other tissues(P0.01).Additionally,ghrelin expression in different tissues changed along with prolongation of fasting.In the stomach,ghrelin expression levels increased gradually at the beginning of the fast,and then declined after eight days of fasting.Gene expression in the brain and intestines increased at the beginning of the fast,and then decreased with longer fasting time.Interestingly,ghrelin expression declined at the beginning of the fast,then increased with longer fasting in the kidneys and muscles.These results suggest that ghrelin is involved in starvation response in L.crocea juveniles.This study provids insights into ghrelin function and an important reference for the development of reasonable feeding strategies for L.crocea juveniles.  相似文献   

大黄鱼皮质醇无创检测技术研究     

蔡晓芳  李庆昌  张晓林  历俊帆  刘贤德 《海洋通报》2019,38(5):569-573

皮质醇是衡量鱼类应激状况的重要参考指标,从血液中检测皮质醇的常规方法会对鱼造成严重伤害甚至致其死亡。本实验室开发了一种从水中检测皮质醇的技术,应用该技术比较了大黄鱼高温处理组(28℃)和对照组(20℃)血清与水样中皮质醇含量的变化,结果显示血清与水样皮质醇的检测结果具有高度的一致性,且相关性显著(P 0.05),说明通过测定水样中的皮质醇来代替血清中的皮质醇是可行的。为进一步验证该方法的效果,我们又比较了机械应激前后水样中皮质醇含量变化,结果显示机械胁迫也导致了皮质醇含量的显著升高(P 0.01)。本技术的建立可为大黄鱼养殖过程中应激状态的无创检测提供参考。  相似文献   

大黄鱼(Larimichthys crocea)耐环境因子试验及其遗传力的估计   总被引：1，自引：1，他引：0  

王晓清  王志勇  何湘蓉 《海洋与湖沼》2009,40(6):781-785

通过大黄鱼15个半同胞家系鱼苗对低盐、低溶氧和低pH值水体的抗性试验,采用半同胞组内相关法,对大黄鱼耐各环境因子遗传力进行了估计。结果表明,大黄鱼40日龄鱼苗在水体溶氧4.76—1.21mg/L时2.5—10h以内全部死亡,盐度8.05—9.03时1—30h内全部死亡,pH值4.49—5.2时1—8h内全部死亡;对耐低溶氧、低盐和低pH值的遗传力估计值分别为0.23、0.10和0.23,t检验表明对耐低溶氧和低pH值的遗传力估计达到显著水平,耐低盐遗传力的估计不显著。  相似文献   

人工育苗条件下的大黄鱼胚胎发育及其仔、稚鱼形态特征与生态习性的研究   总被引：20，自引：0，他引：20  

刘家富 《海洋科学》1999,23(6):61-65

连续观察人工育苗中同一批受精卵,在水温２３．２～２３．４℃,盐度２７．００～２７．８０条件下,经过２６ｈ３６ｍｉｎ孵出仔鱼,并继续观察仔,稚鱼发育过程中的形态特征与生态习性,还比较了不同年份与不同批次的观察资料,讨论了水温等环境条件变化的对胚胎发育的影响,以及仔,稚鱼器官发育与日龄,生长速度等关系。  相似文献   

溶藻弧菌脂多糖对大黄鱼的毒性与免疫保护性试验   总被引：10，自引：0，他引：10  

鄢庆枇  苏永全  王军  周永灿 《台湾海峡》2003,22(2):163-167

用酚水法从20dm3大黄鱼的病原菌--溶藻弧菌菌液分离出脂多糖(LPS)溶液380cm3,冻干后得到LPS粉末97.6mg.在注射剂量为0.2cm3不同浓度LPS溶液对大黄鱼肌肉注射毒性试验中,LPS注射浓度为0.5 mg/cm3 时, 5d内能引起大黄鱼死亡;当LPS浓度提高到2 mg/cm3时,大黄鱼的死亡率达到100%.以剂量为0.2 cm3、浓度为0.2 mg/cm3的LPS溶液对大黄鱼进行免疫注射,15d后用0.2cm3浓度为9×108 个/cm3的溶藻弧菌进行攻毒试验,40%的大黄鱼获得免疫保护.  相似文献   

渤海小黄鱼生殖力及其变化   总被引：9，自引：0，他引：9  

曾玲  金显仕  李富国  李显森  戴芳群 《海洋科学》2005,29(5):80-83

根据2004年5月在渤海进行的拖网调查资料,分析了小黄鱼(Pseudosciaenapolyatics)在经过近半世纪的捕捞后的生殖群体生殖力及其与体长、纯体质量的关系,并对小黄鱼生殖力的变化作了比较。结果表明,小黄鱼的个体绝对生殖力为14729粒±1360粒;个体绝对生殖力与叉长、纯体质量的关系变化较大;与1964年历史资料相比,相同体长组小黄鱼的个体绝对生殖力F和相对生殖力FL都显著增大(P<0.01)。小黄鱼的生殖力增加可能是小黄鱼在长期捕捞等外部环境影响下的遗传进化以及对其生存环境的适应性响应。  相似文献